LOCUS       Exported                8990 bp ds-DNA     circular SYN 31-JAN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Mattijs Bulcaen
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 7, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     136..156
                     /label=93-016_CMVie-sense
                     /note="color: black; sequence: Acaactccgccccattgacgc; 
                     added: 2021-04-13"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
                     /note="color: black; sequence: CGCAAATGGGCGGTAGGCGTG"
     primer_bind     155..173
                     /label=CMV-Fa
                     /note="color: black; sequence: GCAAATGGGCGGTAGGCGT; added: 
                     2021-04-13"
     primer_bind     complement(166..187)
                     /label=70-071_CMV173_as
                     /note="color: black; sequence: ACCTCCCACCGTACACGCCTAC; 
                     added: 2021-04-13"
     primer_bind     complement(166..187)
                     /label=85-061_CMV173_as
                     /note="color: black; sequence: ACCTCCCACCGTACACGCCTAC; 
                     added: 2021-04-13"
     primer_bind     166..187
                     /label=94-050_eGFP-Ns
                     /note="color: black; sequence: GTAGGCGTGTACGGTGGGAGGT; 
                     added: 2021-04-13"
     primer_bind     170..194
                     /label=pCEP4for
                     /note="color: black; sequence: GCGTGTACGGTGGGAGGTCTATATA; 
                     added: 2021-04-13"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     246..267
                     /label=95-022_Nuc 1 fw (mousseau)
                     /note="color: black; sequence: CTGGGAGCTCTCTGGCTAACTA; 
                     added: 2021-04-13"
     primer_bind     260..283
                     /label=83-027_Q-Alu nF out
                     /note="color: black; sequence: GCTAACTAGGGAACCCACTGCTTA; 
                     added: 2021-04-13"
     primer_bind     260..283
                     /label=87-092_Q-Alu nF out
                     /note="color: black; sequence: GCTAACTAGGGAACCCACTGCTTA; 
                     added: 2021-04-13"
     primer_bind     280..307
                     /label=70-083_454_MKL-3
                     /note="color: black; sequence: 
                     cttaagcctcaataaagcttgccttgag; added: 2021-04-13"
     primer_bind     280..307
                     /label=91-059_NGS LV pcr1 hiv ltr
                     /note="color: black; sequence: 
                     cttaagcctcaataaagcttgccttgag; added: 2021-04-13"
     primer_bind     295..315
                     /label=82-023_Q-Alu-F-in
                     /note="color: black; sequence: AGCTTGCCTTGAGTGCTTCAA; 
                     added: 2021-04-13"
     primer_bind     295..315
                     /label=83-028_Q-Alu-F-in
                     /note="color: black; sequence: AGCTTGCCTTGAGTGCTTCAA; 
                     added: 2021-04-13"
     primer_bind     295..315
                     /label=86-047_Q-Alu-F-in
                     /note="color: black; sequence: AGCTTGCCTTGAGTGCTTCAA; 
                     added: 2021-04-13"
     primer_bind     295..315
                     /label=87-093_Q-Alu-F-in
                     /note="color: black; sequence: AGCTTGCCTTGAGTGCTTCAA; 
                     added: 2021-04-13"
     primer_bind     complement(328..350)
                     /label=95-023_Nuc 1 rv (mousseau
                     /note="color: black; sequence: TTACCAGAGTCACACAACAGACG; 
                     added: 2021-04-13"
     primer_bind     332..359
                     /label=70-081_SK_Forward
                     /note="color: black; sequence: 
                     tgttgtgtgactctggtaactagagatc; added: 2021-04-13"
     primer_bind     345..366
                     /label=84-087_NGS_QC-LV_s2
                     /note="color: black; sequence: TGGTAACTAGAGATCCCTCAGA; 
                     added: 2021-04-13"
     primer_bind     352..383
                     /label=91-063_NGS LV R2
                     /note="color: black; sequence: 
                     tagagatccctcagacccttttagtcagtgtg; added: 2021-04-13"
     primer_bind     complement(352..383)
                     /label=91-064_NGS LV index primer
                     /note="color: black; sequence: 
                     cacactgactaaaagggtctgagggatctcta; added: 2021-04-13"
     primer_bind     complement(355..378)
                     /label=82-024_Q-Aul-R-in
                     /note="color: black; sequence: TGACTAAAAGGGTCTGAGGGATCT; 
                     added: 2021-04-13"
     primer_bind     complement(355..378)
                     /label=83-029_Q-Alu-R-in
                     /note="color: black; sequence: TGACTAAAAGGGTCTGAGGGATCT; 
                     added: 2021-04-13"
     primer_bind     complement(355..378)
                     /label=86-048_Q-Alu-R-in
                     /note="color: black; sequence: TGACTAAAAGGGTCTGAGGGATCT; 
                     added: 2021-04-13"
     primer_bind     complement(355..378)
                     /label=87-094_Q-Alu-R-in
                     /note="color: black; sequence: TGACTAAAAGGGTCTGAGGGATCT; 
                     added: 2021-04-13"
     primer_bind     complement(362..385)
                     /label=88-004_nuc 1 rev
                     /note="color: black; sequence: TCCACACTGACTAAAAGGGTCTGA; 
                     added: 2021-04-13"
     primer_bind     complement(375..397)
                     /label=90-053_LTR NL4_3 rev
                     /note="color: black; sequence: TGCTAGAGATTTTCCACACTGAC; 
                     added: 2021-04-13"
     primer_bind     375..396
                     /label=91-029_ngs QC LV s3
                     /note="color: black; sequence: gtcagtgtggaaaatctctagc; 
                     added: 2021-04-13"
     primer_bind     377..395
                     /label=86-070_seq_CA_s
                     /note="color: black; sequence: cagtgtggaaaatctctag; added: 
                     2021-04-13"
     primer_bind     382..402
                     /label=95-067_HSIV fw
                     /note="color: black; sequence: TGGAAAATCTCTAGCAGTGGC; 
                     added: 2021-04-13"
     primer_bind     435..454
                     /label=99-065_OGH gag fw
                     /note="color: black; sequence: ccagaggagctctctcgacg; added:
                     2021-04-13"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     primer_bind     463..490
                     /label=77-033_FMS_multiple_spliced_F
                     /note="color: black; sequence: 
                     GGCTTGCTGAAGCGCGCACGGCAAGAGG; added: 2021-04-13"
     primer_bind     500..519
                     /label=95-024_Nuc 2 fw
                     /note="color: black; sequence: GCGACTGGTGAGTACGCCAA; added:
                     2021-04-13"
     primer_bind     559..579
                     /label=84-048_MgpW_BB_s4
                     /note="color: black; sequence: gcgagagcgtcagtattaagc; 
                     added: 2021-04-13"
     primer_bind     complement(559..579)
                     /label=84-051_MgpW_BB_as3
                     /note="color: black; sequence: Gcttaatactgacgctctcgc; 
                     added: 2021-04-13"
     primer_bind     complement(566..585)
                     /label=84-088_NGS_QC-LV_as2
                     /note="color: black; sequence: TCCCCCGCTTAATACTGACG; added:
                     2021-04-13"
     primer_bind     complement(607..627)
                     /label=95-025_Nuc 2 rv
                     /note="color: black; sequence: CCCCTGGCCTTAACCGAATTT; 
                     added: 2021-04-13"
     primer_bind     complement(657..676)
                     /label=72-050_SK_Reverse2
                     /note="color: black; sequence: CTTTCCAGCTCCCTGCTTGCCCATAC; 
                     added: 2021-04-13"
     primer_bind     987..1010
                     /label=101-013_pCHMWS-flag_fw10
                     /note="color: black; sequence: aattgaaccattaggagtagcacc; 
                     added: 2021-04-13"
     primer_bind     1007..1024
                     /label=84-085_NGS_QC-LV_s1
                     /note="color: black; sequence: CACCCACCAAGGCAAAGA; added: 
                     2021-04-13"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     primer_bind     complement(1110..1131)
                     /label=85-077_LV-TF-Env-as
                     /note="color: black; sequence: cgtcagcgtcattgacgctgcg; 
                     added: 2021-04-13"
     primer_bind     1182..1205
                     /label=97-037_Min CMV-BbvCI-s
                     /note="color: black; sequence: 
                     aaaaaagctgagggctattgaggcgcaaca; added: 2021-04-13"
     primer_bind     complement(1199..1216)
                     /label=84-086_NGS_QC-LV_as1
                     /note="color: black; sequence: GCAACAGATGCTGTTGCG; added: 
                     2021-04-13"
     primer_bind     1230..1249
                     /label=74-022_OGHenv_s7
                     /note="color: black; sequence: gggcatcaagcagctccagg; added:
                     2021-04-13"
     primer_bind     complement(1245..1264)
                     /label=84-052_MgpW_BB_as4
                     /note="color: black; sequence: Cagccaggattcttgcctgg; added:
                     2021-04-13"
     primer_bind     1572..1594
                     /label=90-096_env C-term s
                     /note="color: black; sequence: cataatgatagtaggaggcttgg; 
                     added: 2021-04-13"
     primer_bind     1579..1602
                     /label=76-040_Env_PpuMI_s2
                     /note="color: black; sequence: atagtaggaggcttggtaggttta; 
                     added: 2021-04-13"
     primer_bind     1585..1609
                     /label=A-036_33-045 MVG09445-Env_Rev_f
                     /note="color: black; sequence: GGAGGCTTGGTAGGTTTAAGAATAG; 
                     added: 2021-04-13"
     primer_bind     1655..1675
                     /label=94-086_sgRNA Stein Aerts-s
                     /note="color: black; sequence: caccattatcgtttcagaccc; 
                     added: 2021-04-13"
     primer_bind     1655..1674
                     /label=85-037_mgpw_sense
                     /note="color: black; sequence: caccattatcgtttcagacc; added:
                     2021-04-13"
     primer_bind     1664..1685
                     /label=75-041_Fdet1
                     /note="color: black; sequence: cgtttcagacccacctcccaac; 
                     added: 2021-04-13"
     primer_bind     1664..1682
                     /label=84-053_UCOE_s
                     /note="color: black; sequence: Cgtttcagacccacctccc; added: 
                     2021-04-13"
     primer_bind     complement(1726..1752)
                     /label=84-084_msRNAtatrev antisense
                     /note="color: black; sequence: GGATCTGTCTCTGTCTCTCTCTCCACC;
                     added: 2021-04-13"
     primer_bind     1726..1748
                     /label=101-034_Benji_FW1
                     /note="color: black; sequence: ggtggagagagagacagagacag; 
                     added: 2021-04-13"
     primer_bind     1813..1852
                     /label=71-072_4.3_IN_F185H_sO
                     /note="color: black; sequence: 
                     CATCCACAATCATAAAAGAAAAGGGGGGATTGGGGGGTAC; added: 
                     2021-04-13"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     1837..1852
                     /label=71-071_4.3_IN_F185H_s
                     /note="color: black; sequence: GGGGATTGGGGGGTAC; added: 
                     2021-04-13"
     primer_bind     1838..1866
                     /label=82-083_mCPPT_sO
                     /note="color: black; sequence: 
                     aacttcaagcgcaagggcggtattggggggtacagtgcaggggaaag; added: 
                     2021-04-13"
     primer_bind     1841..1866
                     /label=82-081_cPPT-D_sO
                     /note="color: black; sequence: 
                     aacttcaagcgccgcggtggtattggggggtacagtgcaggggaaag; added: 
                     2021-04-13"
     primer_bind     1842..1866
                     /label=82-079_cPPT_s
                     /note="color: black; sequence: ttggggggtacagtgcaggggaaag; 
                     added: 2021-04-13"
     primer_bind     1848..1865
                     /label=101-014_pCHMWS-flag_fw11
                     /note="color: black; sequence: ggtacagtgcaggggaaa; added: 
                     2021-04-13"
     primer_bind     1871..1891
                     /label=70-097_cppt_S
                     /note="color: black; sequence: GTAGACATAATAGCAACAGAC; 
                     added: 2021-04-13"
     primer_bind     complement(1874..1895)
                     /label=76-039_cPPT_PpuMI_as
                     /note="color: black; sequence: gtatgtctgttgctattatgtc; 
                     added: 2021-04-13"
     primer_bind     complement(1876..1911)
                     /label=82-088_CTS_asO
                     /note="color: black; sequence: 
                     CTTAGTAATCTGCTTCTGTAATTCTTTAGTTTGTATGTCTGTTGCTATTATG; 
                     added: 2021-04-13"
     primer_bind     complement(1876..1909)
                     /label=82-086_CTS_as
                     /note="color: black; sequence: 
                     TAATTCTTTAGTTTGTATGTCTGTTGCTATTATG; added: 2021-04-13"
     primer_bind     complement(1884..1906)
                     /label=86-087_IN_core_BamHI_rev
                     /note="color: black; sequence: 
                     tacggatcctccgccTTCTTTAGTTTGTATGTCTGTTG; added: 2021-04-13"
     primer_bind     1907..1931
                     /label=86-088_IN_Ct_Xba_fwd
                     /note="color: black; sequence: 
                     tactctagacatgTTACAAAAACAAATTACAAAAATTC; added: 2021-04-13"
     primer_bind     1919..1957
                     /label=82-087_CTS_sO
                     /note="color: black; sequence: 
                     cagaagcagattactaagattcaaaattttcgggtttattacagggac; added: 
                     2021-04-13"
     primer_bind     1928..1957
                     /label=82-085_CTS_s
                     /note="color: black; sequence: 
                     attcaaaattttcgggtttattacagggac; added: 2021-04-13"
     primer_bind     complement(1948..1967)
                     /label=103-069_podocin_2_rev
                     /note="color: black; sequence: gatctctgctgtccctgtaa; added:
                     2021-04-13"
     primer_bind     1987..2001
                     /label=Primer 2 (1)
                     /note="color: black; sequence: aaaaaaGCTAGCacctcgagcggccca;
                     added: 2021-01-27"
     misc_feature    2014..2050
                     /label=MB_tEvopreQ1_motif
                     /note="RNA motif with tertiary structure to protect pegRNA 
                     3'extension from degradation by endogenous nucleases. 
                     (Nelson et al., Nature biotechnology, 2021=David Liu lab)"
                     /note="color: #ff6600"
     misc_RNA        complement(2083..2158)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
                     /note="color: #00ccff; direction: LEFT"
     misc_feature    2159..2178
                     /label=MB_N1303K-peg13-spacer
                     /note="color: #99ccff"
     promoter        complement(2188..2428)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(2238..2257)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
                     /note="color: black; sequence: GACTATCATATGCTTACCGT"
     primer_bind     complement(2408..2428)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
                     /note="color: black; sequence: GAGGGCCTATTTCCCATGATT"
     primer_bind     complement(2409..2428)
                     /label=105-030_GFP_TK_for
                     /note="color: black; sequence: 
                     AAAAcGGCGCGCCgagggcctatttcccatgat; added: 2021-04-13"
     promoter        2547..2758
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(2580..2599)
                     /label=99-071_gRNA_Sequencing_rev
                     /note="color: black; sequence: gccgacccctccccccaact; added:
                     2021-04-13"
     primer_bind     2607..2622
                     /label=79-067_seqpoghs4
                     /note="color: black; sequence: tgaaccggtgcctagagaag; added:
                     2021-04-13"
     regulatory      2771..2780
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             2795..2851
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 2848"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2852..3448
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2999..3019)
                     /label=77-077_Puro_rv
                     /note="color: black; sequence: GACGCGCGTGAGGAAGAGTTC; 
                     added: 2021-04-13"
     primer_bind     3169..3188
                     /label=83-002_Puro_intern_S
                     /note="color: black; sequence: ACAGATGGAAGGCCTCCTGG; added:
                     2021-04-13"
     primer_bind     3345..3365
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
                     /note="color: black; sequence: GCAACCTCCCCTTCTACGAGC"
     primer_bind     complement(3430..3448)
                     /label=94-026_Puro_rev
                     /note="color: black; sequence: 
                     aaaaaagcggccgctcaggcaccgggcttgcgg; added: 2021-04-13"
     misc_feature    3464..4052
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     3464..3481
                     /label=84-015_LEDGF s6
                     /note="color: black; sequence: gataatcaacctctggattac; 
                     added: 2021-04-13"
     primer_bind     complement(3517..3537)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     primer_bind     complement(3521..3540)
                     /label=74-025_p_WPRE_as1
                     /note="color: black; sequence: ccacatagcgtaaaaggagc; added:
                     2021-04-13"
     primer_bind     complement(3556..3575)
                     /label=74-026_p_WPRE_as2
                     /note="color: black; sequence: caatagcatgatacaaaggc; added:
                     2021-04-13"
     primer_bind     complement(3614..3638)
                     /label=81-057_WPRE_PCR_AS
                     /note="color: black; sequence: CCTCATAAAGAGACAGCAACCAGGA; 
                     added: 2021-04-13"
     primer_bind     complement(3635..3653)
                     /label=101-042_WPRE_Rev
                     /note="color: black; sequence: caacgggccacaactcctc; added: 
                     2021-04-13"
     primer_bind     3648..3665
                     /label=104-044_WPRE for
                     /note="color: black; sequence: CCGTTGTCAGGCAACGTG; added: 
                     2021-04-13"
     primer_bind     3648..3665
                     /label=105-032_WPRE_Fw
                     /note="color: black; sequence: CCGTTGTCAGGCAACGTG; added: 
                     2021-04-13"
     primer_bind     3713..3732
                     /label=94-049_rev_primer_gBlock-repairtemplate
                     /note="color: black; sequence: Attgccaccacctgtcagct; added:
                     2021-04-13"
     primer_bind     complement(3713..3732)
                     /label=104-043_WPRE rev
                     /note="color: black; sequence: AGCTGACAGGTGGTGGCAAT; added:
                     2021-04-13"
     primer_bind     complement(3713..3732)
                     /label=A-035_27-99 MVG08579WPRE_Rev2
                     /note="color: black; sequence: AGCTGACAGGTGGTGGCAAT; added:
                     2021-04-13"
     primer_bind     complement(3781..3799)
                     /label=92-043_AAV_hsyn1-GCaMP_as4
                     /note="color: black; sequence: ggcaaggcaggcggcgatg; added: 
                     2021-04-13"
     primer_bind     3891..3910
                     /label=66-096_MVG131888_WPRE_Seq_S1
                     /note="color: black; sequence: TTGCCACCTGGATTCTGCGC; added:
                     2021-04-13"
     primer_bind     3891..3910
                     /label=B-057_66-096 MVG131888_WPRE_Seq_S1
                     /note="color: black; sequence: TTGCCACCTGGATTCTGCGC; added:
                     2021-04-13"
     CDS             complement(3935..3946)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3934"
                     /translation="IEGR"
     primer_bind     3941..3960
                     /label=92-032_WPRE-C-term-s
                     /note="color: black; sequence: ctcaatccagcggaccttcc; added:
                     2021-04-13"
     primer_bind     complement(3979..3998)
                     /label=84-021_LEDGF as6
                     /note="color: black; sequence: gcggaagaggccgcagagcc; added:
                     2021-04-13"
     primer_bind     complement(4081..4101)
                     /label=84-062_WPRE_as
                     /note="color: black; sequence: gtggctaagatctacagctgc; 
                     added: 2021-04-13"
     primer_bind     complement(4084..4103)
                     /label=85-036_mgpw_3SIN-ref
                     /note="color: black; sequence: aagtggctaagatctacagc; added:
                     2021-04-13"
     LTR             4124..4357
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     4124..4143
                     /label=90-051_LTR NL4_3 FW1
                     /note="color: black; sequence: TGGAAGGGCTAATTCACTCC; added:
                     2021-04-13"
     primer_bind     4206..4227
                     /label=95-022_Nuc 1 fw (mousseau)
                     /note="color: black; sequence: CTGGGAGCTCTCTGGCTAACTA; 
                     added: 2021-04-13"
     primer_bind     4220..4243
                     /label=83-027_Q-Alu nF out
                     /note="color: black; sequence: GCTAACTAGGGAACCCACTGCTTA; 
                     added: 2021-04-13"
     primer_bind     4220..4243
                     /label=87-092_Q-Alu nF out
                     /note="color: black; sequence: GCTAACTAGGGAACCCACTGCTTA; 
                     added: 2021-04-13"
     primer_bind     4240..4267
                     /label=70-083_454_MKL-3
                     /note="color: black; sequence: 
                     cttaagcctcaataaagcttgccttgag; added: 2021-04-13"
     primer_bind     4240..4267
                     /label=91-059_NGS LV pcr1 hiv ltr
                     /note="color: black; sequence: 
                     cttaagcctcaataaagcttgccttgag; added: 2021-04-13"
     primer_bind     4255..4275
                     /label=82-023_Q-Alu-F-in
                     /note="color: black; sequence: AGCTTGCCTTGAGTGCTTCAA; 
                     added: 2021-04-13"
     primer_bind     4255..4275
                     /label=83-028_Q-Alu-F-in
                     /note="color: black; sequence: AGCTTGCCTTGAGTGCTTCAA; 
                     added: 2021-04-13"
     primer_bind     4255..4275
                     /label=86-047_Q-Alu-F-in
                     /note="color: black; sequence: AGCTTGCCTTGAGTGCTTCAA; 
                     added: 2021-04-13"
     primer_bind     4255..4275
                     /label=87-093_Q-Alu-F-in
                     /note="color: black; sequence: AGCTTGCCTTGAGTGCTTCAA; 
                     added: 2021-04-13"
     primer_bind     complement(4288..4310)
                     /label=95-023_Nuc 1 rv (mousseau
                     /note="color: black; sequence: TTACCAGAGTCACACAACAGACG; 
                     added: 2021-04-13"
     primer_bind     4292..4319
                     /label=70-081_SK_Forward
                     /note="color: black; sequence: 
                     tgttgtgtgactctggtaactagagatc; added: 2021-04-13"
     primer_bind     4305..4326
                     /label=84-087_NGS_QC-LV_s2
                     /note="color: black; sequence: TGGTAACTAGAGATCCCTCAGA; 
                     added: 2021-04-13"
     primer_bind     4312..4343
                     /label=91-063_NGS LV R2
                     /note="color: black; sequence: 
                     tagagatccctcagacccttttagtcagtgtg; added: 2021-04-13"
     primer_bind     complement(4312..4343)
                     /label=91-064_NGS LV index primer
                     /note="color: black; sequence: 
                     cacactgactaaaagggtctgagggatctcta; added: 2021-04-13"
     primer_bind     complement(4315..4338)
                     /label=82-024_Q-Aul-R-in
                     /note="color: black; sequence: TGACTAAAAGGGTCTGAGGGATCT; 
                     added: 2021-04-13"
     primer_bind     complement(4315..4338)
                     /label=83-029_Q-Alu-R-in
                     /note="color: black; sequence: TGACTAAAAGGGTCTGAGGGATCT; 
                     added: 2021-04-13"
     primer_bind     complement(4315..4338)
                     /label=86-048_Q-Alu-R-in
                     /note="color: black; sequence: TGACTAAAAGGGTCTGAGGGATCT; 
                     added: 2021-04-13"
     primer_bind     complement(4315..4338)
                     /label=87-094_Q-Alu-R-in
                     /note="color: black; sequence: TGACTAAAAGGGTCTGAGGGATCT; 
                     added: 2021-04-13"
     primer_bind     complement(4322..4345)
                     /label=88-004_nuc 1 rev
                     /note="color: black; sequence: TCCACACTGACTAAAAGGGTCTGA; 
                     added: 2021-04-13"
     primer_bind     complement(4335..4357)
                     /label=90-053_LTR NL4_3 rev
                     /note="color: black; sequence: TGCTAGAGATTTTCCACACTGAC; 
                     added: 2021-04-13"
     primer_bind     4335..4356
                     /label=91-029_ngs QC LV s3
                     /note="color: black; sequence: gtcagtgtggaaaatctctagc; 
                     added: 2021-04-13"
     primer_bind     4337..4355
                     /label=86-070_seq_CA_s
                     /note="color: black; sequence: cagtgtggaaaatctctag; added: 
                     2021-04-13"
     primer_bind     4342..4358
                     /label=95-067_HSIV fw
                     /note="color: black; sequence: TGGAAAATCTCTAGCAGTGGC; 
                     added: 2021-04-13"
     primer_bind     complement(4381..4400)
                     /label=pcDNA3.1-R
                     /note="color: black; sequence: TAGAAGGCACAGTCGAGGCT; added:
                     2021-04-13"
     primer_bind     complement(4382..4404)
                     /label=100-053_FerrH 1as
                     /note="color: black; sequence: caactagaaggcacagtcgaggc; 
                     added: 2021-04-13"
     primer_bind     complement(4383..4400)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
                     /note="color: black; sequence: TAGAAGGCACAGTCGAGG"
     polyA_signal    4389..4613
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     4397..4419
                     /label=86-051_BGH QPCR S
                     /note="color: black; sequence: TCTAGTTGCCAGCCATCTGTTGT; 
                     added: 2021-04-13"
     primer_bind     4397..4419
                     /label=98-062_BGH S
                     /note="color: black; sequence: TCTAGTTGCCAGCCATCTGTTGT; 
                     added: 2021-04-13"
     primer_bind     complement(4405..4424)
                     /label=77-084_MLV_IN_end_as
                     /note="color: black; sequence: GGCAAACAACAGATGGCTGG; added:
                     2021-04-13"
     primer_bind     complement(4418..4438)
                     /label=93-073_BGHpA_as
                     /note="color: black; sequence: AGGCACGGGGGAGGGGCAAAC; 
                     added: 2021-04-13"
     primer_bind     complement(4418..4437)
                     /label=UniV5his-R
                     /note="color: black; sequence: GGCACGGGGGAGGGGCAAAC; added:
                     2021-04-13"
     primer_bind     complement(4449..4466)
                     /label=86-052_BGH QPCR AS
                     /note="color: black; sequence: TGGGAGTGGCACCTTCCA; added: 
                     2021-04-13"
     primer_bind     complement(4449..4466)
                     /label=98-063_BGH AS
                     /note="color: black; sequence: TGGGAGTGGCACCTTCCA; added: 
                     2021-04-13"
     primer_bind     complement(4500..4523)
                     /label=75-064_pcDNA_R_seq
                     /note="color: black; sequence: AATGACACCTACTCAGACAATGCG; 
                     added: 2021-04-13"
     primer_bind     complement(4512..4531)
                     /label=F1-oriR
                     /note="color: black; sequence: CAGAATAGAATGACACCTAC; added:
                     2021-04-13"
     rep_origin      4659..5087
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(4746..4765)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="color: black; sequence: AGGGAAGAAAGCGAAAGGAG"
     primer_bind     4956..4977
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="color: black; sequence: GTGGACTCTTGTTCCAAACTGG"
     primer_bind     complement(4959..4978)
                     /label=99-097_MeCP2-pc1208_MeCP2-T158M-pc1265_5
                     /note="color: black; sequence: TCCAGTTTGGAACAAGAGTC; added:
                     2021-04-13"
     primer_bind     4980..4999
                     /label=84-016_LEDGF as1
                     /note="color: black; sequence: Caacactcaaccctatctcg; added:
                     2021-04-13"
     primer_bind     4980..4999
                     /label=84-035_MLV-BB_s1
                     /note="color: black; sequence: Caacactcaaccctatctcg; added:
                     2021-04-13"
     primer_bind     5023..5046
                     /label=81-062_pAC-V16-s1
                     /note="color: black; sequence: gattttgccgatttcggcctattg; 
                     added: 2021-04-13"
     primer_bind     complement(5025..5044)
                     /label=100-006_pMeCP2G pc1121_pMeCP2mR-pc1083_5
                     /note="color: black; sequence: ATAGGCCGAAATCGGCAAAA; added:
                     2021-04-13"
     primer_bind     complement(5096..5116)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
                     /note="color: black; sequence: ACCCTAACTGACACACATTCC"
     promoter        5101..5430
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5281..5416
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     5343..5362
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     primer_bind     5345..5363
                     /label=100-007_pMeCP2G pc1121_pMeCP2mR-pc1083_6
                     /note="color: black; sequence: TTTATGCAGAGGCCGAGGC; added: 
                     2021-04-13"
     primer_bind     complement(5365..5377)
                     /label=100-007_pMeCP2G pc1121_pMeCP2mR-pc1083_6
                     /note="color: black; sequence: TTTATGCAGAGGCCGAGGC; added: 
                     2021-04-13"
     primer_bind     5377..5398
                     /label=92-054_Gpt-s
                     /note="color: black; sequence: gagctattccagaagtagtgag; 
                     added: 2021-04-13"
     primer_bind     5386..5403
                     /label=SV40-Do
                     /note="color: black; sequence: CAGAAGTAGTGAGGAGGC; added: 
                     2021-04-13"
     promoter        5478..5525
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5544..5918
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     5883..5901
                     /label=67-062_Zeo_FW
                     /note="color: black; sequence: aactgcgtgcacttcgtgg; added: 
                     2021-04-13"
     polyA_signal    6048..6169
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6053..6075)
                     /label=EGFP-C1-R
                     /note="color: black; sequence: AACCATTATAAGCTGCAATAAAC; 
                     added: 2021-04-13"
     primer_bind     complement(6085..6104)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC"
     primer_bind     6123..6141
                     /label=87-076_pCEPseq_as
                     /note="color: black; sequence: tcactgcattctagttgtg; added: 
                     2021-04-13"
     primer_bind     6124..6144
                     /label=SV40
                     /note="color: black; sequence: CACTGCATTCTAGTTGTGGTT; 
                     added: 2021-04-13"
     primer_bind     complement(6128..6149)
                     /label=92-071_SV40-ori-as
                     /note="color: black; sequence: ggacaaaccacaactagaatgc; 
                     added: 2021-04-13"
     primer_bind     6139..6158
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     primer_bind     6146..6166
                     /label=84-047_MgpW_BB_s3
                     /note="color: black; sequence: Gtccaaactcatcaatgtatc; 
                     added: 2021-04-13"
     primer_bind     complement(6146..6166)
                     /label=84-050_MgpW_BB_as2
                     /note="color: black; sequence: Gatacattgatgagtttggac; 
                     added: 2021-04-13"
     primer_bind     complement(6153..6169)
                     /label=93-017_SV40 pA as
                     /note="color: black; sequence: ttacgccttaagatacattgatgag; 
                     added: 2021-04-13"
     primer_bind     complement(6155..6174)
                     /label=79-089_seqAC1_V16_s9
                     /note="color: black; sequence: catgataagatacattgatg; added:
                     2021-04-13"
     primer_bind     6155..6174
                     /label=79-099_seqAC1_V16_as9
                     /note="color: black; sequence: catcaatgtatcttatcatg; added:
                     2021-04-13"
     primer_bind     complement(6206..6229)
                     /label=M13-24R-BLUE
                     /note="color: black; sequence: AACAGCTATGACCATGATTACGCC; 
                     added: 2021-04-13"
     primer_bind     complement(6214..6232)
                     /label=BluescriptR
                     /note="color: black; sequence: GGAAACAGCTATGACCATG; added: 
                     2021-04-13"
     primer_bind     complement(6217..6234)
                     /label=M13-29R
                     /note="color: black; sequence: CAGGAAACAGCTATGACC; added: 
                     2021-04-13"
     primer_bind     complement(6218..6234)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(6218..6234)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     complement(6231..6253)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     primer_bind     complement(6231..6251)
                     /label=M13-24R
                     /note="color: black; sequence: CGGATAACAATTTCACACAGG; 
                     added: 2021-04-13"
     primer_bind     complement(6232..6251)
                     /label=pQE-F
                     /note="color: black; sequence: CGGATAACAATTTCACACAG; added:
                     2021-04-13"
     protein_bind    6242..6258
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(6266..6296)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6266 .. 6272 / #ffffff / -10
                        2: 6273 .. 6290 / #ffffff
                        3: 6291 .. 6296 / #ffffff / -35"
     primer_bind     complement(6279..6302)
                     /label=MKSF
                     /note="color: black; sequence: CCAGGCTTTACACTTTATGCTTCC; 
                     added: 2021-04-13"
     primer_bind     complement(6307..6326)
                     /label=M13rev2
                     /note="color: black; sequence: GAGTTAGCTCACTCATTAGG; added:
                     2021-04-13"
     protein_bind    6311..6332
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(6336..6353)
                     /label=84-038_MLV-BB_as2
                     /note="color: black; sequence: Gggcagtgagcgcaacgc; added: 
                     2021-04-13"
     primer_bind     6348..6365
                     /label=94-001_pUC19 _Fw
                     /note="color: black; sequence: CTGCCCGCTTTCCAGTCG; added: 
                     2021-04-13"
     primer_bind     complement(6386..6405)
                     /label=79-100_seqAC1_V16_as10
                     /note="color: black; sequence: gttggccgattcattaatgc; added:
                     2021-04-13"
     primer_bind     complement(6430..6447)
                     /label=94-002_pUC19_Rv_100bp
                     /note="color: black; sequence: AAGCGGAAGAGCGCCCAA; added: 
                     2021-04-13"
     primer_bind     complement(6449..6466)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     primer_bind     complement(6494..6514)
                     /label=84-045_MgpW_BB_s1
                     /note="color: black; sequence: Gcctttgagtgagctgatacc; 
                     added: 2021-04-13"
     primer_bind     complement(6506..6529)
                     /label=81-063_pAC-V16-as1
                     /note="color: black; sequence: gataaccgtattaccgcctttgag; 
                     added: 2021-04-13"
     primer_bind     complement(6532..6552)
                     /label=66-074_MVG131867preCMV2_seq
                     /note="color: black; sequence: CTGCGTTATCCCCTGATTCTG; 
                     added: 2021-04-13"
     primer_bind     complement(6532..6552)
                     /label=66-075_MVG131868Gagpol_5_mid
                     /note="color: black; sequence: CTGCGTTATCCCCTGATTCTG; 
                     added: 2021-04-13"
     primer_bind     complement(6549..6568)
                     /label=85-038_mgpw2-reverse
                     /note="color: black; sequence: gctcacatgttctttcctgc; added:
                     2021-04-13"
     primer_bind     complement(6553..6572)
                     /label=74-042_MLVseq_rev4
                     /note="color: black; sequence: TTTTGCTCACATGTTCTTTC; added:
                     2021-04-13"
     primer_bind     complement(6576..6597)
                     /label=94-003_pUC19_Rv_250bp
                     /note="color: black; sequence: TACGGTTCCTGGCCTTTTGCTG; 
                     added: 2021-04-13"
     primer_bind     complement(6586..6606)
                     /label=76-060_Fw_BamHI_gRNA
                     /note="color: black; sequence: 
                     AAAAAAGGATCCGGCCTTTTTACGGTTCCTGG; added: 2021-04-13"
     primer_bind     complement(6587..6606)
                     /label=ASK-FN2
                     /note="color: black; sequence: CGGCCTTTTTACGGTTCCTG; added:
                     2021-04-13"
     rep_origin      complement(6620..7208)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(6651..6670)
                     /label=78-018_p450_seq_origin
                     /note="color: black; sequence: cttgagcgtcgatttttgtg; added:
                     2021-04-13"
     primer_bind     complement(6700..6719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     primer_bind     complement(6700..6718)
                     /label=101-008_pCHMWS-flag_fw5
                     /note="color: black; sequence: ggaaacgcctggtatcttt; added: 
                     2021-04-13"
     primer_bind     6701..6720
                     /label=90-074_MSCV 21
                     /note="color: black; sequence: AAGATACCAGGCGTTTCCCC; added:
                     2021-04-13"
     primer_bind     6803..6823
                     /label=99-006_pMT_seq_fw4-GFP
                     /note="color: black; sequence: GGCGCTTTCTCATAGCTCACG; 
                     added: 2021-04-13"
     primer_bind     complement(6820..6841)
                     /label=100-010_pDest_GST-huMeCP2_fw3
                     /note="color: black; sequence: GAACTGAGATACCTACAGCGTG; 
                     added: 2021-04-13"
     primer_bind     complement(6822..6847)
                     /label=94-004_pUC19_Rv_500bp
                     /note="color: black; sequence: TACACCGAACTGAGATACCTACAGCG; 
                     added: 2021-04-13"
     primer_bind     6861..6882
                     /label=74-029_MLVseq_fwd3
                     /note="color: black; sequence: AAGCTGGGCTGTGTGCACGAAC; 
                     added: 2021-04-13"
     primer_bind     complement(6954..6974)
                     /label=80-023_NGS_MLV_as
                     /note="color: black; sequence: GGCTGCTGCCAGTGGCGATAA; 
                     added: 2021-04-13"
     primer_bind     6972..6991
                     /label=84-039_MLV-BB_s3
                     /note="color: black; sequence: Gccactggtaacaggattag; added:
                     2021-04-13"
     primer_bind     complement(7021..7040)
                     /label=80-032_SIV PCR1 rev
                     /note="color: black; sequence: AGGCCACCACTTCAAGAACT; added:
                     2021-04-13"
     primer_bind     complement(7035..7054)
                     /label=99-100_MeCP2-pc1208_MeCP2-T158M-pc1265_8
                     /note="color: black; sequence: GTGTAGCCGTAGTTAGGCCA; added:
                     2021-04-13"
     primer_bind     complement(7117..7136)
                     /label=81-048_pSRS11_LTR_RV
                     /note="color: black; sequence: tttgccggatcaagagctac; added:
                     2021-04-13"
     primer_bind     complement(7132..7150)
                     /label=100-009_pMeCP2G pc1121_pMeCP2mR-pc1083_8
                     /note="color: black; sequence: CCAGCGGTGGTTTGTTTGC; added: 
                     2021-04-13"
     primer_bind     complement(7181..7204)
                     /label=101-001_pGEX-2TK-empty_fw4
                     /note="color: black; sequence: gatcctttttttctgcgcgtaatc; 
                     added: 2021-04-13"
     primer_bind     7189..7213
                     /label=90-075_MSCV 22
                     /note="color: black; sequence: GCAGAAAAAAAGGATCTCAAGAAGA; 
                     added: 2021-04-13"
     primer_bind     7271..7291
                     /label=85-031_psrs11_Amp_s
                     /note="color: black; sequence: gattttggtcatgagattatc; 
                     added: 2021-04-13"
     primer_bind     complement(7366..7394)
                     /label=94-005_pUC19_Rv_1000bp
                     /note="color: black; sequence: 
                     GATTAAGCATTGGTAACTGTCAGACCAAG; added: 2021-04-13"
     primer_bind     7369..7388
                     /label=84-006_psrs s1
                     /note="color: black; sequence: ggtctgacagttaccaatgc; added:
                     2021-04-13"
     primer_bind     7369..7388
                     /label=84-044_AmpR_as
                     /note="color: black; sequence: ggtctgacagttaccaatgc; added:
                     2021-04-13"
     primer_bind     7376..7396
                     /label=84-010_LEDGF s1
                     /note="color: black; sequence: Cagttaccaatgcttaatcag; 
                     added: 2021-04-13"
     CDS             complement(7379..8239)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7379 .. 8170 / #ccffcc
                        2: 8171 .. 8239 / #ccffcc / signal sequence
                     Cleavage site after base 8170"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7385..7404
                     /label=105-092_GBE_primer2_Amp
                     /note="color: black; sequence: Atgcttaatcagtgaggcac; added:
                     2021-04-13"
     primer_bind     7391..7410
                     /label=85-032_psrs11_Amp2_s
                     /note="color: black; sequence: aatcagtgaggcacctatct; added:
                     2021-04-13"
     primer_bind     complement(7404..7423)
                     /label=74-041_MLVseq_rev3
                     /note="color: black; sequence: AGACAGATCGCTGAGATAGG; added:
                     2021-04-13"
     primer_bind     complement(7437..7458)
                     /label=78-008_p450_seq_ampR_end_rev
                     /note="color: black; sequence: CGACGGGGAGTCAGGCAACTAT; 
                     added: 2021-04-13"
     primer_bind     7530..7554
                     /label=99-089_pMT_seq_fw4_2fw-GFP
                     /note="color: black; sequence: TCACCGGCTCCAGATTTATCAGCAA; 
                     added: 2021-04-13"
     primer_bind     complement(7531..7552)
                     /label=101-007_pCHMWS-flag_fw4
                     /note="color: black; sequence: gctgataaatctggagccggtg; 
                     added: 2021-04-13"
     primer_bind     7683..7703
                     /label=100-059_AmpR Rv
                     /note="color: black; sequence: GTTGCCATTGCTACAGGCATC; 
                     added: 2021-04-13"
     primer_bind     7704..7723
                     /label=90-076_MSCV 23
                     /note="color: black; sequence: GTGGTGTCACGCTCGTCGTT; added:
                     2021-04-13"
     primer_bind     7719..7738
                     /label=74-028_MLVseq_fwd2
                     /note="color: black; sequence: TCGTTTGGTATGGCTTCATT; added:
                     2021-04-13"
     primer_bind     complement(7750..7768)
                     /label=100-058_AmpR Fw
                     /note="color: black; sequence: ACTCGCCTTGATCGTTGGG; added: 
                     2021-04-13"
     primer_bind     7752..7771
                     /label=84-007_psrs s2
                     /note="color: black; sequence: caacgatcaaggcgagttac; added:
                     2021-04-13"
     primer_bind     complement(7799..7818)
                     /label=84-009_psrs as2
                     /note="color: black; sequence: gaggaccgaaggagctaacc; added:
                     2021-04-13"
     primer_bind     complement(7820..7841)
                     /label=84-017_LEDGF as2
                     /note="color: black; sequence: Caacttacttctgacaacgatc; 
                     added: 2021-04-13"
     primer_bind     7887..7907
                     /label=99-021_pMT_seq_fw5-GFP
                     /note="color: black; sequence: CTTACTGTCATGCCATCCGTA; 
                     added: 2021-04-13"
     primer_bind     complement(7896..7920)
                     /label=99-057_huMeCP2 fw2
                     /note="color: black; sequence: CAGAAAAGCATCTTACGGATGGCAT; 
                     added: 2021-04-13"
     primer_bind     7949..7971
                     /label=84-040_MLV-BB_s4
                     /note="color: black; sequence: ctgagaatagtgtatgcggcgac; 
                     added: 2021-04-13"
     primer_bind     complement(7949..7971)
                     /label=84-041_MLV-BB_as3
                     /note="color: black; sequence: Gtcgccgcatacactattctcag; 
                     added: 2021-04-13"
     primer_bind     complement(7967..7986)
                     /label=84-008_psrs as1
                     /note="color: black; sequence: ggcaagagcaactcggtcgc; added:
                     2021-04-13"
     primer_bind     complement(7991..8010)
                     /label=85-034_psrs11_Amp2_rev
                     /note="color: black; sequence: cggtattatcccgtattgac; added:
                     2021-04-13"
     primer_bind     8002..8021
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     primer_bind     complement(8070..8088)
                     /label=84-043_AmpR_s
                     /note="color: black; sequence: Gcggtaagatccttgagag; added: 
                     2021-04-13"
     primer_bind     complement(8071..8090)
                     /label=85-033_psrs11_Amp_rev
                     /note="color: black; sequence: cagcggtaagatccttgaga; added:
                     2021-04-13"
     primer_bind     complement(8074..8094)
                     /label=90-087_HIV2RODsqBBrv1_AB
                     /note="color: black; sequence: TCAACAGCGGTAAGATCCTTG; 
                     added: 2021-04-13"
     primer_bind     8088..8108
                     /label=84-011_LEDGF s2
                     /note="color: black; sequence: Ctgttgagatccagttcgatg; 
                     added: 2021-04-13"
     primer_bind     8154..8175
                     /label=78-007_p450_seq_ampR_start_fwd
                     /note="color: black; sequence: ACCAGCGTTTCTGGGTGAGCAA; 
                     added: 2021-04-13"
     primer_bind     8205..8224
                     /label=90-077_MSCV 24
                     /note="color: black; sequence: GGAATAAGGGCGACACGGAA; added:
                     2021-04-13"
     promoter        complement(8240..8344)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(8259..8278)
                     /label=74-040_MLVseq_rev2
                     /note="color: black; sequence: CCCTGATAAATGCTTCAATA; added:
                     2021-04-13"
     primer_bind     8328..8347
                     /label=sCOS1F
                     /note="color: black; sequence: CAAATAGGGGTTCCGCGCAC; added:
                     2021-04-13"
     primer_bind     complement(8331..8348)
                     /label=94-006_pUC19_Rv_2000bp
                     /note="color: black; sequence: TGTGCGCGGAACCCCTAT; added: 
                     2021-04-13"
     primer_bind     complement(8338..8359)
                     /label=101-006_pCHMWS-flag_fw3
                     /note="color: black; sequence: tttcggggaaatgtgcgcggaa; 
                     added: 2021-04-13"
     primer_bind     complement(8406..8425)
                     /label=84-042_MLV_BB_as4
                     /note="color: black; sequence: gattgtactgagagtgcacc; added:
                     2021-04-13"
     primer_bind     complement(8419..8438)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /note="color: black; sequence: CGGCATCAGAGCAGATTGTA"
     enhancer        8610..8989
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     primer_bind     8626..8652
                     /label=89-042_CMVenh PCR S
                     /note="color: black; sequence: 
                     atttaacttaagtagttattaatagtaatcaattacggg; added: 2021-04-13"
     primer_bind     8666..8685
                     /label=100-001_pMeCP2G pc1121_pMeCP2mR-pc1083_1
                     /note="color: black; sequence: AGCCCATATATGGAGTTCCG; added:
                     2021-04-13"
     primer_bind     8667..8710
                     /label=99-036_CAG S3
                     /note="color: black; sequence: 
                     GCCCATATATGGGCTAGCGCGTTACATAACTTACGGTAAATGGC; added: 
                     2021-04-13"
     primer_bind     8676..8695
                     /label=99-093_MeCP2-pc1208_MeCP2-T158M-pc1265_1
                     /note="color: black; sequence: TGGAGTTCCGCGTTACATAA; added:
                     2021-04-13"
     primer_bind     8684..8710
                     /label=98-037_CAG S2
                     /note="color: black; sequence: 
                     AAAACTTAAGCTAGCGCGTTACATAACTTACGGTAAATGGC; added: 
                     2021-04-13"
     primer_bind     8684..8706
                     /label=98-007_CAG S1
                     /note="color: black; sequence: 
                     CTTAAGCTAGCGCGTTACATAACTTACGGTAAA; added: 2021-04-13"
     primer_bind     8698..8714
                     /label=103-077_CMV_s
                     /note="color: black; sequence: TACGGTAAATGGCCCGC; added: 
                     2021-04-13"
     primer_bind     8702..8719
                     /label=A-048_40-042 MVG1090CMVenh_s
                     /note="color: black; sequence: GTAAATGGCCCGCCTGGCATTAT; 
                     added: 2021-04-13"
     primer_bind     8876..8895
                     /label=84-012_LEDGF s3
                     /note="color: black; sequence: Cctattgacgtcaatgacgg; added:
                     2021-04-13"
     primer_bind     8892..8907
                     /label=103-077_CMV_s
                     /note="color: black; sequence: TACGGTAAATGGCCCGC; added: 
                     2021-04-13"
     primer_bind     8892..8903
                     /label=99-036_CAG S3
                     /note="color: black; sequence: 
                     GCCCATATATGGGCTAGCGCGTTACATAACTTACGGTAAATGGC; added: 
                     2021-04-13"
     primer_bind     8892..8903
                     /label=98-037_CAG S2
                     /note="color: black; sequence: 
                     AAAACTTAAGCTAGCGCGTTACATAACTTACGGTAAATGGC; added: 
                     2021-04-13"
     primer_bind     8895..8917
                     /label=A-048_40-042 MVG1090CMVenh_s
                     /note="color: black; sequence: GTAAATGGCCCGCCTGGCATTAT; 
                     added: 2021-04-13"
     primer_bind     8921..8940
                     /label=105-093_GBE_primer3_CMVenhancer
                     /note="color: black; sequence: Cagtacatgaccttatggga; added:
                     2021-04-13"
     primer_bind     complement(8946..8970)
                     /label=A-049_40-043 MVG1091CMVenh_as
                     /note="color: black; sequence: AATACGTAGATGTACTGCCAAGTAG; 
                     added: 2021-04-13"
     primer_bind     complement(8969..8990)
                     /label=89-043_CMVenh PCR AS
                     /note="color: black; sequence: 
                     atttaaaagcttgtgccatggtaatagcgatgactaa; added: 2021-04-13"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtacct cgagcggccc aagcttaaaa aaattctagt tggtttaacg cgtaactaga
     2041 tagaaccgcg tcactccact gttcataggg atccaagttt ttgcaccgac tcggtgccac
     2101 tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaacta
     2161 tgaacagtgg agtgatcacg gtgtttcgtc ctttccacaa gatatataaa gccaagaaat
     2221 cgaaatactt tcaagttacg gtaagcatat gatagtccat tttaaaacat aattttaaaa
     2281 ctgcaaacta cccaagaaat tattactttc tacgtcacgt attttgtact aatatctttg
     2341 tgtttacagt caaattaatt ctaattatct ctctaacagc cttgtatcgt atatgcaaat
     2401 atgaaggaat catgggaaat aggccctctt cctgcccgac cttggtaccg gatccagtcg
     2461 actgaattgg ttcctttaaa gcctgctttt ttgtacagct agctaggtct tgaaaggagt
     2521 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     2581 gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg gggtaaactg
     2641 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     2701 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagga
     2761 ccggttctag gccaccatgg ctcgaggatc cggcgcaaca aacttctctc tgctgaaaca
     2821 agccggagat gtcgaagaga atcctggacc gaccgagtac aagcccacgg tgcgcctcgc
     2881 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     2941 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     3001 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     3061 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     3121 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     3181 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggagtctc
     3241 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     3301 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     3361 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     3421 gtgcatgacc cgcaagcccg gtgcctgaac gcgttaagtc gacaatcaac ctctggatta
     3481 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3541 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3601 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     3661 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     3721 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     3781 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3841 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     3901 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3961 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     4021 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     4081 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     4141 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     4201 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     4261 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     4321 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
     4381 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4441 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4501 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     4561 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     4621 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     4681 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4741 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4801 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4861 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4921 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4981 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     5041 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     5101 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5161 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5221 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5281 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5341 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     5401 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     5461 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     5521 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     5581 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     5641 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     5701 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     5761 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     5821 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     5881 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     5941 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     6001 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     6061 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6121 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     6181 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6241 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6301 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6361 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6421 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6481 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6541 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6601 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6661 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6721 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6781 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6841 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6901 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6961 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7021 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7081 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7141 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7201 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7261 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7321 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7381 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7441 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7501 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7561 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7621 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7681 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7741 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7801 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7861 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7921 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7981 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8041 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8101 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8161 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8221 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8281 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8341 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     8401 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     8461 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     8521 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     8581 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     8641 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     8701 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     8761 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     8821 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     8881 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     8941 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
//
