LOCUS       Exported                9363 bp ds-DNA     circular SYN 21-AUG-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Addgene207874_p0742_GW1_mScarlet_hEpac2_GeNL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   TANTAMA LAB
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 21, 2023 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9363
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     272..288
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        837..1216
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1217..1420
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2583..2591
                     /regulatory_class="other"
                     /note="Kozak sequence"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2588..6679
                     /codon_start=1
                     /note="mScarlet_hEpac2_GeNL"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYKAGTPVGTHEMEEELAEAVALLSQRGPDALLTVALRKPPGQRTDEELDLI
                     FEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVT
                     HGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFNRIIKDVEAKTM
                     RLEEHGKVVLVLERASQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEAMGPDSSAHDQ
                     TETFLSDFLLTHRVFMPSAQLCAALLHHFHVEPAGGSEQERSTYVCNKRQQILRLVSQW
                     VALYGSMLHTDPVATSFLQKLSDLVGRDTRLSNLLREQWPERRRCHRLENGCGNASPQM
                     KARNLPVWLPNQDEPLPGSSCAIQVGDKVPYDICRPDHSVLTLQLPVTASVREVMAALA
                     QEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEAHELIPHPDQLG
                     PTVGSAEGLDLVSAKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFM
                     RRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAIS
                     HLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALAKLSPPVIPFMPLLLKDMTFIH
                     EGNHTLVENLINFEKMRMMARVARMLHHCRSHNPVPLSPLRSRVSHLHEDSQVARISTC
                     SEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEPEQVSKGEEDNMASLPATHELH
                     IFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPD
                     GMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPV
                     MTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLK
                     NQPMYVFRKTELKHSKTELNFKEWQKAFTGFEDFVGDWRQTAGYNLDQVLEQGGVSSLF
                     QNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKV
                     ILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSL
                     LFRVTINGVTGWRLCERILA"
     CDS             2588..3283
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /note="mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     CDS             3265..3283
                     /codon_start=1
                     /product="monomeric super enhanced variant of CFP (Matsuda 
                     et al., 2008)"
                     /note="mseCFP"
                     /note="mammalian codon-optimized"
                     /translation="MDELYK"
     CDS             3265..3283
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /note="mECFP"
                     /note="mammalian codon-optimized"
                     /translation="MDELYK"
     CDS             3265..3283
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /note="CFP"
                     /note="mammalian codon-optimized"
                     /translation="MDELYK"
     CDS             3293..5491
                     /codon_start=1
                     /note="hEpac1(149-881)"
                     /translation="PVGTHEMEEELAEAVALLSQRGPDALLTVALRKPPGQRTDEELDL
                     IFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVV
                     THGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFNRIIKDVEAKT
                     MRLEEHGKVVLVLERASQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEAMGPDSSAHD
                     QTETFLSDFLLTHRVFMPSAQLCAALLHHFHVEPAGGSEQERSTYVCNKRQQILRLVSQ
                     WVALYGSMLHTDPVATSFLQKLSDLVGRDTRLSNLLREQWPERRRCHRLENGCGNASPQ
                     MKARNLPVWLPNQDEPLPGSSCAIQVGDKVPYDICRPDHSVLTLQLPVTASVREVMAAL
                     AQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEAHELIPHPDQL
                     GPTVGSAEGLDLVSAKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERF
                     MRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAI
                     SHLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALAKLSPPVIPFMPLLLKDMTFI
                     HEGNHTLVENLINFEKMRMMARVARMLHHCRSHNPVPLSPLRSRVSHLHEDSQVARIST
                     CSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP"
     CDS             5498..6172
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /note="mNeonGreen"
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFT"
     CDS             6179..6679
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /note="Nluc"
                     /note="human codon-optimized"
                     /translation="EDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSG
                     ENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMI
                     DYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCER
                     ILA"
     polyA_signal    6802..6936
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6960..6976)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6984..7000
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7008..7038)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7053..7074
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7362..7950)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8121..8981)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgaagacgt cgcctcctca
       61 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
      121 tagtcagcgc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
      181 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
      241 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc catctgcagt
      301 gaataataaa atgtgtgttt gtccgaaata cgcgtttgag atttctgtcc cgactaaatt
      361 catgtcgcgc gatagtggtg tttatcgccg atagagatgg cgatattgga aaaatcgata
      421 tttgaaaata tggcatattg aaaatgtcgc cgatgtgagt ttctgtgtaa ctgatatcgc
      481 catttttcca aaagttgatt tttgggcata cgcgatatct ggcgatacgc ttatatcgtt
      541 tacgggggat ggcgatagac gcctttggtg acttgggcga ttctgtgtgt cgcaaatatc
      601 gcagtttcga tataggtgac agacgatatg aggctatatc gccgatagag gcgacatcaa
      661 gctggcacat ggccaatgca tatcgatcta tacattgaat caatattggc cattagccat
      721 attattcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta
      781 tccatatcat aatatgtaca tttatattgg ctcatgtcca acattaccgc catgttgaca
      841 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      901 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      961 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     1021 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     1081 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     1141 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     1201 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     1261 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     1321 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     1381 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
     1441 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     1501 cctccgcggc cgggaacggt gcattggaac gcggattccc cgtgccaaga gtgacgtaag
     1561 taccgcctat agagtctata ggcccacccc cttggcttct tatgcatgct atactgtttt
     1621 tggcttgggg tctatacacc cccgcttcct catgttatag gtgatggtat agcttagcct
     1681 ataggtgtgg gttattgacc attattgacc actcccctat tggtgacgat actttccatt
     1741 actaatccat aacatggctc tttgccacaa ctctctttat tggctatatg ccaatacact
     1801 gtccttcaga gactgacacg gactctgtat ttttacagga tggggtctca tttattattt
     1861 acaaattcac atatacaaca ccaccgtccc cagtgcccgc agtttttatt aaacataacg
     1921 tgggatctcc acgcgaatct cgggtacgtg ttccggacat gggctcttct ccggtagcgg
     1981 cggagcttct acatccgagc cctgctccca tgcctccagc gactcatggt cgctcggcag
     2041 ctccttgctc ctaacagtgg aggccagact taggcacagc acgatgccca ccaccaccag
     2101 tgtgccgcac aaggccgtgg cggtagggta tgtgtctgaa aatgagctcg gggagcgggc
     2161 ttgcaccgct gacgcatttg gaagacttaa ggcagcggca gaagaagatg caggcagctg
     2221 agttgttgtg ttctgataag agtcagaggt aactcccgtt gcggtgctgt taacggtgga
     2281 gggcagtgta gtctgagcag tactcgttgc tgccgcgcgc gccaccagac ataatagctg
     2341 acagactaac agactgttcc tttccatggg tcttttctgc agtcaccgtc cttgacacga
     2401 agcttcccgg ccacagcaat cgatccatga cacaaccagt gacaagcagt cccccagtga
     2461 aaccgcatcc gcatccgagt ccgataccga taaagattct gaatcggagt gagtgccgcg
     2521 tccgagagcg ttccctgtcc acgtccacca tcggcggagc aggtgtgcct gaggcctcta
     2581 gaccaccatg gtgagcaagg gcgaggcagt gatcaaggag ttcatgcggt tcaaggtgca
     2641 catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     2701 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttctc
     2761 ctgggacatc ctgtcccctc agttcatgta cggctccagg gccttcacca agcaccccgc
     2821 cgacatcccc gactactata agcagtcctt ccccgagggc ttcaagtggg agcgcgtgat
     2881 gaacttcgag gacggcggcg ccgtgaccgt gacccaggac acctccctgg aggacggcac
     2941 cctgatctac aaggtgaagc tccgcggcac caacttccct cctgacggcc ccgtaatgca
     3001 gaagaagaca atgggctggg aagcgtccac cgagcggttg taccccgagg acggcgtgct
     3061 gaagggcgac attaagatgg ccctgcgcct gaaggacggc ggccgctacc tggcggactt
     3121 caagaccacc tacaaggcca agaagcccgt gcagatgccc ggcgcctaca acgtcgaccg
     3181 caagttggac atcacctccc acaacgagga ctacaccgtg gtggaacagt acgaacgctc
     3241 cgagggccgc cactccaccg gcggcatgga cgagctgtac aaggctggta cccccgtggg
     3301 aactcatgag atggaggagg agttggccga agctgtggcc ctgctctccc agcgggggcc
     3361 tgacgccctg ctcactgtgg cacttcgaaa gcccccaggt cagcgcacgg atgaagagct
     3421 ggacctcatc tttgaggagc tgctgcacat caaggctgtg gcccacctct ccaactcggt
     3481 gaagcgagaa ttagcggctg ttctgctctt tgaaccacac agcaaggcag ggaccgtgtt
     3541 gttcagccag ggggacaagg gcacttcgtg gtacattatc tggaagggat ctgtcaacgt
     3601 ggtgacccat ggcaaggggc tggtgaccac cctgcatgag ggagatgatt ttggacagct
     3661 ggctctggtg aatgatgcac cccgggcagc caccatcatc ctgcgagaag acaactgtca
     3721 tttcctgcgt gtggacaagc aggacttcaa ccgtatcatc aaggatgtgg aggcaaagac
     3781 catgcggctg gaagaacatg gcaaagtggt gctggtgctg gagagagcct ctcagggcgc
     3841 cggcccttcc cgacccccaa ccccaggcag gaaccggtat acagtgatgt ctggcacccc
     3901 agagaagatc ctagagcttc tgttggaggc catgggacca gattccagtg ctcatgacca
     3961 aacagagaca ttcctcagcg acttcctcct gacccacagg gtcttcatgc ccagcgccca
     4021 actctgcgct gcccttctgc accacttcca tgtggagcct gcgggtggca gcgagcagga
     4081 gcgcagcacc tacgtctgca acaagaggca gcagatcttg cggctggtca gccagtgggt
     4141 ggccctgtat ggctccatgc tccacactga ccctgtggcc accagcttcc tccagaaact
     4201 ctcagacctg gtgggcaggg acacccgact cagcaacctg ctgagggagc agtggccaga
     4261 gaggcggcga tgccacaggt tggagaatgg ctgtgggaat gcatctcctc agatgaaggc
     4321 ccggaacttg cctgtttggc tccccaacca ggacgagccc cttcctggca gcagctgtgc
     4381 catccaagtt ggggataaag tcccctatga catctgccgg ccagaccact cagtgttgac
     4441 cctgcagctg cctgtgacag cctccgtgag agaggtgatg gcagcgttgg cccaggagga
     4501 tggctggacc aaggggcagg tgctggtgaa ggtcaattct gcaggtgatg ccattggcct
     4561 gcagccagat gcccgtggtg tggccacatc tctggggctc aatgagcgtc tctttgttgt
     4621 caacccacag gaagcgcatg agctgatccc acaccctgac cagctggggc ccactgtggg
     4681 ctctgctgag gggctggacc tggtgagtgc caaggacctg gcaggccagc tgacggacca
     4741 cgactggagc ctcttcaaca gtatccacca ggtggagctg atccactatg tgctgggccc
     4801 ccagcatctg cgggatgtca ccaccgccaa cctggagcgc ttcatgcgcc gcttcaatga
     4861 gctgcagtac tgggtggcca ccgagctgtg tctctgcccc gtgcccggcc cccgggccca
     4921 gctgctcagg aagttcatta agctggcggc ccacctcaag gagcagaaga atctcaattc
     4981 cttctttgcc gtcatgtttg gcctcagcaa ctcggccatc agccacctag cccacacctg
     5041 ggagcggctg ccccacaaag tccggaagct gtactccgcc ctcgagaggc tgctggaccc
     5101 ctcatggaac caccgggtat accgactggc cctcgccaag ctctcccctc ctgtcatccc
     5161 cttcatgccc cttcttctca aagacatgac cttcattcat gagggaaacc acacactagt
     5221 ggagaatctc atcaactttg agaagatgag aatgatggcc agagtcgcgc ggatgctgca
     5281 ccactgccga agccacaacc ctgtgcctct ctcaccactc agaagccgag tttcccacct
     5341 ccacgaggac agccaggtgg cgaggatttc cacatgctcg gagcagtccc tgagcacccg
     5401 gagtccagcc agcacctggg cttatgtcca gcagctgaag gtcattgaca accagcggga
     5461 actctcccgc ctctcccgag agctggagcc agagcaagtc agcaagggcg aggaggataa
     5521 catggcctct ctcccagcga cacatgagtt acacatcttt ggctccatca acggtgtgga
     5581 ctttgacatg gtgggtcagg gcaccggcaa tccaaatgat ggttatgagg agttaaacct
     5641 gaagtccacc aagggtgacc tccagttctc cccctggatt ctggtccctc atatcgggta
     5701 tggcttccat cagtacctgc cctaccctga cgggatgtcg cctttccagg ccgccatggt
     5761 agatggctcc ggctaccaag tccatcgcac aatgcagttt gaagatggtg cctcccttac
     5821 tgttaactac cgctacacct acgagggaag ccacatcaaa ggagaggccc aggtgaaggg
     5881 gactggtttc cctgctgacg gtcctgtgat gaccaactcg ctgaccgctg cggactggtg
     5941 caggtcgaag aagacttacc ccaacgacaa aaccatcatc agtaccttta agtggagtta
     6001 caccactgga aatggcaagc gctaccggag cactgcgcgg accacctaca cctttgccaa
     6061 gccaatggcg gctaactatc tgaagaacca gccgatgtac gtgttccgta agacggagct
     6121 caagcactcc aagaccgagc tcaacttcaa ggagtggcaa aaggcgttca ccggcttcga
     6181 agatttcgtt ggggactggc gacagacagc cggctacaac ctggaccaag tccttgaaca
     6241 gggaggtgtg tccagtttgt ttcagaatct cggggtgtcc gtaactccga tccaaaggat
     6301 tgtcctgagc ggtgaaaatg ggctgaagat cgacatccat gtcatcatcc cgtatgaagg
     6361 tctgagcggc gaccaaatgg gccagatcga aaaaattttt aaggtggtgt accctgtgga
     6421 tgatcatcac tttaaggtga tcctgcacta tggcacactg gtaatcgacg gggttacgcc
     6481 gaacatgatc gactatttcg gacggccgta tgaaggcatc gccgtgttcg acggcaaaaa
     6541 gatcactgta acagggaccc tgtggaacgg caacaaaatt atcgacgagc gcctgatcaa
     6601 ccccgacggc tccctgctgt tccgagtaac catcaacgga gtgaccggct ggcggctgtg
     6661 cgaacgcatt ctggcctaaa ctcgacgaat tcattgatca taatcagcca taccacattt
     6721 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     6781 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     6841 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6901 tccaaactca tcaatgtatc ttatcatgtc tggatccgta ccgagctcgc gtaatcatgg
     6961 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7021 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7081 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7141 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7201 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7261 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7321 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7381 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7441 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7501 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     7561 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7621 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7681 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7741 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7801 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7861 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7921 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7981 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8041 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8101 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8161 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8221 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8281 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8341 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8401 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8461 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8521 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8581 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8641 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8701 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8761 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8821 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8881 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8941 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaataa
     9001 gcggccgcgg ccatgccggc cactagtctc gagttattat tgaagcattt atcagggtta
     9061 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9121 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     9181 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     9241 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     9301 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     9361 taa
//
