LOCUS       Exported                6399 bp ds-DNA     circular SYN 22-AUG-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Addgene207868_p0340_GW1_mito4xCox8_mCherry_I158E_Q160A
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   TANTAMA LAB
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Aug 22, 2023 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     sig_peptide     189..574
                     /note="4xCOX8 Mitochondrial Targeting Sequence"
     CDS             606..1314
                     /codon_start=1
                     /note="mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEEKARLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    816..824
                     /note="MYG Chromophore"
     variation       1101..1103
                     /note="I158E"
     variation       1107..1109
                     /note="Q160A"
     misc_feature    1339..1358
                     /note="pFastBacR primer"
     misc_feature    1597..1613
                     /note="M13R primer"
     gene            complement(2765..3601)
                     /note="AmpR"
     misc_feature    3884..3906
                     /note="3GEX primer"
     misc_feature    4245..4268
                     /note="M13F (-47) primer"
     misc_feature    4271..4288
                     /note="M13F (-21) primer"
     promoter        4331..1
                     /note="hCMV IE4 Promoter"
     misc_feature    5371..5391
                     /note="CMV-Forward"
ORIGIN
        1 aagcttcccg gccacagcaa tcgatccatg acacaaccag tgacaagcag tcccccagtg
       61 aaaccgcatc cgcatccgag tccgataccg ataaagattc tgaatcggag tgagtgccgc
      121 gtccgagagc gttccctgtc cacgtccacc atcggcggag caggtgtgcc tgaggcctct
      181 agaccaccat gagcgtgctg acaccattgt tgctcagagg cctcactggt tcagccaggc
      241 ggctgccagt cccaagagct aagatccata gtctgggaga tccaatgtca gttctcactc
      301 cccttttgct taggggactg accggaagcg ctcggcggtt gcctgtccca cgcgccaaga
      361 ttcactccct gggcgaccct atgagtgtcc ttactcctct tttgctgaga ggcttgacag
      421 ggagcgctcg gaggctccca gtgcctcgcg caaagatcca ttctctcgga gatcccatgt
      481 ccgtcctcac tcctctgctg ctgcgcgggc ttacaggttc cgctcgccgc ctgcctgttc
      541 ctcgggcaaa gatacacagt ctgggcgatc ctcaccggcg tggaggagca tctggtggag
      601 ctagtatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
      661 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
      721 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
      781 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
      841 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
      901 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
      961 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     1021 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     1081 acggcgccct gaagggtgag gaaaaggcga ggctgaagct gaaggacggc ggccactacg
     1141 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     1201 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     1261 acgaacgcgc cgagggtcgt cactctaccg gcggcatgga cgagctgtac aagtaaacac
     1321 gacgaattca ttgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     1381 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     1441 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     1501 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     1561 tcatgtctgg atccgtaccg agctcgcgta atcatggtca tagctgtttc ctgtgtgaaa
     1621 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1681 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     1741 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1801 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1861 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1921 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1981 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2041 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2101 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2161 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     2221 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2341 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2401 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2461 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2521 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2581 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2641 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2701 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2761 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2821 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2881 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2941 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3001 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3061 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3121 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3181 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3241 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3301 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3361 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3421 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3481 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3541 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3601 gaaatgttga atactcatac tcttcctttt tcaataagcg gccgcggcca tgccggccac
     3661 tagtctcgag ttattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3721 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     3781 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     3841 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     3901 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     3961 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     4021 ttgtactgag agtgcaccat atgaagacgt cgcctcctca ctacttctgg aatagctcag
     4081 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcgc gccattcgcc
     4141 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     4201 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     4261 gtcacgacgt tgtaaaacga cggccagtgc catctgcagt gaataataaa atgtgtgttt
     4321 gtccgaaata cgcgtttgag atttctgtcc cgactaaatt catgtcgcgc gatagtggtg
     4381 tttatcgccg atagagatgg cgatattgga aaaatcgata tttgaaaata tggcatattg
     4441 aaaatgtcgc cgatgtgagt ttctgtgtaa ctgatatcgc catttttcca aaagttgatt
     4501 tttgggcata cgcgatatct ggcgatacgc ttatatcgtt tacgggggat ggcgatagac
     4561 gcctttggtg acttgggcga ttctgtgtgt cgcaaatatc gcagtttcga tataggtgac
     4621 agacgatatg aggctatatc gccgatagag gcgacatcaa gctggcacat ggccaatgca
     4681 tatcgatcta tacattgaat caatattggc cattagccat attattcatt ggttatatag
     4741 cataaatcaa tattggctat tggccattgc atacgttgta tccatatcat aatatgtaca
     4801 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
     4861 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     4921 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     4981 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     5041 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     5101 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     5161 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     5221 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     5281 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     5341 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     5401 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
     5461 gctgttttga cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt
     5521 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
     5581 ggcccacccc cttggcttct tatgcatgct atactgtttt tggcttgggg tctatacacc
     5641 cccgcttcct catgttatag gtgatggtat agcttagcct ataggtgtgg gttattgacc
     5701 attattgacc actcccctat tggtgacgat actttccatt actaatccat aacatggctc
     5761 tttgccacaa ctctctttat tggctatatg ccaatacact gtccttcaga gactgacacg
     5821 gactctgtat ttttacagga tggggtctca tttattattt acaaattcac atatacaaca
     5881 ccaccgtccc cagtgcccgc agtttttatt aaacataacg tgggatctcc acgcgaatct
     5941 cgggtacgtg ttccggacat gggctcttct ccggtagcgg cggagcttct acatccgagc
     6001 cctgctccca tgcctccagc gactcatggt cgctcggcag ctccttgctc ctaacagtgg
     6061 aggccagact taggcacagc acgatgccca ccaccaccag tgtgccgcac aaggccgtgg
     6121 cggtagggta tgtgtctgaa aatgagctcg gggagcgggc ttgcaccgct gacgcatttg
     6181 gaagacttaa ggcagcggca gaagaagatg caggcagctg agttgttgtg ttctgataag
     6241 agtcagaggt aactcccgtt gcggtgctgt taacggtgga gggcagtgta gtctgagcag
     6301 tactcgttgc tgccgcgcgc gccaccagac ataatagctg acagactaac agactgttcc
     6361 tttccatggg tcttttctgc agtcaccgtc cttgacacg
//
