LOCUS       pAcrTCas9_scr           8611 bp ds-DNA     circular     11-AUG-2023
DEFINITION  .
ACCESSION   pACYC184
SOURCE      Unknown.
  ORGANISM  Unknown Unclassified.
COMMENT     Imported using the Genbank importer. File name: pACYC184_empty
            vector.gbk SECID/File created by Clone Manager, Scientific &
            Educational Software SECNOTES|GenBank               4245 bp    DNA
            circular SYN 01-MAR-2018 ACCESSION   pACYC184 KEYWORDS:   .
FEATURES             Location/Qualifiers
     misc_feature    complement(126..785)
                     /label="Cat CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1414..1959
                     /label="p15a ori misc_feature"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        1992..2110
                     /label="rhaB promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1992..2170
                     /label="PrhaBAD + RBS"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     RBS             2144..2166
                     /label="RBS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2171..2173
                     /label="Extra nt"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2174..2431
                     /label="AcrIIC1 (codon optimized for E. coli)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(2432..2486)
                     /label="Terminator"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(2487..5735)
                     /label="ThermoCas9"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(2487..5735)
                     /label="Translation 5735-2487"
                     /translation="MKYKIGLDIGITSIGWAVINLDIPRIEDLGVRIFDRAENPKTGESLALPRRLARSARRRLRRRKHRLERIRRLFVREGILTKEELNKLFEKKHEIDVWQLRVEALDRKLNNDELARILLHLAKRRGFRSNRKSERTNKENSTMLKHIEENQSILSSYRTVAEMVVKDPKFSLHKRNKEDNYTNTVARDDLEREIKLIFAKQREYGNIVCTEAFEHEYISIWASQRPFASKDDIEKKVGFCTFEPKEKRAPKATYTFQSFTVWEHINKLRLVSPGGIRALTDDERRLIYKQAFHKNKITFHDVRTLLNLPDDTRFKGLLYDRNTTLKENEKVRFLELGAYHKIRKAIDSVYGKGAAKSFRPIDFDTFGYALTMFKDDTDIRSYLRNEYEQNGKRMENLADKVYDEELIEELLNLSFSKFGHLSLKALRNILPYMEQGEVYSTACERAGYTFTGPKKKQKTVLLPNIPPIANPVVMRALTQARKVVNAIIKKYGSPVSIHIELARELSQSFDERRKMQKEQEGNRKKNETAIRQLVEYGLTLNPTGLDIVKFKLWSEQNGKCAYSLQPIEIERLLEPGYTEVDHVIPYSRSLDDSYTNKVLVLTKENREKGNRTPAEYLGLGSERWQQFETFVLTNKQFSKKKRDRLLRLHYDENEENEFKNRNLNDTRYISRFLANFIREHLKFADSDDKQKVYTVNGRITAHLRSRWNFNKNREESNLHHAVDAAIVACTTPSDIARVTAFYQRREQNKELSKKTDPQFPQPWPHFADELQARLSKNPKESIKALNLGNYDNEKLESLQPVFVSRMPKRSITGAAHQETLRRYIGIDERSGKIQTVVKKKLSEIQLDKTGHFPMYGKESDPRTYEAIRQRLLEHNNDPKKAFQEPLYKPKKNGELGPIIRTIKIIDTTNQVIPLNDGKTVAYNSNIVRVDVFEKDGKYYCVPIYTIDMMKGILPNKAIEPNKPYSEWKEMTEDYTFRFSLYPNDLIRIEFPREKTIKTAVGEEIKIKDLFAYYQTIDSSNGGLSLVSHDNNFSLRSIGSRTLKRFEKYQVDVLGNIYKVRGEKRVGVASSSHSKAGETIRPL*"
     misc_feature    complement(5736..5776)
                     /label="5' UTR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    5777..5801
                     /label="lac operator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(5802..5836)
                     /label="Ptet"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(5808..5836)
                     /label="-35 to -10"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        6224..6301
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             6302..7384
                     /label="lacI"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(6321..6340)
                     /label="LacI-R"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(7405..7426)
                     /label="Terminator"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    7445..7446
                     /label="Additiona nt necessary for J23119"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    7447..7479
                     /label="J23119 promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    7480..7502
                     /label="Spacer scr"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    7503..7666
                     /label="ThermoCas9 sgRNA module"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    7667..7703
                     /label="rho-independent terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg
       61 cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa
      121 aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
      181 ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc
      241 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
      301 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
      361 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
      421 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
      481 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
      541 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga
      601 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
      661 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
      721 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
      781 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
      841 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt
      901 cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct
      961 gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc
     1021 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat
     1081 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
     1141 acatcagcgc tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt
     1201 gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat
     1261 acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
     1321 gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta
     1381 acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa
     1441 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
     1501 ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac
     1561 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
     1621 ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct
     1681 gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac
     1741 tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa
     1801 ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc
     1861 aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt
     1921 tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag
     1981 ataaaatatt tCACCACAAT TCAGCAAATT GTGAACATCA TCACGTTCAT CTTTCCCTGG
     2041 TTGCCAATGG CCCATTTTCC TGTCAGTAAC GAGAAGGTCG CGAATTCAGG CGCTTTTTAG
     2101 ACTGGTCGTA GGGAGACCAC AACGGTTTCC CTCTAGAAAT AATTTTGTTT AACTATAAGA
     2161 AGGAGATATA CATATGAATA AAACATATAA AATCGGAAAA AATGCGGGAT ATGACGGCTG
     2221 CGGGTTATGT TTAGCCGCGA TCTCAGAAAA TGAAGCTATT AAAGTTAAGT ATCTGCGCGA
     2281 TATCTGTCCA GACTATGACG GCGACGACAA AGCTGAGGAC TGGCTGAGAT GGGGAACGGA
     2341 TAGCCGCGTG AAAGCTGCGG CTTTAGAAAT GGAGCAATAT GCTTATACGT CGGTTGGGAT
     2401 GGCGTCATGT TGGGAGTTTG TTGAACTATG AGTCGACAGG ATTAAACAAA AATGGCCCGC
     2461 TTCATAAGCA GGCCATTTTG TCTGATTTAT AACGGACGGA TAGTTTCCCC GGCTTTCGAA
     2521 TGAGAAGATG ACGCCACCCC AACTCTCTTT TCCCCTCTCA CTTTGTAGAT GTTGCCTAGC
     2581 ACATCTACTT GGTATTTCTC GAATCGTTTG AGGGTTCTTG AACCGATGCT GCGGAGCGAA
     2641 AAGTTGTTAT CATGGCTAAC CAAACTTAAC CCTCCATTGG AGGAGTCGAT GGTTTGATAA
     2701 TAGGCGAACA GATCCTTAAT TTTGATTTCT TCCCCCACAG CAGTCTTTAT TGTTTTTTCT
     2761 CGGGGAAATT CGATACGGAT AAGATCATTT GGGTATAGAC TGAATCGGAA TGTATAGTCC
     2821 TCCGTCATTT CCTTCCACTC AGAGTACGGT TTGTTCGGCT CGATCGCCTT GTTTGGCAAG
     2881 ATCCCTTTCA TCATATCTAT TGTATAGATA GGGACACAAT AATATTTGCC ATCTTTCTCA
     2941 AAGACGTCGA CCCGCACGAT GTTGCTGTTG TAGGCGACTG TTTTGCCATC GTTGAGCGGA
     3001 ATAACTTGAT TTGTCGTATC GATGATTTTG ATTGTTCGGA TGATAGGACC TAGTTCTCCG
     3061 TTCTTCTTCG GTTTATACAG AGGCTCTTGA AACGCCTTTT TTGGGTCATT GTTATGTTCA
     3121 AGCAACCGTT GGCGAATGGC TTCATATGTC CTTGGATCGC TTTCTTTCCC GTACATTGGG
     3181 AAATGACCTG TTTTATCCAG TTGGATCTCG GATAGTTTCT TTTTGACGAC CGTCTGTATT
     3241 TTTCCGCTCC GTTCGTCGAT GCCGATATAA CGCCGCAATG TTTCTTGATG AGCCGCTCCT
     3301 GTTATGCTCC GCTTCGGCAT TCGGGAGACA AAAACCGGCT GCAACGATTC GAGTTTCTCG
     3361 TTATCATAAT TTCCAAGATT GAGAGCTTTT ATACTCTCCT TTGGATTTTT TGATAAACGC
     3421 GCCTGCAGTT CATCAGCAAA GTGCGGCCAA GGCTGCGGAA ACTGCGGATC CGTCTTTTTG
     3481 GACAGTTCTT TGTTTTGTTC GCGCCGTTGA TAGAAGGCGG TGACTCGGGC GATATCGCTC
     3541 GGCGTTGTGC AGGCGACGAT GGCAGCATCG ACGGCATGAT GCAAATTCGA TTCTTCCCGG
     3601 TTTTTGTTAA AATTCCAACG GCTGCGTAAA TGGGCGGTAA TACGGCCGTT GACCGTGTAT
     3661 ACTTTTTGTT TGTCATCGCT GTCGGCGAAT TTGAGATGTT CGCGAATAAA GTTAGCCAAG
     3721 AAGCGTGAGA TATAACGGGT ATCATTTAGA TTACGATTTT TAAACTCATT TTCTTCGTTT
     3781 TCATCGTAAT GAAGCCGAAG GAGTCGATCC CGCTTCTTTT TCGAAAACTG CTTATTTGTC
     3841 AAGACAAACG TCTCGAACTG TTGCCAACGT TCTGAGCCTA ATCCTAAATA TTCAGCTGGG
     3901 GTGCGGTTTC CTTTTTCACG GTTCTCCTTT GTCAACACAA GAACTTTATT GGTATAGCTA
     3961 TCGTCCAAGC TTCGGCTGTA TGGAATCACA TGGTCGACTT CTGTATAGCC TGGTTCGAGC
     4021 AACCGCTCGA TTTCGATCGG TTGGAGTGAA TAGGCACATT TTCCGTTTTG TTCGCTCCAT
     4081 AGTTTGAATT TCACAATGTC AAGCCCAGTT GGATTGAGCG TCAGCCCATA TTCAACAAGT
     4141 TGGCGAATGG CAGTTTCGTT TTTCTTTCGG TTTCCTTCCT GTTCTTTCTG CATTTTACGT
     4201 CGTTCATCAA AGGATTGTGA TAGTTCCCGG GCCAGTTCGA TATGGATGGA GACCGGTGAG
     4261 CCGTACTTTT TGATAATGGC ATTGACCACT TTGCGTGCCT GTGTCAGTGC GCGCATGACG
     4321 ACCGGATTGG CGATCGGCGG AATGTTCGGC AGCAATACCG TTTTCTGTTT TTTCTTTGGC
     4381 CCTGTAAATG TATATCCTGC TCGTTCACAA GCGGTTGAGT AGACTTCGCC TTGTTCCATA
     4441 TATGGAAGGA TGTTGCGAAG CGCTTTAAGG GATAGATGAC CAAACTTAGA AAACGATAAG
     4501 TTTAAAAGTT CTTCAATCAA TTCTTCATCA TAGACTTTAT CCGCTAGATT TTCCATTCGT
     4561 TTTCCATTTT GTTCGTATTC GTTTCGCAAG TAACTGCGAA TGTCGGTGTC GTCTTTAAAC
     4621 ATCGTTAATG CGTAGCCAAA TGTATCAAAA TCAATCGGAC GAAATGATTT TGCTGCTCCT
     4681 TTGCCATAGA CGCTGTCGAT CGCTTTCCGT ATTTTATGAT AGGCGCCGAG TTCAAGGAAG
     4741 CGAACTTTCT CATTTTCCTT CAGCGTGGTG TTTCGGTCAT ATAAAAGACC TTTAAAACGG
     4801 GTGTCGTCAG GCAAGTTAAG CAATGTTCGA ACATCATGGA AGGTGATTTT ATTTTTATGA
     4861 AATGCTTGCT TGTATATAAG ACGACGTTCA TCATCGGTTA GTGCCCGGAT GCCTCCCGGG
     4921 GAGACAAGAC GAAGTTTGTT AATATGTTCC CAGACGGTGA AGGACTGGAA TGTGTATGTT
     4981 GCTTTTGGCG CGCGTTTTTC TTTAGGCTCA AACGTACAGA AACCGACTTT TTTCTCGATA
     5041 TCATCCTTAG AAGCAAAAGG GCGTTGCGAT GCCCAAATGG AAATATACTC GTGTTCAAAT
     5101 GCTTCTGTGC AAACGATGTT CCCATATTCG CGCTGTTTGG CGAAAATCAG TTTGATTTCC
     5161 CGTTCAAGAT CGTCGCGGGC AACAGTGTTG GTGTAATTAT CCTCTTTATT ACGCTTGTGC
     5221 AGGGAAAATT TCGGATCCTT GACAACCATT TCTGCAACCG TTCGGTAACT TGAAAGAATG
     5281 GATTGGTTTT CTTCAATATG TTTGAGCATC GTACTGTTTT CTTTGTTGGT GCGCTCACTC
     5341 TTGCGGTTGG ATCTAAATCC ACGCCGTTTA GCCAGATGAA GAAGGATGCG GGCTAATTCA
     5401 TCGTTATTTA GTTTTCGATC CAGTGCTTCA ACACGAAGCT GCCAGACGTC GATTTCGTGC
     5461 TTTTTTTCAA ACAGCTTGTT CAGCTCTTCC TTCGTTAAAA TTCCTTCGCG GACGAACAGG
     5521 CGGCGAATGC GCTCCAGTCG ATGTTTGCGA CGCCGCAGAC GACGTCGGGC GGAGCGGGCG
     5581 AGGCGACGTG GAAGAGCTAG TGACTCCCCG GTTTTCGGGT TTTCCGCTCT GTCAAAAATG
     5641 CGGACACCTA AATCTTCGAT GCGAGGAATG TCCAAATTAA TGACAGCCCA ACCGATAGAC
     5701 GTAATGCCGA TATCAAGACC GATTTTATAC TTCATacacg gtgcctgact gcgttagcaa
     5761 tttaactgtg ataaacggaa ttgttatccg ctcacaattc ctaccgcatt aaagcttatc
     5821 gatgataagc tgtcaaacat gagaattaca acttatatcg tatggggctg acttcaggtg
     5881 ctacatttga agagataaat tgcactgaaa tctagatttc gcgggatcga gatctcgatc
     5941 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     6001 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     6061 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     6121 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     6181 ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg
     6241 caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa
     6301 tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
     6361 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
     6421 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
     6481 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
     6541 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
     6601 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
     6661 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
     6721 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
     6781 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
     6841 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
     6901 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
     6961 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggta tcgttcccac
     7021 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
     7081 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc
     7141 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
     7201 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
     7261 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     7321 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7381 gtgaTCAGAA TCGACTGATG GACCAAAAAA CCCCGCCGAA GCGGGGACTT TGAACTAGCA
     7441 TACATTGACA GCTAGCTCAG TCCTAGGTAT AATGCTAGCC TAGATCCGCA GTAACCCCAT
     7501 GGGTCATAGT TCCCCTGAGA TTATCGCTGT GGTATAATGA AAGTTATACC ACAGCAATGA
     7561 TCTCAGGGTT ACTATGATAA GGGCTTTCTG CCTATAGGCA GACTGACCCG TGGCGTTGGG
     7621 GATCGCCTAT CGCCCGCTTT CTTCGGGCAT TCCCCACTCT TAGGCGAACG AAGACCTTGG
     7681 CGGAAAACGT CAAGGTCTTT TTTatggaag ccggcggcac ctcgctaacg gattcaccac
     7741 tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg
     7801 gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag
     7861 cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg
     7921 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag
     7981 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg
     8041 tgtttcgtaa agtctggaaa cgcggaagtc ccctacgtgc tgctgaagtt gcccgcaaca
     8101 gagagtggaa ccaaccggtg ataccacgat actatgactg agagtcaacg ccatgagcgg
     8161 cctcatttct tattctgagt tacaacagtc cgcaccgctg tccggtagct ccttccggtg
     8221 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     8281 aggacaggtg ccggcagcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     8341 gccgaaacaa gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct
     8401 accctgtgga acacctacat ctgtattaac gaagcgctaa ccgtttttat caggctctgg
     8461 gaggcagaat aaatgatcat atcgtcaatt attacctcca cggggagagc ctgagcaaac
     8521 tggcctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta
     8581 aaccagcaat agacataagc ggctatttaa c
//