LOCUS       pDual                   7577 bp ds-DNA     circular     10-AUG-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    909..943
                     /label="BBa_J23108"
     misc_feature    991..997
                     /label="RBS"
     misc_feature    1005..3284
                     /label="I-OnuI TSM"
     misc_feature    1671..3032
                     /label="VMA1 L212P"
     misc_feature    3307..3580
                     /label="Aph 3' Terminator"
     misc_feature    3624..3658
                     /label="BBa_J23100"
     misc_feature    3689..3695
                     /label="RBS"
     misc_feature    3703..5997
                     /label="I-GpeMI TSM"
     misc_feature    4384..5745
                     /label="VMA1 L212P"
     misc_feature    6392..6572
                     /label="p3 promoter"
     misc_feature    6597..7248
                     /label="AmpR"
     misc_feature    7413..381
                     /label="p15A origin"
ORIGIN
        1 GGCGCATGAC TTCAAGACTA ACTCCTCTAA ATCAATTACC AGTGGCTGCT GCCAGTGGTG
       61 CTTTTGCATG TCTTTCCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT
      121 CGGACTGAAC GGGGGGTTCG TGCATACAGT CCAGCTTGGA GCGAACTGCC TACCCGGAAC
      181 TGAGTGTCAG GCGTGGAATG AGACAAACGC GGCCATAACA GCGGAATGAC ACCGGTAAAC
      241 CGAAAGGCAG GAACAGGAGA GCGCACGAGG GAGCCGCCAG GGGGAAACGC CTGGTATCTT
      301 TATAGTCCTG TCGGGTTTCG CCACCACTGA TTTGAGCGTC AGATTTCGTG ATGCTTGTCA
      361 GGGGGGCGGA GCCTATGGAA AAACGGCTTT GCCGCGGCCC TCTCACTTCC CTGTTAAGTA
      421 TCTTCCTGGC ATCTTCCAGG AAATCTCCGC CCCGTTCGTA AGCCATTTCC GCTCGCCGCA
      481 GTCGAACGAC CGAGCGTAGC GAGTCAGTGA GCGAGGAAGC GGAATATATC CTGTATCACA
      541 TATTCTGCTG ACGCACCGGT GCAGCCTTTT TTCTCCTGCC ACATGAAGCA CTTCACTGAC
      601 ACCCTCATCA GTGCCAACAT AGTAAGCCAG TATACACTCC GCTAGCGCTG AGGTCTGCCT
      661 CGTGAAGAAG GTGTTGCTGA CTCATACCAG GCCTGAATCG CCCCATCATC CAGCCAGAAA
      721 GTGAGGGAGC CACGGTTGAT GAGAGCTTTG TTGTAGGTGG ACCAGTTGGT GATTTTGAAC
      781 TTTTGCTTTG CCACGGAACG GTCTGCGTTG TCGGCATGCG CATAATGTGC CTGTCAAATG
      841 GACGAAGCAG GGATTCTGCA AACCCTATGC TACTCCGTCA AGCCGTCAAT TGTCTGATTC
      901 GTTACCAAct gacagctagc tcagtcctag gtataatgct agcTCCATAC CCGTTTTTTT
      961 GGGCTAGAAA TAATTTTGTT TAACTTTAAG AAGGAGATAT ACCCATGGGA TCCGCCTACA
     1021 TGTCCCGTCG CGAGTCCATT AACCCGTGGA TTCTCACCGG TTTCGCCGAC GCGGAAGGCT
     1081 CCTTTTTGCT GCGCATCCGC AACAACAACA AGTCCAGCGT CGGCTACTCC ACTGAGCTCG
     1141 GCTTCCAAAT TACACTTCAT AACAAGGACA AGAGCATTCT TGAGAACATC CAGTCAACAT
     1201 GGAAGGTGGG CGTGATCGCC AACAGCGGTG ACAACGCCGT GTCGCTGAAG GTCACGCGTT
     1261 TTGAGGACCT GAAGGTCATT ATCGACCATT TTGAAAAATA CCCACTGATT ACGCAGAAGC
     1321 TCGGTGACTA CATGCTGTTT AAGCAGGCGT TTTGCGTCAT GGAGAACAAG GAGCATTTGA
     1381 AGATTAATGG TATCAAGGAG CTGGTGCGCA TTAAGGCAAA GCTCAATTGG GGTCTGACGG
     1441 ATGAGCTGAA GAAGGCCTTT CCGGAGATCA TCTCGAAGGA GCGCTCCCTC ATCAACAAGA
     1501 ACATCCCTAA TTTCAAGTGG CTGGCGGGTT TTACCTCGGG CGAGGGTTGC TTCTTTGTTA
     1561 ACCTGATCAA GTCAAAGTCG AAGCTAGGTG TCCAGGTGCA GCTGGTGTTC AGCATTACCC
     1621 AACACATCAA GGATAAGAAC CTCATGAACT CTCTGATTAC CTACTTGGGC TGCTTTGCCA
     1681 AGGGTACCAA TGTTTTAATG GCGGATGGGT CTATTGAATG TATTGAAAAC ATTGAGGTTG
     1741 GTAATAAGGT CATGGGTAAA GATGGCAGAC CTCGTGAGGT AATTAAATTG CCCAGAGGAA
     1801 GAGAAACTAT GTACAGCGTC GTGCAGAAAA GTCAGCACAG AGCCCACAAA AGTGACTCAA
     1861 GTCGTGAAGT GCCAGAATTA CTCAAGTTTA CGTGTAATGC GACCCATGAG TTGGTTGTTA
     1921 GAACACCTCG TAGTGTCCGC CGTTTGTCTC GTACCATTAA GGGTGTCGAA TATTTTGAAG
     1981 TTATTACTTT TGAGATGGGC CAAAAGAAAG CCCCCGACGG TAGAATTGTT GAGCTTGTCA
     2041 AGGAAGTTTC AAAGAGCTAC CCAATATCTG AGGGGCCTGA GAGAGCCAAC GAATTAGTAG
     2101 AATCCTATAG AAAGGCTTCA AATAAAGCTT ATTTTGAGTG GACTATTGAG GCCAGAGATC
     2161 TTTCTCTGTT GGGTTCCCAT GTTCGTAAAG CTACCTACCA GACTTACGCT CCAATTCTTT
     2221 ATGAGAATGA CCACTTTTTC GACTACATGC AAAAAAGTAA GTTTCATCTC ACCATTGAAG
     2281 GTCCAAAAGT ACTTGCTTAT TTACCTGGTT TATGGATTGG TGATGGATTG TCTGACAGGG
     2341 CAACTTTTTC GGTTGATTCC AGAGATACTT CTTTGATGGA ACGTGTTACT GAATATGCTG
     2401 AAAAGTTGAA TTTGTGCGCC GAGTATAAGG ACAGAAAAGA ACCACAAGTT GCCAAAACTG
     2461 TTAATTTGTA CTCTAAAGTT GTCAGAGGTA ATGGTATTCG CAATAATCTT AATACTGAGA
     2521 ATCCATTATG GGACGCTATT GTTGGCTTAG GATTCTTGAA GGACGGTGTC AAAAATATTC
     2581 CTTCTTTCTT GTCTACGGAC AATATCGGTA CTCGTGAAAC ATTTCTTGCT GGTCTAATTG
     2641 ATTCTGATGG CTATGTTACT GATGAGCATG GTATTAAAGC AACAATAAAG ACAATTCATA
     2701 CTTCTGTCAG AGATGGTTTG GTTTCCCTTG CTCGTTCTTT AGGCTTAGTA GTCTCGGTTA
     2761 ACGCAGAACC TGCTAAGGTT GACATGAATG GCACCAAACA TAAAATTAGT TATGCTATTT
     2821 ATATGTCTGG TGGAGATGTT TTGCTTAACG TTCTTTCGAA GTGTGCCGGC TCTAAAAAAT
     2881 TCAGGCCTGC TCCCGCCGCT GCTTTTGCAC GTGAGTGCCG CGGATTTTAT TTCGAGTTAC
     2941 AAGAATTGAA GGAAGACGAT TATTATGGGA TTACTTTATC TGATGATTCT GATCATCAGT
     3001 TTTTGCTTGC CAACCAGGTT GTCGTCCATA ATTGCGGCTA CATTAAGGAG AAAAACAAGA
     3061 GTGAGTTCTC CTGGCTTGAC TTCGTCGTCA CGAAATTCTC CGACATCAAC GACAAGATCA
     3121 TTCCGGTCTT TCAGGAAAAC ACGCTCATCG GCGTGAAGCT CGAGGACTTC GAGGATTGGT
     3181 GTAAGGTCGC TAAGCTGATC GAGGAGAAAA AGCACCTGAC AGAAAGTGGC CTGGACGAGA
     3241 TCAAGAAGAT TAAGCTGAAC ATGAACAAGG GCAGAGTATT CTAAGCGGCC GCTCAGAATT
     3301 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGGCTTTGT
     3361 TGAATAAATC GAACTTTTGC TGAGTTGAAG GATCAGATCA CGCATCTTCC CGACAACGCA
     3421 GACCGTTCCG TGGCAAAGCA AAAGTTCAAA ATCACCAACT GGTCCACCTA CAACAAAGCT
     3481 CTCATCAACC GTGGCTCCCT CACTTTCTGG CTGGATGATG GGGCGATTCA GGCCTGGTAT
     3541 GAGTCAGCAA CACCTTCTTC ACGAGGCAGA CCTCAGCGCT CAAAGATGCA GGGGTAAAAG
     3601 CTAACCGCAT CTTTACCGAC AAGttgacgg ctagctcagt cctaggtaca gtgctagcGC
     3661 ATCCGGCAGT TCAACAGATC GGGAAGGGAA GGAGATATAC CCATGGGCCC TACCAGAAAC
     3721 GAGAGTATAA ATCCTTGGGT GCTGACTGGA TTCGCCGATG CAGAAGGGAG TTTCATCCTG
     3781 CGTATTCGGA ATAACAATAA AAGTTCCGCC GGATACAGTA CGGAACTTGG GTTTCAAATA
     3841 ACGCTGCACA AAAAAGACAT ATCTATTTTG GAGAATATAC AATCCACGTG GAAGGTTGGT
     3901 GTGATCGCCA ATAGCGGTGA CAATGCTGTC AGCTTAAAAG TCACCAGATT CGAGGACTTA
     3961 CGCGTTGTGC TTAATCATTT CGAGAAATAC CCCCTGATTA CTCAGAAACT TGGTGACTAT
     4021 CTTTTGTTTA AGCAAGCGTT CAGCGTGATG GAAAATAAGG AGCACCTGAA AATAGAAGGA
     4081 ATCAAGAGAC TGGTCGGGAT TAAGGCCAAC CTGAATTGGG GACTTACTGA CGAGTTGAAG
     4141 GAAGCGTTTG TTGCCTCCGG CGGCGAGAAT ATTTTCGTTG CAAGTGGGGG GGAACGTAGC
     4201 CTTATTAACA AGAACATACC TAATAGCGGT TGGTTAGCAG GATTTACGAG TGGGGAGGGG
     4261 TGTTTCTTCG TTAGTTTAAT TAAAAGCAAG TCCAAGCTTG GAGTACAGGT ACAACTTGTG
     4321 TTTTCAATTA CTCAACACGC TCGGGACAGA GCCCTGATGG ATAACTTGGT TACGTATTTA
     4381 GGATGCTTTG CaAAGGGTAC aAATGTTTTg ATGGCGGAcG GGTCTATcGA ATGTATcGAA
     4441 AACATcGAGG TTGGcAATAA GGTgATGGGT AAgGATGGCc GtCCTCGTGA aGTAATTAAg
     4501 TTGCCCcGtG GAAGAGAgAC TATGTAtAGC GTCGTtCAGA AAAGcCAGCA CcGtGCCCAC
     4561 AAgAGTGACa gcAGTCGTGA gGTGCCAGAg TTACTCAAaT TTACGTGcAA TGCGACaCAT
     4621 GAGcTGGTTG TTcGtACACC TCGcAGTGTC CGtCGTTTGa gcCGTACCAT cAAGGGTGTg
     4681 GAATATTTcG AAGTTATcAC TTTTGAaATG GGCCAgAAGA AAGCaCCCGA CGGcAGAATT
     4741 GTgGAGCTTG TgAAGGAAGT gTCAAAGAGt TACCCAATtT CTGAGGGtCC TGAGCGtGCC
     4801 AACGAgTTAG TAGAgTCCTA TcGtAAGGCT agcAATAAAG CcTATTTTGA aTGGACTATc
     4861 GAGGCCcGtG ATCTTagTCT GTTGGGcTCC CATGTgCGTA AAGCcACCTA CCAAACTTAC
     4921 GCcCCAATTC TgTATGAGAA cGACCACTTc TTCGACTAtA TGCAAAAgAG TAAGTTcCAT
     4981 CTCACaATTG AAGGcCCAAA AGTgCTTGCT TAcTTACCTG GcTTATGGAT cGGTGATGGc
     5041 TTGTCTGAtA GGGCAACcTT TTCGGTgGAT TCCcGtGATA CTagcTTGAT GGAgCGTGTT
     5101 ACcGAATATG CcGAAAAGct GAATTTGTGt GCCGAGTAcA AGGACcGtAA AGAACCgCAA
     5161 GTTGCaAAAA CTGTgAATTT GTAtTCTAAA GTgGTCAGAG GcAATGGTAT cCGCAATAAc
     5221 CTTAAcACcG AGAATCCgTT ATGGGAtGCT ATTGTgGGCT TAGGcTTCTT GAAaGACGGT
     5281 GTgAAAAATA TcCCTTCTTT tTTGTCTACc GACAATATtG GTACTCGcGA AACATTcCTT
     5341 GCTGGcCTAA TTGAcTCTGA TGGtTATGTT ACcGATGAGC AcGGTATcAA gGCAACAATt
     5401 AAGACAATcC ATACTagcGT CcGtGAcGGT TTGGTgTCCC TTGCcCGTTC TcTgGGCTTA
     5461 GTgGTCTCGG TgAACGCAGA gCCTGCTAAa GTTGACATGA AcGGCACaAA ACATAAgATT
     5521 AGTTAcGCTA TTTAcATGTC TGGcGGAGAT GTgTTGCTTA AtGTTCTTTC aAAGTGTGCa
     5581 GGCTCTAAgA AATTCcGtCC TGCTCCgGCC GCTGCcTTTG CACGcGAGTG CCGtGGATTT
     5641 TAcTTCGAGc TgCAAGAATT aAAGGAAGAt GATTATTAcG GGATTACcTT ATCTGAcGAT
     5701 TCTGAcCATC AGTTcTTGCT TGCaAACCAG GTgGTCGTCC AcAATTGCGG GTATATCAAG
     5761 GAGAAAAAGA AAAGCGAGTT TTCTTGGCTG GAATTTGTGG TCACAAAGTT CTCTGACATA
     5821 AAAGACAAGA TTATACCGGT CTTTCAAGTG AATAATATTA TAGGTGTCAA ACTTGAGGAC
     5881 TTCGAAGACT GGTGCAAGGT GGCCAAGCTG ATTGAGGAGA AGAAGCATTT AACTGAAAGC
     5941 GGTTTGGAAG AAATCCGCAA TATCAAATTG AACATGAACA AAGGGAGAGT ATTATAACTG
     6001 GATTTGCTGA GGATGAAGGT GGAGGAAGGT GATGTCATTC TGGTGAAGAA GCTCGACCGT
     6061 CTTGGCCGCG ACACCGCCGA CATGATCCAA CTGATAAAAG AGTTTGATGC TCAGGGTGTA
     6121 GCGGTTCGGT TTATTGACGA CGGGATCAGT ACCGACGGTG ATATGGGGCA AATGGTGGTC
     6181 ACCATCCTGT CGGCTGTGGC ACAGGCTGAA CGCCGGAGGA TCCTAGAGCG CACGAATGAG
     6241 GGCCGACAGG AAGCAAAGCT GAAAGGAATC AAATTTGGCC GCAGGCGTAC CGTGGACAGG
     6301 AACGTCGTGC TGACGCTTCA TCAGAAGGGC ACTGGTGCAA CGGAAATTGC TCATCAGCTC
     6361 AGTATTGCCC GCTCCACGGT TTATAAAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC
     6421 TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC
     6481 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC
     6541 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA
     6601 GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT
     6661 TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG
     6721 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG
     6781 AACGTTTTCC AATGATGAGC ACTTTTAAAG GGACCGAAGG AGCTAACCGC TTTTTTGCAC
     6841 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA
     6901 CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA
     6961 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG
     7021 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT
     7081 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT
     7141 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA
     7201 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA
     7261 GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG
     7321 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC
     7381 TGAGCGTCAG ACCCCTTAAT AAGATGATCT TCTTGAGATC GTTTTGGTCT GCGCGTAATC
     7441 TCTTGCTCTG AAAACGAAAA AACCGCCTTG CAGGGCGGTT TTTCGAAGGT TCTCTGAGCT
     7501 ACCAACTCTT TGAACCGAGG TAACTGGCTT GGAGGAGCGC AGTCACCAAA ACTTGTCCTT
     7561 TCAGTTTAGC CTTAACC
//