LOCUS       pCMV-PE6a        8985 bp DNA     circular UNA 20-AUG-2023
DEFINITION  natural circular DNA
ACCESSION   <unknown
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 21, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8985
                     /mol_type="genomic DNA"
                     /organism="."
     CDS             complement(171..1031)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1032..1136)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(1207..1251)
                     /label=bom
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=32.37410071942446"
                     /note="/other=misc feature"
     enhancer        1406..1785
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        1786..1989
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        2031..2049
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             2063..2119
                     /label=SV40 NLS
                     /note="/created by=smreets"
     CDS             2120..6220
                     /label=Cas9(H840A)
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.927"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     CDS             6221..6322
                     /label=SGGSx2-sv40NLS-SGGSx2
                     /note="/created by=smreets"
     CDS             6221..6277
                     /label=Linker
                     /note="/created by=smreets"
     CDS             6278..6298
                     /label=SV40 NLS
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     CDS             6299..6322
                     /label=Linker
                     /note="/created by=smreets"
     CDS             6323..7543
                     /label=evoEc48
                     /note="/created by=smreets"
     CDS             7556..7609
                     /label=SV40 NLS
                     /note="/created by=smreets"
     CDS             7619..7645
                     /label=c-Myc NLS
                     /note="/created by=smreets"
                     /note="/modified by=smreets"
     polyA_signal    7700..7924
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA signal"
     promoter        7928..7949
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.197183098591549"
                     /note="/other=promoter"
     protein_bind    8017..8039
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=95.83333333333334"
                     /note="/other=protein bind"
     promoter        complement(8043..8073)
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=96.875"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    8088..8109
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein bind"
     CDS             complement(8122..8215)
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.679593721144968"
                     /note="/other=CDS"
     rep_origin      complement(8397..8985)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacactcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     1201 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     1261 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     1321 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     1381 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     1441 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     1501 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1561 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     1621 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     1681 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     1741 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     1801 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     1861 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     1921 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     1981 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact
     2041 cactataggg agagccgcca ccatgaaacg gacagccgac ggaagcgagt tcgagtcacc
     2101 aaagaagaag cggaaagtcg acaagaagta cagcatcggc ctggacatcg gcaccaactc
     2161 tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct
     2221 gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag
     2281 cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg
     2341 gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga
     2401 cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg
     2461 gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat
     2521 ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta
     2581 tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa
     2641 ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct
     2701 gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag
     2761 actgagcaag agcagaaagc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa
     2821 tggcctgttc ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa
     2881 cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct
     2941 ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa
     3001 cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc
     3061 ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct
     3121 gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag
     3181 caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt
     3241 catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaagag
     3301 agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca
     3361 cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga
     3421 caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct
     3481 ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc
     3541 ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat
     3601 gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta
     3661 cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag
     3721 aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac
     3781 caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt
     3841 cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca
     3901 cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat
     3961 tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg
     4021 gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag
     4081 atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc
     4141 cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca
     4201 gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg
     4261 ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa
     4321 gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa
     4381 gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa
     4441 gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat
     4501 cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta
     4561 cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga
     4621 ctacgatgtg gacgctatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa
     4681 ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt
     4741 cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag
     4801 aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg
     4861 cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct
     4921 ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt
     4981 gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt
     5041 gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac
     5101 cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt
     5161 gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa
     5221 gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg
     5281 cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga
     5341 taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt
     5401 gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa
     5461 cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga
     5521 cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa
     5581 gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga
     5641 gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat
     5701 catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc
     5761 ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt
     5821 cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa
     5881 acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga
     5941 gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa
     6001 caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct
     6061 gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag
     6121 gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct
     6181 gtacgagaca cggatcgacc tgtctcagct gggaggtgac tccggcggaa gctctggtgg
     6241 cagcaagcgg accgccgacg gctctgaatt cgagagccct aagaagaaaa gaaaggtgag
     6301 cggaggctct agcggcggaa gcggaagacc ttacgttaca ctgaatctga acggcatgtt
     6361 catggacaag ttcaagccat acagcaaatc taatgcccct attaccaccc tggagaagct
     6421 gagcaaagct ctgtccatca gcgtagagga gctgaaggct atcgccgaac tgagcctgga
     6481 cgagaaatac acactgaaga aaatccccaa gatcgacggc agcaagagga ttgtgtactc
     6541 cctccatcct aagatgcggc tgctgcaaag cagaatcaac gaaagaatct tcaaggaact
     6601 ggtggtgttc ccatcttttc tgttcggctc cgtgcctagc aaaaatgacg tgctgaacag
     6661 caatgtgaag cgggattacg tgtcctgcgc caaggcccac tgcggcgcca aaaccgtact
     6721 gaaagtggac atcagcaact tcttcgacaa catccataga gatctggtca gatctgtgtt
     6781 cgaggaaatc ctgcacatca aagatgaggc cctggattac ctggtcgata tctgcacaaa
     6841 ggacgacttc gtggtccagg gagccctgac aagctcttat atcgccaccc tgtgcctgtt
     6901 tgccgtggaa ggcgacgtgg tgcggcgggc ccagagaaag ggactggtgt acacaagact
     6961 ggtggatgac atcaccgtgt cttctaaaat ctccaactac gacttcagcc agatgcagag
     7021 ccacatcgag cggatgctgt ctgagcacaa cctgcccatc aacaagcaca agaccaagat
     7081 cttccactgt agcagcgagc ctatcaaggt gcacggcctg atagttgact acgacagccc
     7141 cagactgcct tctgataagg tgaagcgcat ccgggccagc atccacaact tgaagctgct
     7201 ggccgccaag aacaacacca agaccagcgt ggcctacaga aaggaattca acagatgcat
     7261 gggcagagtg aacgagctgg gccgggtggg ccacgagaag tacgagagct ttaaaaagca
     7321 gctgcaggcc atcaagccta tgcctagcaa tcgggacgtg gccgtgatcg acgccgctat
     7381 caagagcctt gagctgagct acagcaaggg caatcagaac aagcactggt acaagaggaa
     7441 gtatgatctg accagataca agatgatcat cttgaccaga agcgagagct tcaaagagaa
     7501 gctcgaatgt tttaagtccc ggctggctag cctgaagccc ctgtctggcg gctcaaaaag
     7561 aaccgccgac ggcagcgaat tcgagtctcc caagaagaag aggaaagtcg gctctggccc
     7621 tgccgctaag agagtgaagc tggactaacc ggtcatcatc accatcacca ttgagtttaa
     7681 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     7741 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     7801 aaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     7861 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     7921 atggcttctg aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc
     7981 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8041 cacaacatac gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa
     8101 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8161 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8221 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8281 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8341 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8401 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8461 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8521 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8581 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8641 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8701 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8761 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8821 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8881 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8941 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaa
//
