LOCUS       pIG-602_NY-ESO-1_TCR_RF 5650 bp ds-DNA     circular     23-JUL-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1651..1952
                     /label="5' Homology Arm"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1807..1822
                     /label="Mismatch Region"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1953..1973
                     /label="Link 1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1974..1991
                     /label="5' Barcode"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1992..2051
                     /label="E2A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2052..2756
                     /label="RFP"
                     /ApEinfo_revcolor="#ff1abb"
                     /ApEinfo_fwdcolor="#ff1abb"
     misc_feature    2757..2768
                     /label="Furin"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    2769..2780
                     /label="GSGS"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2781..2798
                     /label="3' Barcode"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2799..2819
                     /label="Link 2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2820..2873
                     /label="T2A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2874..3806
                     /label="NY-ESO-1 TCR Beta"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3807..3818
                     /label="Furin"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    3819..3830
                     /label="SGSG"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    3831..3887
                     /label="P2A"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    3888..4310
                     /label="NY-ESO-1 TCR Alpha"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4310..4310
                     /label="C>G PAM Del"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4311..4614
                     /label="3' Homology Arm"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4615..1650
                     /label="PUC19"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
       61 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
      121 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
      181 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
      241 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
      301 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
      361 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
      421 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
      481 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
      541 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
      601 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
      661 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
      721 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
      781 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
      841 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
      901 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
      961 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     1021 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     1081 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA
     1141 TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT
     1201 AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC
     1261 CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC
     1321 AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT
     1381 GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA
     1441 TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCACCAT TCAGGCTGCG CAACTGTTGG
     1501 GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT
     1561 GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG
     1621 GCCAGTGAAT TCGAGCTCGG TACCCGGGGA TTTCAGGTTT CCTTGAGTGG CAGGCCAGGC
     1681 CTGGCCGTGA ACGTTCACTG AAATCATGGC CTCTTGGCCA AGATTGATAG CTTGTGCCTG
     1741 TCCCTGAGTC CCAGTCCATC ACGAGCAGCT GGTTTCTAAG ATGCTATTTC CCGTATAAAG
     1801 CATGAGCACG TGCATTCGCA CGCCCACAGA GCCCCGCCCT TGTCCATCAC TGGCATCTGG
     1861 ACTCCAGCCT GGGTTGGGGC AAAGAGGGAA ATGAGATCAT GTCCTAACCC TGATCCTCTT
     1921 GTCCCACAGA TATCCAGAAC CCTGACCCTG CCGGATCGGG TGGGACTAGT GGCGGTGGTG
     1981 GTAGTGGAGG Gcagtgcacc aactatgcgc tgctgaaact ggcgggcgat gtggaaagca
     2041 acccgggccc ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     2101 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagGGTgagG
     2161 GAgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     2221 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     2281 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     2341 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     2401 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     2461 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     2521 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     2581 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     2641 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     2701 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagAGGG
     2761 CTAAGAGAGG TTCAGGATCT GGTGGTGGTA GTGGGGGAgg aaccggtGCT ggaagtggtG
     2821 AGGGCAGGGG ATCTCTCCTT ACTTGTGGCG ACGTGGAGGA GAACCCCGGC CCCATGAGCA
     2881 TCGGCCTCCT GTGCTGTGCA GCCTTGTCTC TCCTGTGGGC AGGTCCAGTG AATGCTGGTG
     2941 TCACTCAGAC CCCAAAATTC CAGGTCCTGA AGACAGGACA GAGCATGACA CTGCAGTGTG
     3001 CCCAGGATAT GAACCATGAA TACATGTCCT GGTATCGACA AGACCCAGGC ATGGGGCTGA
     3061 GGCTGATTCA TTACTCAGTT GGTGCTGGTA TCACTGACCA AGGAGAAGTC CCCAATGGCT
     3121 ACAATGTCTC CAGATCAACC ACAGAGGATT TCCCGCTCAG GCTGCTGTCG GCTGCTCCCT
     3181 CCCAGACATC TGTGTACTTC TGTGCCAGCA GTTACGTCGG GAACACCGGG GAGCTGTTTT
     3241 TTGGAGAAGG CTCTAGGCTG ACCGTACTGG AGGACCTGAA AAACGTGTTC CCACCCGAGG
     3301 TCGCTGTGTT TGAGCCATCA GAAGCAGAGA TCTCCCACAC CCAAAAGGCC ACACTGGTAT
     3361 GCCTGGCCAC AGGCTTCTAC CCCGACCACG TGGAGCTGAG CTGGTGGGTG AATGGGAAGG
     3421 AGGTGCACAG TGGGGTCAGC ACAGACCCGC AGCCCCTCAA GGAGCAGCCC GCCCTCAATG
     3481 ACTCCAGATA CTGCCTGAGC AGCCGCCTGA GGGTCTCGGC CACCTTCTGG CAGAACCCCC
     3541 GCAACCACTT CCGCTGTCAA GTCCAGTTCT ACGGGCTCTC GGAGAATGAC GAGTGGACCC
     3601 AGGATAGGGC CAAACCCGTC ACCCAGATCG TCAGCGCCGA GGCCTGGGGT AGAGCAGACT
     3661 GTGGCTTCAC CTCCGAGTCT TACCAGCAAG GGGTCCTGTC TGCCACCATC CTCTATGAGA
     3721 TCTTGCTAGG GAAGGCCACC TTGTATGCCG TGCTGGTCAG TGCCCTCGTG CTGATGGCTA
     3781 TGGTCAAGAG AAAGGATTCC AGAGGCCGGG CCAAGCGGTC CGGATCCGGA GCCACCAACT
     3841 TCAGCCTGCT GAAGCAGGCC GGCGACGTGG AGGAGAACCC CGGCCCCATG GAGACCCTCT
     3901 TGGGCCTGCT TATCCTTTGG CTGCAGCTGC AATGGGTGAG CAGCAAACAG GAGGTGACGC
     3961 AGATTCCTGC AGCTCTGAGT GTCCCAGAAG GAGAAAACTT GGTTCTCAAC TGCAGTTTCA
     4021 CTGATAGCGC TATTTACAAC CTCCAGTGGT TTAGGCAGGA CCCTGGGAAA GGTCTCACAT
     4081 CTCTGTTGCT TATTCAGTCA AGTCAGAGAG AGCAAACAAG TGGAAGACTT AATGCCTCGC
     4141 TGGATAAATC ATCAGGACGT AGTACTTTAT ACATTGCAGC TTCTCAGCCT GGTGACTCAG
     4201 CCACCTACCT CTGTGCTGTG AGGCCCCTGT ACGGAGGAAG CTACATACCT ACATTTGGAA
     4261 GAGGAACCAG CCTTATTGTT CATCCGTATA TCCAGAACCC TGACCCTGCG GTGTACCAGC
     4321 TGAGAGACTC TAAATCCAGT GACAAGTCTG TCTGCCTATT CACCGATTTT GATTCTCAAA
     4381 CAAATGTGTC ACAAAGTAAG GATTCTGATG TGTATATCAC AGACAAAACT GTGCTAGACA
     4441 TGAGGTCTAT GGACTTCAAG AGCAACAGTG CTGTGGCCTG GAGCAACAAA TCTGACTTTG
     4501 CATGTGCAAA CGCCTTCAAC AACAGCATTA TTCCAGAAGA CACCTTCTTC CCCAGCCCAG
     4561 GTAAGGGCAG CTTTGGTGCC TTCGCAGGCT GTTTCCTTGC TTCAGGAATG GCCATCCTCT
     4621 AGAGTCGACC TGCAGGCATG CAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG
     4681 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC
     4741 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT
     4801 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG
     4861 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT
     4921 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC
     4981 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA
     5041 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     5101 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     5161 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     5221 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     5281 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     5341 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     5401 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     5461 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     5521 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     5581 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     5641 AGGATCTCAA
//