LOCUS       pSH26       10822 bp DNA     circular SYN 06-OCT-2023
DEFINITION  HO ECFP 2xLEXA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10822)
  AUTHORS   Hamperl S, Bocek MJ, Saldivar JC, Swigut T, Cimprich KA.
  TITLE     Transcription-Replication Conflict Orientation Modulates R-Loop 
            Levels and Activates Distinct DNA Damage Responses.
  JOURNAL   Cell 2017 Aug;170:774-786.e19
  PUBMED    28802045
REFERENCE   2  (bases 1 to 10822)
  AUTHORS   AG Hamperl
  TITLE     Direct Submission
  JOURNAL   Exported Jan 31, 2024 from SnapGene 6.2.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07.043"; journalName: "Cell"; date: "2017-08"; 
            volume: "170"; pages: "774-786.e19"
FEATURES             Location/Qualifiers
     source          1..10822
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(169..2094)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     promoter        2615..2719
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2720..3580
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3751..4339
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4521..4536
                     /label=LexA-binding site
                     /bound_moiety="Functionality of site was shown 
                     experimentally!Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA10.1101/gad.1355605"
                     /note="Functionality of site was shown experimentally, in 
                     our lab!"
                     /note="Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA
                     
                     Wade, Joseph T., Nikos B. Reppas, George M. Church, and 
                     Kevin Struhl. "Genomic Analysis of LexA Binding Reveals the
                     Permissive Nature of the Escherichia Coli Genome and 
                     Identifies Unconventional Target Sites." Genes "
     protein_bind    4555..4570
                     /label=LexA-binding site
                     /bound_moiety="Functionality of site was shown 
                     experimentally!Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA10.1101/gad.1355605"
                     /note="Functionality of site was shown experimentally, in 
                     our lab!"
                     /note="Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA
                     
                     Wade, Joseph T., Nikos B. Reppas, George M. Church, and 
                     Kevin Struhl. "Genomic Analysis of LexA Binding Reveals the
                     Permissive Nature of the Escherichia Coli Genome and 
                     Identifies Unconventional Target Sites." Genes "
     protein_bind    4589..4604
                     /label=LexA-binding site
                     /bound_moiety="Functionality of site was shown 
                     experimentally!Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA10.1101/gad.1355605"
                     /note="Functionality of site was shown experimentally, in 
                     our lab!"
                     /note="Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA
                     
                     Wade, Joseph T., Nikos B. Reppas, George M. Church, and 
                     Kevin Struhl. "Genomic Analysis of LexA Binding Reveals the
                     Permissive Nature of the Escherichia Coli Genome and 
                     Identifies Unconventional Target Sites." Genes "
     promoter        4895..5040
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             5083..6120
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     polyA_signal    6162..6210
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    6741..6756
                     /label=LexA-binding site
                     /bound_moiety="Functionality of site was shown 
                     experimentally!Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA10.1101/gad.1355605"
                     /note="Functionality of site was shown experimentally, in 
                     our lab!"
                     /note="Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA
                     
                     Wade, Joseph T., Nikos B. Reppas, George M. Church, and 
                     Kevin Struhl. "Genomic Analysis of LexA Binding Reveals the
                     Permissive Nature of the Escherichia Coli Genome and 
                     Identifies Unconventional Target Sites." Genes "
     protein_bind    6775..6790
                     /label=LexA-binding site
                     /bound_moiety="Functionality of site was shown 
                     experimentally!Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA10.1101/gad.1355605"
                     /note="Functionality of site was shown experimentally, in 
                     our lab!"
                     /note="Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA
                     
                     Wade, Joseph T., Nikos B. Reppas, George M. Church, and 
                     Kevin Struhl. "Genomic Analysis of LexA Binding Reveals the
                     Permissive Nature of the Escherichia Coli Genome and 
                     Identifies Unconventional Target Sites." Genes "
     protein_bind    6809..6824
                     /label=LexA-binding site
                     /bound_moiety="Functionality of site was shown 
                     experimentally!Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA10.1101/gad.1355605"
                     /note="Functionality of site was shown experimentally, in 
                     our lab!"
                     /note="Although consensus sequence is usually: 
                     TACTG(TA)5CAGTA
                     
                     Wade, Joseph T., Nikos B. Reppas, George M. Church, and 
                     Kevin Struhl. "Genomic Analysis of LexA Binding Reveals the
                     Permissive Nature of the Escherichia Coli Genome and 
                     Identifies Unconventional Target Sites." Genes "
     promoter        6950..7264
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    6958..6976
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6994..7012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7029..7047
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7065..7083
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7101..7119
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7136..7154
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7172..7190
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      7288..7297
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7294..8010
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    8043..8177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8900..10689
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 caacatgggt atgcccaatt gccccatgtt gggaggacga aaatggtgac aagacagatg
       61 gccagaaata caccaacagc acgcatgatg tctactgggg atttattctt tagtgcgggg
      121 gaatacacgg cttttaatac gattgagggc gtctcctaac aagttacatc actcctgccc
      181 ttcctcaccc tcatctccat cacctccttc atctccgtca tctccgtcat caccctccgc
      241 ggcagcccct tccaccatag gtggaaacca gggaggcaaa tctactccat cgtcaaagct
      301 gcacacagtc accctgatat tgcaggtagg agcgggcttt gtcataacaa ggtccttaat
      361 cgcatccttc aaaacctcag caaatatatg agtttgtaaa aagaccatga aataacagac
      421 aatggactcc cttagcgggc caggttgtgg gccgggtcca ggggccattc caaaggggag
      481 acgactcaat ggtgtaagac gacattgtgg aatagcaagg gcagttcctc gccttaggtt
      541 gtaaagggag gtcttactac ctccatatac gaacacaccg gcgacccaag ttccttcgtc
      601 ggtagtcctt tctacgtgac tcctagccag gagagctctt aaaccttctg caatgttctc
      661 aaatttcggg ttggaacctc cttgaccacg atgctttcca aaccaccctc cttttttgcg
      721 cctgcctcca tcaccctgac cccggggtcc agtgcttggg ccttctcctg ggtcatctgc
      781 ggggccctgc tctatcgctc ccgggggcac gtcaggctca ccatctgggc caccttcttg
      841 gtggtattca aaataatcgg cttcccctac agggtggaaa aatggccttc tacctggagg
      901 gggcctgcgc ggtggagacc cggatgatga tgactgacta ctgggactcc tgggcctctt
      961 ttctccacgt ccacgacctc tccccctggc tctttcacga cttccccccc tggctctttc
     1021 acgtcctcta ccccggcggc ctccactacc tcctcgaccc cggcctccac tacctcctcg
     1081 accccggcct ccactgcctc ctcgaccccg gcctccacct cctgctcctg cccctcctgc
     1141 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc
     1201 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctcctgctcc
     1261 tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc
     1321 tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg ctcctgcccc
     1381 tcctgctcct gcccctcctg ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc
     1441 tgcccctcct gctcctgccc ctcctgcccc tcctgcccct cctgctcctg cccctcctcc
     1501 tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     1561 tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc
     1621 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc
     1681 ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     1741 tgcccctcct gcccctcctc ctgctcctgc ccctcctcct gctcctgccc ctcctgctcc
     1801 tgcccctccc gctcctgctc ctgctcctgt tccaccgtgg gtccctttgc agccaatgca
     1861 acttggacgt ttttggggtc tccggacacc atctctatgt cttggccctg atcctgagcc
     1921 gcccggggct cctggtcttc cgcctcctcg tcctcgtcct cttccccgtc ctcgtccatg
     1981 gttatcaccc cctcttcttt gaggtccact gccgccggag ccttctggtc cagatgtgtc
     2041 tcccttctct cctaggccat ttccaggtcc tgtacctggc ccctcgtcag acatgattca
     2101 cactaaaaga gatcaataga catctttatt agacgacgct cagtgaatac agggagtgca
     2161 gactcctgcc ccctccaaca gcccccccac cctcatcccc ttcatggtcg ctgtcagaca
     2221 gatccaggtc tgaaaattcc ccatcctccg aaccatcctc gtcctcatca ccaattactc
     2281 gcagcccgga aaactcccgc tgaacatcct caagatttgc gtcctgagcc tcaagccagg
     2341 cctcaaattc ctcgtccccc tttttgctgg acggtaggga tggggattct cgggacccct
     2401 cctcttcctc ttcaaggtca ccagacagag atgctactgg ggcaacggaa gaaaagctgg
     2461 gtgcggcctg tgaggatcag cttatcgatg ataagctgtc aaacatgaga attcttgaag
     2521 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     2581 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2641 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     2701 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     2761 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     2821 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     2881 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     2941 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     3001 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     3061 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     3121 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     3181 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     3241 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     3301 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     3361 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     3421 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     3481 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     3541 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     3601 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     3661 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3721 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3781 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3841 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     3901 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3961 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4021 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4081 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4141 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4201 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4261 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4321 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4381 ctggccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa
     4441 cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc
     4501 tcgcgtcgga tctcatggtg ctgtatataa aaccagtggt tatatgtaca gtagctgtat
     4561 ataaaaccag tggttatatg tacagtagct gtatataaaa ccagtggtta tatgtacagt
     4621 agtacgttag atccgaacgc cagcaagacg tagcccagcg cgtcggcccc gagatgcgcc
     4681 gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg
     4741 cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag
     4801 cgaggtgccg ccctgcttca tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc
     4861 ggaagaaata tatttgcatg tctttagttc tatgatgaca caaaccccgc ccagcgtctt
     4921 gtcattggcg aattcgaaca cgcagatgca gtcggggcgg cgcggtccga ggtccacttc
     4981 gcatattaag gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg acccgcttaa
     5041 cagcgtcaac agcgtgccgc agatcccggg gggcaatgag atatgaaaaa gcctgaactc
     5101 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     5161 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     5221 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     5281 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc
     5341 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     5401 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
     5461 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     5521 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     5581 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     5641 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     5701 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     5761 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     5821 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
     5881 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     5941 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     6001 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     6061 gatagtggaa accgacgccc cagcactcgt ccggatcggg agatggggga ggctaactga
     6121 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     6181 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     6241 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     6301 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     6361 caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca tggggaatgg
     6421 tttatggttc gtgggggtta ttattttggg cgttgcgtgg ggtcaggtcc acgactggac
     6481 tgagcagaca gacccatggt ttttggatgg cctgggcatg gaccgcatgt actggcgcga
     6541 cacgaacacc gggcgtctgt ggctgccaaa cacccccgac ccccaaaaac caccgcgcgg
     6601 atttctggcg tgccaagcta gtcgaccaat tctcatgttt gacagcttat catcgcagat
     6661 ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg taggtatgga agatctaacg
     6721 tactactgta catataacca ctggttttat atacagctac tgtacatata accactggtt
     6781 ttatatacag ctactgtaca tataaccact ggttttatat acagcaccat gagatctata
     6841 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
     6901 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacacg agtttactcc
     6961 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgatgtc
     7021 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
     7081 agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttatccct
     7141 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     7201 aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
     7261 cgcctggaga attcgagctc ggtacccgcc accatggtga gcaagggcga ggagctgttc
     7321 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     7381 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     7441 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     7501 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     7561 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     7621 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     7681 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     7741 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     7801 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     7861 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     7921 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     7981 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagg ccggcaaggc
     8041 cggatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     8101 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     8161 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     8221 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     8281 atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     8341 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcaggcgtc
     8401 agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg actctagagg atcgatgccc
     8461 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     8521 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     8581 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     8641 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     8701 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     8761 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     8821 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     8881 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     8941 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     9001 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     9061 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     9121 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     9181 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     9241 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     9301 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     9361 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     9421 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     9481 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     9541 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     9601 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     9661 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     9721 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     9781 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     9841 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     9901 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     9961 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
    10021 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
    10081 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
    10141 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
    10201 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
    10261 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
    10321 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
    10381 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
    10441 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
    10501 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
    10561 gtagcatatg ctatcctcat gcatatacag tcagcatatg atacccagta gtagagtggg
    10621 agtgctatcc tttgcatatg ccgccacctc ccaagggggc gtgaattttc gctgcttgtc
    10681 cttttcctgc tggttgctcc cattcttagg tgaatttaag gaggccaggc taaagccgtc
    10741 gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga gaaggtgttg
    10801 agcgcggagc tgagtgacgt ga
//
