LOCUS       207411-attachmen       11437 bp DNA     circular SYN 27-MAR-2024
DEFINITION  HO mAIRN 2xLEXA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11437)
  AUTHORS   Hamperl S, Bocek MJ, Saldivar JC, Swigut T, Cimprich KA.
  TITLE     Transcription-Replication Conflict Orientation Modulates R-Loop 
            Levels and Activates Distinct DNA Damage Responses.
  JOURNAL   Cell 2017 Aug;170:774-786.e19
  PUBMED    28802045
REFERENCE   2  (bases 1 to 11437)
  AUTHORS   AG Hamperl
  TITLE     Direct Submission
  JOURNAL   Exported Mar 27, 2024 from SnapGene 6.2.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07.043"; journalName: "Cell"; date: "2017-08"; 
            volume: "170"; pages: "774-786.e19"
FEATURES             Location/Qualifiers
     source          1..11437
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    248..296
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(338..1375)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(1418..1563)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     protein_bind    1745..1760
                     /label=LexA-binding site
                     /bound_moiety="Functionality of site was shown
                     experimentally!Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA10.1101/gad.1355605"
                     /note="Functionality of site was shown experimentally, in
                     our lab!"
                     /note="Although consensus sequence is usually:
                     TACTG(TA)5CAGTA
                     Wade, Joseph T., Nikos B. Reppas, George M. Church, and 
                     Kevin Struhl. ""Genomic Analysis of LexA Binding Reveals 
                     the Permissive Nature of the Escherichia Coli Genome and 
                     Identifies Unconventional Target Sites."" Genes "
     protein_bind    1779..1794
                     /label=LexA-binding site
                     /bound_moiety="Functionality of site was shown
                     experimentally!Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA10.1101/gad.1355605"
                     /note="Functionality of site was shown experimentally, in
                     our lab!"
                     /note="Although consensus sequence is usually:
                     TACTG(TA)5CAGTA
                     Wade, Joseph T., Nikos B. Reppas, George M. Church, and 
                     Kevin Struhl. ""Genomic Analysis of LexA Binding Reveals 
                     the Permissive Nature of the Escherichia Coli Genome and 
                     Identifies Unconventional Target Sites."" Genes "
     protein_bind    1813..1828
                     /label=LexA-binding site
                     /bound_moiety="Functionality of site was shown
                     experimentally!Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA10.1101/gad.1355605"
                     /note="Functionality of site was shown experimentally, in
                     our lab!"
                     /note="Although consensus sequence is usually:
                     TACTG(TA)5CAGTA
                     Wade, Joseph T., Nikos B. Reppas, George M. Church, and 
                     Kevin Struhl. ""Genomic Analysis of LexA Binding Reveals 
                     the Permissive Nature of the Escherichia Coli Genome and 
                     Identifies Unconventional Target Sites."" Genes "
     rep_origin      complement(2119..2707)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2878..3738)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3739..3843)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4364..6289
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     rep_origin      6591..8380
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     polyA_signal    9104..9238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     9196..9216
                     /label=ECFP_rev
                     /note="LIID:0836	ECFP	qPCR	Manuel Trauner"
     promoter        9245..9263
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(9290..10607)
                     /codon_start=1
                     /product="custom"
                     /label=mAIRN_reporter
                     /note="/vntifkey=4"
                     /translation="WGTEVS*GSPREARQCEGRILQMRVGFRCKGRIPRVRGFRKGRVP
                     QEGRALREVRVPLIRQLEGSDQEFQAAQRFPYARFSARVKISGLQENSALGAPLLTSVL
                     NYTRADRAGRKPKAAAAAMPRQQEEKGGGRPARADCCTGGGGGLRAI*STRVPRCARGA
                     GVRARAPRCWRVGGGGGDRRISTRPDPRFAGQGEGSVGSAVELWRSEDFYTT*SAVCGE
                     GGRFGGFRGTARARVLACALPPPPPPRSEDFSTTPIVLRFWLCRVAREAKGEKLHKEGI
                     RRV*RVPRHCAAVLPEGSEQFRLCRDPWRTPAGPCALVVLSCGEGKGRESSEGSELS*G
                     CEAAQGQGSEGSAALREPRCRAAQRFGGF*CDSGWAVILVMPSCARGGEETARRIPRYP
                     EGANCTRGGFEGFCDQGQRSKVPCYRRDELKSATLQERQA"
     primer_bind     complement(9423..9442)
                     /label=mAIRN_2_fwd
                     /note="LIID:0831	mAIRN	qPCR MT049	Manuel Trauner"
     primer_bind     9660..9679
                     /label=ECFP_fwd
                     /note="LIID:0826	ECFP	qPCR MT044	Manuel Trauner"
     primer_bind     complement(9786..9805)
                     /label=mAIRN_rep_fwd
                     /note="LIID:0830	mAIRN	qPCR MT048	Manuel Trauner"
     primer_bind     10271..10290
                     /label=mAIRN1_rev
                     /note="LIID:0829	mAIRN	qPCR MT047	Manuel Trauner"
     primer_bind     complement(10398..10417)
                     /label=mAIRN1_fwd
                     /note="LIID:0828	mAIRN	qPCR MT046	Manuel Trauner"
     promoter        complement(10630..10944)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    10704..10722
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10740..10758
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10775..10793
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10811..10829
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10847..10865
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10882..10900
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10918..10936
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11070..11085
                     /label=LexA-binding site
                     /bound_moiety="Functionality of site was shown
                     experimentally!Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA10.1101/gad.1355605"
                     /note="Functionality of site was shown experimentally, in
                     our lab!"
                     /note="Although consensus sequence is usually:
                     TACTG(TA)5CAGTA
                     Wade, Joseph T., Nikos B. Reppas, George M. Church, and 
                     Kevin Struhl. ""Genomic Analysis of LexA Binding Reveals 
                     the Permissive Nature of the Escherichia Coli Genome and 
                     Identifies Unconventional Target Sites."" Genes "
     protein_bind    11104..11119
                     /label=LexA-binding site
                     /bound_moiety="Functionality of site was shown
                     experimentally!Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA10.1101/gad.1355605"
                     /note="Functionality of site was shown experimentally, in
                     our lab!"
                     /note="Although consensus sequence is usually:
                     TACTG(TA)5CAGTA
                     Wade, Joseph T., Nikos B. Reppas, George M. Church, and 
                     Kevin Struhl. ""Genomic Analysis of LexA Binding Reveals 
                     the Permissive Nature of the Escherichia Coli Genome and 
                     Identifies Unconventional Target Sites."" Genes "
     protein_bind    11138..11153
                     /label=LexA-binding site
                     /bound_moiety="Functionality of site was shown
                     experimentally!Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA10.1101/gad.1355605"
                     /note="Functionality of site was shown experimentally, in
                     our lab!"
                     /note="Although consensus sequence is usually:
                     TACTG(TA)5CAGTA
                     Wade, Joseph T., Nikos B. Reppas, George M. Church, and 
                     Kevin Struhl. ""Genomic Analysis of LexA Binding Reveals 
                     the Permissive Nature of the Escherichia Coli Genome and 
                     Identifies Unconventional Target Sites."" Genes "
ORIGIN
        1 cgcaacgccc aaaataataa cccccacgaa ccataaacca ttccccatgg gggaccccgt
       61 ccctaaccca cggggccagt ggctatggca gggcctgccg ccccgacgtt ggctgcgagc
      121 cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat
      181 tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg
      241 cgtttatgaa caaacgaccc aacacccgtg cgttttattc tgtcttttta ttgccgtcat
      301 agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca gttagcctcc cccatctccc
      361 gatccggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
      421 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
      481 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
      541 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg
      601 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
      661 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
      721 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
      781 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
      841 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
      901 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
      961 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
     1021 tggcctccgc gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg
     1081 tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt
     1141 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt
     1201 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga
     1261 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga
     1321 acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc
     1381 attgcccccc gggatctgcg gcacgctgtt gacgctgtta agcgggtcgc tgcagggtcg
     1441 ctcggtgttc gaggccacac gcgtcacctt aatatgcgaa gtggacctcg gaccgcgccg
     1501 ccccgactgc atctgcgtgt tcgaattcgc caatgacaag acgctgggcg gggtttgtgt
     1561 cataacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttgaagctgt
     1621 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     1681 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cggatctcat
     1741 ggtgctgtat ataaaaccag tggttatatg tacagtagct gtatataaaa ccagtggtta
     1801 tatgtacagt agctgtatat aaaaccagtg gttatatgta cagtagtacg ttagatccga
     1861 acgccagcaa gacgtagccc agcgcgtcgg ccccgagatg cgccgcgtgc ggctgctgga
     1921 gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg
     1981 caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccctgc
     2041 ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg
     2101 catgtcttta gttctatgtt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2161 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2221 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2281 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2341 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2401 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2461 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2521 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2581 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2641 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2701 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2761 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2821 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2881 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2941 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3001 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3061 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3121 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3181 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3241 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3301 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3361 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3421 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3481 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3541 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3601 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3661 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3721 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3781 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3841 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     3901 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattct catgtttgac
     3961 agcttatcat cgataagctg atcctcacag gccgcaccca gcttttcttc cgttgcccca
     4021 gtagcatctc tgtctggtga ccttgaagag gaagaggagg ggtcccgaga atccccatcc
     4081 ctaccgtcca gcaaaaaggg ggacgaggaa tttgaggcct ggcttgaggc tcaggacgca
     4141 aatcttgagg atgttcagcg ggagttttcc gggctgcgag taattggtga tgaggacgag
     4201 gatggttcgg aggatgggga attttcagac ctggatctgt ctgacagcga ccatgaaggg
     4261 gatgagggtg ggggggctgt tggagggggc aggagtctgc actccctgta ttcactgagc
     4321 gtcgtctaat aaagatgtct attgatctct tttagtgtga atcatgtctg acgaggggcc
     4381 aggtacagga cctggaaatg gcctaggaga gaagggagac acatctggac cagaaggctc
     4441 cggcggcagt ggacctcaaa gaagaggggg tgataaccat ggacgaggac ggggaagagg
     4501 acgaggacga ggaggcggaa gaccaggagc cccgggcggc tcaggatcag ggccaagaca
     4561 tagagatggt gtccggagac cccaaaaacg tccaagttgc attggctgca aagggaccca
     4621 cggtggaaca ggagcaggag caggagcggg aggggcagga gcaggagggg caggagcagg
     4681 aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag gggcaggagc
     4741 aggaggaggg gcaggagcag gaggaggggc aggaggggca ggaggggcag gagcaggagg
     4801 aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggagggg caggaggggc
     4861 aggaggggca ggagcaggag gaggggcagg agcaggagga ggggcaggag gggcaggagc
     4921 aggaggaggg gcaggagggg caggaggggc aggagcagga ggaggggcag gagcaggagg
     4981 ggcaggaggg gcaggagggg caggagcagg aggggcagga gcaggaggag gggcaggagg
     5041 ggcaggaggg gcaggagcag gaggggcagg agcaggaggg gcaggagcag gaggggcagg
     5101 agcaggaggg gcaggagggg caggagcagg aggggcagga ggggcaggag caggaggggc
     5161 aggaggggca ggagcaggag gaggggcagg aggggcagga gcaggaggag gggcaggagg
     5221 ggcaggagca ggaggggcag gaggggcagg agcaggaggg gcaggagggg caggagcagg
     5281 aggggcagga ggggcaggag caggaggagg ggcaggagca ggaggggcag gagcaggagg
     5341 tggaggccgg ggtcgaggag gcagtggagg ccggggtcga ggaggtagtg gaggccgggg
     5401 tcgaggaggt agtggaggcc gccggggtag aggacgtgaa agagccaggg ggggaagtcg
     5461 tgaaagagcc agggggagag gtcgtggacg tggagaaaag aggcccagga gtcccagtag
     5521 tcagtcatca tcatccgggt ctccaccgcg caggccccct ccaggtagaa ggccattttt
     5581 ccaccctgta ggggaagccg attattttga ataccaccaa gaaggtggcc cagatggtga
     5641 gcctgacgtg cccccgggag cgatagagca gggccccgca gatgacccag gagaaggccc
     5701 aagcactgga ccccggggtc agggtgatgg aggcaggcgc aaaaaaggag ggtggtttgg
     5761 aaagcatcgt ggtcaaggag gttccaaccc gaaatttgag aacattgcag aaggtttaag
     5821 agctctcctg gctaggagtc acgtagaaag gactaccgac gaaggaactt gggtcgccgg
     5881 tgtgttcgta tatggaggta gtaagacctc cctttacaac ctaaggcgag gaactgccct
     5941 tgctattcca caatgtcgtc ttacaccatt gagtcgtctc ccctttggaa tggcccctgg
     6001 acccggccca caacctggcc cgctaaggga gtccattgtc tgttatttca tggtcttttt
     6061 acaaactcat atatttgctg aggttttgaa ggatgcgatt aaggaccttg ttatgacaaa
     6121 gcccgctcct acctgcaata tcagggtgac tgtgtgcagc tttgacgatg gagtagattt
     6181 gcctccctgg tttccaccta tggtggaagg ggctgccgcg gagggtgatg acggagatga
     6241 cggagatgaa ggaggtgatg gagatgaggg tgaggaaggg caggagtgat gtaacttgtt
     6301 aggagacgcc ctcaatcgta ttaaaagccg tgtattcccc cgcactaaag aataaatccc
     6361 cagtagacat catgcgtgct gttggtgtat ttctggccat ctgtcttgtc accattttcg
     6421 tcctcccaac atggggcaat tgggcatacc catgttgtca cgtcactcag ctccgcgctc
     6481 aacaccttct cgcgttggaa aacattagcg acatttacct ggtgagcaat cagacatgcg
     6541 acggctttag cctggcctcc ttaaattcac ctaagaatgg gagcaaccag caggaaaagg
     6601 acaagcagcg aaaattcacg cccccttggg aggtggcggc atatgcaaag gatagcactc
     6661 ccactctact actgggtatc atatgctgac tgtatatgca tgaggatagc atatgctacc
     6721 cggatacaga ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc
     6781 cagatataga ttaggatagc ctatgctacc cagatataaa ttaggatagc atatactacc
     6841 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc
     6901 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc atatgctatc
     6961 cagatatttg ggtagtatat gctacccaga tataaattag gatagcatat actaccctaa
     7021 tctctattag gatagcatat gctacccgga tacagattag gatagcatat actacccaga
     7081 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga
     7141 tataaattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga
     7201 tatagattag gatagcctat gctacccaga tatagattag gatagcatat gctatccaga
     7261 tatttgggta gtatatgcta cccatggcaa cattagccca ccgtgctctc agcgacctcg
     7321 tgaatatgag gaccaacaac cctgtgcttg gcgctcaggc gcaagtgtgt gtaatttgtc
     7381 ctccagatcg cagcaatcgc gcccctatct tggcccgccc acctacttat gcaggtattc
     7441 cccggggtgc cattagtggt tttgtgggca agtggtttga ccgcagtggt tagcggggtt
     7501 acaatcagcc aagttattac acccttattt tacagtccaa aaccgcaggg cggcgtgtgg
     7561 gggctgacgc gtgcccccac tccacaattt caaaaaaaag agtggccact tgtctttgtt
     7621 tatgggcccc attggcgtgg agccccgttt aattttcggg ggtgttagag acaaccagtg
     7681 gagtccgctg ctgtcggcgt ccactctctt tccccttgtt acaaatagag tgtaacaaca
     7741 tggttcacct gtcttggtcc ctgcctggga cacatcttaa taaccccagt atcatattgc
     7801 actaggatta tgtgttgccc atagccataa attcgtgtga gatggacatc cagtctttac
     7861 ggcttgtccc caccccatgg atttctattg ttaaagatat tcagaatgtt tcattcctac
     7921 actagtattt attgcccaag gggtttgtga gggttatatt ggtgtcatag cacaatgcca
     7981 ccactgaacc ccccgtccaa attttattct gggggcgtca cctgaaacct tgttttcgag
     8041 cacctcacat acaccttact gttcacaact cagcagttat tctattagct aaacgaagga
     8101 gaatgaagaa gcaggcgaag attcaggaga gttcactgcc cgctccttga tcttcagcca
     8161 ctgcccttgt gactaaaatg gttcactacc ctcgtggaat cctgacccca tgtaaataaa
     8221 accgtgacag ctcatggggt gggagatatc gctgttcctt aggacccttt tactaaccct
     8281 aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg gttagtctgg
     8341 atagtatata ctactacccg ggaagcatat gctacccgtt tagggttaac aagggggcct
     8401 tataaacact attgctaatg ccctcttgag ggtccgctta tcggtagcta cacaggcccc
     8461 tctgattgac gttggtgtag cctcccgtag tcttcctggg cccctgggag gtacatgtcc
     8521 cccagcattg gtgtaagagc ttcagccaag agttacacat aaaggcaatg ttgtgttgca
     8581 gtccacagac tgcaaagtct gctccaggat gaaagccact cagtgttggc aaatgtgcac
     8641 atccatttat aaggatgtca actacagtca gagaacccct ttgtgtttgg tccccccccg
     8701 tgtcacatgt ggaacagggc ccagttggca agttgtacca accaactgaa gggattacat
     8761 gcactgcccc gcgaagaagg ggcagagatg tcgtagtcag gtttagttcg tccggggcgg
     8821 ggatcgatcc tctagagtcg acctcatggc tgcgccccga cacccgccaa cacccgctga
     8881 cgcgcctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     8941 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagccggat
     9001 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     9061 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     9121 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9181 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcct
     9241 gcagtaatac gactcactat agggcgaatt ggagctccac atcaagcttg agcttgcctc
     9301 tcttgcaacg tggcactttt gagttcatct ctcctgtaac atggcacttt tgagcgttgg
     9361 ccctgatcac agaacccttc gaatcctccc cttgtgcagt ttgcaccctc aggatacctc
     9421 ggaatcctcc gagccgtctc ttcccctccc ctcgcgcaac ttggcataac cagaatcaca
     9481 gcccaaccgg aatcgcatta aaaccctccg aacctttggg cagcgcggca ccggggctca
     9541 cgcagtgccg cggaaccctc ggaaccctgc ccttgtgcag cttcgcaccc tcaggatagc
     9601 tcggaaccct ctgagctttc ccttcccttt ccctcaccgc aactcagcac aaccaaggca
     9661 catggtcctg ctggagttcg ccaaggatca cggcacaacc ggaattgctc cgaaccctcg
     9721 ggcagcactg cggcgcagtg ccgcggaacc ctctaaaccc tccgaatccc ctccttgtgc
     9781 agcttttcac ccttagcctc tctcgcaact cggcacaacc agaatcgcag cacaattgga
     9841 gttgtgctaa aatcctctga acgggggggg gggggggggg ggcagcgcac acgccagcac
     9901 cctggctcgc gcagtgccgc ggaacccacc gaaccttccc cctgccccgc aaaccgcgga
     9961 tcaggtcgtg tagaaatcct ccgatctcca cccccccccc aactctccag cagcgtggtg
    10021 ccctagcgcg aacccctgct ccccttgcgc agcgtggcac cctcgtgctc tagatcgccc
    10081 gtaaaccccc ccccccccag tgcagcagtc ggcccgcgct ggacgccccc ctcccttctc
    10141 ctcttgctga cgcggcatgg cggcagcgcg gctttcggct ttctaccggc cctatcggcc
    10201 ctcgtgtagt tcagaacact ggtgagcagt ggggcaccct agtgctgagt tttcttgtag
    10261 cccagaaatc ttcaccctag cgctgaatct cgcgtagggg aacctttgag cggcctggaa
    10321 ctcttggtcg gagccctcga gctgccggat cagtggaacc ctcacctcgc gtagagccct
    10381 tccctcctgt ggaacccttc ctttgcggaa tcctctaacg cgtggaatcc tccccttgca
    10441 acggaatcct accctcatct gcagaatcct cccctcgcat tgccgcgctt cacgcgggga
    10501 accctagctt acctcagttc cccaggatgt ctgcgtggta actggcggtg ctgtgcttct
    10561 tgctgcccgc tagagcaagg agggatagat ataatgttga agcctcgggt accgagctcg
    10621 aattctccag gcgatctgac ggttcactaa acgagctctg cttatatagg cctcccaccg
    10681 tacacgccta cctcgacata cgttctctat cactgatagg gagtaaactc gacatacgtt
    10741 ctctatcact gatagggata aactcgacat acgttctcta tcactgatag ggagtaaact
    10801 cgacatacgt tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat
    10861 agggagtaaa ctcgacatcg ttctctatca ctgataggga gtaaactcga catacgttct
    10921 ctatcactga tagggagtaa actcgtgtac atattatgat atagatacaa cgtatgcaat
    10981 ggccaatagc caatattgat ttatgctata taaccaatga ctaatatggc taattgccaa
    11041 tattgattca atgtatagat ctcatggtgc tgtatataaa accagtggtt atatgtacag
    11101 tagctgtata taaaaccagt ggttatatgt acagtagctg tatataaaac cagtggttat
    11161 atgtacagta gtacgttaga tcttccatac ctaccagttc tgcgcctgca gcaatggcaa
    11221 caacgttgcc cgggatctgc gatgataagc tgtcaaacat gagaattggt cgactagctt
    11281 ggcacgccag aaatccgcgc ggtggttttt gggggtcggg ggtgtttggc agccacagac
    11341 gcccggtgtt cgtgtcgcgc cagtacatgc ggtccatgcc caggccatcc aaaaaccatg
    11401 ggtctgtctg ctcagtccag tcgtggacct gacccca
//
