LOCUS       pIG-851_HA-GD2-28z_HA-T 6895 bp ds-DNA     circular     24-JUL-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1646..1947
                     /label="5' Homology Arm"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1802..1817
                     /label="Mismatch Region"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1948..1968
                     /label="Link 1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1969..1986
                     /label="5' Barcode"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     None            1987..2046
                     /label="E2A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2047..3141
                     /label="TFAP4"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2050..2076
                     /label="HA1"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2050..2130
                     /label="3xHA"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="(Plasmid #85719) Addgene"
     misc_feature    2077..2103
                     /label="HA2"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2104..2130
                     /label="HA3"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3142..3153
                     /label="Furin"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3154..3165
                     /label="GSGS"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3166..3183
                     /label="3' Barcode"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3184..3204
                     /label="Link 2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3205..3258
                     /label="T2A"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3259..5466
                     /label="HA-GD2-28z CAR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    5467..5478
                     /label="Furin"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    5479..5490
                     /label="SGSG"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    5491..5547
                     /label="P2A"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    5550..5550
                     /label="C>G PAM Del"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    5551..5854
                     /label="3' Homology Arm"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    5855..1645
                     /label="PUC19"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
       61 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
      121 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
      181 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
      241 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
      301 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
      361 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
      421 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
      481 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
      541 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
      601 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
      661 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
      721 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
      781 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
      841 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
      901 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
      961 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     1021 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     1081 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA
     1141 TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT
     1201 AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC
     1261 CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC
     1321 AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT
     1381 GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA
     1441 TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG
     1501 GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT
     1561 GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG
     1621 GCCAGTGAAT TCGAGCTCGG TACCCTTTCA GGTTTCCTTG AGTGGCAGGC CAGGCCTGGC
     1681 CGTGAACGTT CACTGAAATC ATGGCCTCTT GGCCAAGATT GATAGCTTGT GCCTGTCCCT
     1741 GAGTCCCAGT CCATCACGAG CAGCTGGTTT CTAAGATGCT ATTTCCCGTA TAAAGCATGA
     1801 GCACGTGCAT TCGCACGCCC ACAGAGCCCC GCCCTTGTCC ATCACTGGCA TCTGGACTCC
     1861 AGCCTGGGTT GGGGCAAAGA GGGAAATGAG ATCATGTCCT AACCCTGATC CTCTTGTCCC
     1921 ACAGATATCC AGAACCCTGA CCCTGCCGGA TCGGGTGGGA CTAGTGGCGG TGGAGGATCT
     1981 GGAGGACAGT GCACCAACTA TGCGCTGCTG AAACTGGCGG GCGATGTGGA AAGCAACCCG
     2041 GGCCCGATGt acccatacga tgttccagat tacgcttatc cttatgacgt acctgactat
     2101 gcataccctt atgatgtacc agactacgct GAGTATTTCA TGGTGCCCAC TCAGAAGGTG
     2161 CCCTCTTTGC AACATTTCAG GAAAACAGAG AAAGAAGTGA TAGGAGGGCT CTGTAGCCTT
     2221 GCCAACATTC CACTAACCCC CGAGACTCAG CGGGACCAGG AGCGGCGGAT TCGGCGGGAG
     2281 ATCGCCAACA GCAACGAGCG GAGACGCATG CAGAGCATCA ACGCGGGATT CCAGTCCCTC
     2341 AAGACCCTCA TCCCCCACAC AGACGGAGAG AAGCTCAGCA AGGCAGCCAT TCTCCAGCAG
     2401 ACAGCCGAGT ACATCTTCTC CCTGGAGCAG GAGAAGACCA GGCTCTTGCA GCAGAACACA
     2461 CAGCTCAAGC GCTTCATCCA GGAGCTGAGC GGCTCGTCCC CCAAGCGACG GCGGGCAGAG
     2521 GACAAGGACG AAGGCATAGG CTCCCCGGAC ATCTGGGAGG ACGAGAAGGC GGAGGACCTG
     2581 CGGCGGGAGA TGATTGAGCT GCGGCAGCAG CTGGACAAGG AGCGCTCGGT GCGCATGATG
     2641 CTGGAGGAGC AGGTGCGCTC GCTGGAGGCC CACATGTACC CGGAAAAGCT CAAGGTGATT
     2701 GCGCAGCAGG TGCAGCTGCA GCAGCAGCAG GAACAGGTGA GGCTGCTGCA CCAGGAGAAG
     2761 CTGGAGCGGG AACAGCAGCA GCTGCGGACC CAGCTTCTGC CCCCTCCGGC CCCCACCCAC
     2821 CACCCCACGG TGATCGTGCC AGCACCGCCT CCTCCTCCCT CCCACCACAT CAATGTCGTC
     2881 ACCATGGGCC CCTCCTCGGT CATCAACTCT GTTTCCACAT CCCGGCAAAA TCTGGACACC
     2941 ATCGTGCAGG CAATCCAGCA CATCGAGGGC ACCCAGGAAA AGCAGGAGCT GGAGGAGGAG
     3001 CAGCGGCGAG CTGTCATCGT GAAGCCTGTC CGCAGCTGCC CGGAGGCCCC CACCTCTGAC
     3061 ACCGCCTCCG ACTCCGAGGC CTCAGACAGT GACGCCATGG ACCAGAGCCG GGAGGAGCCG
     3121 TCGGGGGACG GGGAGCTTCC CAGGGCTAAG AGAGGTTCAG GATCTGGAGG CGGATCTGGG
     3181 GGAggaaccg gtGCTggaag tggtGAGGGC AGGGGATCTC TCCTTACTTG TGGCGACGTG
     3241 GAGGAGAACC CCGGCCCCAT GGAATTCGGC CTGAGCTGGC TGTTCCTGGT GGCCATCCTG
     3301 AAGGGCGTGC AGTGCAGCAG AGACATCCTG CTGACCCAGA CCCCTCTGAG CCTGCCTGTG
     3361 TCTCTGGGCG ATCAGGCCAG CATCAGCTGC AGATCCAGCC AGAGCCTGGT GCACCGGAAC
     3421 GGCAACACCT ACCTGCACTG GTATCTGCAG AAGCCCGGCC AGAGCCCCAA GCTGCTGATC
     3481 CACAAGGTGT CCAACCGGTT CAGCGGCGTG CCCGACAGAT TTTCTGGCAG CGGCTCCGGC
     3541 ACCGACTTCA CCCTGAAGAT CAGCCGGGTG GAAGCCGAGG ACCTGGGCGT GTACTTCTGC
     3601 AGCCAGTCCA CCCACGTGCC CCCCCTGACA TTTGGCGCCG GAACAAAGCT GGAACTGAAG
     3661 GGGGGAGGCG GATCTGGCGG CGGAGGAAGT GGCGGAGGGG GATCTGAAGT GAAGCTGCAG
     3721 CAGTCCGGCC CCAGCCTGGT GGAACCTGGC GCCTCTGTGA TGATCTCCTG CAAGGCCAGC
     3781 GGCAGCTCCT TCACCGGCTA CAACATGAAC TGGGTGCGCC AGAACATCGG CAAGAGCCTG
     3841 GAATGGATCG GCGCCATCGA CCCCTACTAC GGCGGCACCA GCTACAACCA GAAGTTCAAG
     3901 GGCAGAGCCA CCCTGACCGT GGACAAGAGC AGCAGCACCG CCTACATGCA CCTGAAGTCC
     3961 CTGACCAGCG AGGACAGCGC CGTGTACTAC TGCGTGTCCG GCATGAAGTA CTGGGGCCAG
     4021 GGCACAAGCG TGACCGTGTC TAGCGCCAAG ACCACCCCCC CTAGCGTGTA CGGAAGAGTG
     4081 ACAGTGTCCT CTGCCGAGCC CAAGAGCTGC GACAAGACCC ACACCTGTCC CCCTTGTCCT
     4141 GCCCCTCCTG TTGCAGGCCC TTCCGTGTTC CTGTTCCCCC CAAAGCCCAA GGACACACTG
     4201 ATGATCAGCA GAACCCCCGA AGTGACCTGC GTGGTGGTGG ACGTGTCCCA CGAGGACCCA
     4261 GAAGTGAAGT TCAATTGGTA CGTGGACGGC GTGGAAGTGC ACAACGCCAA GACAAAGCCC
     4321 AGAGAGGAAC AGTACCAAAG CACCTACCGG GTGGTGTCCG TGCTGACCGT GCTGCATCAG
     4381 GATTGGCTGA ACGGCAAAGA GTACAAGTGC AAAGTGTCCA ACAAGGCCCT GCCTGCCCCC
     4441 ATCGAGAAAA CCATCAGCAA GGCCAAGGGC CAGCCCCGCG AACCCCAGGT GTACACACTG
     4501 CCCCCTAGCA GGGACGAGCT GACCAAGAAC CAGGTGTCCC TGACATGCCT CGTGAAGGGC
     4561 TTCTACCCCT CCGATATCGC CGTGGAATGG GAGAGCAACG GCCAGCCCGA GAACAACTAC
     4621 AAGACAACCC CTCCCGTGCT GGACAGCGAC GGCTCATTCT TCCTGTACAG CAAGCTGACA
     4681 GTGGATAAGT CCCGGTGGCA GCAGGGCAAC GTGTTCAGCT GCTCCGTGAT GCACGAGGCC
     4741 CTGCACAACC ACTACACCCA GAAAAGCCTG TCCCTGAGCC CCGGCAAGAA GGACCCCAAA
     4801 GCGGCCGCAA TTGAAGTTAT GTATCCTCCT CCTTACCTAG ACAATGAGAA GAGCAATGGA
     4861 ACCATTATCC ATGTGAAAGG GAAACACCTT TGTCCAAGTC CCCTATTTCC CGGACCTTCT
     4921 AAGCCCTTTT GGGTGCTGGT GGTGGTTGGG GGAGTCCTGG CTTGCTATAG CTTGCTAGTA
     4981 ACAGTGGCCT TTATTATTTT CTGGGTGAGG AGTAAGAGGA GCAGGCTCCT GCACAGTGAC
     5041 TACATGAACA TGACTCCCCG CCGCCCCGGG CCCACCCGCA AGCATTACCA GCCCTATGCC
     5101 CCACCACGCG ACTTCGCAGC CTATCGCTCC AGAGTGAAGT TCAGCAGGAG CGCAGACGCC
     5161 CCCGCGTACC AGCAGGGCCA GAACCAGCTC TATAACGAGC TCAATCTAGG ACGAAGAGAG
     5221 GAGTACGATG TTTTGGACAA GAGACGTGGC CGGGACCCTG AGATGGGGGG AAAGCCGAGA
     5281 AGGAAGAACC CTCAGGAAGG CCTGTACAAT GAACTGCAGA AAGATAAGAT GGCGGAGGCC
     5341 TACAGTGAGA TTGGGATGAA AGGCGAGCGC CGGAGGGGCA AGGGGCACGA TGGCCTTTAC
     5401 CAGGGTCTCA GTACAGCCAC CAAGGACACC TACGACGCCC TTCACATGCA GGCCCTGCCC
     5461 CCTCGCCGGG CCAAGCGGTC CGGATCCGGA GCCACCAACT TCAGCCTGCT GAAGCAGGCC
     5521 GGCGACGTGG AGGAGAACCC CGGCCCCGCG GTGTACCAGC TGAGAGACTC TAAATCCAGT
     5581 GACAAGTCTG TCTGCCTATT CACCGATTTT GATTCTCAAA CAAATGTGTC ACAAAGTAAG
     5641 GATTCTGATG TGTATATCAC AGACAAAACT GTGCTAGACA TGAGGTCTAT GGACTTCAAG
     5701 AGCAACAGTG CTGTGGCCTG GAGCAACAAA TCTGACTTTG CATGTGCAAA CGCCTTCAAC
     5761 AACAGCATTA TTCCAGAAGA CACCTTCTTC CCCAGCCCAG GTAAGGGCAG CTTTGGTGCC
     5821 TTCGCAGGCT GTTTCCTTGC TTCAGGAATG GCCAGGGGAT CCTCTAGAGT CGACCTGCAG
     5881 GCATGCAAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT
     5941 CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG
     6001 AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT
     6061 GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG
     6121 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC
     6181 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG
     6241 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT
     6301 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     6361 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     6421 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     6481 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     6541 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     6601 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     6661 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     6721 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     6781 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     6841 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAA
//