LOCUS       pAcr                    2770 bp ds-DNA     circular     11-AUG-2023
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name: pUK19.gbk pUC
            cloning vector.
FEATURES             Location/Qualifiers
     rep_origin      121..709
                     /label="ori"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     610..629
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        997..1115
                     /label="rhaB promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    997..1175
                     /label="PrhaBAD + RBS"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     RBS             1149..1171
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1176..1178
                     /label="Extra nt"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1179..1436
                     /label="AcrIIC1 (codon optimized for E. coli)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1467..1514
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        1755..1859
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             1860..2720
                     /label="AmpR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(2078..2097)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
       61 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
      121 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
      181 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      241 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      301 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      361 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      421 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      481 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      541 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      601 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      661 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      721 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      781 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      841 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      901 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      961 tcccgactgg aaagcgggca gtgagcgcaa cgcaatCACC ACAATTCAGC AAATTGTGAA
     1021 CATCATCACG TTCATCTTTC CCTGGTTGCC AATGGCCCAT TTTCCTGTCA GTAACGAGAA
     1081 GGTCGCGAAT TCAGGCGCTT TTTAGACTGG TCGTAGGGAG ACCACAACGG TTTCCCTCTA
     1141 GAAATAATTT TGTTTAACTA TAAGAAGGAG ATATACATAT GAATAAAACA TATAAAATCG
     1201 GAAAAAATGC GGGATATGAC GGCTGCGGGT TATGTTTAGC CGCGATCTCA GAAAATGAAG
     1261 CTATTAAAGT TAAGTATCTG CGCGATATCT GTCCAGACTA TGACGGCGAC GACAAAGCTG
     1321 AGGACTGGCT GAGATGGGGA ACGGATAGCC GCGTGAAAGC TGCGGCTTTA GAAATGGAGC
     1381 AATATGCTTA TACGTCGGTT GGGATGGCGT CATGTTGGGA GTTTGTTGAA CTATGACCTA
     1441 GAGCGGCCGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT
     1501 TGAGGGGTTT TTTGttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     1561 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     1621 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     1681 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     1741 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     1801 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1861 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     1921 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1981 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     2041 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     2101 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     2161 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     2221 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     2281 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     2341 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     2401 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     2461 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     2521 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     2581 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     2641 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     2701 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     2761 taaaacttca
//