LOCUS       pKA3        7131 bp DNA     circular SYN 08-OCT-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Jens
  TITLE     Direct Submission
  JOURNAL   Exported Aug 11, 2023 from SnapGene Viewer 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7131
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      102..557
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        584..688
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             689..1549
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  689 ..  757 / #ccffcc / signal sequence
                        2:  758 .. 1549 / #ccffcc
                     Cleavage site after base 758"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1720..2308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
                     /note="color: #ffe4c4"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /note="color: #ccffcc"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(3624..3652)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
                     /note="This reverse directional feature has 3 segments:
                        1: 3624 .. 3629 / #ffffff / -10
                        2: 3630 .. 3646 / #ffffff
                        3: 3647 .. 3652 / #ffffff / -35"
     polyA_signal    4161..4209
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
                     /note="color: #a6acb3"
     promoter        complement(4345..4454)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
                     /note="color: #ffffff; direction: LEFT"
     promoter        4478..4569
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     5282..5300
                     /label=Primer 2
                     /note="color: black; sequence: ggatccGGTGTGGAAAGTC; added: 
                     2019-10-08"
     misc_feature    5282..5287
                     /label=BamHI
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    5288..5556
                     /label=SV40 promoter
                     /note="color: #ff80ff; direction: RIGHT"
     primer_bind     5360..5372
                     /label=Primer 2
                     /note="color: black; sequence: ggatccGGTGTGGAAAGTC; added: 
                     2019-10-08"
     misc_feature    5611..6210
                     /label=Puro
                     /note="color: #0080ff; direction: RIGHT"
     misc_feature    6211..6259
                     /label=Synthetic poly(A)
                     /note="color: #ffafb8; direction: RIGHT"
     primer_bind     complement(6242..6265)
                     /label=Primer 1
                     /note="color: black; sequence: AAGCTTCACACAAAAAACCAACAC; 
                     added: 2019-10-08"
     misc_feature    6260..6265
                     /label=HindIII
                     /note="color: #00ff00"
     polyA_signal    6721..6855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     mobile_element  6884..7049
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
                     /note="color: #ffe4c4"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcct acgtacccgt agtggctatg gcagggcttg ccgccccgac
     4021 gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga
     4081 aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg
     4141 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
     4201 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
     4261 tcccccatct cccggtaccg catgctatgc atcagctgct agcaccatgg ctcgagatcc
     4321 cgggtgatca agtcttcgtc gagtgattgt aaataaaatg taatttacag tatagtattt
     4381 taattaatat acaaatgatt tgataataat tcttatttaa ctataatata ttgtgttggg
     4441 ttgaattaaa ggtccgtata ctccggaata ttaatagatc atggagataa ttaaaatgat
     4501 aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa
     4561 cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc ccggtccgaa
     4621 gcgcgcggaa ttcaaaggcc tacgtcgacg agctcactag tcgcggccgc tttcgaatct
     4681 agagcctgca gtctcgacaa gcttgtcgag aagtactaga ggatcataat cagccatacc
     4741 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     4801 cataaaatga atgcaattgt tgttgttgga actcggacgc atcccactga gccgagacaa
     4861 gattctgctg tagtcagtgc tgcctgggaa tctattttca caaagttctc caaaaaatgt
     4921 gatgatcaaa actaggaatt agtgttctgt gtcttaggcc ctaaaatctt cctgtgaatt
     4981 ccatttttaa ggtagtcgag gtgaaccgcg tctggtctgc agaggataga aaaaaggccc
     5041 tctgatacct caagttagtt tcacctttaa agaaggtcgg aagtaaagac gcaaagcctt
     5101 tcccggacgt gcggaagggc aacgtccttc ctcatggccg gaaatggaac tttaatttcc
     5161 cgttcccccc aaccagcccg cccgagagag tgactctcac gagagccgcg agagtcagct
     5221 tggccaatcc gtgcggtcgg cggccgctcc ctttataagc cgactcgccc ggcagcgcac
     5281 cggatccggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5341 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5401 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5461 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5521 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     5581 tttggaggcc taggcttttg caaaaagctc atgaccgagt acaagcccac ggtgcgcctc
     5641 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     5701 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     5761 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     5821 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     5881 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     5941 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6001 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6061 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6121 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6181 tggtgcatga cccgcaagcc cggtgcctga aataaaatat ctttattttc attacatctg
     6241 tgtgttggtt ttttgtgtga agcttagttc gctttcctgt tggtgggggg aacgccgatc
     6301 gtgcgcatcc gtcacccctc gccggcaatg ggggcttgtg aacccccaaa cctgactgac
     6361 tgggccagtg tgctgcaaat tggcaggaga cgtgaaggca cctccaaagt cggccaaaat
     6421 gaatgggcag tgagccgggg ttgcctggag ccgttcctgc gtgggttctc ccgtcttccg
     6481 ctttttgttg ccttttatgg ttgtattaca acttagttcc tgctctgcag attttgttga
     6541 ggtttttgct tctcccaagg tagatctcga ccagtcccct caacggggtg tgggagaaca
     6601 gtcatttttt tttgagagat catttaacat ttaatgaata tttaattaga agatctaaat
     6661 gaacattgga aattgtgttc ctttaatggt catcggttta tgccagaggt tagaagtttc
     6721 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6781 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6841 tatcatgtct ggatctgatc actgcttgag cctaggagat ccgaaccaga taagtgaaat
     6901 ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag
     6961 ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc
     7021 ccagttccca actattttgt ccgcccacag cggggcattt ttcttcctgt tatgttttta
     7081 atcaaacatc ctgccaactc catgtgacaa accgtcatct tcggctactt t
//
