LOCUS       pIG-805_HA-GD2-28z_CAR_ 6963 bp ds-DNA     circular     23-JUL-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     None            1646..1947
                     /label="5' Homology Arm"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1802..1817
                     /label="Mismatch Region"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1948..2007
                     /label="E2A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2008..2382
                     /label="BATF"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2383..2394
                     /label="Furin"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    2395..2406
                     /label="GSGS"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2407..2424
                     /label="3' Barcode"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     None            2425..2445
                     /label="Link 2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     None            2446..2466
                     /label="Link 1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2467..2484
                     /label="5' Barcode"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     None            2485..2544
                     /label="E2A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2545..3249
                     /label="RFP"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3250..3261
                     /label="Furin"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    3262..3273
                     /label="GSGS"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     None            3274..3327
                     /label="T2A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3328..5535
                     /label="HA-GD2-28z CAR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    5536..5547
                     /label="Furin"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    5548..5559
                     /label="SGSG"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    5560..5616
                     /label="P2A"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    5619..5619
                     /label="C>G PAM Del"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     None            5620..5923
                     /label="3' Homology Arm"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    5924..1645
                     /label="PUC19"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
       61 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
      121 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
      181 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
      241 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
      301 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
      361 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
      421 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
      481 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
      541 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
      601 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
      661 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
      721 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
      781 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
      841 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
      901 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
      961 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     1021 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     1081 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA
     1141 TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT
     1201 AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC
     1261 CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC
     1321 AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT
     1381 GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA
     1441 TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG
     1501 GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT
     1561 GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG
     1621 GCCAGTGAAT TCGAGCTCGG TACCCTTTCA GGTTTCCTTG AGTGGCAGGC CAGGCCTGGC
     1681 CGTGAACGTT CACTGAAATC ATGGCCTCTT GGCCAAGATT GATAGCTTGT GCCTGTCCCT
     1741 GAGTCCCAGT CCATCACGAG CAGCTGGTTT CTAAGATGCT ATTTCCCGTA TAAAGCATGA
     1801 GCACGTGCAT TCGCACGCCC ACAGAGCCCC GCCCTTGTCC ATCACTGGCA TCTGGACTCC
     1861 AGCCTGGGTT GGGGCAAAGA GGGAAATGAG ATCATGTCCT AACCCTGATC CTCTTGTCCC
     1921 ACAGATATCC AGAACCCTGA CCCTGCCcag tgcaccaact atgcgctgct gaaactggcg
     1981 ggcgatgtgg aaagcaaccc gggcccgATG CCTCACAGCT CCGACAGCAG TGACTCCAGC
     2041 TTCAGCCGCT CTCCTCCCCC TGGCAAACAG GACTCATCTG ATGATGTGAG AAGAGTTCAG
     2101 AGGAGGGAGA AAAATCGTAT TGCCGCCCAG AAGAGCCGAC AGAGGCAGAC ACAGAAGGCC
     2161 GACACCCTGC ACCTGGAGAG CGAAGACCTG GAGAAACAGA ACGCGGCTCT ACGCAAGGAG
     2221 ATCAAGCAGC TCACAGAGGA ACTGAAGTAC TTCACGTCGG TGCTGAACAG CCACGAGCCC
     2281 CTGTGCTCGG TGCTGGCCGC CAGCACGCCC TCGCCCCCCG AGGTGGTGTA CAGCGCCCAC
     2341 GCATTCCACC AACCTCATGT CAGCTCCCCG CGCTTCCAGC CCAGGGCTAA GAGAGGTTCA
     2401 GGATCTGGCG GGGGCTCAGG GGGAggaacc ggtGCTggaa gtggtGGATC GGGTGGGACT
     2461 AGTGGCGGTG GTGGTAGTGG AGGGcagtgc accaactatg cgctgctgaa actggcgggc
     2521 gatgtggaaa gcaacccggg cccggtgagc aagggcgagg aggataacat ggccatcatc
     2581 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     2641 atcgagGGTg agGGAgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     2701 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     2761 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     2821 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     2881 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     2941 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     3001 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     3061 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     3121 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     3181 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     3241 ctgtacaagA GGGCTAAGAG AGGTTCAGGA TCtGAGGGCA GGGGATCTCT CCTTACTTGT
     3301 GGCGACGTGG AGGAGAACCC CGGCCCCATG GAATTCGGCC TGAGCTGGCT GTTCCTGGTG
     3361 GCCATCCTGA AGGGCGTGCA GTGCAGCAGA GACATCCTGC TGACCCAGAC CCCTCTGAGC
     3421 CTGCCTGTGT CTCTGGGCGA TCAGGCCAGC ATCAGCTGCA GATCCAGCCA GAGCCTGGTG
     3481 CACCGGAACG GCAACACCTA CCTGCACTGG TATCTGCAGA AGCCCGGCCA GAGCCCCAAG
     3541 CTGCTGATCC ACAAGGTGTC CAACCGGTTC AGCGGCGTGC CCGACAGATT TTCTGGCAGC
     3601 GGCTCCGGCA CCGACTTCAC CCTGAAGATC AGCCGGGTGG AAGCCGAGGA CCTGGGCGTG
     3661 TACTTCTGCA GCCAGTCCAC CCACGTGCCC CCCCTGACAT TTGGCGCCGG AACAAAGCTG
     3721 GAACTGAAGG GGGGAGGCGG ATCTGGCGGC GGAGGAAGTG GCGGAGGGGG ATCTGAAGTG
     3781 AAGCTGCAGC AGTCCGGCCC CAGCCTGGTG GAACCTGGCG CCTCTGTGAT GATCTCCTGC
     3841 AAGGCCAGCG GCAGCTCCTT CACCGGCTAC AACATGAACT GGGTGCGCCA GAACATCGGC
     3901 AAGAGCCTGG AATGGATCGG CGCCATCGAC CCCTACTACG GCGGCACCAG CTACAACCAG
     3961 AAGTTCAAGG GCAGAGCCAC CCTGACCGTG GACAAGAGCA GCAGCACCGC CTACATGCAC
     4021 CTGAAGTCCC TGACCAGCGA GGACAGCGCC GTGTACTACT GCGTGTCCGG CATGAAGTAC
     4081 TGGGGCCAGG GCACAAGCGT GACCGTGTCT AGCGCCAAGA CCACCCCCCC TAGCGTGTAC
     4141 GGAAGAGTGA CAGTGTCCTC TGCCGAGCCC AAGAGCTGCG ACAAGACCCA CACCTGTCCC
     4201 CCTTGTCCTG CCCCTCCTGT TGCAGGCCCT TCCGTGTTCC TGTTCCCCCC AAAGCCCAAG
     4261 GACACACTGA TGATCAGCAG AACCCCCGAA GTGACCTGCG TGGTGGTGGA CGTGTCCCAC
     4321 GAGGACCCAG AAGTGAAGTT CAATTGGTAC GTGGACGGCG TGGAAGTGCA CAACGCCAAG
     4381 ACAAAGCCCA GAGAGGAACA GTACCAAAGC ACCTACCGGG TGGTGTCCGT GCTGACCGTG
     4441 CTGCATCAGG ATTGGCTGAA CGGCAAAGAG TACAAGTGCA AAGTGTCCAA CAAGGCCCTG
     4501 CCTGCCCCCA TCGAGAAAAC CATCAGCAAG GCCAAGGGCC AGCCCCGCGA ACCCCAGGTG
     4561 TACACACTGC CCCCTAGCAG GGACGAGCTG ACCAAGAACC AGGTGTCCCT GACATGCCTC
     4621 GTGAAGGGCT TCTACCCCTC CGATATCGCC GTGGAATGGG AGAGCAACGG CCAGCCCGAG
     4681 AACAACTACA AGACAACCCC TCCCGTGCTG GACAGCGACG GCTCATTCTT CCTGTACAGC
     4741 AAGCTGACAG TGGATAAGTC CCGGTGGCAG CAGGGCAACG TGTTCAGCTG CTCCGTGATG
     4801 CACGAGGCCC TGCACAACCA CTACACCCAG AAAAGCCTGT CCCTGAGCCC CGGCAAGAAG
     4861 GACCCCAAAG CGGCCGCAAT TGAAGTTATG TATCCTCCTC CTTACCTAGA CAATGAGAAG
     4921 AGCAATGGAA CCATTATCCA TGTGAAAGGG AAACACCTTT GTCCAAGTCC CCTATTTCCC
     4981 GGACCTTCTA AGCCCTTTTG GGTGCTGGTG GTGGTTGGGG GAGTCCTGGC TTGCTATAGC
     5041 TTGCTAGTAA CAGTGGCCTT TATTATTTTC TGGGTGAGGA GTAAGAGGAG CAGGCTCCTG
     5101 CACAGTGACT ACATGAACAT GACTCCCCGC CGCCCCGGGC CCACCCGCAA GCATTACCAG
     5161 CCCTATGCCC CACCACGCGA CTTCGCAGCC TATCGCTCCA GAGTGAAGTT CAGCAGGAGC
     5221 GCAGACGCCC CCGCGTACCA GCAGGGCCAG AACCAGCTCT ATAACGAGCT CAATCTAGGA
     5281 CGAAGAGAGG AGTACGATGT TTTGGACAAG AGACGTGGCC GGGACCCTGA GATGGGGGGA
     5341 AAGCCGAGAA GGAAGAACCC TCAGGAAGGC CTGTACAATG AACTGCAGAA AGATAAGATG
     5401 GCGGAGGCCT ACAGTGAGAT TGGGATGAAA GGCGAGCGCC GGAGGGGCAA GGGGCACGAT
     5461 GGCCTTTACC AGGGTCTCAG TACAGCCACC AAGGACACCT ACGACGCCCT TCACATGCAG
     5521 GCCCTGCCCC CTCGCCGGGC CAAGCGGTCC GGATCCGGAG CCACCAACTT CAGCCTGCTG
     5581 AAGCAGGCCG GCGACGTGGA GGAGAACCCC GGCCCCGCGG TGTACCAGCT GAGAGACTCT
     5641 AAATCCAGTG ACAAGTCTGT CTGCCTATTC ACCGATTTTG ATTCTCAAAC AAATGTGTCA
     5701 CAAAGTAAGG ATTCTGATGT GTATATCACA GACAAAACTG TGCTAGACAT GAGGTCTATG
     5761 GACTTCAAGA GCAACAGTGC TGTGGCCTGG AGCAACAAAT CTGACTTTGC ATGTGCAAAC
     5821 GCCTTCAACA ACAGCATTAT TCCAGAAGAC ACCTTCTTCC CCAGCCCAGG TAAGGGCAGC
     5881 TTTGGTGCCT TCGCAGGCTG TTTCCTTGCT TCAGGAATGG CCAGGGGATC CTCTAGATCG
     5941 ACCTGCAGGC ATGCAAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT
     6001 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT
     6061 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG
     6121 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG
     6181 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG
     6241 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT
     6301 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC
     6361 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
     6421 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA
     6481 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT
     6541 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG
     6601 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC
     6661 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
     6721 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC
     6781 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG
     6841 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC
     6901 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT
     6961 CAA
//