LOCUS       pEndo-I-OnuITSM         5233 bp ds-DNA     circular     10-AUG-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    909..943
                     /label="BBa_J23108"
     misc_feature    991..997
                     /label="RBS"
     misc_feature    1005..3284
                     /label="I-OnuI TSM"
     CDS             1005..3284
                     /translation="MGSAYMSRRESINPWILTGFADAEGSFLLRIRNNNKSSVGYSTELGFQITLHNKDKSILENIQSTWKVGVIANSGDNAVSLKVTRFEDLKVIIDHFEKYPLITQKLGDYMLFKQAFCVMENKEHLKINGIKELVRIKAKLNWGLTDELKKAFPEIISKERSLINKNIPNFKWLAGFTSGEGCFFVNLIKSKSKLGVQVQLVFSITQHIKDKNLMNSLITYLGCFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRETMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEVITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARDLSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLPGLWIGDGLSDRATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRGNGIRNNLNTENPLWDAIVGLGFLKDGVKNIPSFLSTDNIGTRETFLAGLIDSDGYVTDEHGIKATIKTIHTSVRDGLVSLARSLGLVVSVNAEPAKVDMNGTKHKISYAIYMSGGDVLLNVLSKCAGSKKFRPAPAAAFARECRGFYFELQELKEDDYYGITLSDDSDHQFLLANQVVVHNCGYIKEKNKSEFSWLDFVVTKFSDINDKIIPVFQENTLIGVKLEDFEDWCKVAKLIEEKKHLTESGLDEIKKIKLNMNKGRVF*"
     misc_feature    1671..3032
                     /label="VMA1 L212P"
     CDS             1671..3032
                     /translation="CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRETMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEVITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARDLSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLPGLWIGDGLSDRATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRGNGIRNNLNTENPLWDAIVGLGFLKDGVKNIPSFLSTDNIGTRETFLAGLIDSDGYVTDEHGIKATIKTIHTSVRDGLVSLARSLGLVVSVNAEPAKVDMNGTKHKISYAIYMSGGDVLLNVLSKCAGSKKFRPAPAAAFARECRGFYFELQELKEDDYYGITLSDDSDHQFLLANQVVVHN"
     misc_feature    3307..3580
                     /label="Aph 3' Term"
     misc_feature    4048..4228
                     /label="p3 promoter"
     misc_feature    4253..4904
                     /label="AmpR"
     misc_feature    5069..381
                     /label="p15A ori"
ORIGIN
        1 GGCGCATGAC TTCAAGACTA ACTCCTCTAA ATCAATTACC AGTGGCTGCT GCCAGTGGTG
       61 CTTTTGCATG TCTTTCCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT
      121 CGGACTGAAC GGGGGGTTCG TGCATACAGT CCAGCTTGGA GCGAACTGCC TACCCGGAAC
      181 TGAGTGTCAG GCGTGGAATG AGACAAACGC GGCCATAACA GCGGAATGAC ACCGGTAAAC
      241 CGAAAGGCAG GAACAGGAGA GCGCACGAGG GAGCCGCCAG GGGGAAACGC CTGGTATCTT
      301 TATAGTCCTG TCGGGTTTCG CCACCACTGA TTTGAGCGTC AGATTTCGTG ATGCTTGTCA
      361 GGGGGGCGGA GCCTATGGAA AAACGGCTTT GCCGCGGCCC TCTCACTTCC CTGTTAAGTA
      421 TCTTCCTGGC ATCTTCCAGG AAATCTCCGC CCCGTTCGTA AGCCATTTCC GCTCGCCGCA
      481 GTCGAACGAC CGAGCGTAGC GAGTCAGTGA GCGAGGAAGC GGAATATATC CTGTATCACA
      541 TATTCTGCTG ACGCACCGGT GCAGCCTTTT TTCTCCTGCC ACATGAAGCA CTTCACTGAC
      601 ACCCTCATCA GTGCCAACAT AGTAAGCCAG TATACACTCC GCTAGCGCTG AGGTCTGCCT
      661 CGTGAAGAAG GTGTTGCTGA CTCATACCAG GCCTGAATCG CCCCATCATC CAGCCAGAAA
      721 GTGAGGGAGC CACGGTTGAT GAGAGCTTTG TTGTAGGTGG ACCAGTTGGT GATTTTGAAC
      781 TTTTGCTTTG CCACGGAACG GTCTGCGTTG TCGGCATGCG CATAATGTGC CTGTCAAATG
      841 GACGAAGCAG GGATTCTGCA AACCCTATGC TACTCCGTCA AGCCGTCAAT TGTCTGATTC
      901 GTTACCAAct gacagctagc tcagtcctag gtataatgct agcTCCATAC CCGTTTTTTT
      961 GGGCTAGAAA TAATTTTGTT TAACTTTAAG AAGGAGATAT ACCCATGGGA TCCGCCTACA
     1021 TGTCCCGTCG CGAGTCCATT AACCCGTGGA TTCTCACCGG TTTCGCCGAC GCGGAAGGCT
     1081 CCTTTTTGCT GCGCATCCGC AACAACAACA AGTCCAGCGT CGGCTACTCC ACTGAGCTCG
     1141 GCTTCCAAAT TACACTTCAT AACAAGGACA AGAGCATTCT TGAGAACATC CAGTCAACAT
     1201 GGAAGGTGGG CGTGATCGCC AACAGCGGTG ACAACGCCGT GTCGCTGAAG GTCACGCGTT
     1261 TTGAGGACCT GAAGGTCATT ATCGACCATT TTGAAAAATA CCCACTGATT ACGCAGAAGC
     1321 TCGGTGACTA CATGCTGTTT AAGCAGGCGT TTTGCGTCAT GGAGAACAAG GAGCATTTGA
     1381 AGATTAATGG TATCAAGGAG CTGGTGCGCA TTAAGGCAAA GCTCAATTGG GGTCTGACGG
     1441 ATGAGCTGAA GAAGGCCTTT CCGGAGATCA TCTCGAAGGA GCGCTCCCTC ATCAACAAGA
     1501 ACATCCCTAA TTTCAAGTGG CTGGCGGGTT TTACCTCGGG CGAGGGTTGC TTCTTTGTTA
     1561 ACCTGATCAA GTCAAAGTCG AAGCTAGGTG TCCAGGTGCA GCTGGTGTTC AGCATTACCC
     1621 AACACATCAA GGATAAGAAC CTCATGAACT CTCTGATTAC CTACTTGGGC TGCTTTGCCA
     1681 AGGGTACCAA TGTTTTAATG GCGGATGGGT CTATTGAATG TATTGAAAAC ATTGAGGTTG
     1741 GTAATAAGGT CATGGGTAAA GATGGCAGAC CTCGTGAGGT AATTAAATTG CCCAGAGGAA
     1801 GAGAAACTAT GTACAGCGTC GTGCAGAAAA GTCAGCACAG AGCCCACAAA AGTGACTCAA
     1861 GTCGTGAAGT GCCAGAATTA CTCAAGTTTA CGTGTAATGC GACCCATGAG TTGGTTGTTA
     1921 GAACACCTCG TAGTGTCCGC CGTTTGTCTC GTACCATTAA GGGTGTCGAA TATTTTGAAG
     1981 TTATTACTTT TGAGATGGGC CAAAAGAAAG CCCCCGACGG TAGAATTGTT GAGCTTGTCA
     2041 AGGAAGTTTC AAAGAGCTAC CCAATATCTG AGGGGCCTGA GAGAGCCAAC GAATTAGTAG
     2101 AATCCTATAG AAAGGCTTCA AATAAAGCTT ATTTTGAGTG GACTATTGAG GCCAGAGATC
     2161 TTTCTCTGTT GGGTTCCCAT GTTCGTAAAG CTACCTACCA GACTTACGCT CCAATTCTTT
     2221 ATGAGAATGA CCACTTTTTC GACTACATGC AAAAAAGTAA GTTTCATCTC ACCATTGAAG
     2281 GTCCAAAAGT ACTTGCTTAT TTACCTGGTT TATGGATTGG TGATGGATTG TCTGACAGGG
     2341 CAACTTTTTC GGTTGATTCC AGAGATACTT CTTTGATGGA ACGTGTTACT GAATATGCTG
     2401 AAAAGTTGAA TTTGTGCGCC GAGTATAAGG ACAGAAAAGA ACCACAAGTT GCCAAAACTG
     2461 TTAATTTGTA CTCTAAAGTT GTCAGAGGTA ATGGTATTCG CAATAATCTT AATACTGAGA
     2521 ATCCATTATG GGACGCTATT GTTGGCTTAG GATTCTTGAA GGACGGTGTC AAAAATATTC
     2581 CTTCTTTCTT GTCTACGGAC AATATCGGTA CTCGTGAAAC ATTTCTTGCT GGTCTAATTG
     2641 ATTCTGATGG CTATGTTACT GATGAGCATG GTATTAAAGC AACAATAAAG ACAATTCATA
     2701 CTTCTGTCAG AGATGGTTTG GTTTCCCTTG CTCGTTCTTT AGGCTTAGTA GTCTCGGTTA
     2761 ACGCAGAACC TGCTAAGGTT GACATGAATG GCACCAAACA TAAAATTAGT TATGCTATTT
     2821 ATATGTCTGG TGGAGATGTT TTGCTTAACG TTCTTTCGAA GTGTGCCGGC TCTAAAAAAT
     2881 TCAGGCCTGC TCCCGCCGCT GCTTTTGCAC GTGAGTGCCG CGGATTTTAT TTCGAGTTAC
     2941 AAGAATTGAA GGAAGACGAT TATTATGGGA TTACTTTATC TGATGATTCT GATCATCAGT
     3001 TTTTGCTTGC CAACCAGGTT GTCGTCCATA ATTGCGGCTA CATTAAGGAG AAAAACAAGA
     3061 GTGAGTTCTC CTGGCTTGAC TTCGTCGTCA CGAAATTCTC CGACATCAAC GACAAGATCA
     3121 TTCCGGTCTT TCAGGAAAAC ACGCTCATCG GCGTGAAGCT CGAGGACTTC GAGGATTGGT
     3181 GTAAGGTCGC TAAGCTGATC GAGGAGAAAA AGCACCTGAC AGAAAGTGGC CTGGACGAGA
     3241 TCAAGAAGAT TAAGCTGAAC ATGAACAAGG GCAGAGTATT CTAAGCGGCC GCTCAGAATT
     3301 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGGCTTTGT
     3361 TGAATAAATC GAACTTTTGC TGAGTTGAAG GATCAGATCA CGCATCTTCC CGACAACGCA
     3421 GACCGTTCCG TGGCAAAGCA AAAGTTCAAA ATCACCAACT GGTCCACCTA CAACAAAGCT
     3481 CTCATCAACC GTGGCTCCCT CACTTTCTGG CTGGATGATG GGGCGATTCA GGCCTGGTAT
     3541 GAGTCAGCAA CACCTTCTTC ACGAGGCAGA CCTCAGCGCT CAAAGATGCA GGGGTAAAAG
     3601 CTAACCGCAT CTTTACCGAC AAGGCATCCG GCAGTTCAAC AGATCGGGAA GGGCTGGATT
     3661 TGCTGAGGAT GAAGGTGGAG GAAGGTGATG TCATTCTGGT GAAGAAGCTC GACCGTCTTG
     3721 GCCGCGACAC CGCCGACATG ATCCAACTGA TAAAAGAGTT TGATGCTCAG GGTGTAGCGG
     3781 TTCGGTTTAT TGACGACGGG ATCAGTACCG ACGGTGATAT GGGGCAAATG GTGGTCACCA
     3841 TCCTGTCGGC TGTGGCACAG GCTGAACGCC GGAGGATCCT AGAGCGCACG AATGAGGGCC
     3901 GACAGGAAGC AAAGCTGAAA GGAATCAAAT TTGGCCGCAG GCGTACCGTG GACAGGAACG
     3961 TCGTGCTGAC GCTTCATCAG AAGGGCACTG GTGCAACGGA AATTGCTCAT CAGCTCAGTA
     4021 TTGCCCGCTC CACGGTTTAT AAAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT
     4081 TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG
     4141 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
     4201 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT
     4261 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC
     4321 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG
     4381 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG
     4441 TTTTCCAATG ATGAGCACTT TTAAAGGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA
     4501 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA
     4561 ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA
     4621 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA
     4681 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT
     4741 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC
     4801 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA
     4861 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT
     4921 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA
     4981 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG
     5041 CGTCAGACCC CTTAATAAGA TGATCTTCTT GAGATCGTTT TGGTCTGCGC GTAATCTCTT
     5101 GCTCTGAAAA CGAAAAAACC GCCTTGCAGG GCGGTTTTTC GAAGGTTCTC TGAGCTACCA
     5161 ACTCTTTGAA CCGAGGTAAC TGGCTTGGAG GAGCGCAGTC ACCAAAACTT GTCCTTTCAG
     5221 TTTAGCCTTA ACC
//