LOCUS       Exported                4859 bp ds-DNA     circular SYN 08-AUG-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Aug 8, 2023 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        201..1374
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2247..2273
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             2274..2294
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             2310..2852
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /note="color: #993366"
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     misc_feature    2875..2986
                     /label=bGH poly(A) signal
                     /note="color: #a6acb3"
     misc_feature    3144..3173
                     /label=T3te terminator
                     /note="color: #a6acb3"
     misc_feature    3195..3782
                     /label=ori
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    3794..3821
                     /label=T7Te terminator
                     /note="color: #ffffff"
     CDS             complement(3845..4660)
                     /codon_start=1
                     /label=KanR
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    complement(4661..4763)
                     /label=cat promoter
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    complement(4764..4795)
                     /label=tonB terminator
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
       61 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
      121 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
      181 gttttgcgct gcttcgcgga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
      241 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
      301 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
      361 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
      421 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
      481 gcccttgcgt gccttgaatt acttccacct ggctccagta cgtgattctt gatcccgagc
      541 tggagccagg ggcgggcctt gcgctttagg agccccttcg cctcgtgctt gagttgaggc
      601 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
      661 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
      721 ctggcaagat agtcttgtaa atgcgggcca ggatctgcac actggtattt cggtttttgg
      781 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
      841 gcgagcgcgg ccaccgagaa tcggacgggg gtcggacggg ggtagtctca agctggccgg
      901 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
      961 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctccaggg
     1021 ggctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     1081 aaaggggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     1141 tccaggcacc tcgattagtt ctggagcttt tggagtacgt cgtctttagg ttggggggag
     1201 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     1261 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     1321 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaacaccg
     1381 ccaccatgac catcgagaag cgctacgact tcgtgttcct gttcgacgtg caagacggca
     1441 accccaacgg cgatcctgat gccggaaacc tgcctagaat cgaccctcag acaggcgagg
     1501 gcctcgtgac agatgtgtgc ctgaagcgga aagtgcggaa cttcatccag atgacccaga
     1561 acgacgagca ccacgacatc ttcatcagag agaagggcat cctgaacaac ctgatcgacg
     1621 aggcccacga gcaagagaac gtgaagggca aagagaaagg cgagaaaacc gaggccgcca
     1681 gacagtacat gtgcagccgg tactacgaca tcagaacctt cggcgccgtg atgaccaccg
     1741 gcaagaatgc tggacaagtg cggggacctg tgcagctgac cttcagcaga tccatcgatc
     1801 ccatcatgac cctggaacac agcatcacca gaatggccgt gaccaatgag aaggacgcca
     1861 gcgaaaccgg cgacaacaga accatgggca gaaagttcac cgtgccttac ggcctgtacc
     1921 ggtgccacgg ctttatcagc acccacttcg ccaagcagac cggcttcagc gagaacgacc
     1981 tggaactgtt ttggcaggcc ctggtcaaca tgttcgatca cgatcactct gccgccagag
     2041 gccagatgaa tgccagagga ctgtacgtgt tcgagcacag caacaacctg ggagatgccc
     2101 ctgccgacag cctgttcaag agaatccagg tggtcaagaa agacggcgtg gaagtcgtgc
     2161 ggagcttcga cgattacctg gtgtccgtgg acgacaagaa cctggaagag acaaagctgc
     2221 tgcggaagct cggcggctct gtgggctatc cttacgacgt gccagactac gcccccaaga
     2281 aaaagaggaa ggtggcggcc gctggatccc cttcagggca gatcagcaac caggccctgg
     2341 ctctggcccc tagctccgct ccagtgctgg cccagactat ggtgccctct agtgctatgg
     2401 tgcctctggc ccagccacct gctccagccc ctgtgctgac cccaggacca ccccagtcac
     2461 tgagcgctcc agtgcccaag tctacacagg ccggcgaggg gactctgagt gaagctctgc
     2521 tgcacctgca gttcgacgct gatgaggacc tgggagctct gctggggaac agcaccgatc
     2581 ccggagtgtt cacagatctg gcctccgtgg acaactctga gtttcagcag ctgctgaatc
     2641 agggcgtgtc catgtctcat agtacagccg aaccaatgct gatggagtac cccgaagcca
     2701 ttacccggct ggtgaccggc agccagcggc cccccgaccc cgctccaact cccctgggaa
     2761 ccagcggcct gcctaatggg ctgtccggag atgaagactt ctcaagcatc gctgatatgg
     2821 actttagtgc cctgctgtca cagatttcct cttgacgtcg ctgatcagcc tcgactgtgc
     2881 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     2941 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     3001 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     3061 acaatagcag gaacgaattc aagcttgata tcattcagga cgagcctcag actccagcgt
     3121 aactggactg caatcaactc actggctcac cttcacgggt gggcctttct tcggtagaaa
     3181 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3241 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3301 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3361 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3421 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3481 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3541 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3601 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3661 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3721 gccacctctg acttgagcat cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3781 aaaacgccag caacgcagaa aggcccaccc gaaggtgagc caggtgatta catttgggcc
     3841 ctcattagaa aaactcatcg agcatcaagt gaaactgcaa tttattcata tcaggattat
     3901 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     3961 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     4021 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     4081 cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag
     4141 gccagccatt acgctcgtca tcaaaatcac tcgcaccaac caaaccgtta ttcattcgtg
     4201 attgcgcctg agcgagacga aatacgcgat cgccgttaaa aggacaatta caaacaggaa
     4261 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     4321 gatattcttc taatacctgg aatgctgttt tccctgggat cgcagtggtg agtaaccatg
     4381 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     4441 agtttagcct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     4501 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc
     4561 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     4621 gcggcctcga gcaagacgtt tcccgttgaa tatggctcat tttagcttcc ttagctcctg
     4681 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     4741 tggaacctct tacgtgccga tcaagtcaaa agcctccggt cggaggcttt tgactttctg
     4801 ctatggaggt caggtatgat ttaaatggtc agtattgagc gatatctaga gaattcgtc
//
