LOCUS       pTCas9_scr              8171 bp ds-DNA     circular     11-AUG-2023
DEFINITION  .
ACCESSION   pACYC184
SOURCE      Unknown.
  ORGANISM  Unknown Unclassified.
COMMENT     Imported using the Genbank importer. File name: pACYC184_empty
            vector.gbk SECID/File created by Clone Manager, Scientific &
            Educational Software SECNOTES|GenBank               4245 bp    DNA
            circular SYN 01-MAR-2018 ACCESSION   pACYC184 KEYWORDS:   .
FEATURES             Location/Qualifiers
     misc_feature    complement(126..785)
                     /label="Cat CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1414..1959
                     /label="p15a ori misc_feature"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(1992..2046)
                     /label="Terminator"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(2047..5295)
                     /label="ThermoCas9"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(2047..5295)
                     /label="Translation 5295-2047"
                     /translation="MKYKIGLDIGITSIGWAVINLDIPRIEDLGVRIFDRAENPKTGESLALPRRLARSARRRLRRRKHRLERIRRLFVREGILTKEELNKLFEKKHEIDVWQLRVEALDRKLNNDELARILLHLAKRRGFRSNRKSERTNKENSTMLKHIEENQSILSSYRTVAEMVVKDPKFSLHKRNKEDNYTNTVARDDLEREIKLIFAKQREYGNIVCTEAFEHEYISIWASQRPFASKDDIEKKVGFCTFEPKEKRAPKATYTFQSFTVWEHINKLRLVSPGGIRALTDDERRLIYKQAFHKNKITFHDVRTLLNLPDDTRFKGLLYDRNTTLKENEKVRFLELGAYHKIRKAIDSVYGKGAAKSFRPIDFDTFGYALTMFKDDTDIRSYLRNEYEQNGKRMENLADKVYDEELIEELLNLSFSKFGHLSLKALRNILPYMEQGEVYSTACERAGYTFTGPKKKQKTVLLPNIPPIANPVVMRALTQARKVVNAIIKKYGSPVSIHIELARELSQSFDERRKMQKEQEGNRKKNETAIRQLVEYGLTLNPTGLDIVKFKLWSEQNGKCAYSLQPIEIERLLEPGYTEVDHVIPYSRSLDDSYTNKVLVLTKENREKGNRTPAEYLGLGSERWQQFETFVLTNKQFSKKKRDRLLRLHYDENEENEFKNRNLNDTRYISRFLANFIREHLKFADSDDKQKVYTVNGRITAHLRSRWNFNKNREESNLHHAVDAAIVACTTPSDIARVTAFYQRREQNKELSKKTDPQFPQPWPHFADELQARLSKNPKESIKALNLGNYDNEKLESLQPVFVSRMPKRSITGAAHQETLRRYIGIDERSGKIQTVVKKKLSEIQLDKTGHFPMYGKESDPRTYEAIRQRLLEHNNDPKKAFQEPLYKPKKNGELGPIIRTIKIIDTTNQVIPLNDGKTVAYNSNIVRVDVFEKDGKYYCVPIYTIDMMKGILPNKAIEPNKPYSEWKEMTEDYTFRFSLYPNDLIRIEFPREKTIKTAVGEEIKIKDLFAYYQTIDSSNGGLSLVSHDNNFSLRSIGSRTLKRFEKYQVDVLGNIYKVRGEKRVGVASSSHSKAGETIRPL*"
     misc_feature    complement(5296..5336)
                     /label="5' UTR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    5337..5361
                     /label="lac operator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(5362..5396)
                     /label="Ptet"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(5368..5396)
                     /label="-35 to -10"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        5784..5861
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             5862..6944
                     /label="lacI"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(5881..5900)
                     /label="LacI-R"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(6965..6986)
                     /label="Terminator"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    7005..7006
                     /label="Additiona nt necessary for J23119"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    7007..7039
                     /label="J23119 promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    7040..7062
                     /label="Spacer scr"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    7063..7226
                     /label="ThermoCas9 sgRNA module"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    7227..7263
                     /label="rho-independent terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg
       61 cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa
      121 aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
      181 ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc
      241 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
      301 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
      361 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
      421 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
      481 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
      541 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga
      601 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
      661 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
      721 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
      781 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
      841 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt
      901 cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct
      961 gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc
     1021 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat
     1081 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
     1141 acatcagcgc tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt
     1201 gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat
     1261 acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
     1321 gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta
     1381 acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa
     1441 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
     1501 ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac
     1561 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
     1621 ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct
     1681 gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac
     1741 tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa
     1801 ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc
     1861 aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt
     1921 tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag
     1981 ataaaatatt tGTCGACAGG ATTAAACAAA AATGGCCCGC TTCATAAGCA GGCCATTTTG
     2041 TCTGATTTAT AACGGACGGA TAGTTTCCCC GGCTTTCGAA TGAGAAGATG ACGCCACCCC
     2101 AACTCTCTTT TCCCCTCTCA CTTTGTAGAT GTTGCCTAGC ACATCTACTT GGTATTTCTC
     2161 GAATCGTTTG AGGGTTCTTG AACCGATGCT GCGGAGCGAA AAGTTGTTAT CATGGCTAAC
     2221 CAAACTTAAC CCTCCATTGG AGGAGTCGAT GGTTTGATAA TAGGCGAACA GATCCTTAAT
     2281 TTTGATTTCT TCCCCCACAG CAGTCTTTAT TGTTTTTTCT CGGGGAAATT CGATACGGAT
     2341 AAGATCATTT GGGTATAGAC TGAATCGGAA TGTATAGTCC TCCGTCATTT CCTTCCACTC
     2401 AGAGTACGGT TTGTTCGGCT CGATCGCCTT GTTTGGCAAG ATCCCTTTCA TCATATCTAT
     2461 TGTATAGATA GGGACACAAT AATATTTGCC ATCTTTCTCA AAGACGTCGA CCCGCACGAT
     2521 GTTGCTGTTG TAGGCGACTG TTTTGCCATC GTTGAGCGGA ATAACTTGAT TTGTCGTATC
     2581 GATGATTTTG ATTGTTCGGA TGATAGGACC TAGTTCTCCG TTCTTCTTCG GTTTATACAG
     2641 AGGCTCTTGA AACGCCTTTT TTGGGTCATT GTTATGTTCA AGCAACCGTT GGCGAATGGC
     2701 TTCATATGTC CTTGGATCGC TTTCTTTCCC GTACATTGGG AAATGACCTG TTTTATCCAG
     2761 TTGGATCTCG GATAGTTTCT TTTTGACGAC CGTCTGTATT TTTCCGCTCC GTTCGTCGAT
     2821 GCCGATATAA CGCCGCAATG TTTCTTGATG AGCCGCTCCT GTTATGCTCC GCTTCGGCAT
     2881 TCGGGAGACA AAAACCGGCT GCAACGATTC GAGTTTCTCG TTATCATAAT TTCCAAGATT
     2941 GAGAGCTTTT ATACTCTCCT TTGGATTTTT TGATAAACGC GCCTGCAGTT CATCAGCAAA
     3001 GTGCGGCCAA GGCTGCGGAA ACTGCGGATC CGTCTTTTTG GACAGTTCTT TGTTTTGTTC
     3061 GCGCCGTTGA TAGAAGGCGG TGACTCGGGC GATATCGCTC GGCGTTGTGC AGGCGACGAT
     3121 GGCAGCATCG ACGGCATGAT GCAAATTCGA TTCTTCCCGG TTTTTGTTAA AATTCCAACG
     3181 GCTGCGTAAA TGGGCGGTAA TACGGCCGTT GACCGTGTAT ACTTTTTGTT TGTCATCGCT
     3241 GTCGGCGAAT TTGAGATGTT CGCGAATAAA GTTAGCCAAG AAGCGTGAGA TATAACGGGT
     3301 ATCATTTAGA TTACGATTTT TAAACTCATT TTCTTCGTTT TCATCGTAAT GAAGCCGAAG
     3361 GAGTCGATCC CGCTTCTTTT TCGAAAACTG CTTATTTGTC AAGACAAACG TCTCGAACTG
     3421 TTGCCAACGT TCTGAGCCTA ATCCTAAATA TTCAGCTGGG GTGCGGTTTC CTTTTTCACG
     3481 GTTCTCCTTT GTCAACACAA GAACTTTATT GGTATAGCTA TCGTCCAAGC TTCGGCTGTA
     3541 TGGAATCACA TGGTCGACTT CTGTATAGCC TGGTTCGAGC AACCGCTCGA TTTCGATCGG
     3601 TTGGAGTGAA TAGGCACATT TTCCGTTTTG TTCGCTCCAT AGTTTGAATT TCACAATGTC
     3661 AAGCCCAGTT GGATTGAGCG TCAGCCCATA TTCAACAAGT TGGCGAATGG CAGTTTCGTT
     3721 TTTCTTTCGG TTTCCTTCCT GTTCTTTCTG CATTTTACGT CGTTCATCAA AGGATTGTGA
     3781 TAGTTCCCGG GCCAGTTCGA TATGGATGGA GACCGGTGAG CCGTACTTTT TGATAATGGC
     3841 ATTGACCACT TTGCGTGCCT GTGTCAGTGC GCGCATGACG ACCGGATTGG CGATCGGCGG
     3901 AATGTTCGGC AGCAATACCG TTTTCTGTTT TTTCTTTGGC CCTGTAAATG TATATCCTGC
     3961 TCGTTCACAA GCGGTTGAGT AGACTTCGCC TTGTTCCATA TATGGAAGGA TGTTGCGAAG
     4021 CGCTTTAAGG GATAGATGAC CAAACTTAGA AAACGATAAG TTTAAAAGTT CTTCAATCAA
     4081 TTCTTCATCA TAGACTTTAT CCGCTAGATT TTCCATTCGT TTTCCATTTT GTTCGTATTC
     4141 GTTTCGCAAG TAACTGCGAA TGTCGGTGTC GTCTTTAAAC ATCGTTAATG CGTAGCCAAA
     4201 TGTATCAAAA TCAATCGGAC GAAATGATTT TGCTGCTCCT TTGCCATAGA CGCTGTCGAT
     4261 CGCTTTCCGT ATTTTATGAT AGGCGCCGAG TTCAAGGAAG CGAACTTTCT CATTTTCCTT
     4321 CAGCGTGGTG TTTCGGTCAT ATAAAAGACC TTTAAAACGG GTGTCGTCAG GCAAGTTAAG
     4381 CAATGTTCGA ACATCATGGA AGGTGATTTT ATTTTTATGA AATGCTTGCT TGTATATAAG
     4441 ACGACGTTCA TCATCGGTTA GTGCCCGGAT GCCTCCCGGG GAGACAAGAC GAAGTTTGTT
     4501 AATATGTTCC CAGACGGTGA AGGACTGGAA TGTGTATGTT GCTTTTGGCG CGCGTTTTTC
     4561 TTTAGGCTCA AACGTACAGA AACCGACTTT TTTCTCGATA TCATCCTTAG AAGCAAAAGG
     4621 GCGTTGCGAT GCCCAAATGG AAATATACTC GTGTTCAAAT GCTTCTGTGC AAACGATGTT
     4681 CCCATATTCG CGCTGTTTGG CGAAAATCAG TTTGATTTCC CGTTCAAGAT CGTCGCGGGC
     4741 AACAGTGTTG GTGTAATTAT CCTCTTTATT ACGCTTGTGC AGGGAAAATT TCGGATCCTT
     4801 GACAACCATT TCTGCAACCG TTCGGTAACT TGAAAGAATG GATTGGTTTT CTTCAATATG
     4861 TTTGAGCATC GTACTGTTTT CTTTGTTGGT GCGCTCACTC TTGCGGTTGG ATCTAAATCC
     4921 ACGCCGTTTA GCCAGATGAA GAAGGATGCG GGCTAATTCA TCGTTATTTA GTTTTCGATC
     4981 CAGTGCTTCA ACACGAAGCT GCCAGACGTC GATTTCGTGC TTTTTTTCAA ACAGCTTGTT
     5041 CAGCTCTTCC TTCGTTAAAA TTCCTTCGCG GACGAACAGG CGGCGAATGC GCTCCAGTCG
     5101 ATGTTTGCGA CGCCGCAGAC GACGTCGGGC GGAGCGGGCG AGGCGACGTG GAAGAGCTAG
     5161 TGACTCCCCG GTTTTCGGGT TTTCCGCTCT GTCAAAAATG CGGACACCTA AATCTTCGAT
     5221 GCGAGGAATG TCCAAATTAA TGACAGCCCA ACCGATAGAC GTAATGCCGA TATCAAGACC
     5281 GATTTTATAC TTCATacacg gtgcctgact gcgttagcaa tttaactgtg ataaacggaa
     5341 ttgttatccg ctcacaattc ctaccgcatt aaagcttatc gatgataagc tgtcaaacat
     5401 gagaattaca acttatatcg tatggggctg acttcaggtg ctacatttga agagataaat
     5461 tgcactgaaa tctagatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt
     5521 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     5581 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     5641 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
     5701 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     5761 taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg
     5821 catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat
     5881 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg
     5941 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt
     6001 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg
     6061 ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg
     6121 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct
     6181 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc
     6241 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat
     6301 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta
     6361 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg
     6421 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc
     6481 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc
     6541 aacaaaccat gcaaatgctg aatgagggta tcgttcccac tgcgatgctg gttgccaacg
     6601 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg
     6661 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa
     6721 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac
     6781 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa
     6841 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6901 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgaTCAGAA TCGACTGATG
     6961 GACCAAAAAA CCCCGCCGAA GCGGGGACTT TGAACTAGCA TACATTGACA GCTAGCTCAG
     7021 TCCTAGGTAT AATGCTAGCC TAGATCCGCA GTAACCCCAT GGGTCATAGT TCCCCTGAGA
     7081 TTATCGCTGT GGTATAATGA AAGTTATACC ACAGCAATGA TCTCAGGGTT ACTATGATAA
     7141 GGGCTTTCTG CCTATAGGCA GACTGACCCG TGGCGTTGGG GATCGCCTAT CGCCCGCTTT
     7201 CTTCGGGCAT TCCCCACTCT TAGGCGAACG AAGACCTTGG CGGAAAACGT CAAGGTCTTT
     7261 TTTatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc
     7321 aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt
     7381 ccgccatctc cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg
     7441 tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg
     7501 gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt
     7561 ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa
     7621 cgcggaagtc ccctacgtgc tgctgaagtt gcccgcaaca gagagtggaa ccaaccggtg
     7681 ataccacgat actatgactg agagtcaacg ccatgagcgg cctcatttct tattctgagt
     7741 tacaacagtc cgcaccgctg tccggtagct ccttccggtg ggcgcggggc atgactatcg
     7801 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     7861 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgccctgca
     7921 ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat
     7981 ctgtattaac gaagcgctaa ccgtttttat caggctctgg gaggcagaat aaatgatcat
     8041 atcgtcaatt attacctcca cggggagagc ctgagcaaac tggcctcagg catttgagaa
     8101 gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc
     8161 ggctatttaa c
//