LOCUS       pCS2_Opto_Alk8          5784 bp    DNA        circular     13-AUG-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1783..1788
                     /locus_tag="EcoRI"
                     /label="EcoRI"
                     /ApEinfo_label="EcoRI"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#cccccc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    79..84
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#cccccc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    301..325
                     /locus_tag="GS domain"
                     /label="GS domain"
                     /ApEinfo_label="GS domain"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1804..1809
                     /locus_tag="XbaI"
                     /label="XbaI"
                     /ApEinfo_label="XbaI"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#cccccc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    364..1239
                     /locus_tag="Alk8 serine/threonine kinase"
                     /label="Alk8 serine/threonine kinase"
                     /ApEinfo_label="Alk8 serine/threonine kinase"
                     /ApEinfo_fwdcolor="#00ff80"
                     /ApEinfo_revcolor="#00ff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1796..1801
                     /locus_tag="XhoI"
                     /label="XhoI"
                     /ApEinfo_label="XhoI"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#cccccc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1790..1795
                     /locus_tag="StuI"
                     /label="StuI"
                     /ApEinfo_label="StuI"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#cccccc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2080..2100
                     /locus_tag="T3 promoter"
                     /label="T3 promoter"
                     /ApEinfo_label="T3 promoter"
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    35..53
                     /locus_tag="Sp6 promoter"
                     /label="Sp6 promoter"
                     /ApEinfo_label="Sp6 promoter"
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1811..1828
                     /locus_tag="T7 promoter"
                     /label="T7 promoter"
                     /ApEinfo_label="T7 promoter"
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1306..1740
                     /vntifkey="Unknown"
                     /locus_tag="VfLOV domain"
                     /label="VfLOV domain"
                     /ApEinfo_label="VfLOV domain"
                     /ApEinfo_fwdcolor="#75d5ff"
                     /ApEinfo_revcolor="#75d5ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    127..171
                     /vntifkey="21"
                     /locus_tag="GS\15aa\linker"
                     /label="GS\15aa\linker"
                     /ApEinfo_label="GS\15aa\linker"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1261..1305
                     /vntifkey="21"
                     /locus_tag="GS\15aalinker"
                     /label="GS\15aalinker"
                     /ApEinfo_label="GS\15aalinker"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1753..1779
                     /vntifkey="21"
                     /locus_tag="HA"
                     /label="HA"
                     /ApEinfo_label="HA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    85..126
                     /locus_tag="Myristoylation domain"
                     /label="Myristoylation domain"
                     /ApEinfo_label="Myristoylation domain"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="#ff8040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2034..2041
                     /locus_tag="NotI"
                     /label="NotI"
                     /ApEinfo_label="NotI"
                     /ApEinfo_fwdcolor="#cd8aff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
       61 CTACTTGTTC TTTTTGCAGG ATCCatgggg agtagcaaga gcaagcctaa ggaccccagc
      121 cagcgcggtg gaggaggttc tggaggcggt ggaagtggtg gcggaggtag cCGTCGACTC
      181 CATCATGGGC GTCTGGAGAG ACTGCACGAG TTTGACACTG AACAGGGGGC CATCGATGGG
      241 CTTATCGCGT CTAATGTCGG AGACAGCACA CTTGCGGATC TGATGGATCA CTCCTGCACT
      301 TCAGGCAGTG GTTCAGGACT GCCCTTCCTG GTTCAGAGAA CGGTTGCGCG GCAGATCAGC
      361 CTGGTGGAGT GTGTTGGTAA AGGACGGTAC GGTGAAGTGT GGAGAGGTCA ATGGCAAGGA
      421 GAAAATGTAG CCGTGAAGAT CTTTTCCTCT AGAGATGAGA AGTCATGGTT TCGAGAAACA
      481 GAAATTTACA ACACTGTTCT GCTACGACAT GAAAATATAT TAGGCTTCAT GGCTTCTGAC
      541 ATGACCTCCC GAAACTCTAG CACTCAGCTG TGGCTGATCA CACACTATCA CGAGAATGGC
      601 TCTCTGTATG ACTACCTGCA GCGTGTGGCT GTGGAGATGG CAGATGGACT GCACATGGCG
      661 GCTTCGATTG CCAGCGGGCT GGTGCACCTG CACACGGAGA TCTTTGGCAC GGAGGGCAAA
      721 CCGGCCATCG CTCACAGAGA CCTGAAGAGC AAGAACATCC TTGTGAAGAA AGATTTGCAG
      781 TGCTGCATCG CTGACCTGGG TCTGGCAGTA ACACACACGC AGTCTGATAA TCAGCTTGAT
      841 GTGGGAAATA ATCCTAAAGT GGGAACCAAA CGCTACATGG CACCGGAGGT TCTAGATGAG
      901 ACCATTCAGA CGGACTGTTT TGACGCCTAT AAGAGGGTGG ATATCTGGGC CTTTGGGTTG
      961 GTGCTGTGGG AGATCGCACG CAGAACCATC AGCAATGGAA TTGTAGAGGA ATACAAGCCG
     1021 CCTTTCTATG ACCTGGTTCC TAATGATCCC AGCTTTGACG ACATGAGGAA AGTGGTTTGT
     1081 GTGGAGCAGC AAAGGCCATT CATTCCCAAC CGCTGGTTTT CAGATCCTAC CCTGTCTGCT
     1141 CTGGTGAAGC TGATGAAAGA GTGCTGGTAC CAGAACCCCT CGGCTCGTCT CACTGCCCTG
     1201 CGCATCAAAA AGACTCTGGA TAAAATCCAC AGTTCACTGG AGAAGGGCAA AACCGACTGC
     1261 ggtggaggag gttctggagg cggtggaagt ggtggcggag gtagccctga ctacagtctc
     1321 gtgaaggctc tgcaaatggc acaacagaat tttgtcatta cagacgcctc cctcccagac
     1381 aaccctatcg tctacgccag tagagggttt ctgacactga caggctattc tctcgaccag
     1441 atcctgggca ggaactgcag gtttctgcaa gggccagaaa cagacccaag agctgtggat
     1501 aagatcagga atgccatcac caaaggcgtt gataccagtg tctgtctgct gaattataga
     1561 caggatggca caaccttctg gaatctcttc ttcgtggctg gactcagaga ttctaagggc
     1621 aatattgtca actacgtcgg agtgcagtca aaggtgagcg aagattatgc caagctgctg
     1681 gtcaacgagc agaacattga gtacaaaggt gtgcgcacca gtaacatgct gcgcagaaag
     1741 cccgggtcta gttatccgta cgacgtacca gactacgcat aaGAATTCAA GGCCTCTCGA
     1801 GCCTCTAGAA CTATAGTGAG TCGTATTACG TAGATCCAGA CATGATAAGA TACATTGATG
     1861 AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG
     1921 ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT
     1981 GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA TTCGCGGCCG
     2041 CGGCGCCAAT GCATTGGGCC CGGTACCCAG CTTTTGTTCC CTTTAGTGAG GGTTAATTGC
     2101 GCGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT
     2161 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG
     2221 CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG
     2281 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC
     2341 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC
     2401 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA
     2461 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT
     2521 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     2581 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     2641 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     2701 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     2761 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     2821 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     2881 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     2941 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     3001 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     3061 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
     3121 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     3181 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     3241 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
     3301 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
     3361 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     3421 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     3481 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     3541 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
     3601 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     3661 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     3721 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     3781 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     3841 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
     3901 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     3961 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     4021 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     4081 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     4141 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     4201 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
     4261 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA
     4321 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA
     4381 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC
     4441 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA
     4501 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT
     4561 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA
     4621 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC
     4681 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC
     4741 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGTCG
     4801 ACCATAGCCA ATTCAATATG GCGTATATGG ACTCATGCCA ATTCAATATG GTGGATCTGG
     4861 ACCTGTGCCA ATTCAATATG GCGTATATGG ACTCGTGCCA ATTCAATATG GTGGATCTGG
     4921 ACCCCAGCCA ATTCAATATG GCGGACTTGG CACCATGCCA ATTCAATATG GCGGACTTGG
     4981 CACTGTGCCA ACTGGGGAGG GGTCTACTTG GCACGGTGCC AAGTTTGAGG AGGGGTCTTG
     5041 GCCCTGTGCC AAGTCCGCCA TATTGAATTG GCATGGTGCC AATAATGGCG GCCATATTGG
     5101 CTATATGCCA GGATCAATAT ATAGGCAATA TCCAATATGG CCCTATGCCA ATATGGCTAT
     5161 TGGCCAGGTT CAATACTATG TATTGGCCCT ATGCCATATA GTATTCCATA TATGGGTTTT
     5221 CCTATTGACG TAGATAGCCC CTCCCAATGG GCGGTCCCAT ATACCATATA TGGGGCTTCC
     5281 TAATACCGCC CATAGCCACT CCCCCATTGA CGTCAATGGT CTCTATATAT GGTCTTTCCT
     5341 ATTGACGTCA TATGGGCGGT CCTATTGACG TATATGGCGC CTCCCCCATT GACGTCAATT
     5401 ACGGTAAATG GCCCGCCTGG CTCAATGCCC ATTGACGTCA ATAGGACCAC CCACCATTGA
     5461 CGTCAATGGG ATGGCTCATT GCCCATTCAT ATCCGTTCTC ACGCCCCCTA TTGACGTCAA
     5521 TGACGGTAAA TGGCCCACTT GGCAGTACAT CAATATCTAT TAATAGTAAC TTGGCAAGTA
     5581 CATTACTATT GGAAGGACGC CAGGGTACAT TGGCAGTACT CCCATTGACG TCAATGGCGG
     5641 TAAATGGCCC GCGATGGCTG CCAAGTACAT CCCCATTGAC GTCAATGGGG AGGGGCAATG
     5701 ACGCAAATGG GCGTTCCATT GACGTAAATG GGCGGTAGGC GTGCCTAATG GGAGGTCTAT
     5761 ATAAGCAATG CTCGTTTAGG GAAC   
//
