LOCUS       Exported               17582 bp ds-DNA     circular SYN 07-AUG-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17582)
  AUTHORS   VIB Network
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 7, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17582
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     12..35
                     /label=101-019_pCHMWS-flag_fw16
                     /note="color: black; sequence: aactgagagggaaagatgttgaag; 
                     added: 2021-01-14"
     primer_bind     complement(53..74)
                     /label=92-033_Hs-chr3-flanking-seq-as
                     /note="color: black; sequence: gagtcgtaactaacggatgctg; 
                     added: 2021-01-14"
     primer_bind     96..118
                     /label=101-004_pCHMWS-flag_fw1
                     /note="color: black; sequence: ccatttactcctcaccctatgac; 
                     added: 2021-01-14"
     primer_bind     304..320
                     /label=c3f3
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     715..731
                     /label=c3f2
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     949..970
                     /label=101-005_pCHMWS-flag_fw2
                     /note="color: black; sequence: GTCAGCCTTCCTCATCTGCAGG; 
                     added: 2021-01-14"
     primer_bind     complement(1192..1211)
                     /label=pET-T7up
                     /note="color: black; sequence: CGGTGATGTCGGCGATATAG; added:
                     2021-01-07"
     primer_bind     complement(1411..1430)
                     /label=84-042_MLV_BB_as4
                     /note="color: black; sequence: gattgtactgagagtgcacc; added:
                     2021-01-14"
     primer_bind     complement(1512..1529)
                     /label=104-053_pCEP_L1RP-EGFP_bb2_FW
                     /note="color: black; sequence: TTGTCTGTAAGCGGATGC; added: 
                     2021-01-14"
     primer_bind     complement(1518..1537)
                     /label=rigt
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     1539..1558
                     /label=79-052_MFG_seq4
                     /note="color: black; sequence: GTCTCCGGGAGCTGCATGTG; added:
                     2021-01-14"
     primer_bind     1543..1565
                     /label=pGEX3
                     /note="color: black; sequence: CCGGGAGCTGCATGTGTCAGAGG; 
                     added: 2021-01-07"
     primer_bind     1547..1565
                     /label=GEX-R
                     /note="color: black; sequence: GAGCTGCATGTGTCAGAGG; added: 
                     2021-01-07"
     primer_bind     complement(1596..1620)
                     /label=98-052_seqPGEX_RV
                     /note="color: black; sequence: ATAGGCGTATCACGAGGCCCTTTCG; 
                     added: 2021-01-14"
     primer_bind     complement(1654..1673)
                     /label=pBR-F
                     /note="color: black; sequence: AGTGCCACCTGACGTCTAAG; added:
                     2021-01-07"
     misc_feature    1663..3652
                     /label=pUC backbone
                     /label=pUC\backbone
                     /label=nonstandard type:  cellular
                     /note="/vntifkey=82"
                     /note="color: #a6acb3"
     primer_bind     1674..1695
                     /label=101-006_pCHMWS-flag_fw3
                     /note="color: black; sequence: tttcggggaaatgtgcgcggaa; 
                     added: 2021-01-14"
     primer_bind     1685..1702
                     /label=94-006_pUC19_Rv_2000bp
                     /note="color: black; sequence: TGTGCGCGGAACCCCTAT; added: 
                     2021-01-14"
     primer_bind     complement(1686..1705)
                     /label=sCOS1F
                     /note="color: black; sequence: CAAATAGGGGTTCCGCGCAC; added:
                     2021-01-07"
     promoter        1689..1793
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     1755..1774
                     /label=74-040_MLVseq_rev2
                     /note="color: black; sequence: CCCTGATAAATGCTTCAATA; added:
                     2021-01-14"
     CDS             1794..2654
                     /codon_start=1
                     /label=bla AmpR
                     /label=bla\AmpR
                     /note="/vntifkey=4"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1809..1828)
                     /label=90-077_MSCV 24
                     /note="color: black; sequence: GGAATAAGGGCGACACGGAA; added:
                     2021-01-14"
     primer_bind     complement(1858..1879)
                     /label=78-007_p450_seq_ampR_start_fwd
                     /note="color: black; sequence: ACCAGCGTTTCTGGGTGAGCAA; 
                     added: 2021-01-14"
     primer_bind     complement(1925..1945)
                     /label=84-011_LEDGF s2
                     /note="color: black; sequence: Ctgttgagatccagttcgatg; 
                     added: 2021-01-14"
     primer_bind     1939..1959
                     /label=90-087_HIV2RODsqBBrv1_AB
                     /note="color: black; sequence: TCAACAGCGGTAAGATCCTTG; 
                     added: 2021-01-14"
     primer_bind     1943..1962
                     /label=85-033_psrs11_Amp_rev
                     /note="color: black; sequence: cagcggtaagatccttgaga; added:
                     2021-01-14"
     primer_bind     1945..1963
                     /label=84-043_AmpR_s
                     /note="color: black; sequence: Gcggtaagatccttgagag; added: 
                     2021-01-14"
     primer_bind     2023..2042
                     /label=85-034_psrs11_Amp2_rev
                     /note="color: black; sequence: cggtattatcccgtattgac; added:
                     2021-01-14"
     primer_bind     2047..2066
                     /label=84-008_psrs as1
                     /note="color: black; sequence: ggcaagagcaactcggtcgc; added:
                     2021-01-14"
     primer_bind     complement(2062..2084)
                     /label=84-040_MLV-BB_s4
                     /note="color: black; sequence: ctgagaatagtgtatgcggcgac; 
                     added: 2021-01-14"
     primer_bind     2062..2084
                     /label=84-041_MLV-BB_as3
                     /note="color: black; sequence: Gtcgccgcatacactattctcag; 
                     added: 2021-01-14"
     primer_bind     2113..2137
                     /label=99-057_huMeCP2 fw2
                     /note="color: black; sequence: CAGAAAAGCATCTTACGGATGGCAT; 
                     added: 2021-01-14"
     primer_bind     complement(2126..2146)
                     /label=99-021_pMT_seq_fw5-GFP
                     /note="color: black; sequence: CTTACTGTCATGCCATCCGTA; 
                     added: 2021-01-14"
     primer_bind     2192..2213
                     /label=84-017_LEDGF as2
                     /note="color: black; sequence: Caacttacttctgacaacgatc; 
                     added: 2021-01-14"
     primer_bind     2215..2234
                     /label=84-009_psrs as2
                     /note="color: black; sequence: gaggaccgaaggagctaacc; added:
                     2021-01-14"
     primer_bind     complement(2262..2281)
                     /label=84-007_psrs s2
                     /note="color: black; sequence: caacgatcaaggcgagttac; added:
                     2021-01-14"
     primer_bind     2265..2283
                     /label=100-058_AmpR Fw
                     /note="color: black; sequence: ACTCGCCTTGATCGTTGGG; added: 
                     2021-01-14"
     primer_bind     complement(2295..2314)
                     /label=74-028_MLVseq_fwd2
                     /note="color: black; sequence: TCGTTTGGTATGGCTTCATT; added:
                     2021-01-14"
     primer_bind     complement(2310..2329)
                     /label=90-076_MSCV 23
                     /note="color: black; sequence: GTGGTGTCACGCTCGTCGTT; added:
                     2021-01-14"
     primer_bind     complement(2330..2350)
                     /label=100-059_AmpR Rv
                     /note="color: black; sequence: GTTGCCATTGCTACAGGCATC; 
                     added: 2021-01-14"
     primer_bind     complement(2479..2503)
                     /label=99-089_pMT_seq_fw4_2fw-GFP
                     /note="color: black; sequence: TCACCGGCTCCAGATTTATCAGCAA; 
                     added: 2021-01-14"
     primer_bind     2481..2502
                     /label=101-007_pCHMWS-flag_fw4
                     /note="color: black; sequence: gctgataaatctggagccggtg; 
                     added: 2021-01-14"
     primer_bind     2575..2596
                     /label=78-008_p450_seq_ampR_end_rev
                     /note="color: black; sequence: CGACGGGGAGTCAGGCAACTAT; 
                     added: 2021-01-14"
     primer_bind     2610..2629
                     /label=74-041_MLVseq_rev3
                     /note="color: black; sequence: AGACAGATCGCTGAGATAGG; added:
                     2021-01-14"
     primer_bind     complement(2623..2642)
                     /label=85-032_psrs11_Amp2_s
                     /note="color: black; sequence: aatcagtgaggcacctatct; added:
                     2021-01-14"
     primer_bind     complement(2629..2648)
                     /label=GBE_primer2_Amp
                     /note="color: black; sequence: atgcttaatcagtgaggcac; added:
                     2021-01-07"
     primer_bind     complement(2637..2657)
                     /label=84-010_LEDGF s1
                     /note="color: black; sequence: Cagttaccaatgcttaatcag; 
                     added: 2021-01-14"
     primer_bind     2639..2667
                     /label=94-005_pUC19_Rv_1000bp
                     /note="color: black; sequence: 
                     GATTAAGCATTGGTAACTGTCAGACCAAG; added: 2021-01-14"
     primer_bind     complement(2645..2664)
                     /label=84-006_psrs s1
                     /note="color: black; sequence: ggtctgacagttaccaatgc; added:
                     2021-01-14"
     primer_bind     complement(2645..2664)
                     /label=84-044_AmpR_as
                     /note="color: black; sequence: ggtctgacagttaccaatgc; added:
                     2021-01-14"
     primer_bind     complement(2742..2762)
                     /label=85-031_psrs11_Amp_s
                     /note="color: black; sequence: gattttggtcatgagattatc; 
                     added: 2021-01-14"
     primer_bind     complement(2820..2844)
                     /label=90-075_MSCV 22
                     /note="color: black; sequence: GCAGAAAAAAAGGATCTCAAGAAGA; 
                     added: 2021-01-14"
     primer_bind     2829..2852
                     /label=101-001_pGEX-2TK-empty_fw4
                     /note="color: black; sequence: gatcctttttttctgcgcgtaatc; 
                     added: 2021-01-14"
     primer_bind     2883..2901
                     /label=100-009_pMeCP2G pc1121_pMeCP2mR-pc1083_8
                     /note="color: black; sequence: CCAGCGGTGGTTTGTTTGC; added: 
                     2021-01-14"
     primer_bind     2897..2916
                     /label=81-048_pSRS11_LTR_RV
                     /note="color: black; sequence: tttgccggatcaagagctac; added:
                     2021-01-14"
     primer_bind     complement(2967..2987)
                     /label=90-088_HIV2RODsqBBfw3_AB
                     /note="color: black; sequence: CGGCTACACTAGAAGGACAGT; 
                     added: 2021-01-14"
     primer_bind     2979..2998
                     /label=99-100_MeCP2-pc1208_MeCP2-T158M-pc1265_8
                     /note="color: black; sequence: GTGTAGCCGTAGTTAGGCCA; added:
                     2021-01-14"
     primer_bind     2993..3012
                     /label=80-032_SIV PCR1 rev
                     /note="color: black; sequence: AGGCCACCACTTCAAGAACT; added:
                     2021-01-14"
     primer_bind     complement(3042..3061)
                     /label=84-039_MLV-BB_s3
                     /note="color: black; sequence: Gccactggtaacaggattag; added:
                     2021-01-14"
     primer_bind     3059..3079
                     /label=80-023_NGS_MLV_as
                     /note="color: black; sequence: GGCTGCTGCCAGTGGCGATAA; 
                     added: 2021-01-14"
     primer_bind     complement(3151..3172)
                     /label=74-029_MLVseq_fwd3
                     /note="color: black; sequence: AAGCTGGGCTGTGTGCACGAAC; 
                     added: 2021-01-14"
     primer_bind     3186..3211
                     /label=94-004_pUC19_Rv_500bp
                     /note="color: black; sequence: TACACCGAACTGAGATACCTACAGCG; 
                     added: 2021-01-14"
     primer_bind     3192..3213
                     /label=100-010_pDest_GST-huMeCP2_fw3
                     /note="color: black; sequence: GAACTGAGATACCTACAGCGTG; 
                     added: 2021-01-14"
     primer_bind     complement(3210..3230)
                     /label=99-006_pMT_seq_fw4-GFP
                     /note="color: black; sequence: GGCGCTTTCTCATAGCTCACG; 
                     added: 2021-01-14"
     primer_bind     complement(3313..3332)
                     /label=90-074_MSCV 21
                     /note="color: black; sequence: AAGATACCAGGCGTTTCCCC; added:
                     2021-01-14"
     primer_bind     3315..3333
                     /label=101-008_pCHMWS-flag_fw5
                     /note="color: black; sequence: ggaaacgcctggtatcttt; added: 
                     2021-01-14"
     primer_bind     3363..3382
                     /label=78-018_p450_seq_origin
                     /note="color: black; sequence: cttgagcgtcgatttttgtg; added:
                     2021-01-14"
     primer_bind     3427..3447
                     /label=76-060_Fw_BamHI_gRNA
                     /note="color: black; sequence: 
                     AAAAAAGGATCCGGCCTTTTTACGGTTCCTGG; added: 2021-01-14"
     primer_bind     3427..3446
                     /label=ASK-FN2
                     /note="color: black; sequence: CGGCCTTTTTACGGTTCCTG; added:
                     2021-01-07"
     primer_bind     3436..3457
                     /label=94-003_pUC19_Rv_250bp
                     /note="color: black; sequence: TACGGTTCCTGGCCTTTTGCTG; 
                     added: 2021-01-14"
     primer_bind     3461..3480
                     /label=74-042_MLVseq_rev4
                     /note="color: black; sequence: TTTTGCTCACATGTTCTTTC; added:
                     2021-01-14"
     primer_bind     3465..3484
                     /label=85-038_mgpw2-reverse
                     /note="color: black; sequence: gctcacatgttctttcctgc; added:
                     2021-01-14"
     primer_bind     3481..3501
                     /label=66-074_MVG131867preCMV2_seq
                     /note="color: black; sequence: CTGCGTTATCCCCTGATTCTG; 
                     added: 2021-01-14"
     primer_bind     3481..3501
                     /label=66-075_MVG131868Gagpol_5_mid
                     /note="color: black; sequence: CTGCGTTATCCCCTGATTCTG; 
                     added: 2021-01-14"
     primer_bind     3504..3527
                     /label=81-063_pAC-V16-as1
                     /note="color: black; sequence: gataaccgtattaccgcctttgag; 
                     added: 2021-01-14"
     primer_bind     3519..3539
                     /label=84-045_MgpW_BB_s1
                     /note="color: black; sequence: Gcctttgagtgagctgatacc; 
                     added: 2021-01-14"
     primer_bind     3586..3603
                     /label=94-002_pUC19_Rv_100bp
                     /note="color: black; sequence: AAGCGGAAGAGCGCCCAA; added: 
                     2021-01-14"
     primer_bind     3628..3647
                     /label=79-100_seqAC1_V16_as10
                     /note="color: black; sequence: gttggccgattcattaatgc; added:
                     2021-01-14"
     enhancer        3708..3963
                     /label=SV40prom/enh
                     /note="/vntifkey=9"
                     /note="color: #a6acb3"
     primer_bind     complement(3827..3844)
                     /label=pSV40 L
                     /label=pSV40\L
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     3847..3866
                     /label=pSV40 R2
                     /label=pSV40\R2
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     3903..3921
                     /label=100-007_pMeCP2G pc1121_pMeCP2mR-pc1083_6
                     /note="color: black; sequence: TTTATGCAGAGGCCGAGGC; added: 
                     2021-01-14"
     primer_bind     complement(3923..3935)
                     /label=100-007_pMeCP2G pc1121_pMeCP2mR-pc1083_6
                     /note="color: black; sequence: TTTATGCAGAGGCCGAGGC; added: 
                     2021-01-14"
     primer_bind     3925..3944
                     /label=pSV40 R
                     /label=pSV40\R
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     3935..3956
                     /label=92-054_Gpt-s
                     /note="color: black; sequence: gagctattccagaagtagtgag; 
                     added: 2021-01-14"
     primer_bind     3944..3961
                     /label=SV40-Do
                     /note="color: black; sequence: CAGAAGTAGTGAGGAGGC; added: 
                     2021-01-07"
     primer_bind     4108..4131
                     /label=101-009_pCHMWS-flag_fw6
                     /note="color: black; sequence: cagatctctataatctcgcgcaac; 
                     added: 2021-01-14"
     CDS             4188..4645
                     /codon_start=1
                     /label=gpt (frameshift, ORF disrupted KpnI KO)
                     /label=gpt\(frameshift,\ORF\disrupted\KpnI\KO)
                     /note="/vntifkey=4"
                     /note="color: #993366"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLYRVRY
                     WRVNWVFVMSIPFVFPATITTTSASLKC*NAQKAMAKASSLLMTWWIPVVLRLRFVKCI
                     QKRTLSPSSQNRLVVRWLMTMLLISRKIPGLNSRGIWASYSSRQSPVA"
     primer_bind     4249..4266
                     /label=gpt
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     4728..4746
                     /label=84-046_MgpW_BB_s2
                     /note="color: black; sequence: Gctgcatccatgacacagg; added: 
                     2021-01-14"
     primer_bind     complement(4728..4746)
                     /label=84-049_MgpW_BB_as1
                     /note="color: black; sequence: Cctgtgtcatggatgcagc; added: 
                     2021-01-14"
     primer_bind     complement(4854..4874)
                     /label=92-055_Gpt-as
                     /note="color: black; sequence: gtgagggatggttttaccatc; 
                     added: 2021-01-14"
     primer_bind     4890..4911
                     /label=101-010_pCHMWS-flag_fw7
                     /note="color: black; sequence: aaccgtctgatgtggatctggc; 
                     added: 2021-01-14"
     polyA_signal    5047..5896
                     /label=SV40 polyA
                     /label=SV40\polyA
                     /note="/vntifkey=25"
                     /note="color: #a6acb3"
     primer_bind     complement(5196..5216)
                     /label=hGFP-R
                     /note="color: black; sequence: TCCCATTCATCAGTTCCATAG; 
                     added: 2021-01-07"
     primer_bind     complement(5206..5225)
                     /label=98-089_pMT_seq_fw2(GFP)
                     /note="color: black; sequence: CACCACTGCTCCCATTCATC; added:
                     2021-01-14"
     primer_bind     complement(5269..5287)
                     /label=SV40 PA L
                     /label=SV40\PA\L
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     5391..5410
                     /label=79-092_seqAC1_V16_as2
                     /note="color: black; sequence: gtttagtaatagaactcttg; added:
                     2021-01-14"
     primer_bind     5391..5410
                     /label=79-094_seqAC1_V16_as4
                     /note="color: black; sequence: gtttagtaatagaactcttg; added:
                     2021-01-14"
     primer_bind     complement(5411..5430)
                     /label=79-082_seqAC1_V16_s2
                     /note="color: black; sequence: ggtgtaaatagcaaagcaag; added:
                     2021-01-14"
     primer_bind     complement(5411..5430)
                     /label=79-084_seqAC1_V16_s4
                     /note="color: black; sequence: ggtgtaaatagcaaagcaag; added:
                     2021-01-14"
     primer_bind     complement(5656..5679)
                     /label=66-099_MVG131891p608_R_SalI
                     /note="color: black; sequence: 
                     CCCCGTCGACAATGTGGTATGGCTGATTATGATC; added: 2021-01-14"
     primer_bind     complement(5665..5687)
                     /label=B-017_62-030 MVG0121533pVpr-IN_SEQas
                     /note="color: black; sequence: CCTCTACAAATGTGGTATGGCTG; 
                     added: 2021-01-14"
     primer_bind     5700..5720
                     /label=101-011_pCHMWS-flag_fw8
                     /note="color: black; sequence: taaaaaacctcccacacctcc; 
                     added: 2021-01-14"
     primer_bind     complement(5704..5725)
                     /label=68-051_eGFP-Cr
                     /note="color: black; sequence: CAGGGGGAGGTGTGGGAGGTTT; 
                     added: 2021-01-14"
     primer_bind     5704..5725
                     /label=75-043_CR_compl
                     /note="color: black; sequence: AAACCTCCCACACCTCCCCCTG; 
                     added: 2021-01-14"
     primer_bind     complement(5704..5725)
                     /label=91-031_eGFP-Cr
                     /note="color: black; sequence: CAGGGGGAGGTGTGGGAGGTTT; 
                     added: 2021-01-14"
     primer_bind     5704..5720
                     /label=LTR combi_s
                     /label=LTR\combi_s
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(5715..5732)
                     /label=pFASTBacR
                     /note="color: black; sequence: TCAGGTTCAGGGGGAGGT; added: 
                     2021-01-07"
     primer_bind     5717..5735
                     /label=78-081_CX3CR1-GFP MUT FW
                     /note="color: black; sequence: CTCCCCCTGAACCTGAAAC; added: 
                     2021-01-14"
     primer_bind     complement(5719..5739)
                     /label=86-050_MVG011752Sv40_late_pA
                     /note="color: black; sequence: TTATGTTTCAGGTTCAGGGGG; 
                     added: 2021-01-14"
     primer_bind     complement(5727..5749)
                     /label=pee13.4seqrev
                     /note="color: black; sequence: TGCATTCATTTTATGTTTCAGGT; 
                     added: 2021-01-07"
     misc_feature    complement(5764..5787)
                     /label=HpaI PA
                     /label=HpaI\PA
                     /note="/vntifkey=21"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(5767..5789)
                     /label=EGFP-C1-R
                     /note="color: black; sequence: AACCATTATAAGCTGCAATAAAC; 
                     added: 2021-01-07"
     primer_bind     5837..5855
                     /label=87-076_pCEPseq_as
                     /note="color: black; sequence: tcactgcattctagttgtg; added: 
                     2021-01-14"
     primer_bind     5838..5858
                     /label=SV40
                     /note="color: black; sequence: CACTGCATTCTAGTTGTGGTT; 
                     added: 2021-01-07"
     primer_bind     5838..5856
                     /label=SV40 PA R
                     /label=SV40\PA\R
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(5842..5863)
                     /label=92-071_SV40-ori-as
                     /note="color: black; sequence: ggacaaaccacaactagaatgc; 
                     added: 2021-01-14"
     primer_bind     5860..5880
                     /label=84-047_MgpW_BB_s3
                     /note="color: black; sequence: Gtccaaactcatcaatgtatc; 
                     added: 2021-01-14"
     primer_bind     complement(5860..5880)
                     /label=84-050_MgpW_BB_as2
                     /note="color: black; sequence: Gatacattgatgagtttggac; 
                     added: 2021-01-14"
     primer_bind     complement(5867..5883)
                     /label=93-017_SV40 pA as
                     /note="color: black; sequence: ttacgccttaagatacattgatgag; 
                     added: 2021-01-14"
     primer_bind     complement(5869..5888)
                     /label=79-089_seqAC1_V16_s9
                     /note="color: black; sequence: catgataagatacattgatg; added:
                     2021-01-14"
     primer_bind     5869..5888
                     /label=79-099_seqAC1_V16_as9
                     /note="color: black; sequence: catcaatgtatcttatcatg; added:
                     2021-01-14"
     LTR             6065..6698
                     /label=5' LTR
                     /label=5'\LTR
                     /note="5' LTR"
                     /note="/vntifkey=19"
                     /note="color: #ffe4c4"
     primer_bind     6065..6084
                     /label=90-051_LTR NL4_3 FW1
                     /note="color: black; sequence: TGGAAGGGCTAATTCACTCC; added:
                     2021-01-14"
     primer_bind     complement(6255..6274)
                     /label=79-080_seqpoghas9
                     /note="color: black; sequence: ggtgtaacaagctggtgttc; added:
                     2021-01-14"
     primer_bind     complement(6430..6444)
                     /label=95-021_DHS1 rv
                     /note="color: black; sequence: CACACCTCCCTGGAAAGTC; added: 
                     2021-01-14"
     primer_bind     6484..6505
                     /label=101-012_pCHMWS-flag_fw9
                     /note="color: black; sequence: tcctgcatataagcagctgctt; 
                     added: 2021-01-14"
     primer_bind     complement(6494..6505)
                     /label=101-012_pCHMWS-flag_fw9
                     /note="color: black; sequence: tcctgcatataagcagctgctt; 
                     added: 2021-01-14"
     primer_bind     6497..6518
                     /label=88-003_nuc 1 fwd
                     /note="color: black; sequence: CAGCTGCTTTTTGCCTGTACTG; 
                     added: 2021-01-14"
     primer_bind     6547..6568
                     /label=95-022_Nuc 1 fw (mousseau)
                     /note="color: black; sequence: CTGGGAGCTCTCTGGCTAACTA; 
                     added: 2021-01-14"
     primer_bind     6561..6584
                     /label=83-027_Q-Alu nF out
                     /note="color: black; sequence: GCTAACTAGGGAACCCACTGCTTA; 
                     added: 2021-01-14"
     primer_bind     6561..6584
                     /label=87-092_Q-Alu nF out
                     /note="color: black; sequence: GCTAACTAGGGAACCCACTGCTTA; 
                     added: 2021-01-14"
     primer_bind     6581..6608
                     /label=70-083_454_MKL-3
                     /note="color: black; sequence: 
                     cttaagcctcaataaagcttgccttgag; added: 2021-01-14"
     primer_bind     6581..6608
                     /label=91-059_NGS LV pcr1 hiv ltr
                     /note="color: black; sequence: 
                     cttaagcctcaataaagcttgccttgag; added: 2021-01-14"
     primer_bind     6596..6616
                     /label=82-023_Q-Alu-F-in
                     /note="color: black; sequence: AGCTTGCCTTGAGTGCTTCAA; 
                     added: 2021-01-14"
     primer_bind     6596..6616
                     /label=83-028_Q-Alu-F-in
                     /note="color: black; sequence: AGCTTGCCTTGAGTGCTTCAA; 
                     added: 2021-01-14"
     primer_bind     6596..6616
                     /label=86-047_Q-Alu-F-in
                     /note="color: black; sequence: AGCTTGCCTTGAGTGCTTCAA; 
                     added: 2021-01-14"
     primer_bind     6596..6616
                     /label=87-093_Q-Alu-F-in
                     /note="color: black; sequence: AGCTTGCCTTGAGTGCTTCAA; 
                     added: 2021-01-14"
     primer_bind     complement(6629..6651)
                     /label=95-023_Nuc 1 rv (mousseau
                     /note="color: black; sequence: TTACCAGAGTCACACAACAGACG; 
                     added: 2021-01-14"
     primer_bind     6633..6660
                     /label=70-081_SK_Forward
                     /note="color: black; sequence: 
                     tgttgtgtgactctggtaactagagatc; added: 2021-01-14"
     primer_bind     6646..6667
                     /label=84-087_NGS_QC-LV_s2
                     /note="color: black; sequence: TGGTAACTAGAGATCCCTCAGA; 
                     added: 2021-01-14"
     primer_bind     6653..6684
                     /label=91-063_NGS LV R2
                     /note="color: black; sequence: 
                     tagagatccctcagacccttttagtcagtgtg; added: 2021-01-14"
     primer_bind     complement(6653..6684)
                     /label=91-064_NGS LV index primer
                     /note="color: black; sequence: 
                     cacactgactaaaagggtctgagggatctcta; added: 2021-01-14"
     primer_bind     complement(6656..6679)
                     /label=82-024_Q-Aul-R-in
                     /note="color: black; sequence: TGACTAAAAGGGTCTGAGGGATCT; 
                     added: 2021-01-14"
     primer_bind     complement(6656..6679)
                     /label=83-029_Q-Alu-R-in
                     /note="color: black; sequence: TGACTAAAAGGGTCTGAGGGATCT; 
                     added: 2021-01-14"
     primer_bind     complement(6656..6679)
                     /label=86-048_Q-Alu-R-in
                     /note="color: black; sequence: TGACTAAAAGGGTCTGAGGGATCT; 
                     added: 2021-01-14"
     primer_bind     complement(6656..6679)
                     /label=87-094_Q-Alu-R-in
                     /note="color: black; sequence: TGACTAAAAGGGTCTGAGGGATCT; 
                     added: 2021-01-14"
     primer_bind     complement(6663..6686)
                     /label=88-004_nuc 1 rev
                     /note="color: black; sequence: TCCACACTGACTAAAAGGGTCTGA; 
                     added: 2021-01-14"
     primer_bind     complement(6676..6698)
                     /label=90-053_LTR NL4_3 rev
                     /note="color: black; sequence: TGCTAGAGATTTTCCACACTGAC; 
                     added: 2021-01-14"
     primer_bind     6676..6697
                     /label=91-029_ngs QC LV s3
                     /note="color: black; sequence: gtcagtgtggaaaatctctagc; 
                     added: 2021-01-14"
     primer_bind     6678..6696
                     /label=86-070_seq_CA_s
                     /note="color: black; sequence: cagtgtggaaaatctctag; added: 
                     2021-01-14"
     primer_bind     6683..6703
                     /label=95-067_HSIV fw
                     /note="color: black; sequence: TGGAAAATCTCTAGCAGTGGC; 
                     added: 2021-01-14"
     misc_feature    6699..7206
                     /label=GAG
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     primer_bind     complement(6713..6731)
                     /label=LTR1
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     6736..6755
                     /label=99-065_OGH gag fw
                     /note="color: black; sequence: ccagaggagctctctcgacg; added:
                     2021-01-14"
     misc_feature    6750..6887
                     /label=packS
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     primer_bind     6764..6791
                     /label=77-033_FMS_multiple_spliced_F
                     /note="color: black; sequence: 
                     GGCTTGCTGAAGCGCGCACGGCAAGAGG; added: 2021-01-14"
     primer_bind     6801..6820
                     /label=95-024_Nuc 2 fw
                     /note="color: black; sequence: GCGACTGGTGAGTACGCCAA; added:
                     2021-01-14"
     misc_feature    6807..6810
                     /label=SD
                     /label=nonstandard type:  splicing_signal
                     /note="/vntifkey=38"
                     /note="color: #a6acb3"
     primer_bind     6860..6880
                     /label=84-048_MgpW_BB_s4
                     /note="color: black; sequence: gcgagagcgtcagtattaagc; 
                     added: 2021-01-14"
     primer_bind     complement(6860..6880)
                     /label=84-051_MgpW_BB_as3
                     /note="color: black; sequence: Gcttaatactgacgctctcgc; 
                     added: 2021-01-14"
     primer_bind     complement(6867..6886)
                     /label=84-088_NGS_QC-LV_as2
                     /note="color: black; sequence: TCCCCCGCTTAATACTGACG; added:
                     2021-01-14"
     misc_feature    6898..6899
                     /label=gag frame shift
                     /label=gag\frame\shift
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     primer_bind     complement(6908..6928)
                     /label=95-025_Nuc 2 rv
                     /note="color: black; sequence: CCCCTGGCCTTAACCGAATTT; 
                     added: 2021-01-14"
     primer_bind     complement(6958..6977)
                     /label=72-050_SK_Reverse2
                     /note="color: black; sequence: CTTTCCAGCTCCCTGCTTGCCCATAC; 
                     added: 2021-01-14"
     primer_bind     7008..7028
                     /label=gag1
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    7215..8073
                     /label=ENV
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     primer_bind     7288..7311
                     /label=101-013_pCHMWS-flag_fw10
                     /note="color: black; sequence: aattgaaccattaggagtagcacc; 
                     added: 2021-01-14"
     primer_bind     7308..7325
                     /label=84-085_NGS_QC-LV_s1
                     /note="color: black; sequence: CACCCACCAAGGCAAAGA; added: 
                     2021-01-14"
     protein_bind    7374..7578
                     /label=RRE
                     /note="/vntifkey=31"
                     /note="color: #31849b"
     primer_bind     complement(7411..7432)
                     /label=85-077_LV-TF-Env-as
                     /note="color: black; sequence: cgtcagcgtcattgacgctgcg; 
                     added: 2021-01-14"
     primer_bind     7483..7506
                     /label=97-037_Min CMV-BbvCI-s
                     /note="color: black; sequence: 
                     aaaaaagctgagggctattgaggcgcaaca; added: 2021-01-14"
     primer_bind     complement(7500..7517)
                     /label=84-086_NGS_QC-LV_as1
                     /note="color: black; sequence: GCAACAGATGCTGTTGCG; added: 
                     2021-01-14"
     primer_bind     7531..7550
                     /label=74-022_OGHenv_s7
                     /note="color: black; sequence: gggcatcaagcagctccagg; added:
                     2021-01-14"
     primer_bind     complement(7546..7565)
                     /label=84-052_MgpW_BB_as4
                     /note="color: black; sequence: Cagccaggattcttgcctgg; added:
                     2021-01-14"
     primer_bind     7813..7834
                     /label=Env1
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     7873..7895
                     /label=90-096_env C-term s
                     /note="color: black; sequence: cataatgatagtaggaggcttgg; 
                     added: 2021-01-14"
     primer_bind     7880..7903
                     /label=76-040_Env_PpuMI_s2
                     /note="color: black; sequence: atagtaggaggcttggtaggttta; 
                     added: 2021-01-14"
     primer_bind     7886..7910
                     /label=A-036_33-045 MVG09445-Env_Rev_f
                     /note="color: black; sequence: GGAGGCTTGGTAGGTTTAAGAATAG; 
                     added: 2021-01-14"
     primer_bind     7956..7976
                     /label=94-086_sgRNA Stein Aerts-s
                     /note="color: black; sequence: caccattatcgtttcagaccc; 
                     added: 2021-01-14"
     primer_bind     7956..7975
                     /label=85-037_mgpw_sense
                     /note="color: black; sequence: caccattatcgtttcagacc; added:
                     2021-01-14"
     primer_bind     7965..7986
                     /label=Fdet1
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     7965..7986
                     /label=75-041_Fdet1
                     /note="color: black; sequence: cgtttcagacccacctcccaac; 
                     added: 2021-01-14"
     primer_bind     7965..7983
                     /label=84-053_UCOE_s
                     /note="color: black; sequence: Cgtttcagacccacctccc; added: 
                     2021-01-14"
     misc_feature    7970..7972
                     /label=SA
                     /label=nonstandard type:  splicing_signal
                     /note="/vntifkey=38"
                     /note="color: #a6acb3"
     primer_bind     complement(8027..8053)
                     /label=84-084_msRNAtatrev antisense
                     /note="color: black; sequence: GGATCTGTCTCTGTCTCTCTCTCCACC;
                     added: 2021-01-14"
     primer_bind     8027..8049
                     /label=101-034_Benji_FW1
                     /note="color: black; sequence: ggtggagagagagacagagacag; 
                     added: 2021-01-14"
     misc_feature    8084..8273
                     /label=cPPT/CTS
                     /label=nonstandard type:  misc_marker
                     /note="/vntifkey=22"
                     /note="color: #a6acb3"
     primer_bind     8109..8148
                     /label=71-072_4.3_IN_F185H_sO
                     /note="color: black; sequence: 
                     CATCCACAATCATAAAAGAAAAGGGGGGATTGGGGGGTAC; added: 
                     2021-01-14"
     primer_bind     8133..8148
                     /label=71-071_4.3_IN_F185H_s
                     /note="color: black; sequence: GGGGATTGGGGGGTAC; added: 
                     2021-01-14"
     primer_bind     8134..8162
                     /label=82-083_mCPPT_sO
                     /note="color: black; sequence: 
                     aacttcaagcgcaagggcggtattggggggtacagtgcaggggaaag; added: 
                     2021-01-14"
     primer_bind     8137..8162
                     /label=82-081_cPPT-D_sO
                     /note="color: black; sequence: 
                     aacttcaagcgccgcggtggtattggggggtacagtgcaggggaaag; added: 
                     2021-01-14"
     primer_bind     8138..8162
                     /label=82-079_cPPT_s
                     /note="color: black; sequence: ttggggggtacagtgcaggggaaag; 
                     added: 2021-01-14"
     primer_bind     8144..8161
                     /label=101-014_pCHMWS-flag_fw11
                     /note="color: black; sequence: ggtacagtgcaggggaaa; added: 
                     2021-01-14"
     primer_bind     8167..8187
                     /label=70-097_cppt_S
                     /note="color: black; sequence: GTAGACATAATAGCAACAGAC; 
                     added: 2021-01-14"
     primer_bind     complement(8170..8191)
                     /label=76-039_cPPT_PpuMI_as
                     /note="color: black; sequence: gtatgtctgttgctattatgtc; 
                     added: 2021-01-14"
     primer_bind     complement(8172..8207)
                     /label=82-088_CTS_asO
                     /note="color: black; sequence: 
                     CTTAGTAATCTGCTTCTGTAATTCTTTAGTTTGTATGTCTGTTGCTATTATG; 
                     added: 2021-01-14"
     primer_bind     complement(8172..8205)
                     /label=82-086_CTS_as
                     /note="color: black; sequence: 
                     TAATTCTTTAGTTTGTATGTCTGTTGCTATTATG; added: 2021-01-14"
     primer_bind     complement(8180..8202)
                     /label=86-087_IN_core_BamHI_rev
                     /note="color: black; sequence: 
                     tacggatcctccgccTTCTTTAGTTTGTATGTCTGTTG; added: 2021-01-14"
     primer_bind     complement(8200..8225)
                     /label=Mut1
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     8203..8227
                     /label=86-088_IN_Ct_Xba_fwd
                     /note="color: black; sequence: 
                     tactctagacatgTTACAAAAACAAATTACAAAAATTC; added: 2021-01-14"
     primer_bind     8215..8253
                     /label=82-087_CTS_sO
                     /note="color: black; sequence: 
                     cagaagcagattactaagattcaaaattttcgggtttattacagggac; added: 
                     2021-01-14"
     primer_bind     8224..8253
                     /label=82-085_CTS_s
                     /note="color: black; sequence: 
                     attcaaaattttcgggtttattacagggac; added: 2021-01-14"
     primer_bind     complement(8244..8263)
                     /label=103-069_podocin_2_rev
                     /note="color: black; sequence: gatctctgctgtccctgtaa; added:
                     2021-01-14"
     promoter        8285..8797
                     /label=hCMV ie
                     /label=hCMV\ie
                     /note="/vntifkey=29"
                     /note="color: #ffffff"
     primer_bind     8285..8303
                     /label=99-093_MeCP2-pc1208_MeCP2-T158M-pc1265_1
                     /note="color: black; sequence: TGGAGTTCCGCGTTACATAA; added:
                     2021-01-14"
     primer_bind     8292..8318
                     /label=99-036_CAG S3
                     /note="color: black; sequence: 
                     GCCCATATATGGGCTAGCGCGTTACATAACTTACGGTAAATGGC; added: 
                     2021-01-14"
     primer_bind     8292..8318
                     /label=98-037_CAG S2
                     /note="color: black; sequence: 
                     AAAACTTAAGCTAGCGCGTTACATAACTTACGGTAAATGGC; added: 
                     2021-01-14"
     primer_bind     8292..8314
                     /label=98-007_CAG S1
                     /note="color: black; sequence: 
                     CTTAAGCTAGCGCGTTACATAACTTACGGTAAA; added: 2021-01-14"
     primer_bind     8306..8322
                     /label=103-077_CMV_s
                     /note="color: black; sequence: TACGGTAAATGGCCCGC; added: 
                     2021-01-14"
     primer_bind     8310..8327
                     /label=A-048_40-042 MVG1090CMVenh_s
                     /note="color: black; sequence: GTAAATGGCCCGCCTGGCATTAT; 
                     added: 2021-01-14"
     primer_bind     complement(8394..8413)
                     /label=Fdet4
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     8402..8418
                     /label=75-091_CMV1
                     /note="color: black; sequence: CCATTGACGTCAATGGGAG; added: 
                     2021-01-14"
     primer_bind     8402..8418
                     /label=93-015_CMV1
                     /note="color: black; sequence: CCATTGACGTCAATGGGAG; added: 
                     2021-01-14"
     primer_bind     8402..8418
                     /label=95-017_CMV1
                     /note="color: black; sequence: CCATTGACGTCAATGGGAG; added: 
                     2021-01-14"
     primer_bind     complement(8402..8417)
                     /label=75-091_CMV1
                     /note="color: black; sequence: CCATTGACGTCAATGGGAG; added: 
                     2021-01-14"
     primer_bind     complement(8402..8417)
                     /label=93-015_CMV1
                     /note="color: black; sequence: CCATTGACGTCAATGGGAG; added: 
                     2021-01-14"
     primer_bind     complement(8402..8417)
                     /label=95-017_CMV1
                     /note="color: black; sequence: CCATTGACGTCAATGGGAG; added: 
                     2021-01-14"
     primer_bind     complement(8431..8450)
                     /label=RV-L
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     8440..8459
                     /label=Fdet3
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     8484..8503
                     /label=84-012_LEDGF s3
                     /note="color: black; sequence: Cctattgacgtcaatgacgg; added:
                     2021-01-14"
     primer_bind     8500..8515
                     /label=103-077_CMV_s
                     /note="color: black; sequence: TACGGTAAATGGCCCGC; added: 
                     2021-01-14"
     primer_bind     8500..8511
                     /label=99-036_CAG S3
                     /note="color: black; sequence: 
                     GCCCATATATGGGCTAGCGCGTTACATAACTTACGGTAAATGGC; added: 
                     2021-01-14"
     primer_bind     8500..8511
                     /label=98-037_CAG S2
                     /note="color: black; sequence: 
                     AAAACTTAAGCTAGCGCGTTACATAACTTACGGTAAATGGC; added: 
                     2021-01-14"
     primer_bind     8503..8525
                     /label=A-048_40-042 MVG1090CMVenh_s
                     /note="color: black; sequence: GTAAATGGCCCGCCTGGCATTAT; 
                     added: 2021-01-14"
     primer_bind     8529..8548
                     /label=GBE_primer3_CMVenhancer
                     /note="color: black; sequence: cagtacatgaccttatggga; added:
                     2021-01-07"
     primer_bind     complement(8554..8578)
                     /label=A-049_40-043 MVG1091CMVenh_as
                     /note="color: black; sequence: AATACGTAGATGTACTGCCAAGTAG; 
                     added: 2021-01-14"
     primer_bind     complement(8577..8598)
                     /label=89-043_CMVenh PCR AS
                     /note="color: black; sequence: 
                     atttaaaagcttgtgccatggtaatagcgatgactaa; added: 2021-01-14"
     promoter        8598..8801
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        8599..8797
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(8670..8686)
                     /label=75-091_CMV1
                     /note="color: black; sequence: CCATTGACGTCAATGGGAG; added: 
                     2021-01-14"
     primer_bind     complement(8670..8686)
                     /label=93-015_CMV1
                     /note="color: black; sequence: CCATTGACGTCAATGGGAG; added: 
                     2021-01-14"
     primer_bind     complement(8670..8686)
                     /label=95-017_CMV1
                     /note="color: black; sequence: CCATTGACGTCAATGGGAG; added: 
                     2021-01-14"
     primer_bind     8671..8689
                     /label=CMV1
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     8671..8689
                     /label=75-091_CMV1
                     /note="color: black; sequence: CCATTGACGTCAATGGGAG; added: 
                     2021-01-14"
     primer_bind     8671..8689
                     /label=93-015_CMV1
                     /note="color: black; sequence: CCATTGACGTCAATGGGAG; added: 
                     2021-01-14"
     primer_bind     8671..8689
                     /label=95-017_CMV1
                     /note="color: black; sequence: CCATTGACGTCAATGGGAG; added: 
                     2021-01-14"
     primer_bind     8734..8754
                     /label=93-016_CMVie-sense
                     /note="color: black; sequence: Acaactccgccccattgacgc; 
                     added: 2021-01-14"
     primer_bind     8753..8771
                     /label=CMV-F
                     /note="color: black; sequence: GCAAATGGGCGGTAGGCGT; added: 
                     2021-01-07"
     primer_bind     8764..8785
                     /label=Ns
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(8764..8785)
                     /label=70-071_CMV173_as
                     /note="color: black; sequence: ACCTCCCACCGTACACGCCTAC; 
                     added: 2021-01-14"
     primer_bind     complement(8764..8785)
                     /label=85-061_CMV173_as
                     /note="color: black; sequence: ACCTCCCACCGTACACGCCTAC; 
                     added: 2021-01-14"
     primer_bind     8764..8785
                     /label=94-050_eGFP-Ns
                     /note="color: black; sequence: GTAGGCGTGTACGGTGGGAGGT; 
                     added: 2021-01-14"
     primer_bind     8768..8792
                     /label=pCEP4for
                     /note="color: black; sequence: GCGTGTACGGTGGGAGGTCTATATA; 
                     added: 2021-01-07"
     primer_bind     8781..8800
                     /label=EGFP-N1-F
                     /note="color: black; sequence: GAGGTCTATATAAGCAGAGC; added:
                     2021-01-07"
     primer_bind     8800..8819
                     /label=TRE-F
                     /note="color: black; sequence: CTCGTTTAGTGAACCGTCAG; added:
                     2021-01-07"
     primer_bind     8809..8872
                     /label=SEQ CHECKED FEB 2007
                     /label=SEQ\CHECKED\FEB\2007
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(8814..8831)
                     /label=CMV2
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     8871..8900
                     /label=Primer 1
                     /note="color: black; sequence: 
                     agccTCTAGAggtttagtgaaccgtcagatccg; added: 2020-12-07"
     primer_bind     8879..8895
                     /label=TRE-F
                     /note="color: black; sequence: CTCGTTTAGTGAACCGTCAG; added:
                     2021-01-07"
     primer_bind     8919..8938
                     /label=T7
                     /note="T7 promoter, forward primer"
                     /note="color: black; sequence: TAATACGACTCACTATAGGG"
     primer_bind     8919..8938
                     /label=76-024_pTOPO_T7_as
                     /note="color: black; sequence: taatacgactcactataggg; added:
                     2021-01-14"
     promoter        8919..8937
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     8919..8937
                     /label=T7cos1
                     /note="color: black; sequence: GCATAATACGACTCACTATAGG; 
                     added: 2021-01-07"
     misc_feature    8942..8954
                     /label=Kozak
                     /note="color: #a6acb3"
     CDS             8987..9007
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     primer_bind     8987..9003
                     /label=76-043_Cas9_BamHI Fwr
                     /note="color: black; sequence: 
                     TTTTTTGGATCCATGGCCCCAAAGAAGAAGCGGAA; added: 2021-01-14"
     CDS             9008..13108
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /note="This feature has 3 segments:
                        1:  9008 .. 11521 / #993366
                        2: 11522 .. 11524 / #cc99b2
                        3: 11525 .. 13108 / #993366"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     9010..9032
                     /label=76-089_Cas9_Seq1
                     /note="color: black; sequence: CAAGAAGTACAGCATCGGCCTGG; 
                     added: 2021-01-14"
     primer_bind     complement(9217..9237)
                     /label=104-064_Cas9_seq_rev
                     /note="color: black; sequence: CGGTTCTTCCGTCTGGTGTAT; 
                     added: 2021-01-14"
     primer_bind     9288..9311
                     /label=76-090_Cas9_Seq2
                     /note="color: black; sequence: ACAGCTTCTTCCACAGACTGGAAG; 
                     added: 2021-01-14"
     primer_bind     9473..9492
                     /label=GBE_primer5_nCas9
                     /note="color: black; sequence: gctctggcccacatgatcaa; added:
                     2021-01-07"
     primer_bind     9533..9553
                     /label=103-060 ABEmax-s3
                     /note="color: black; sequence: gacaacagcgacgtggacaag; 
                     added: 2021-01-07"
     primer_bind     9533..9553
                     /label=103-060_ABEmax-s3
                     /note="color: black; sequence: Gacaacagcgacgtggacaag; 
                     added: 2021-01-14"
     primer_bind     9628..9648
                     /label=76-091_Cas9_Seq3
                     /note="color: black; sequence: CAAGGCCATCCTGTCTGCCAG; 
                     added: 2021-01-14"
     primer_bind     10057..10078
                     /label=76-092_Cas9_Seq4
                     /note="color: black; sequence: CTTCGACCAGAGCAAGAACGGC; 
                     added: 2021-01-14"
     primer_bind     10126..10145
                     /label=103-061 ABEmax-s4
                     /note="color: black; sequence: gttcatcaagcccatcctgg; added:
                     2021-01-07"
     primer_bind     10126..10145
                     /label=103-061_ABEmax-s4
                     /note="color: black; sequence: Gttcatcaagcccatcctgg; added:
                     2021-01-14"
     primer_bind     10442..10461
                     /label=76-093_Cas9_Seq5
                     /note="color: black; sequence: GAAGTGGTGGACAAGGGCGC; added:
                     2021-01-14"
     primer_bind     10844..10867
                     /label=76-094 Cas9 Seq6
                     /note="color: black; sequence: gacattctggaagatatcgtgctg; 
                     added: 2021-01-07"
     primer_bind     10844..10867
                     /label=76-094_Cas9_Seq6
                     /note="color: black; sequence: GACATTCTGGAAGATATCGTGCTG; 
                     added: 2021-01-14"
     primer_bind     11090..11106
                     /label=GBE_primer7_nCas9
                     /note="color: black; sequence: caactgatccacgatgacag; added:
                     2021-01-07"
     primer_bind     11350..11368
                     /label=94-033 dCas9-KRAB
                     /note="color: black; sequence: gcggatcgaagagggcatc; added: 
                     2021-01-07"
     primer_bind     11350..11368
                     /label=94-033_dCas9-KRAB_Fw
                     /note="color: black; sequence: Gcggatcgaagagggcatc; added: 
                     2021-01-14"
     primer_bind     11365..11386
                     /label=94-011 dCas9_Fw10
                     /note="color: black; sequence: catcaaagagctgggcagccag; 
                     added: 2021-01-07"
     primer_bind     11686..11707
                     /label=76-095_Cas9_Seq7
                     /note="color: black; sequence: GAGAAAGTTCGACAATCTGACC; 
                     added: 2021-01-14"
     primer_bind     11957..11976
                     /label=94-012 dCas9_Fw11
                     /note="color: black; sequence: cacgacgcctacctgaacgc; added:
                     2021-01-07"
     primer_bind     complement(12424..12444)
                     /label=94-034 dCas9-KRAB_Rev
                     /note="color: black; sequence: gccaccaccagcacagaatag; 
                     added: 2021-01-07"
     primer_bind     12473..12494
                     /label=76-096_Cas9_Seq8
                     /note="color: black; sequence: CTGAAGAGTGTGAAAGAGCTGC; 
                     added: 2021-01-14"
     misc_feature    13109..13207
                     /label=Flexible linker
                     /note="color: #ffff99"
     misc_feature    13208..15272
                     /label=MLV-RT (+NLS)
                     /note="color: #00ccff"
     primer_bind     complement(13231..13250)
                     /label=74-048_MLVseq_rev10
                     /note="color: black; sequence: TTGAGGTCTCATGTAGCCGA; added:
                     2021-01-14"
     primer_bind     complement(13232..13250)
                     /label=74-048 MLVseq_rev10
                     /note="color: black; sequence: ttgaggtctcatgtagccg; added: 
                     2021-01-07"
     primer_bind     13546..13565
                     /label=74-035 MLVseq_fwd9
                     /note="color: black; sequence: ggatctgagagaagtcaaca; added:
                     2021-01-07"
     primer_bind     13546..13565
                     /label=74-035_MLVseq_fwd9
                     /note="color: black; sequence: GGATCTGAGAGAAGTCAACA; added:
                     2021-01-14"
     primer_bind     13587..13606
                     /label=MLV_GagPol_s1
                     /note="color: black; sequence: ccaccgtgcccaacccttac; added:
                     2021-01-07"
     primer_bind     complement(13587..13606)
                     /label=84-033 MLV_GagPol_as1
                     /note="color: black; sequence: gtaagggttgggcacggtgg; added:
                     2021-01-07"
     primer_bind     13711..13720
                     /label=81-063_pAC-V16-as1
                     /note="color: black; sequence: gataaccgtattaccgcctttgag; 
                     added: 2021-01-14"
     primer_bind     complement(14089..14108)
                     /label=74-047_MLVseq_rev9
                     /note="color: black; sequence: TTAGTTGTCGAGGGGTCTTC; added:
                     2021-01-14"
     primer_bind     complement(14317..14336)
                     /label=84-034_MLV_GagPol_as2
                     /note="color: black; sequence: Cgtagccctgcttctcgtcg; added:
                     2021-01-14"
     primer_bind     14395..14414
                     /label=74-034 MLVseq_fwd8
                     /note="color: black; sequence: caaaaagctagacccagtag; added:
                     2021-01-07"
     primer_bind     14395..14414
                     /label=74-034_MLVseq_fwd8
                     /note="color: black; sequence: CAAAAAGCTAGACCCAGTAG; added:
                     2021-01-14"
     primer_bind     14571..14590
                     /label=67-003 Gagpol_5_pr_s
                     /note="color: black; sequence: cccggatgactcactatcag; added:
                     2021-01-07"
     primer_bind     14571..14590
                     /label=67-003_MVG131897Gagpol_5_pr_s
                     /note="color: black; sequence: CCCGGATGACTCACTATCAG; added:
                     2021-01-14"
     primer_bind     complement(14937..14961)
                     /label=74-046 MLVseq_rev8
                     /note="color: black; sequence: cggctatcagtataaacatttagct; 
                     added: 2021-01-07"
     primer_bind     complement(14937..14961)
                     /label=74-046_MLVseq_rev8
                     /note="color: black; sequence: CGGCTATCAGTATAAACATTTAGCT; 
                     added: 2021-01-14"
     primer_bind     15113..15132
                     /label=77-069_MLV_IN_outside_s
                     /note="color: black; sequence: ccaggacatcaaaagggaca; added:
                     2021-01-14"
     primer_bind     complement(15279..15310)
                     /label=Primer 2
                     /note="color: black; sequence: 
                     cagaTGTACAttagactttcctcttcttcttgggct; added: 2020-12-07"
     CDS             15281..15301
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             15306..15312
                     /codon_start=1
                     /label=eYFP (3mut) - incl STOP
                     /label=eYFP
                     /note="/vntifkey=4"
                     /note="color: #ffff00"
                     /translation="YK"
     primer_bind     15320..15336
                     /label=97-003_Ires-MluI-s
                     /note="color: black; sequence: gaattcacgcgtgctctagcccaattc;
                     added: 2021-01-14"
     primer_bind     15345..15451
                     /label=79-003_BglII-stop-IRES-ApaI s
                     /note="color: black; sequence: 
                     gatcTAATAGcgccccccccccctaacgttactggccgaagccgcttggaataaggccg
                     gtgtgcgtttgtctatatgttattttccaccatattgccgtcttttggcaatgtgaggg
                     cc; added: 2021-01-14"
     primer_bind     complement(15368..15387)
                     /label=75-076_IRES_MCS
                     /note="color: black; sequence: ccttattccaagcggcttcg; added:
                     2021-01-14"
     primer_bind     complement(15368..15387)
                     /label=95-069_IRES_MCS
                     /note="color: black; sequence: ccttattccaagcggcttcg; added:
                     2021-01-14"
     primer_bind     complement(15391..15410)
                     /label=B-009_60-092 MVG121396-MARK1_Seq_Rev_1
                     /note="color: black; sequence: ACATATAGACAAACGCACAC; added:
                     2021-01-14"
     primer_bind     complement(15415..15438)
                     /label=98-012_Rv_LEDGF-PGAE-SFFV
                     /note="color: black; sequence: CCAAAAGACGGCAATATGGTGGAA; 
                     added: 2021-01-14"
     misc_feature    15445..15906
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     primer_bind     complement(15500..15518)
                     /label=89-028_pCH-STAT1-Seq Rv1
                     /note="color: black; sequence: gcattcctttggcgagagg; added: 
                     2021-01-14"
     primer_bind     complement(15507..15526)
                     /label=IREShygRn
                     /note="color: black; sequence: CAGACCTTGCATTCCTTTGG; added:
                     2021-01-07"
     primer_bind     15507..15526
                     /label=102-067_IRES_Fw
                     /note="color: black; sequence: ccaaaggaatgcaaggtctg; added:
                     2021-01-14"
     primer_bind     complement(15548..15567)
                     /label=98-088_pGAE_apaI_reverse
                     /note="color: black; sequence: caagaagcttccagaggaac; added:
                     2021-01-14"
     primer_bind     complement(15685..15704)
                     /label=76-086 IRES_R
                     /note="color: black; sequence: aactcacaacgtggcactgg; added:
                     2021-01-07"
     primer_bind     complement(15685..15704)
                     /label=76-086_MVG05687_IRES_R
                     /note="color: black; sequence: AACTCACAACGTGGCACTGG; added:
                     2021-01-14"
     primer_bind     complement(15685..15704)
                     /label=76-086_MVG05687_IRES_R (1)
                     /note="color: black; sequence: AACTCACAACGTGGCACTGG; added:
                     2021-01-14"
     primer_bind     complement(15685..15704)
                     /label=103-014_MVG05687_IRES_R
                     /note="color: black; sequence: AACTCACAACGTGGCACTGG; added:
                     2021-01-14"
     primer_bind     15772..15791
                     /label=A-078_56-061 MVG12880IRES_S
                     /note="color: black; sequence: GAAGGTACCCCATTGTATGG; added:
                     2021-01-14"
     primer_bind     15775..15799
                     /label=74-004_IRES_clon_fwd1
                     /note="color: black; sequence: 
                     Atgtcggtaccccattgtatgggatctgat; added: 2021-01-14"
     primer_bind     15820..15840
                     /label=pIRES-BF
                     /note="color: black; sequence: CTTTACATGTGTTTAGTCGAG; 
                     added: 2021-01-07"
     primer_bind     complement(15864..15882)
                     /label=98-087_pGAE_reverse
                     /note="color: black; sequence: aaccacgtccccgtggttc; added: 
                     2021-01-14"
     CDS             15914..16288
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVADEQD"
     primer_bind     16253..16271
                     /label=67-062_Zeo_FW
                     /note="color: black; sequence: aactgcgtgcacttcgtgg; added: 
                     2021-01-14"
     intron          16290..17195
                     /label=seq_d_sept11
                     /note="/vntifkey=15"
                     /note="color: #------"
     primer_bind     16290..16921
                     /label=SEQ CHECKED FEB 2007
                     /label=SEQ\CHECKED\FEB\2007
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(16301..16322)
                     /label=71-027_ptetMCS_as
                     /note="color: black; sequence: aatccagaggttgattatcggg; 
                     added: 2021-01-14"
     primer_bind     16304..16324
                     /label=84-015_LEDGF s6
                     /note="color: black; sequence: gataatcaacctctggattac; 
                     added: 2021-01-14"
     enhancer        16307..16908
                     /label=WPRE
                     /note="/vntifkey=9"
                     /note="color: #a6acb3"
     primer_bind     16361..16382
                     /label=WPRE
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(16364..16383)
                     /label=74-025_p_WPRE_as1
                     /note="color: black; sequence: ccacatagcgtaaaaggagc; added:
                     2021-01-14"
     primer_bind     complement(16399..16418)
                     /label=74-026_p_WPRE_as2
                     /note="color: black; sequence: caatagcatgatacaaaggc; added:
                     2021-01-14"
     primer_bind     complement(16457..16481)
                     /label=81-057_WPRE_PCR_AS
                     /note="color: black; sequence: CCTCATAAAGAGACAGCAACCAGGA; 
                     added: 2021-01-14"
     primer_bind     complement(16478..16496)
                     /label=101-042_WPRE_Rev
                     /note="color: black; sequence: caacgggccacaactcctc; added: 
                     2021-01-14"
     primer_bind     16491..16508
                     /label=104-044_WPRE for
                     /note="color: black; sequence: CCGTTGTCAGGCAACGTG; added: 
                     2021-01-14"
     primer_bind     16491..16508
                     /label=105-032_WPRE_Fw
                     /note="color: black; sequence: CCGTTGTCAGGCAACGTG; added: 
                     2021-01-14"
     primer_bind     16556..16575
                     /label=WPRE 2 Rev
                     /label=WPRE\2\Rev
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     16556..16575
                     /label=94-049_rev_primer_gBlock-repairtemplate
                     /note="color: black; sequence: Attgccaccacctgtcagct; added:
                     2021-01-14"
     primer_bind     complement(16556..16575)
                     /label=104-043_WPRE rev
                     /note="color: black; sequence: AGCTGACAGGTGGTGGCAAT; added:
                     2021-01-14"
     primer_bind     complement(16556..16575)
                     /label=A-035_27-99 MVG08579WPRE_Rev2
                     /note="color: black; sequence: AGCTGACAGGTGGTGGCAAT; added:
                     2021-01-14"
     primer_bind     complement(16624..16642)
                     /label=92-043_AAV_hsyn1-GCaMP_as4
                     /note="color: black; sequence: ggcaaggcaggcggcgatg; added: 
                     2021-01-14"
     CDS             16717..16956
                     /codon_start=1
                     /label=Chi-protein derived peptide (PRE)
                     /label=Chi-protein\derived\peptide\(PRE)
                     /note="/vntifkey=4"
                     /note="color: #993366"
                     /translation="MAARLCCHLDSARDVLLLRPFGPQSSGPSFPRPAAGSAASSASSP
                     SPSDESDLPLGRLPASGILSTGTFKTNDLQGSCRS"
     primer_bind     16734..16753
                     /label=66-096_MVG131888_WPRE_Seq_S1
                     /note="color: black; sequence: TTGCCACCTGGATTCTGCGC; added:
                     2021-01-14"
     primer_bind     16734..16753
                     /label=B-057_66-096 MVG131888_WPRE_Seq_S1
                     /note="color: black; sequence: TTGCCACCTGGATTCTGCGC; added:
                     2021-01-14"
     primer_bind     16784..16803
                     /label=92-032_WPRE-C-term-s
                     /note="color: black; sequence: ctcaatccagcggaccttcc; added:
                     2021-01-14"
     primer_bind     complement(16822..16841)
                     /label=84-021_LEDGF as6
                     /note="color: black; sequence: gcggaagaggccgcagagcc; added:
                     2021-01-14"
     misc_feature    16908..16983
                     /label=dNEF
                     /note="/vntifkey=21"
                     /note="color: #a6acb3"
     primer_bind     16942..16960
                     /label=Nef1
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(16943..16963)
                     /label=84-062_WPRE_as
                     /note="color: black; sequence: gtggctaagatctacagctgc; 
                     added: 2021-01-14"
     primer_bind     complement(16944..16961)
                     /label=Nef2
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(16946..16965)
                     /label=85-036_mgpw_3SIN-ref
                     /note="color: black; sequence: aagtggctaagatctacagc; added:
                     2021-01-14"
     LTR             16984..17219
                     /label=3'LTR-SIN
                     /note="3' LTR"
                     /note="/vntifkey=19"
                     /note="color: #ffe4c4"
     primer_bind     16986..17005
                     /label=90-051_LTR NL4_3 FW1
                     /note="color: black; sequence: TGGAAGGGCTAATTCACTCC; added:
                     2021-01-14"
     primer_bind     17033..17052
                     /label=101-012_pCHMWS-flag_fw9
                     /note="color: black; sequence: tcctgcatataagcagctgctt; 
                     added: 2021-01-14"
     primer_bind     complement(17041..17052)
                     /label=101-012_pCHMWS-flag_fw9
                     /note="color: black; sequence: tcctgcatataagcagctgctt; 
                     added: 2021-01-14"
     primer_bind     17044..17065
                     /label=88-003_nuc 1 fwd
                     /note="color: black; sequence: CAGCTGCTTTTTGCCTGTACTG; 
                     added: 2021-01-14"
     primer_bind     17094..17115
                     /label=95-022_Nuc 1 fw (mousseau)
                     /note="color: black; sequence: CTGGGAGCTCTCTGGCTAACTA; 
                     added: 2021-01-14"
     primer_bind     17108..17131
                     /label=83-027_Q-Alu nF out
                     /note="color: black; sequence: GCTAACTAGGGAACCCACTGCTTA; 
                     added: 2021-01-14"
     primer_bind     17108..17131
                     /label=87-092_Q-Alu nF out
                     /note="color: black; sequence: GCTAACTAGGGAACCCACTGCTTA; 
                     added: 2021-01-14"
     primer_bind     17128..17155
                     /label=70-083_454_MKL-3
                     /note="color: black; sequence: 
                     cttaagcctcaataaagcttgccttgag; added: 2021-01-14"
     primer_bind     17128..17155
                     /label=91-059_NGS LV pcr1 hiv ltr
                     /note="color: black; sequence: 
                     cttaagcctcaataaagcttgccttgag; added: 2021-01-14"
     polyA_site      17138..17143
                     /label=poly A
                     /label=poly\A
                     /note="/vntifkey=26"
                     /note="color: #a6acb3"
     primer_bind     17143..17163
                     /label=82-023_Q-Alu-F-in
                     /note="color: black; sequence: AGCTTGCCTTGAGTGCTTCAA; 
                     added: 2021-01-14"
     primer_bind     17143..17163
                     /label=83-028_Q-Alu-F-in
                     /note="color: black; sequence: AGCTTGCCTTGAGTGCTTCAA; 
                     added: 2021-01-14"
     primer_bind     17143..17163
                     /label=86-047_Q-Alu-F-in
                     /note="color: black; sequence: AGCTTGCCTTGAGTGCTTCAA; 
                     added: 2021-01-14"
     primer_bind     17143..17163
                     /label=87-093_Q-Alu-F-in
                     /note="color: black; sequence: AGCTTGCCTTGAGTGCTTCAA; 
                     added: 2021-01-14"
     primer_bind     complement(17176..17198)
                     /label=95-023_Nuc 1 rv (mousseau
                     /note="color: black; sequence: TTACCAGAGTCACACAACAGACG; 
                     added: 2021-01-14"
     primer_bind     17180..17207
                     /label=70-081_SK_Forward
                     /note="color: black; sequence: 
                     tgttgtgtgactctggtaactagagatc; added: 2021-01-14"
     primer_bind     17193..17214
                     /label=84-087_NGS_QC-LV_s2
                     /note="color: black; sequence: TGGTAACTAGAGATCCCTCAGA; 
                     added: 2021-01-14"
     primer_bind     17200..17231
                     /label=91-063_NGS LV R2
                     /note="color: black; sequence: 
                     tagagatccctcagacccttttagtcagtgtg; added: 2021-01-14"
     primer_bind     complement(17200..17231)
                     /label=91-064_NGS LV index primer
                     /note="color: black; sequence: 
                     cacactgactaaaagggtctgagggatctcta; added: 2021-01-14"
     primer_bind     complement(17203..17226)
                     /label=82-024_Q-Aul-R-in
                     /note="color: black; sequence: TGACTAAAAGGGTCTGAGGGATCT; 
                     added: 2021-01-14"
     primer_bind     complement(17203..17226)
                     /label=83-029_Q-Alu-R-in
                     /note="color: black; sequence: TGACTAAAAGGGTCTGAGGGATCT; 
                     added: 2021-01-14"
     primer_bind     complement(17203..17226)
                     /label=86-048_Q-Alu-R-in
                     /note="color: black; sequence: TGACTAAAAGGGTCTGAGGGATCT; 
                     added: 2021-01-14"
     primer_bind     complement(17203..17226)
                     /label=87-094_Q-Alu-R-in
                     /note="color: black; sequence: TGACTAAAAGGGTCTGAGGGATCT; 
                     added: 2021-01-14"
     primer_bind     complement(17210..17233)
                     /label=88-004_nuc 1 rev
                     /note="color: black; sequence: TCCACACTGACTAAAAGGGTCTGA; 
                     added: 2021-01-14"
     misc_feature    join(17220..17582,1..1063)
                     /label=Hs chr.3 flanking seq
                     /label=Hs\chr.3\flanking\seq
                     /label=nonstandard type:  cellular
                     /note="/vntifkey=82"
                     /note="color: #a6acb3"
     primer_bind     complement(17223..17245)
                     /label=90-053_LTR NL4_3 rev
                     /note="color: black; sequence: TGCTAGAGATTTTCCACACTGAC; 
                     added: 2021-01-14"
     primer_bind     17223..17244
                     /label=91-029_ngs QC LV s3
                     /note="color: black; sequence: gtcagtgtggaaaatctctagc; 
                     added: 2021-01-14"
     primer_bind     17225..17243
                     /label=86-070_seq_CA_s
                     /note="color: black; sequence: cagtgtggaaaatctctag; added: 
                     2021-01-14"
     primer_bind     17230..17247
                     /label=95-067_HSIV fw
                     /note="color: black; sequence: TGGAAAATCTCTAGCAGTGGC; 
                     added: 2021-01-14"
     primer_bind     17542..17558
                     /label=c3f4
                     /note="/vntifkey=28"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 acacaagagg aaactgagag ggaaagatgt tgaagtaatt ttcccacaat tacagcatcc
       61 gttagttacg actctatgat cttctgacac aaattccatt tactcctcac cctatgactc
      121 agtcgaatat atcaaagtta tggacattat gctaagtaac aaattaccct tttatatagt
      181 aaatactgag tagattgaga gaagaaattg tttgcaaacc tgaatagctt caagaagaag
      241 agaagtgagg ataagaataa cagttgtcat ttaacaagtt ttaacaagta acttggttag
      301 aaagggattc aaatgcataa agcaagggat aaatttttct ggcaacaaga ctatacaata
      361 taaccttaaa tatgacttca aataattgtt ggaacttgat aaaactaatt aaatattatt
      421 gaagattatc aatattataa atgtaattta cttttaaaaa gggaacatag aaatgtgtat
      481 cattagagta gaaaacaatc cttattatca caatttgtca aaacaagttt gttattaaca
      541 caagtagaat actgcattca attaagttga ctgcagattt tgtgttttgt taaaattaga
      601 aagagataac aacaatttga attattgaaa gtaacatgta aatagttcta catacgttct
      661 tttgacatct tgttcaatca ttgatcgaag ttctttatct tggaagaatt tgttccaaag
      721 actctgaaat aaggaaaaca atctattata tagtctcaca cctttgtttt acttttagtg
      781 atttcaattt aataatgtaa atggttaaaa tttattcttc tctgagatca tttcacattg
      841 cagatagaaa acctgagact ggggtaattt ttattaaaat ctaatttaat ctcagaaaca
      901 catctttatt ctaacatcaa tttttccagt ttgatattat catataaagt cagccttcct
      961 catctgcagg ttccacaaca aaaatccaac caactgtgga tcaaaaatat tgggaaaaaa
     1021 ttaaaaatag caatacaaca ataaaaaaat acaaatcaga aaaacagcac agtataacaa
     1081 ctttatttag catttacaat ctattaggta ttataagtaa tctagccaga tcctctacgc
     1141 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     1201 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     1261 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     1321 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     1381 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     1441 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     1501 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1561 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     1621 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1681 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1741 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1801 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1861 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1921 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1981 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2041 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2101 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2161 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2221 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2281 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2341 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2401 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2461 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2521 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2581 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2641 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2701 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2761 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2821 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2881 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2941 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3001 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3061 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3121 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3181 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3241 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3301 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3361 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3421 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3481 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3541 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3601 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     3661 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3721 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3781 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3901 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3961 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     4021 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     4081 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     4141 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     4201 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     4261 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     4321 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     4381 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     4441 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     4501 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     4561 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
     4621 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     4681 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     4741 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     4801 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
     4861 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     4921 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
     4981 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
     5041 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     5101 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     5161 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     5221 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     5281 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     5341 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     5401 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     5461 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     5521 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     5581 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     5641 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     5701 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     5761 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5821 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5881 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     5941 cccataccct attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt
     6001 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
     6061 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
     6121 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     6181 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     6241 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     6301 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     6361 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     6421 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     6481 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     6541 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6601 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6661 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     6721 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     6781 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     6841 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     6901 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     6961 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     7021 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     7081 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     7141 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     7201 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     7261 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     7321 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     7381 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     7441 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     7501 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     7561 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     7621 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     7681 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     7741 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     7801 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     7861 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     7921 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     7981 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     8041 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     8101 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     8161 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     8221 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gggcgataag
     8281 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     8341 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     8401 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     8461 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     8521 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     8581 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     8641 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     8701 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     8761 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     8821 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagaggt
     8881 ttagtgaacc gtcagatccg ctagagatcc gcggccgcta atacgactca ctatagggag
     8941 agccgccacc atgaaacgga cagccgacgg aagcgagttc gagtcaccaa agaagaagcg
     9001 gaaagtcgac aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc
     9061 cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga
     9121 ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc
     9181 cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat
     9241 ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca
     9301 cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt
     9361 cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag
     9421 aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc
     9481 ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag
     9541 cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa
     9601 ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag
     9661 cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg
     9721 aaacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc
     9781 cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct
     9841 ggcccagatc ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc
     9901 catcctgctg agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc
     9961 ctctatgatc aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt
    10021 gcggcagcag ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta
    10081 cgccggctac attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat
    10141 cctggaaaag atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct
    10201 gcggaagcag cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct
    10261 gcacgccatt ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa
    10321 gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa
    10381 cagcagattc gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga
    10441 ggaagtggtg gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga
    10501 taagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac
    10561 cgtgtataac gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt
    10621 cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt
    10681 gaccgtgaag cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga
    10741 aatctccggc gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa
    10801 aattatcaag gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat
    10861 cgtgctgacc ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta
    10921 tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg
    10981 gggcaggctg agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat
    11041 cctggatttc ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga
    11101 cgacagcctg acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag
    11161 cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca
    11221 gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat
    11281 cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga
    11341 gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca
    11401 ccccgtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg
    11461 gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga
    11521 cgctatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag
    11581 aagcgacaag aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat
    11641 gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa
    11701 tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag
    11761 acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat
    11821 gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa
    11881 gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa
    11941 caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa
    12001 aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg
    12061 gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta
    12121 cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa
    12181 gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga
    12241 ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga
    12301 ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct
    12361 gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt
    12421 ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag
    12481 tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat
    12541 cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc
    12601 taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga
    12661 actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc
    12721 cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt
    12781 ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag
    12841 agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga
    12901 taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg
    12961 agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac
    13021 caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg
    13081 gatcgacctg tctcagctgg gaggtgactc tggaggatct agcggaggat cctctggcag
    13141 cgagacacca ggaacaagcg agtcagcaac accagagagc agtggcggca gcagcggcgg
    13201 cagcagcacc ctaaatatag aagatgagta tcggctacat gagacctcaa aagagccaga
    13261 tgtttctcta gggtccacat ggctgtctga ttttcctcag gcctgggcgg aaaccggggg
    13321 catgggactg gcagttcgcc aagctcctct gatcatacct ctgaaagcaa cctctacccc
    13381 cgtgtccata aaacaatacc ccatgtcaca agaagccaga ctggggatca agccccacat
    13441 acagagactg ttggaccagg gaatactggt accctgccag tccccctgga acacgcccct
    13501 gctacccgtt aagaaaccag ggactaatga ttataggcct gtccaggatc tgagagaagt
    13561 caacaagcgg gtggaagaca tccaccccac cgtgcccaac ccttacaacc tcttgagcgg
    13621 gctcccaccg tcccaccagt ggtacactgt gcttgattta aaggatgcct ttttctgcct
    13681 gagactccac cccaccagtc agcctctctt cgcctttgag tggagagatc cagagatggg
    13741 aatctcagga caattgacct ggaccagact cccacagggt ttcaaaaaca gtcccaccct
    13801 gtttaatgag gcactgcaca gagacctagc agacttccgg atccagcacc cagacttgat
    13861 cctgctacag tacgtggatg acttactgct ggccgccact tctgagctag actgccaaca
    13921 aggtactcgg gccctgttac aaaccctagg gaacctcggg tatcgggcct cggccaagaa
    13981 agcccaaatt tgccagaaac aggtcaagta tctggggtat cttctaaaag agggtcagag
    14041 atggctgact gaggccagaa aagagactgt gatggggcag cctactccga agacccctcg
    14101 acaactaagg gagttcctag ggaaggcagg cttctgtcgc ctcttcatcc ctgggtttgc
    14161 agaaatggca gcccccctgt accctctcac caaaccgggg actctgttta attggggccc
    14221 agaccaacaa aaggcctatc aagaaatcaa gcaagctctt ctaactgccc cagccctggg
    14281 gttgccagat ttgactaagc cctttgaact ctttgtcgac gagaagcagg gctacgccaa
    14341 aggtgtccta acgcaaaaac tgggaccttg gcgtcggccg gtggcctacc tgtccaaaaa
    14401 gctagaccca gtagcagctg ggtggccccc ttgcctacgg atggtagcag ccattgccgt
    14461 actgacaaag gatgcaggca agctaaccat gggacagcca ctagtcattc tggcccccca
    14521 tgcagtagag gcactagtca aacaaccccc cgaccgctgg ctttccaacg cccggatgac
    14581 tcactatcag gccttgcttt tggacacgga ccgggtccag ttcggaccgg tggtagccct
    14641 gaacccggct acgctgctcc cactgcctga ggaagggctg caacacaact gccttgatat
    14701 cctggccgaa gcccacggaa cccgacccga cctaacggac cagccgctcc cagacgccga
    14761 ccacacctgg tacacggatg gaagcagtct cttacaagag ggacagcgta aggcgggagc
    14821 tgcggtgacc accgagaccg aggtaatctg ggctaaagcc ctgccagccg ggacatccgc
    14881 tcagcgggct gaactgatag cactcaccca ggccctaaag atggcagaag gtaagaagct
    14941 aaatgtttat actgatagcc gttatgcttt tgctactgcc catatccatg gagaaatata
    15001 cagaaggcgt gggtggctca catcagaagg caaagagatc aaaaataaag acgagatctt
    15061 ggccctacta aaagccctct ttctgcccaa aagacttagc ataatccatt gtccaggaca
    15121 tcaaaaggga cacagcgccg aggctagagg caaccggatg gctgaccaag cggcccgaaa
    15181 ggcagccatc acagagactc cagacacctc taccctcctc atagaaaatt catcaccctc
    15241 tggcggctca aaaagaaccg ccgacggcag cgaattcgag cccaagaaga agaggaaagt
    15301 ctaatgtaca agtaaactag tgctctagcc caattccgcc ccctcccccc cccctaacgt
    15361 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
    15421 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
    15481 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
    15541 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
    15601 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
    15661 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
    15721 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
    15781 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
    15841 gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
    15901 tgataatttc gaaatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
    15961 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
    16021 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
    16081 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
    16141 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
    16201 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
    16261 gcacttcgtg gccgacgagc aggactgaac gcgttgaatt cccgataatc aacctctgga
    16321 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
    16381 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
    16441 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
    16501 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
    16561 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
    16621 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
    16681 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
    16741 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
    16801 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
    16861 gacgagtcgg atctcccttt gggccgcctc cccgcatcgg gaattctgtc gactggtacc
    16921 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
    16981 gggactggaa gggctaattc actcccaacg aagacaaaat cgtcgagaga tgctgcatat
    17041 aagcagctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
    17101 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
    17161 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    17221 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
    17281 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattataa tagatttagc
    17341 aggaattgaa ctaggagtgg agcacacagg caaagctgca gaagtacttg gaagaagcca
    17401 ccagagatac tcacgattct gcacatacct ggctaatccc agatcctaag gattacatta
    17461 agtttactaa catttatata atgatttata gtttaaagta taaacttatc taatttacta
    17521 ttctgacaga tattaattaa tcctcaaata tcataagaga tgattactat tatccccatt
    17581 ta
//
