LOCUS       pEditA                 10489 bp ds-DNA     circular     01-FEB-2023
DEFINITION  .
ACCESSION   JX560339
VERSION     JX560339.2
KEYWORDS    "Responsible person/group" "Usage" "Status" "Parent plasmids"
            "Cloning sites" "Antibiotic resistance" "Origin of replication"
            "Promoters" "RBS" "Genes" "Tags" "Markers" "Spacers" "gRNAs"
            "Terminators" "DNA fragments" "Protein" "Backbones" "Design entry"
            "LabCollector ID" "Comment"
SOURCE      Cloning vector pSEVA441
  ORGANISM  Cloning vector pSEVA441 other sequences; artificial sequences;
            vectors.
COMMENT     Imported using the Genbank importer. File name: sequence.gb-1.txt
            Cloning vector pSEVA441, complete sequence. On Sep 30, 2014 this
            sequence version replaced gi:410073710. ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40 Sequencing Technology ::
            Sanger dideoxy sequencing ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(344..1186)
                     /label="rep gene"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(344..1186)
                     /label="rep CDS"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1602..1706
                     /label="T1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     gene            complement(1726..2691)
                     /label="xylS gene"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(1726..2691)
                     /label="xylS CDS"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3632..3684
                     /label="Pm"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     gene            3731..4327
                     /label="Abe8m-opt"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3880..3880
                     /label="t replaced to cure BsaI"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             4328..8431
                     /label="Cas9(D10A)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(8470..8493)
                     /label="F24"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    8500..8602
                     /label="T0"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        8620..8686
                     /label="promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    8706..8711
                     /label="BsaI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(8706..8725)
                     /label="BsaI-spc CDS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    8719..8725
                     /label="BsaI"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    8726..8801
                     /label="gRNA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             8938..9843
                     /label="aadA CDS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     gene            8938..9843
                     /label="aadA gene"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    9854..10099
                     /label="oriT"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
       61 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      121 ggagaaaggc ggacaggcat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      181 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      241 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      301 acgcggccgt gaaaggcagg ccggtccgtg gtggccacgg cctctaggcc agatccagcg
      361 gcatctgggt tagtcgagcg cgggccgctt cccatgtctc accagggcga gcctgtttcg
      421 cgatctcagc atctgaaatc ttcccggcct tgcgcttcgc tggggcctta cccaccgcct
      481 tggcgggctt cttcggtcca aaactgaaca acagatgtgt gaccttgcgc ccggtctttc
      541 gctgcgccca ctccacctgt agcgggctgt gctcgttgat ctgcgtcacg gctggatcaa
      601 gcactcgcaa cttgaagtcc ttgatcgagg gataccggcc ttccagttga aaccactttc
      661 gcagctggtc aatttctatt tcgcgctggc cgatgctgtc ccattgcatg agcagctcgt
      721 aaagcctgat cgcgtgggtg ctgtccatct tggccacgtc agccaaggcg tatttggtga
      781 actgtttggt gagttccgtc aggtacggca gcatgtcttt ggtgaacctg agttctacac
      841 ggccctcacc ctcccggtag atgattgttt gcacccagcc ggtaatcatc acactcggtc
      901 ttttcccctt gccattgggc tcttgggtta accggacttc ccgccgtttc aggcgcaggg
      961 ccgcttcttt gagctggttg taggaagatt cgatagggac acccgccatc gtcgctatgt
     1021 cctccgccgt cactgaatac atcacttcat cggtgacagg ctcgctcctc ttcacctggc
     1081 taatacaggc cagaacgatc cgctgttcct gaacactgag gcgatacgcg gcctcgacca
     1141 gggcattgct tttgtaaacc attgggggtg aggccacgtt cgacattcct tgtgtataag
     1201 gggacactgt atctgcgtcc cacaatacaa caaatccgtc cctttacaac aacaaatccg
     1261 tcccttctta acaacaaatc cgtcccttaa tggcaacaaa tccgtccctt tttaaactct
     1321 acaggccacg gattacgtgg cctgtagacg tcctaaaagg tttaaaaggg aaaaggaaga
     1381 aaagggtgga aacgcaaaaa acgcaccact acgtggcccc gttggggccg catttgtgcc
     1441 cctgaagggg cgggggaggc gtctgggcaa tccccgtttt accagtcccc tatcgccgcc
     1501 tgagagggcg caggaagcga gtaatcaggg tatcgaggcg gattcaccct tggcgtccaa
     1561 ccagcggcac cagcggcgcc tgagaggggc gcgcccagct gtctagggcg gcggatttgt
     1621 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
     1681 ctgagccttt cgttttattt gatgccttta attaaacgtt cgtaatcaag ccacttcctt
     1741 tttgcattga cgcagggtgt cggaaggcaa ctcgccgaac gcgctcctat agttttcagc
     1801 gaagcgtccc aaatgtaaga agccgtagtc tagggctatc tcagttatac tacgcacatt
     1861 ggcactggga tcgttcaagc aggcgcggat gctttcgagc ttgcggttgc ggatgtagtt
     1921 cttcggcgtg gtgccggcgt gcttctcgaa caaattgtag agcgagcgtg gactcatcat
     1981 cgccagctcc gctaaccgct caaggctgat attccgtttg agattctcct caatgaattg
     2041 aacgactcgc tcgaaagacg ggttaccttt gctgaaaatt tcacggctga cattgctgcc
     2101 cagcatttcg agcagcttgg aagcgatgat ccccgcatag tgctcttgga cccgaggcat
     2161 cgactttgta tgttccgctt cgtcacaaac taacccgagt agattgataa agccatcgag
     2221 ttgctggaga ttgtgtcgcg cggcgaaacg gataccctcc ctcggcttgt gccaattgtt
     2281 gtcactgcac gcccgatcaa ggaccactga gggcaattta acgataaatt tctcgcaatc
     2341 ttctgaatag gtcaggtcgg cttggtcatc cggattgagc agcaatagtt cgcccggcgc
     2401 aaaatagtgc tcctggccat ggccacgcca caggcaatgg cctttgagta ttatttgcag
     2461 atgataacag gtttctaatc caggcgagat taccctcacg ctaccgccgt agctgattcg
     2521 acacagatcg aggcatccga agattctgtg gtgcagcctg cctgccgggc gcccgccctt
     2581 gggcaggcga atagagtgcg taccgacata ctggttaaca taatcggaga ctgcataggg
     2641 ctcggcgtgg acgaagatct gacttttctc gttcaataag caaaaatcca tagttcacgg
     2701 ttctcttatt ttaatgtggg ctgcttggtg tgatgtagaa aggcgccaag tcgatgaaaa
     2761 tgcatctcga cgtgatgcgt atacgggtta cccccattgc cacgttgcgc catccttttt
     2821 gcaatcagtg accacttttc caagcaaaaa taacgccaag cagaacgaag acgttctttt
     2881 taagaagcga gaacaccaga agttcgtgct gtcggggcat ggggcgacga attggcggat
     2941 aaaggggatc tgctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     3001 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc gtaattacgt
     3061 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     3121 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     3181 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     3241 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     3301 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgcat
     3361 gggaattagc ttgatctgac caacgaccgg tagcggagct atccaacggc ggtataccag
     3421 gaaaacacac agcaggtaca tcagaacagt accatgactg aagaacaaat agttttttcc
     3481 tgatccataa agcagaacgg cctgctccat gacaaatctg gctccccaac taatgcccca
     3541 tgcagccagc ataaccagca taaagtgcag tgtccggttt gatagggata agtccagcct
     3601 tgcaagaagc ggatacagga gtgcaaaaaa tggctatctc tagtaaggcc taccccttag
     3661 gctttatgca acagaaacaa taataatgga gtcatgacca tgcctaggcc gcggTTAGGA
     3721 GGAAAAACAT atgagcgaag tcgagttctc ccatgagtat tggatgcggc acgctctgac
     3781 cctcgcgaaa cgtgctcgcg acgagcggga agtcccagtg ggcgctgtcc tcgtgctgaa
     3841 taaccgcgtg attggcgaag gctggaatcg ggccatcggA ctccatgatc ctacggcaca
     3901 cgcagaaatc atggccctgc gccagggtgg cctcgtcatg caaaattatc ggctgtacga
     3961 cgccacgttg tattccacct tcgagccctg cgtcatgtgt gccggggcaa tgattcactc
     4021 ccgtattggg cgtgtggtgt tcggggtgcg caatgcaaag accggcgctg ccggctcgct
     4081 catggatgtc ttgcaccatc ccggtatgaa ccaccgggtc gagattaccg aaggcatttt
     4141 ggccgacgag tgcgccgcgc tcttgtgccg gttttttcgt atgccacggc gtgtgttcaa
     4201 cgctcaaaag aaagcacaaa gctccaccga ttccgggggt tcctcgggcg gctcgagcgg
     4261 gtccgaaacc cctggtacgt cggaatcggc tacgccagaa tcgtccggcg gctcgtccgg
     4321 cggttctgat aagaaatact caataggctt agctatcggc acaaatagcg tcggatgggc
     4381 ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga
     4441 ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc
     4501 ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat
     4561 ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca
     4621 tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt
     4681 tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg
     4741 aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc
     4801 gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag
     4861 tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa
     4921 ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc
     4981 aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg
     5041 gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc
     5101 agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt
     5161 ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc
     5221 tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc
     5281 ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt
     5341 tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata
     5401 tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat
     5461 tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct
     5521 gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact tgggtgagct
     5581 gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa
     5641 gattgaaaaa atcttgactt ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa
     5701 tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga
     5761 agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga
     5821 taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac
     5881 ggtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt
     5941 tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt
     6001 aaccgttaag caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga
     6061 aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa
     6121 aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat
     6181 tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata
     6241 tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg
     6301 gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat
     6361 attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga
     6421 tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag
     6481 tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca
     6541 gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat
     6601 cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga
     6661 gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca
     6721 tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg
     6781 aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga
     6841 tcacattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg
     6901 ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat
     6961 gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa
     7021 tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg
     7081 ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat
     7141 gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa
     7201 atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa
     7261 caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa
     7321 gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg
     7381 taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta
     7441 ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa
     7501 acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga
     7561 ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga
     7621 agtacagaca ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct
     7681 tattgctcgt aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt
     7741 agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc
     7801 cgttaaagag ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat
     7861 tgacttttta gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc
     7921 taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga
     7981 attacaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc
     8041 tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt
     8101 ggagcagcat aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg
     8161 tgttatttta gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga
     8221 caaaccaata cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg
     8281 agctcccgct gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac
     8341 aaaagaagtt ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg
     8401 cattgatttg agtcagctag gaggtgactg atctagagtc gacctgcagg catgcaagct
     8461 tgcggccgcg tcgtgactgg gaaaaccctg gcgactagtc ttggactcct gttgatagat
     8521 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
     8581 ttttttattg gtgagaatcc aggggtccct ttaattaaTT GTTGACAATT AATCATCGGC
     8641 ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCCCTA GGccgcggcc
     8701 gcgcggagac ccgagactgg tctcagtttt agagctagaa atagcaagtt aaaataaggc
     8761 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg caattcgagc tcggtacccg
     8821 gggatcccaa taattacgat ttacgtattt aaatgaacct tgaccgaacg cagcggtggt
     8881 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     8941 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     9001 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa
     9061 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     9121 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     9181 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     9241 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     9301 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     9361 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     9421 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     9481 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     9541 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     9601 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     9661 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     9721 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     9781 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     9841 taagacaatt gtccttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt
     9901 cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact
     9961 ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga
    10021 gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg
    10081 ctctgcgagg ctggccgtag gccggccgat aatctcatga ccaaaatccc ttaacgtgag
    10141 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10201 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10261 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10321 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10381 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10441 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggata
//