LOCUS       pEVOL-NBK-1             7008 bp ds-DNA     circular     02-AUG-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     Promoter              7..291
                           /label="araBAD promoter"
                           /ApEinfo_revcolor="#c6c9d1"
                           /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind           296..316
                           /label="T7"
                           /ApEinfo_revcolor="#85dae9"
                           /ApEinfo_fwdcolor="#85dae9"
     Ribosome binding      318..340
                           /label="RBS"
                           /ApEinfo_revcolor="#9eafd2"
                           /ApEinfo_fwdcolor="#9eafd2"
     protein               354..1718
                           /label="NBK1"
                           /ApEinfo_revcolor="#faac61"
                           /ApEinfo_fwdcolor="#faac61"
     CDS                   354..1718
                           /label="Translation 354-1718"
                           /translation="MDKKPLNTLISATGLWMSRTGTIHKIKHHEVSRSKIYIEMACGDHLVVNNSRSSRTARALRHHKYRKTCKRCRVSDEDLNKFLTKANEDQTSVKVKVVSAPTRTKKAMPKSVARAPKPLENTEAAQAQPSGSKFSPAIPVSTQESVSVPASVSTSISSISTGATASALVKGNTNPITSMSAPVQASAPALTKSQTDRLEVLLNPKDEISLNSGKPFRELESELLSRRKKDLQQIYAEERENYLGKLEREITRFFVDRGFLEIKSPILIPLEYIERMGIDNDTELSKQIFRVDKNFCLRPMLAPNLMNYARKLDRALPDPIKIFEIGPCYRKESDGKEHLEEFTMLNFAQMGSGCTRENLESIITDFLNHLGIDFKIVGDSCMVFGDTLDVMHGDLELSSAVVGPIPLDREWGIDKPWIGAGFGLERLLKVKHDFKNIKRAARSESYYNGISTNL*"
     terminator            1935..1989
                           /label="rrnb t1"
                           /ApEinfo_revcolor="#84b0dc"
                           /ApEinfo_fwdcolor="#84b0dc"
     protein               2119..3483
                           /label="NBK1"
                           /ApEinfo_revcolor="#faac61"
                           /ApEinfo_fwdcolor="#faac61"
     misc_feature          3906..3976
                           /label="tRNA_CUA"
                           /ApEinfo_revcolor="#ff9ccd"
                           /ApEinfo_fwdcolor="#ff9ccd"
     CDS                   complement(4136..4792)
                           /label="CmR"
                           /ApEinfo_revcolor="#ffef86"
                           /ApEinfo_fwdcolor="#ffef86"
     promoter              complement(4793..4895)
                           /label="cat promoter"
                           /ApEinfo_revcolor="#c6c9d1"
                           /ApEinfo_fwdcolor="#c6c9d1"
     Origin of Replication complement(5421..5966)
                           /label="p15A ori"
                           /ApEinfo_revcolor="#ffef86"
                           /ApEinfo_fwdcolor="#ffef86"
     Regulatory Protein    complement(6112..6990)
                           /label="araC"
                           /ApEinfo_revcolor="#d6b295"
                           /ApEinfo_fwdcolor="#d6b295"
ORIGIN
        1 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact
       61 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg
      121 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc
      181 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
      241 tagcggatcc tacctgacgc TTTTTATCGC AACTCTCTAC TGTTTCTCCA TACCCGTAAT
      301 ACGACTCACT ATAGGGTTTT GTTTAACTTT AAGAAGGAGA TATACTTACC CATATGGATA
      361 AAAAGCCTCT GAACACTCTG ATTTCTGCGA CCGGTCTGTG GATGTCCCGC ACCGGCACCA
      421 TCCACAAAAT CAAACACCAT GAAGTTAGCC GTTCCAAAAT CTACATTGAA ATGGCTTGCG
      481 GCGATCACCT GGTTGTCAAC AACTCCCGTT CTTCTCGTAC CGCTCGCGCA CTGCGCCACC
      541 ACAAATATCG CAAAACCTGC AAACGTTGCC GTGTTAGCGA TGAAGATCTG AACAAATTCC
      601 TGACCAAAGC TAACGAGGAT CAGACCTCCG TAAAAGTGAA GGTAGTAAGC GCTCCGACCC
      661 GTACTAAAAA GGCTATGCCA AAAAGCGTGG CCCGTGCCCC GAAACCTCTG GAAAACACCG
      721 AGGCGGCTCA GGCTCAACCA TCCGGTTCTA AATTTTCTCC GGCGATCCCA GTGTCCACCC
      781 AAGAATCTGT TTCCGTACCA GCAAGCGTGT CTACCAGCAT TAGCAGCATT TCTACCGGTG
      841 CTACCGCTTC TGCGCTGGTA AAAGGTAACA CTAACCCGAT TACTAGCATG TCTGCACCGG
      901 TACAGGCAAG CGCCCCAGCT CTGACTAAAT CCCAGACGGA CCGTCTGGAG GTGCTGCTGA
      961 ACCCAAAGGA TGAAATCTCT CTGAACAGCG GCAAGCCTTT CCGTGAGCTG GAAAGCGAGC
     1021 TGCTGTCTCG TCGTAAAAAG GATCTGCAAC AGATCTACGC TGAGGAACGC GAGAACTATC
     1081 TGGGTAAGCT GGAGCGCGAA ATTACTCGCT TCTTCGTGGA TCGCGGTTTC ctggagatca
     1141 aatctccgat tctgattccg ctggaATACA TTGAACGTAT GGGCATCGAT AATGATACCG
     1201 AACTGTCTAA ACAGATCTTC CGTGTGGATA AAAACTTCTG TCTGCGTCCG ATGCTGGCCC
     1261 CGAACCTGAT GAACTATGCG CGTAAACTGG ACCGTGCCCT GCCGGACCCG ATCAAAATTT
     1321 TCGAGATCGG TCCTTGCTAC CGTAAAGAGT CCGACGGTAA AGAGCACCTG GAAGAATTCA
     1381 CCATGCTGAA CTTCGCGCAG ATGGGTAGCG GTTGCACGCG TGAAAACCTG GAATCCATTA
     1441 TCACCGACTT CCTGAATCAC CTGGGTATCG ATTTCAAAAT TGTTGGTGAC AGCTGTATGG
     1501 TGTTTGGCGA TACGCTGGAT GTTATGCACG GCGATCTGGA GCTGTCTTCC GCAGTAGTGG
     1561 GCCCAATCCC GCTGGATCGT GAGTGGGGTA TCGACAAACC TTGGATCGGT GCGGGTTTTG
     1621 GTCTGGAGCG TCTGCTGAAA GTAAAACACG ACTTCAAGAA CATCAAACGT GCTGCACGTT
     1681 CCGAGTCCTA TTACAATGGT ATTTCTACTA ACCTGTAAgt ctccagcttg gctgttttgg
     1741 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
     1801 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
     1861 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
     1921 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1981 gttgtttgtg agctcccggt catcaatcat ccccataatc cttgttagat tatcaatttt
     2041 aaaaaactaa cagttgtcag cctgtcccgc tttaatatca tacgccgtta tacgttgttt
     2101 acgctttgag gaatcccaAT GGATAAAAAG CCTCTGAACA CTCTGATTTC TGCGACCGGT
     2161 CTGTGGATGT CCCGCACCGG CACCATCCAC AAAATCAAAC ACCATGAAGT TAGCCGTTCC
     2221 AAAATCTACA TTGAAATGGC TTGCGGCGAT CACCTGGTTG TCAACAACTC CCGTTCTTCT
     2281 CGTACCGCTC GCGCACTGCG CCACCACAAA TATCGCAAAA CCTGCAAACG TTGCCGTGTT
     2341 AGCGATGAAG ATCTGAACAA ATTCCTGACC AAAGCTAACG AGGATCAGAC CTCCGTAAAA
     2401 GTGAAGGTAG TAAGCGCTCC GACCCGTACT AAAAAGGCTA TGCCAAAAAG CGTGGCCCGT
     2461 GCCCCGAAAC CTCTGGAAAA CACCGAGGCG GCTCAGGCTC AACCATCCGG TTCTAAATTT
     2521 TCTCCGGCGA TCCCAGTGTC CACCCAAGAA TCTGTTTCCG TACCAGCAAG CGTGTCTACC
     2581 AGCATTAGCA GCATTTCTAC CGGTGCTACC GCTTCTGCGC TGGTAAAAGG TAACACTAAC
     2641 CCGATTACTA GCATGTCTGC ACCGGTACAG GCAAGCGCCC CAGCTCTGAC TAAATCCCAG
     2701 ACGGACCGTC TGGAGGTGCT GCTGAACCCA AAGGATGAAA TCTCTCTGAA CAGCGGCAAG
     2761 CCTTTCCGTG AGCTGGAAAG CGAGCTGCTG TCTCGTCGTA AAAAGGATCT GCAACAGATC
     2821 TACGCTGAGG AACGCGAGAA CTATCTGGGT AAGCTGGAGC GCGAAATTAC TCGCTTCTTC
     2881 GTGGATCGCG GTTTCctgga gatcaaatct ccgattctga ttccgctgga ATACATTGAA
     2941 CGTATGGGCA TCGATAATGA TACCGAACTG TCTAAACAGA TCTTCCGTGT GGATAAAAAC
     3001 TTCTGTCTGC GTCCGATGCT GGCCCCGAAC CTGATGAACT ATGCGCGTAA ACTGGACCGT
     3061 GCCCTGCCGG ACCCGATCAA AATTTTCGAG ATCGGTCCTT GCTACCGTAA AGAGTCCGAC
     3121 GGTAAAGAGC ACCTGGAAGA ATTCACCATG CTGAACTTCG CGCAGATGGG TAGCGGTTGC
     3181 ACGCGTGAAA ACCTGGAATC CATTATCACC GACTTCCTGA ATCACCTGGG TATCGATTTC
     3241 AAAATTGTTG GTGACAGCTG TATGGTGTTT GGCGATACGC TGGATGTTAT GCACGGCGAT
     3301 CTGGAGCTGT CTTCCGCAGT AGTGGGCCCA ATCCCGCTGG ATCGTGAGTG GGGTATCGAC
     3361 AAACCTTGGA TCGGTGCGGG TTTTGGTCTG GAGCGTCTGC TGAAAGTAAA ACACGACTTC
     3421 AAGAACATCA AACGTGCTGC ACGTTCCGAG TCCTATTACA ATGGTATTTC TACTAACCTG
     3481 TAACTGCAGT TTCAAACGCT AAATTGCCTG ATGCGCTACG CTTATCAGGC CTACATGATC
     3541 TCTGCAATAT ATTGAGTTTG CGTGCTTTTG TAGGCCGGAT AAGGCGTTCA CGCCGCATCC
     3601 GGCAAGAAAC AGCAAACAAT CCAAAACGCC GCGTTCAGCG GCGTTTTTTC TGCTTTTCTT
     3661 CGCGAATTAA TTCCGCTTCG CAACATGTGA GCACCGGTTT ATTGACTACC GGAAGCAGTG
     3721 TGACCGTGTG CTTCTCAAAT GCCTGAGGCC AGTTTGCTCA GGCTCTCCCC GTGGAGGTAA
     3781 TAATTGACGA TATGATCAGT GCACGGCTAA CTAAGCGGCC TGCTGACTTT CTCGCCGATC
     3841 AAAAGGCATT TTGCTATTAA GGGATTGACG AGGGCGTATC TGCGCAGTAA GATGCGCCCC
     3901 GCATTTGGCG GAAACCCCGG GAATCTAACC CGGCTGAACG GATTTAGAGT CCATTCGATC
     3961 TACATGATCA GGTTTCCAAT TCGAAAAGCC TGCTCAACGA GCAGGCTTTT TTGCATCTCG
     4021 AGCAGCTCAG GGTCGAATTT GCTTTCGAAT TTCTGCCATT CATCCGCTTA TTATCACTTA
     4081 TTCAGGCGTA GCAACCAGGC GTTTAAGGGC ACCAATAACT GCCTTAAAAA AATTAcgccc
     4141 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     4201 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     4261 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     4321 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
     4381 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     4441 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     4501 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     4561 tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     4621 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     4681 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     4741 gatgccattg ggatatatca ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATTTTAGCTT
     4801 CCTTAGCTCC TGAAAATCTC GATAACTCAA AAAATACGCC CGGTAGTGAT CTTATTTCAT
     4861 TATGGTGAAA GTTGGAACCT CTTACGTGCC GATCAACGTC TCATTTTCGC CAAAAGTTGG
     4921 CCCAGGGCTT CCCGGTATCA ACAGGGACAC CAGGATTTAT TTATTCTGCG AAGTGATCTT
     4981 CCGTCACAGG TATTTATTCG GCGCAAAGTG CGTCGGGTGA TGCTGCCAAC TTACTGATTT
     5041 AGTGTATGAT GGTGTTTTTG AGGTGCTCCA GTGGCTTCTG TTTCTATCAG CTGTCCCTCC
     5101 TGTTCAGCTA CTGACGGGGT GGTGCGTAAC GGCAAAAGCA CCGCCGGACA TCAGCGCTAG
     5161 CGGAGTGTAT ACTGGCTTAC TATGTTGGCA CTGATGAGGG TGTCAGTGAA GTGCTTCATG
     5221 TGGCAGGAGA AAAAAGGCTG CACCGGTGCG TCAGCAGAAT ATGTGATACA GGATATATTC
     5281 CGCTTCCTCG CTCACTGACT CGCTACGCTC GGTCGTTCGA CTGCGGCGAG CGGAAATGGC
     5341 TTACGAACGG GGCGGAGATT TCCTGGAAGA TGCCAGGAAG ATACTTAACA GGGAAGTGAG
     5401 AGGGCCGCGG CAAAGCCGTT TTTCCATAGG CTCCGCCCCC CTGACAAGCA TCACGAAATC
     5461 TGACGCTCAA ATCAGTGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
     5521 CCTGGCGGCT CCCTCGTGCG CTCTCCTGTT CCTGCCTTTC GGTTTACCGG TGTCATTCCG
     5581 CTGTTATGGC CGCGTTTGTC TCATTCCACG CCTGACACTC AGTTCCGGGT AGGCAGTTCG
     5641 CTCCAAGCTG GACTGTATGC ACGAACCCCC CGTTCAGTCC GACCGCTGCG CCTTATCCGG
     5701 TAACTATCGT CTTGAGTCCA ACCCGGAAAG acatgcaaaa gcaccactgg cagcagccac
     5761 tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa
     5821 ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag
     5881 ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga
     5941 ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct
     6001 agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata
     6061 agttgtaatt ctcatgtttg acagcttatc atcgataagc ttggtaccca attatgacaa
     6121 cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc
     6181 cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg
     6241 accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg
     6301 ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag
     6361 acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc
     6421 caggtgatcg ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag
     6481 cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag
     6541 cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct
     6601 gaaatgcggc tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg
     6661 ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca
     6721 ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat
     6781 atcactcggt cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat
     6841 ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata
     6901 accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg
     6961 cagcagggga tcattttgcg cttcagccat actttttact cccgccat
//