LOCUS       pSEVA131-GG-grna-empty  4107 bp ds-DNA     circular     03-MAY-2023
DEFINITION  .
ACCESSION   JX560328
VERSION     JX560328.1
KEYWORDS    "Responsible person/group" "Usage" "Status" "Parent plasmids"
            "Cloning sites" "Antibiotic resistance" "Origin of replication"
            "Promoters" "RBS" "Genes" "Tags" "Markers" "Spacers" "gRNAs"
            "Terminators" "DNA fragments" "Protein" "Backbones" "Design entry"
            "LabCollector ID" "Comment" "Box" "Description" "Name"
SOURCE      Cloning vector pSEVA231
  ORGANISM  Cloning vector pSEVA231 other sequences; artificial sequences;
            vectors.
COMMENT     Imported using the Genbank importer. File name:
            /tmp/pSEVA_plasmids_genbank/pSEVA231.gb Imported using the Genbank
            importer. File name: /tmp/pSEVA_plasmids_genbank/pSEVA231.gb
            Imported using the Genbank importer. File name:
            /tmp/pSEVA_plasmids_genbank/pSEVA231.gb
FEATURES             Location/Qualifiers
     misc_feature    1383..1487
                     /label="T1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     1497..1520
                     /label="R24"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1533..1549)
                     /label="SEVA-GG-rev"
                     /note="sequence: aGGtCtCtgaaTtcgcgcggccgcg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(1572..1577)
                     /label="BsaIRec"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1572..1593
                     /label="GG-insert-for"
                     /note="sequence: attcaGAGACCTgcccattgacaaggc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    1572..2436
                     /label="insert"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1579..1619
                     /label="promoter 14g"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     source          1579..1619
                     /label="derived from cloning vector pSEVA211 as backbonetoclone mini-Tn7 fragments bearing Tn7extremes,multi-cloning site, and in some cases reporter(gfp) andsynthetic promoters"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1639..1646
                     /label="SD1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1652..1654
                     /label="Synthetic ATG"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_fwdcolor="#008080"
     misc_feature    complement(1679..1703)
                     /label="481_BCD2_rv"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1690..1698
                     /label="SD2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     gene            1705..2421
                     /label="msfGFP"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(2410..2436)
                     /label="GG-insert-rev"
                     /note="sequence: aGAGACCacTagtcatttatttgtagag"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2410..2437)
                     /label="GG-insert-rev"
                     /note="sequence: aGAGACCacTagtcatttatttgtagag"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2410..2438)
                     /label="GG-insert-repeat-rev"
                     /note="sequence: ACGAGACCactagtcatttatttgtagag"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2428..2484
                     /label="SEVA-repeat-GG-U-for"
                     /note="sequence: agTGGTCTCGtCGCcCggCaaAaccGgGCGTGGATTGAAACgcttgcggccgcgtcg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2431..2436
                     /label="BsaIRec"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2437..2468
                     /label="modified repeat"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2437..2468
                     /label="insert2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          2469..2480
                     /label="SEVA-GG-for"
                     /note="sequence: agtGGtCtCTgcaagcttgcggccgc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(2481..2504)
                     /label="F24"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2511..2613
                     /label="T0"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2774..3634
                     /label="Ap"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3700..3945
                     /label="oriT"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     gene            complement(3954..509)
                     /label="rep gene"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             complement(3954..509)
                     /label="rep CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 atgccgggct ggctgggcgg ctcctcgccg gggccggtcg gtagttgctg ctcgcccgga
       61 tacagggtcg ggatgcggcg caggtcgcca tgccccaaca gcgattcgtc ctggtcgtcg
      121 tgatcaacca ccacggcggc actgaacacc gacaggcgca actggtcgcg gggctggccc
      181 cacgccacgc ggtcattgac cacgtaggcc gacacggtgc cggggccgtt gagcttcacg
      241 acggagatcc agcgctcggc caccaagtcc ttgactgcgt attggaccgt ccgcaaagaa
      301 cgtccgatga gcttggaaag tgtcttctgg ctgaccacca cggcgttctg gtggcccatc
      361 tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt tcctcgcaat aagcccggcc
      421 cacgcctcat gcgctttgcg ttccgtttgc acccagtgac cgggcttgtt cttggcttga
      481 atgccgattt ctctggactg cgtggccatg cttatctcca tgcggtaggg gtgccgcacg
      541 gttgcggcac catgcgcaat cagctgcaac ttttcggcag cgcgacaaca attatgcgtt
      601 gcgtaaaagt ggcagtcaat tacagatttt ctttaaccta cgcaatgagc tattgcgggg
      661 ggtgccgcaa tgagctgttg cgtacccccc ttttttaagt tgttgatttt taagtctttc
      721 gcatttcgcc ctatatctag ttctttggtg cccaaagaag ggcacccctg cggggttccc
      781 ccacgccttc ggcgcggctc cccctccggc aaaaagtggc ccctccgggg cttgttgatc
      841 gactgcgcgg ccttcggcct tgcccaaggt ggcgctgccc ccttggaacc cccgcactcg
      901 ccgccgtgag gctcgggggg caggcgggcg ggcttcgccc ttcgactgcc cccactcgca
      961 taggcttggg tcgttccagg cgcgtcaagg ccaagccgct gcgcggtcgc tgcgcgagcc
     1021 ttgacccgcc ttccacttgg tgtccaaccg gcaagcgaag cgcgcaggcc gcaggccgga
     1081 ggcttttccc cagagaaaat taaaaaaatt gatggggcaa ggccgcaggc cgcgcagttg
     1141 gagccggtgg gtatgtggtc gaaggctggg tagccggtgg gcaatccctg tggtcaagct
     1201 cgtgggcagg cgcagcctgt ccatcagctt gtccagcagg gttgtccacg ggccgagcga
     1261 agcgagccag ccggtggccg ctcgcggcca tcgtccacat atccacgggc tggcaaggga
     1321 gcgcagcgac cgcgcagggc gaagcccgga gagcaagccc gtaggggggg cgcgcccagc
     1381 tgtctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     1441 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgccttt aattaaagcg
     1501 gataacaatt tcacacagga ggccgcctag gccgcggccg cgcgaattcg AGCTCGGTAC
     1561 CCGGGGATTC AGAGACCtgc ccattgacaa ggctctcgcg gccaggtata attgcacgag
     1621 ggcccaagtt cacttaaaaa ggagatcaac aatgaaagca attttcgtac tgaaacatct
     1681 taatcatgct aaggaggttt tctaatgcgt aaaggtgaag aactgttcac cggtgttgtt
     1741 ccgatcctgg ttgaactgga tggtgatgtt aacggccaca aattctctgt tcgtggtgaa
     1801 ggtgaaggtg atgcaaccaa cggtaaactg accctgaaat tcatctgcac taccggtaaa
     1861 ctgccggttc catggccgac tctggtgact accctgacct atggtgttca gtgtttttct
     1921 cgttacccgg atcacatgaa gcagcatgat ttcttcaaat ctgcaatgcc ggaaggttat
     1981 gtacaggagc gcaccatttc tttcaaagac gatggcacct acaaaacccg tgcagaggtt
     2041 aaatttgaag gtgatactct ggtgaaccgt attgaactga aaggcattga tttcaaagag
     2101 gacggcaaca tcctgggcca caaactggaa tataacttca actcccataa cgtttacatc
     2161 accgcagaca aacagaagaa cggtatcaaa gctaacttca aaattcgcca taacgttgaa
     2221 gacggtagcg tacagctggc ggaccactac cagcagaaca ctccgatcgg tgatggtccg
     2281 gttctgctgc cggataacca ctacctgtcc acccagtcta aactgtccaa agacccgaac
     2341 gaaaagcgcg accacatggt gctgctggag ttcgttactg ccgcaggtat cacgcacggc
     2401 atggatgaac tctacaaata aatgactagt GGTCTCGTCG CcCggCaaAa ccGgGCGTGG
     2461 ATTGAAACgc ttgcggccgc gtcgtgactg ggaaaaccct ggcgactagt cttggactcc
     2521 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
     2581 gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac gatttAAATT
     2641 AGTAGCCCGC CTAATGAGCG GGCTTTTTTT TAATTCCCCT ATTTGTTTAT TTTTCTAAAT
     2701 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG
     2761 AAAAAGGAAG AGTATGAGCA TTCAGCATTT TCGTGTGGCG CTGATTCCGT TTTTTGCGGC
     2821 GTTTTGCCTG CCGGTGTTTG CGCATCCGGA AACCCTGGTG AAAGTGAAAG ATGCGGAAGA
     2881 TCAACTGGGT GCGCGCGTGG GCTATATTGA ACTGGATCTG AACAGCGGCA AAATTCTGGA
     2941 ATCTTTTCGT CCGGAAGAAC GTTTTCCGAT GATGAGCACC TTTAAAGTGC TGCTGTGCGG
     3001 TGCGGTTCTG AGCCGTGTGG ATGCGGGCCA GGAACAACTG GGCCGTCGTA TTCATTATAG
     3061 CCAGAACGAT CTGGTGGAAT ATAGCCCGGT GACCGAAAAA CATCTGACCG ATGGCATGAC
     3121 CGTGCGTGAA CTGTGCAGCG CGGCGATTAC CATGAGCGAT AACACCGCGG CGAACCTGCT
     3181 GCTGACGACC ATTGGCGGTC CGAAAGAACT GACCGCGTTT CTGCATAACA TGGGCGATCA
     3241 TGTGACCCGT CTGGATCGTT GGGAACCGGA ACTGAACGAA GCGATTCCGA ACGATGAACG
     3301 TGATACCACC ATGCCGGCAG CAATGGCGAC CACCCTGCGT AAACTGCTGA CGGGTGAGCT
     3361 GCTGACCCTG GCAAGCCGCC AGCAACTGAT TGATTGGATG GAAGCGGATA AAGTGGCGGG
     3421 TCCGCTGCTG CGTAGCGCGC TGCCGGCTGG CTGGTTTATT GCGGATAAAA GCGGTGCGGG
     3481 CGAACGTGGC AGCCGTGGCA TTATTGCGGC GCTGGGCCCG GATGGTAAAC CGAGCCGTAT
     3541 TGTGGTGATT TATACCACCG GCAGCCAGGC GACGATGGAT GAACGTAACC GTCAGATTGC
     3601 GGAAATTGGC GCGAGCCTGA TTAAACATTG GTAAACCGAT ACAATTAAAG GCTCCTTTTG
     3661 GAGCCTTTTT TTTTGGACGA CCCcggtccg cgcgttgtcc ttttccgctg cataaccctg
     3721 cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg
     3781 attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag
     3841 gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca
     3901 cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgtaggccg gccctaccgg
     3961 cgcggcagcg ttacccgtgt cggcggctcc aacggctcgc catcgtccag aaaacacggc
     4021 tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc attcctccgt ttcggtcaag
     4081 gctggcaggt ctggttccat gcccgga
//