LOCUS       pIG-874_HA-GD2-28z_CAR_ 6615 bp ds-DNA     circular     24-JUL-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1646..1947
                     /label="5' Homology Arm"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1802..1817
                     /label="Mismatch Region"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1948..1968
                     /label="Link 1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1969..1986
                     /label="5' Barcode"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     None            1987..2046
                     /label="E2A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2047..2862
                     /label="IL2RA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2863..2874
                     /label="Furin"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    2875..2886
                     /label="GSGS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2887..2904
                     /label="3' Barcode"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2905..2925
                     /label="Link 2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2926..2979
                     /label="T2A"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2980..5187
                     /label="HA-GD2-28z CAR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    5188..5199
                     /label="Furin"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    5200..5211
                     /label="SGSG"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     misc_feature    5212..5268
                     /label="P2A"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    5271..5271
                     /label="C>G PAM Del"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    5272..5575
                     /label="3' Homology Arm"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    5576..1645
                     /label="PUC19"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
       61 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
      121 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
      181 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
      241 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
      301 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
      361 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
      421 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
      481 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
      541 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
      601 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
      661 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
      721 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
      781 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
      841 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
      901 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
      961 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     1021 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     1081 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA
     1141 TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT
     1201 AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC
     1261 CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC
     1321 AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT
     1381 GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA
     1441 TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG
     1501 GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT
     1561 GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG
     1621 GCCAGTGAAT TCGAGCTCGG TACCCTTTCA GGTTTCCTTG AGTGGCAGGC CAGGCCTGGC
     1681 CGTGAACGTT CACTGAAATC ATGGCCTCTT GGCCAAGATT GATAGCTTGT GCCTGTCCCT
     1741 GAGTCCCAGT CCATCACGAG CAGCTGGTTT CTAAGATGCT ATTTCCCGTA TAAAGCATGA
     1801 GCACGTGCAT TCGCACGCCC ACAGAGCCCC GCCCTTGTCC ATCACTGGCA TCTGGACTCC
     1861 AGCCTGGGTT GGGGCAAAGA GGGAAATGAG ATCATGTCCT AACCCTGATC CTCTTGTCCC
     1921 ACAGATATCC AGAACCCTGA CCCTGCCGGA TCGGGTGGGA CTAGTGGCGG GGGTGGGAGC
     1981 GGTGGACAGT GCACCAACTA TGCGCTGCTG AAACTGGCGG GCGATGTGGA AAGCAACCCG
     2041 GGCCCGATGG ATTCATACCT GCTGATGTGG GGACTGCTCA CGTTCATCAT GGTGCCTGGC
     2101 TGCCAGGCAG AGCTCTGTGA CGATGACCCG CCAGAGATCC CACACGCCAC ATTCAAAGCC
     2161 ATGGCCTACA AGGAAGGAAC CATGTTGAAC TGTGAATGCA AGAGAGGTTT CCGCAGAATA
     2221 AAAAGCGGGT CACTCTATAT GCTCTGTACA GGAAACTCTA GCCACTCGTC CTGGGACAAC
     2281 CAATGTCAAT GCACAAGCTC TGCCACTCGG AACACAACGA AACAAGTGAC ACCTCAACCT
     2341 GAAGAACAGA AAGAAAGGAA AACCACAGAA ATGCAAAGTC CAATGCAGCC AGTGGACCAA
     2401 GCGAGCCTTC CAGGTCACTG CAGGGAACCT CCACCATGGG AAAATGAAGC CACAGAGAGA
     2461 ATTTATCATT TCGTGGTGGG GCAGATGGTT TATTATCAGT GCGTCCAGGG ATACAGGGCT
     2521 CTACACAGAG GTCCTGCTGA GAGCGTCTGC AAAATGACCC ACGGGAAGAC AAGGTGGACC
     2581 CAGCCCCAGC TCATATGCAC AGGTGAAATG GAGACCAGTC AGTTTCCAGG TGAAGAGAAG
     2641 CCTCAGGCAA GCCCCGAAGG CCGTCCTGAG AGTGAGACTT CCTGCCTCGT CACAACAACA
     2701 GATTTTCAAA TACAGACAGA AATGGCTGCA ACCATGGAGA CGTCCATATT TACAACAGAG
     2761 TACCAGGTAG CAGTGGCCGG CTGTGTTTTC CTGCTGATCA GCGTCCTCCT CCTGAGTGGG
     2821 CTCACCTGGC AGCGGAGACA GAGGAAGAGT AGAAGAACAA TCAGGGCTAA GAGAGGTTCA
     2881 GGATCTGGTG GAGGTTCGGG AGGGggaacc ggtGCTggaa gtggtGAGGG CAGGGGATCT
     2941 CTCCTTACTT GTGGCGACGT GGAGGAGAAC CCCGGCCCCA TGGAATTCGG CCTGAGCTGG
     3001 CTGTTCCTGG TGGCCATCCT GAAGGGCGTG CAGTGCAGCA GAGACATCCT GCTGACCCAG
     3061 ACCCCTCTGA GCCTGCCTGT GTCTCTGGGC GATCAGGCCA GCATCAGCTG CAGATCCAGC
     3121 CAGAGCCTGG TGCACCGGAA CGGCAACACC TACCTGCACT GGTATCTGCA GAAGCCCGGC
     3181 CAGAGCCCCA AGCTGCTGAT CCACAAGGTG TCCAACCGGT TCAGCGGCGT GCCCGACAGA
     3241 TTTTCTGGCA GCGGCTCCGG CACCGACTTC ACCCTGAAGA TCAGCCGGGT GGAAGCCGAG
     3301 GACCTGGGCG TGTACTTCTG CAGCCAGTCC ACCCACGTGC CCCCCCTGAC ATTTGGCGCC
     3361 GGAACAAAGC TGGAACTGAA GGGGGGAGGC GGATCTGGCG GCGGAGGAAG TGGCGGAGGG
     3421 GGATCTGAAG TGAAGCTGCA GCAGTCCGGC CCCAGCCTGG TGGAACCTGG CGCCTCTGTG
     3481 ATGATCTCCT GCAAGGCCAG CGGCAGCTCC TTCACCGGCT ACAACATGAA CTGGGTGCGC
     3541 CAGAACATCG GCAAGAGCCT GGAATGGATC GGCGCCATCG ACCCCTACTA CGGCGGCACC
     3601 AGCTACAACC AGAAGTTCAA GGGCAGAGCC ACCCTGACCG TGGACAAGAG CAGCAGCACC
     3661 GCCTACATGC ACCTGAAGTC CCTGACCAGC GAGGACAGCG CCGTGTACTA CTGCGTGTCC
     3721 GGCATGAAGT ACTGGGGCCA GGGCACAAGC GTGACCGTGT CTAGCGCCAA GACCACCCCC
     3781 CCTAGCGTGT ACGGAAGAGT GACAGTGTCC TCTGCCGAGC CCAAGAGCTG CGACAAGACC
     3841 CACACCTGTC CCCCTTGTCC TGCCCCTCCT GTTGCAGGCC CTTCCGTGTT CCTGTTCCCC
     3901 CCAAAGCCCA AGGACACACT GATGATCAGC AGAACCCCCG AAGTGACCTG CGTGGTGGTG
     3961 GACGTGTCCC ACGAGGACCC AGAAGTGAAG TTCAATTGGT ACGTGGACGG CGTGGAAGTG
     4021 CACAACGCCA AGACAAAGCC CAGAGAGGAA CAGTACCAAA GCACCTACCG GGTGGTGTCC
     4081 GTGCTGACCG TGCTGCATCA GGATTGGCTG AACGGCAAAG AGTACAAGTG CAAAGTGTCC
     4141 AACAAGGCCC TGCCTGCCCC CATCGAGAAA ACCATCAGCA AGGCCAAGGG CCAGCCCCGC
     4201 GAACCCCAGG TGTACACACT GCCCCCTAGC AGGGACGAGC TGACCAAGAA CCAGGTGTCC
     4261 CTGACATGCC TCGTGAAGGG CTTCTACCCC TCCGATATCG CCGTGGAATG GGAGAGCAAC
     4321 GGCCAGCCCG AGAACAACTA CAAGACAACC CCTCCCGTGC TGGACAGCGA CGGCTCATTC
     4381 TTCCTGTACA GCAAGCTGAC AGTGGATAAG TCCCGGTGGC AGCAGGGCAA CGTGTTCAGC
     4441 TGCTCCGTGA TGCACGAGGC CCTGCACAAC CACTACACCC AGAAAAGCCT GTCCCTGAGC
     4501 CCCGGCAAGA AGGACCCCAA AGCGGCCGCA ATTGAAGTTA TGTATCCTCC TCCTTACCTA
     4561 GACAATGAGA AGAGCAATGG AACCATTATC CATGTGAAAG GGAAACACCT TTGTCCAAGT
     4621 CCCCTATTTC CCGGACCTTC TAAGCCCTTT TGGGTGCTGG TGGTGGTTGG GGGAGTCCTG
     4681 GCTTGCTATA GCTTGCTAGT AACAGTGGCC TTTATTATTT TCTGGGTGAG GAGTAAGAGG
     4741 AGCAGGCTCC TGCACAGTGA CTACATGAAC ATGACTCCCC GCCGCCCCGG GCCCACCCGC
     4801 AAGCATTACC AGCCCTATGC CCCACCACGC GACTTCGCAG CCTATCGCTC CAGAGTGAAG
     4861 TTCAGCAGGA GCGCAGACGC CCCCGCGTAC CAGCAGGGCC AGAACCAGCT CTATAACGAG
     4921 CTCAATCTAG GACGAAGAGA GGAGTACGAT GTTTTGGACA AGAGACGTGG CCGGGACCCT
     4981 GAGATGGGGG GAAAGCCGAG AAGGAAGAAC CCTCAGGAAG GCCTGTACAA TGAACTGCAG
     5041 AAAGATAAGA TGGCGGAGGC CTACAGTGAG ATTGGGATGA AAGGCGAGCG CCGGAGGGGC
     5101 AAGGGGCACG ATGGCCTTTA CCAGGGTCTC AGTACAGCCA CCAAGGACAC CTACGACGCC
     5161 CTTCACATGC AGGCCCTGCC CCCTCGCCGG GCCAAGCGGT CCGGATCCGG AGCCACCAAC
     5221 TTCAGCCTGC TGAAGCAGGC CGGCGACGTG GAGGAGAACC CCGGCCCCGC GGTGTACCAG
     5281 CTGAGAGACT CTAAATCCAG TGACAAGTCT GTCTGCCTAT TCACCGATTT TGATTCTCAA
     5341 ACAAATGTGT CACAAAGTAA GGATTCTGAT GTGTATATCA CAGACAAAAC TGTGCTAGAC
     5401 ATGAGGTCTA TGGACTTCAA GAGCAACAGT GCTGTGGCCT GGAGCAACAA ATCTGACTTT
     5461 GCATGTGCAA ACGCCTTCAA CAACAGCATT ATTCCAGAAG ACACCTTCTT CCCCAGCCCA
     5521 GGTAAGGGCA GCTTTGGTGC CTTCGCAGGC TGTTTCCTTG CTTCAGGAAT GGCCAGGGGA
     5581 TCCTCTAGAT CGACCTGCAG GCATGCAAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT
     5641 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT
     5701 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC
     5761 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG
     5821 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG
     5881 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA
     5941 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
     6001 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
     6061 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
     6121 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
     6181 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT
     6241 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
     6301 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
     6361 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
     6421 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT
     6481 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
     6541 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
     6601 AAAAAAGGAT CTCAA
//