LOCUS       pCMV-PE6f        9255 bp DNA     circular UNA 20-AUG-2023
DEFINITION  natural circular DNA
ACCESSION   <unknown
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 21, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9255
                     /mol_type="genomic DNA"
                     /organism="."
     CDS             complement(171..1031)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1032..1136)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(1207..1251)
                     /label=bom
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=32.37410071942446"
                     /note="/other=misc feature"
     enhancer        1406..1785
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        1786..1989
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        2031..2049
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             2063..2119
                     /label=SV40 NLS
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     CDS             2120..6220
                     /label=Cas9(H840A) PE6f
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.659"
                     /note="/match_length=100.0"
                     /note="/modified by=smreets"
                     /note="/other=CDS"
     CDS             6221..6322
                     /label=SGGSx2-sv40NLS-SGGSx2
                     /note="/created by=smreets"
     CDS             6323..7813
                     /label=PEmaxRNaseHtrunc
                     /note="/created by=smreets"
     CDS             7826..7879
                     /label=SV40 NLS
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     CDS             7889..7915
                     /label=c-Myc NLS
                     /note="/created by=smreets"
     polyA_signal    7970..8194
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA signal"
     promoter        8198..8219
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.197183098591549"
                     /note="/other=promoter"
     protein_bind    8287..8309
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=95.83333333333334"
                     /note="/other=protein bind"
     promoter        complement(8313..8343)
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=96.875"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    8358..8379
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein bind"
     CDS             complement(8392..8485)
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.679593721144968"
                     /note="/other=CDS"
     rep_origin      complement(8667..9255)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacactcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     1201 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     1261 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     1321 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     1381 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     1441 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     1501 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1561 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     1621 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     1681 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     1741 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     1801 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     1861 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     1921 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     1981 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact
     2041 cactataggg agagccgcca ccatgaaacg gacagccgac ggaagcgagt tcgagtcacc
     2101 aaagaagaag cggaaagtcg acaagaagta cagcatcggc ctggacatcg gcaccaactc
     2161 tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct
     2221 gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag
     2281 cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg
     2341 gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga
     2401 cagcttcttc cggagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg
     2461 gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat
     2521 ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta
     2581 tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa
     2641 ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct
     2701 gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag
     2761 actgagcaag agcagaaagc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa
     2821 tggcctgttc ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa
     2881 cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct
     2941 ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa
     3001 cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc
     3061 ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct
     3121 gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag
     3181 caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt
     3241 catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaagag
     3301 agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca
     3361 cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga
     3421 caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct
     3481 ggccagggga aacagcagat tcgcctggat gaccagaaag agcgagaaga ccatcacccc
     3541 ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat
     3601 gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta
     3661 cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag
     3721 aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac
     3781 caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt
     3841 cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca
     3901 cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat
     3961 tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatgg tggaggaacg
     4021 gctgaaaacc tatgcccacc tgttcgacaa caaagtgatg aagcagctga agcggcggag
     4081 atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc
     4141 cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca
     4201 gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg
     4261 ccagggcgat agcctgtacg agcacattgc caatctggcc ggcagccccg ccattaagaa
     4321 gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa
     4381 gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa
     4441 gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat
     4501 cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta
     4561 cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga
     4621 ctacgatgtg gacgctatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa
     4681 ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt
     4741 cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag
     4801 aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg
     4861 cttcatcgcc agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct
     4921 ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt
     4981 gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt
     5041 gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac
     5101 cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt
     5161 gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa
     5221 gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg
     5281 cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga
     5341 taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt
     5401 gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa
     5461 cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga
     5521 cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa
     5581 gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga
     5641 gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat
     5701 catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc
     5761 ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt
     5821 cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa
     5881 acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga
     5941 gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa
     6001 caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct
     6061 gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag
     6121 gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct
     6181 gtacgagaca cggatcgacc tgtctcagct gggaggtgac tccggcggaa gctctggtgg
     6241 cagcaagcgg accgccgacg gctctgaatt cgagagccct aagaagaaaa gaaaggtgag
     6301 cggaggctct agcggcggaa gcaccctgaa cattgaagac gagtatagac tgcatgaaac
     6361 aagcaaggaa cccgacgtgt ccctgggctc cacctggctg tccgactttc cccaggcctg
     6421 ggccgagaca ggaggaatgg gcctggccgt gcggcaggca cccctgatca tccctctgaa
     6481 ggccacctct acacccgtga gcatcaagca gtaccctatg tctcaggagg ccagactggg
     6541 catcaagcct cacatccaga ggctgctgga ccagggcatc ctggtgccat gccagagccc
     6601 ctggaacaca ccactgctgc ccgtgaagaa gccaggcacc aatgactata gacccgtgca
     6661 ggatctgaga gaggtgaaca agagggtgga ggatatccac cccaccgtgc ccaaccctta
     6721 caatctgctg tccggcctgc ccccttctca ccagtggtat acagtgctgg acctgaagga
     6781 tgccttcttt tgtctgagac tgcaccctac cagccagcca ctgttcgcct ttgagtggag
     6841 ggaccctgag atgggcatct ctggccagct gacctggaca cgcctgcctc agggcttcaa
     6901 gaatagccca acactgttta acgaggccct gcaccgcgac ctggcagatt tccggatcca
     6961 gcacccagat ctgatcctgc tgcagtacgt ggacgatctg ctgctggccg ccaccagcga
     7021 gctggattgc cagcagggaa cacgcgccct gctgcagacc ctgggaaacc tgggatatag
     7081 ggcatccgcc aagaaggccc agatctgtca gaagcaggtg aagtacctgg gctatctgct
     7141 gaaggagggc cagagatggc tgacagaggc caggaaggag acagtgatgg gccagccaac
     7201 acccaagacc ccaagacagc tgagggagtt cctgggcaaa gcaggatttt gcaggctgtt
     7261 catcccagga ttcgcagaga tggcagcacc tctgtaccca ctgaccaagc cgggcaccct
     7321 gtttaattgg ggccctgacc agcagaaggc ctatcaggag atcaagcagg ccctgctgac
     7381 agcaccagcc ctgggcctgc cagacctgac caagcctttc gagctgtttg tggatgagaa
     7441 gcagggctac gccaagggcg tgctgaccca gaagctggga ccatggagac ggcccgtggc
     7501 ctatctgtcc aagaagctgg acccagtggc agcaggatgg ccaccatgcc tgaggatggt
     7561 ggcagcaatc gccgtgctga caaaggatgc cggcaagctg accatgggac agccactggt
     7621 catcctggca ccacacgcag tggaggccct ggtgaagcag cctccagatc gctggctgtc
     7681 taacgcccgg atgacacact accaggccct gctgctggac accgatcgcg tgcagtttgg
     7741 ccctgtggtg gccctgaatc cagccaccct gctgcctctg ccagaggagg gcctgcagca
     7801 caactgtctg gactctggcg gctcaaaaag aaccgccgac ggcagcgaat tcgagtctcc
     7861 caagaagaag aggaaagtcg gctctggccc tgccgctaag agagtgaagc tggactaacc
     7921 ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct
     7981 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     8041 actcccactg tcctttccta ataaaatgag aaaattgcat cgcattgtct gagtaggtgt
     8101 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     8161 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     8221 ggctcgatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     8281 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8341 aaagcctagg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8401 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8461 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8521 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8581 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8641 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8701 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8761 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8821 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8881 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8941 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9001 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9061 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9121 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9181 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9241 aaaaaaggat ctcaa
//
