LOCUS       pIG-836_NY-ESO-1_TCR_FA 5662 bp ds-DNA     circular     24-JUL-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1651..1952
                     /label="5' Homology Arm"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1807..1822
                     /label="Mismatch Region"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1953..1973
                     /label="Link 1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1974..1991
                     /label="5' Barcode"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1992..2051
                     /label="E2A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2052..2768
                     /label="FAS/OX40"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2769..2780
                     /label="Furin"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2781..2792
                     /label="GSGS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2793..2810
                     /label="3' Barcode"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2811..2831
                     /label="Link 2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2832..2885
                     /label="T2A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2886..3818
                     /label="NY-ESO-1 TCR Beta"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3819..3830
                     /label="Furin"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    3831..3842
                     /label="SGSG"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    3843..3899
                     /label="P2A"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    3900..4322
                     /label="NYESO-1 TCR Alpha"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4322..4322
                     /label="C>G PAM Del"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4323..4626
                     /label="3' Homology Arm"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4627..1650
                     /label="PUC19"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
       61 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
      121 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
      181 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
      241 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
      301 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
      361 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
      421 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
      481 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
      541 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
      601 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
      661 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
      721 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
      781 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
      841 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
      901 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
      961 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     1021 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     1081 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA
     1141 TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT
     1201 AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC
     1261 CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC
     1321 AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT
     1381 GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA
     1441 TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCACCAT TCAGGCTGCG CAACTGTTGG
     1501 GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT
     1561 GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG
     1621 GCCAGTGAAT TCGAGCTCGG TACCCGGGGA TTTCAGGTTT CCTTGAGTGG CAGGCCAGGC
     1681 CTGGCCGTGA ACGTTCACTG AAATCATGGC CTCTTGGCCA AGATTGATAG CTTGTGCCTG
     1741 TCCCTGAGTC CCAGTCCATC ACGAGCAGCT GGTTTCTAAG ATGCTATTTC CCGTATAAAG
     1801 CATGAGCACG TGCATTCGCA CGCCCACAGA GCCCCGCCCT TGTCCATCAC TGGCATCTGG
     1861 ACTCCAGCCT GGGTTGGGGC AAAGAGGGAA ATGAGATCAT GTCCTAACCC TGATCCTCTT
     1921 GTCCCACAGA TATCCAGAAC CCTGACCCTG CCGGATCGGG TGGGACTAGT GGCGGTGGAG
     1981 GGAGCGGCGG ACAGTGCACC AACTATGCGC TGCTGAAACT GGCGGGCGAT GTGGAAAGCA
     2041 ACCCGGGCCC GATGCTGGGC ATCTGGACCC TCCTACCTCT GGTTCTTACG TCTGTTGCTA
     2101 GATTATCGTC CAAAAGTGTT AATGCCCAAG TGACTGACAT CAACTCCAAG GGATTGGAAT
     2161 TGAGGAAGAC TGTTACTACA GTTGAGACTC AGAACTTGGA AGGCCTGCAT CATGATGGCC
     2221 AATTCTGCCA TAAGCCCTGT CCTCCAGGTG AAAGGAAAGC TAGGGACTGC ACAGTCAATG
     2281 GGGATGAACC AGACTGCGTG CCCTGCCAAG AAGGGAAGGA GTACACAGAC AAAGCCCATT
     2341 TTTCTTCCAA ATGCAGAAGA TGTAGATTGT GTGATGAAGG ACATGGCTTA GAAGTGGAAA
     2401 TAAACTGCAC CCGGACCCAG AATACCAAGT GCAGATGTAA ACCAAACTTT TTTTGTAACT
     2461 CTACTGTATG TGAACACTGT GACCCTTGCA CCAAATGTGA ACATGGAATC ATCAAGGAAT
     2521 GCACACTCAC CAGCAACACC AAGTGCAAAG AGGAAGGATC CAGATCTAAC TTGGGGTGGC
     2581 TTTGTCTTCT TCTTTTGCCA ATTCCACTAA TTGTTTGGGT GAAGAGAAAG GAAGTACAGA
     2641 AAGCCCTGTA CCTGCTCCGG AGGGACCAGA GGCTGCCCCC CGATGCCCAC AAGCCCCCTG
     2701 GGGGAGGCAG TTTCCGGACC CCCATCCAAG AGGAGCAGGC CGACGCCCAC TCCACCCTGG
     2761 CCAAGATCAG GGCTAAGAGA GGTTCAGGAT CTGGCGGTGG ATCTGGCGGA ggaaccggtG
     2821 CTggaagtgg tGAGGGCAGG GGATCTCTCC TTACTTGTGG CGACGTGGAG GAGAACCCCG
     2881 GCCCCATGAG CATCGGCCTC CTGTGCTGTG CAGCCTTGTC TCTCCTGTGG GCAGGTCCAG
     2941 TGAATGCTGG TGTCACTCAG ACCCCAAAAT TCCAGGTCCT GAAGACAGGA CAGAGCATGA
     3001 CACTGCAGTG TGCCCAGGAT ATGAACCATG AATACATGTC CTGGTATCGA CAAGACCCAG
     3061 GCATGGGGCT GAGGCTGATT CATTACTCAG TTGGTGCTGG TATCACTGAC CAAGGAGAAG
     3121 TCCCCAATGG CTACAATGTC TCCAGATCAA CCACAGAGGA TTTCCCGCTC AGGCTGCTGT
     3181 CGGCTGCTCC CTCCCAGACA TCTGTGTACT TCTGTGCCAG CAGTTACGTC GGGAACACCG
     3241 GGGAGCTGTT TTTTGGAGAA GGCTCTAGGC TGACCGTACT GGAGGACCTG AAAAACGTGT
     3301 TCCCACCCGA GGTCGCTGTG TTTGAGCCAT CAGAAGCAGA GATCTCCCAC ACCCAAAAGG
     3361 CCACACTGGT ATGCCTGGCC ACAGGCTTCT ACCCCGACCA CGTGGAGCTG AGCTGGTGGG
     3421 TGAATGGGAA GGAGGTGCAC AGTGGGGTCA GCACAGACCC GCAGCCCCTC AAGGAGCAGC
     3481 CCGCCCTCAA TGACTCCAGA TACTGCCTGA GCAGCCGCCT GAGGGTCTCG GCCACCTTCT
     3541 GGCAGAACCC CCGCAACCAC TTCCGCTGTC AAGTCCAGTT CTACGGGCTC TCGGAGAATG
     3601 ACGAGTGGAC CCAGGATAGG GCCAAACCCG TCACCCAGAT CGTCAGCGCC GAGGCCTGGG
     3661 GTAGAGCAGA CTGTGGCTTC ACCTCCGAGT CTTACCAGCA AGGGGTCCTG TCTGCCACCA
     3721 TCCTCTATGA GATCTTGCTA GGGAAGGCCA CCTTGTATGC CGTGCTGGTC AGTGCCCTCG
     3781 TGCTGATGGC TATGGTCAAG AGAAAGGATT CCAGAGGCCG GGCCAAGCGG TCCGGATCCG
     3841 GAGCCACCAA CTTCAGCCTG CTGAAGCAGG CCGGCGACGT GGAGGAGAAC CCCGGCCCCA
     3901 TGGAGACCCT CTTGGGCCTG CTTATCCTTT GGCTGCAGCT GCAATGGGTG AGCAGCAAAC
     3961 AGGAGGTGAC GCAGATTCCT GCAGCTCTGA GTGTCCCAGA AGGAGAAAAC TTGGTTCTCA
     4021 ACTGCAGTTT CACTGATAGC GCTATTTACA ACCTCCAGTG GTTTAGGCAG GACCCTGGGA
     4081 AAGGTCTCAC ATCTCTGTTG CTTATTCAGT CAAGTCAGAG AGAGCAAACA AGTGGAAGAC
     4141 TTAATGCCTC GCTGGATAAA TCATCAGGAC GTAGTACTTT ATACATTGCA GCTTCTCAGC
     4201 CTGGTGACTC AGCCACCTAC CTCTGTGCTG TGAGGCCCCT GTACGGAGGA AGCTACATAC
     4261 CTACATTTGG AAGAGGAACC AGCCTTATTG TTCATCCGTA TATCCAGAAC CCTGACCCTG
     4321 CGGTGTACCA GCTGAGAGAC TCTAAATCCA GTGACAAGTC TGTCTGCCTA TTCACCGATT
     4381 TTGATTCTCA AACAAATGTG TCACAAAGTA AGGATTCTGA TGTGTATATC ACAGACAAAA
     4441 CTGTGCTAGA CATGAGGTCT ATGGACTTCA AGAGCAACAG TGCTGTGGCC TGGAGCAACA
     4501 AATCTGACTT TGCATGTGCA AACGCCTTCA ACAACAGCAT TATTCCAGAA GACACCTTCT
     4561 TCCCCAGCCC AGGTAAGGGC AGCTTTGGTG CCTTCGCAGG CTGTTTCCTT GCTTCAGGAA
     4621 TGGCCATCCT CTAGAGTCGA CCTGCAGGCA TGCAAGCTTG GCGTAATCAT GGTCATAGCT
     4681 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT
     4741 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC
     4801 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG
     4861 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT
     4921 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
     4981 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
     5041 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
     5101 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     5161 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     5221 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     5281 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     5341 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     5401 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     5461 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     5521 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     5581 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     5641 GCGCAGAAAA AAAGGATCTC AA
//