LOCUS       pSEVA231-GG-gRNA-empty  3995 bp ds-DNA     circular     03-MAY-2023
DEFINITION  .
ACCESSION   JX560328
VERSION     JX560328.1
KEYWORDS    "Responsible person/group" "Usage" "Status" "Parent plasmids"
            "Cloning sites" "Antibiotic resistance" "Origin of replication"
            "Promoters" "RBS" "Genes" "Tags" "Markers" "Spacers" "gRNAs"
            "Terminators" "DNA fragments" "Protein" "Backbones" "Design entry"
            "LabCollector ID" "Comment" "Box" "Description" "Name"
SOURCE      Cloning vector pSEVA231
  ORGANISM  Cloning vector pSEVA231 other sequences; artificial sequences;
            vectors.
COMMENT     Imported using the Genbank importer. File name:
            /tmp/pSEVA_plasmids_genbank/pSEVA231.gb Imported using the Genbank
            importer. File name: /tmp/pSEVA_plasmids_genbank/pSEVA231.gb
            Imported using the Genbank importer. File name:
            /tmp/pSEVA_plasmids_genbank/pSEVA231.gb
FEATURES             Location/Qualifiers
     misc_feature    1383..1487
                     /label="T1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     1497..1520
                     /label="R24"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1533..1549)
                     /label="SEVA-GG-rev"
                     /note="sequence: aGGtCtCtgaaTtcgcgcggccgcg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(1572..1577)
                     /label="BsaIRec"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1572..1593
                     /label="GG-insert-for"
                     /note="sequence: attcaGAGACCTgcccattgacaaggc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    1572..2436
                     /label="insert"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1579..1619
                     /label="promoter 14g"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     source          1579..1619
                     /label="derived from cloning vector pSEVA211 as backbonetoclone mini-Tn7 fragments bearing Tn7extremes,multi-cloning site, and in some cases reporter(gfp) andsynthetic promoters"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1639..1646
                     /label="SD1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1652..1654
                     /label="Synthetic ATG"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_fwdcolor="#008080"
     misc_feature    complement(1679..1703)
                     /label="481_BCD2_rv"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1690..1698
                     /label="SD2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     gene            1705..2421
                     /label="msfGFP"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(2410..2436)
                     /label="GG-insert-rev"
                     /note="sequence: aGAGACCacTagtcatttatttgtagag"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2410..2437)
                     /label="GG-insert-rev"
                     /note="sequence: aGAGACCacTagtcatttatttgtagag"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2410..2438)
                     /label="GG-insert-repeat-rev"
                     /note="sequence: ACGAGACCactagtcatttatttgtagag"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2428..2484
                     /label="SEVA-repeat-GG-U-for"
                     /note="sequence: agTGGTCTCGtCGCcCggCaaAaccGgGCGTGGATTGAAACgcttgcggccgcgtcg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2431..2436
                     /label="BsaIRec"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2437..2468
                     /label="modified repeat"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2437..2468
                     /label="insert2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          2469..2480
                     /label="SEVA-GG-for"
                     /note="sequence: agtGGtCtCTgcaagcttgcggccgc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(2481..2504)
                     /label="F24"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2511..2613
                     /label="T0"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2741..3556
                     /label="neo CDS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            2741..3556
                     /label="neo gene"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3588..3833
                     /label="oriT"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(3842..509)
                     /label="rep CDS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     gene            complement(3842..509)
                     /label="rep gene"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 atgccgggct ggctgggcgg ctcctcgccg gggccggtcg gtagttgctg ctcgcccgga
       61 tacagggtcg ggatgcggcg caggtcgcca tgccccaaca gcgattcgtc ctggtcgtcg
      121 tgatcaacca ccacggcggc actgaacacc gacaggcgca actggtcgcg gggctggccc
      181 cacgccacgc ggtcattgac cacgtaggcc gacacggtgc cggggccgtt gagcttcacg
      241 acggagatcc agcgctcggc caccaagtcc ttgactgcgt attggaccgt ccgcaaagaa
      301 cgtccgatga gcttggaaag tgtcttctgg ctgaccacca cggcgttctg gtggcccatc
      361 tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt tcctcgcaat aagcccggcc
      421 cacgcctcat gcgctttgcg ttccgtttgc acccagtgac cgggcttgtt cttggcttga
      481 atgccgattt ctctggactg cgtggccatg cttatctcca tgcggtaggg gtgccgcacg
      541 gttgcggcac catgcgcaat cagctgcaac ttttcggcag cgcgacaaca attatgcgtt
      601 gcgtaaaagt ggcagtcaat tacagatttt ctttaaccta cgcaatgagc tattgcgggg
      661 ggtgccgcaa tgagctgttg cgtacccccc ttttttaagt tgttgatttt taagtctttc
      721 gcatttcgcc ctatatctag ttctttggtg cccaaagaag ggcacccctg cggggttccc
      781 ccacgccttc ggcgcggctc cccctccggc aaaaagtggc ccctccgggg cttgttgatc
      841 gactgcgcgg ccttcggcct tgcccaaggt ggcgctgccc ccttggaacc cccgcactcg
      901 ccgccgtgag gctcgggggg caggcgggcg ggcttcgccc ttcgactgcc cccactcgca
      961 taggcttggg tcgttccagg cgcgtcaagg ccaagccgct gcgcggtcgc tgcgcgagcc
     1021 ttgacccgcc ttccacttgg tgtccaaccg gcaagcgaag cgcgcaggcc gcaggccgga
     1081 ggcttttccc cagagaaaat taaaaaaatt gatggggcaa ggccgcaggc cgcgcagttg
     1141 gagccggtgg gtatgtggtc gaaggctggg tagccggtgg gcaatccctg tggtcaagct
     1201 cgtgggcagg cgcagcctgt ccatcagctt gtccagcagg gttgtccacg ggccgagcga
     1261 agcgagccag ccggtggccg ctcgcggcca tcgtccacat atccacgggc tggcaaggga
     1321 gcgcagcgac cgcgcagggc gaagcccgga gagcaagccc gtaggggggg cgcgcccagc
     1381 tgtctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     1441 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgccttt aattaaagcg
     1501 gataacaatt tcacacagga ggccgcctag gccgcggccg cgcgaattcg AGCTCGGTAC
     1561 CCGGGGATTC AGAGACCtgc ccattgacaa ggctctcgcg gccaggtata attgcacgag
     1621 ggcccaagtt cacttaaaaa ggagatcaac aatgaaagca attttcgtac tgaaacatct
     1681 taatcatgct aaggaggttt tctaatgcgt aaaggtgaag aactgttcac cggtgttgtt
     1741 ccgatcctgg ttgaactgga tggtgatgtt aacggccaca aattctctgt tcgtggtgaa
     1801 ggtgaaggtg atgcaaccaa cggtaaactg accctgaaat tcatctgcac taccggtaaa
     1861 ctgccggttc catggccgac tctggtgact accctgacct atggtgttca gtgtttttct
     1921 cgttacccgg atcacatgaa gcagcatgat ttcttcaaat ctgcaatgcc ggaaggttat
     1981 gtacaggagc gcaccatttc tttcaaagac gatggcacct acaaaacccg tgcagaggtt
     2041 aaatttgaag gtgatactct ggtgaaccgt attgaactga aaggcattga tttcaaagag
     2101 gacggcaaca tcctgggcca caaactggaa tataacttca actcccataa cgtttacatc
     2161 accgcagaca aacagaagaa cggtatcaaa gctaacttca aaattcgcca taacgttgaa
     2221 gacggtagcg tacagctggc ggaccactac cagcagaaca ctccgatcgg tgatggtccg
     2281 gttctgctgc cggataacca ctacctgtcc acccagtcta aactgtccaa agacccgaac
     2341 gaaaagcgcg accacatggt gctgctggag ttcgttactg ccgcaggtat cacgcacggc
     2401 atggatgaac tctacaaata aatgactagt GGTCTCGTCG CcCggCaaAa ccGgGCGTGG
     2461 ATTGAAACgc ttgcggccgc gtcgtgactg ggaaaaccct ggcgactagt cttggactcc
     2521 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
     2581 gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac gatttaaatt
     2641 tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     2701 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcagcgtga
     2761 aacgagctgt agccgtccgc gtctgaacag caacatggat gcggatctgt atggctataa
     2821 atgggcgcgt gataacgtgg gtcagagcgg cgcgaccatt tatcgtctgt atggcaaacc
     2881 ggatgcgccg gaactgtttc tgaaacatgg caaaggcagc gtggcgaacg atgtgaccga
     2941 tgaaatggtg cgtctgaact ggctgaccga atttatgccg ctgccgacca ttaaacattt
     3001 tattcgcacc ccggatgatg cgtggctgct gaccaccgcg attccgggca aaaccgcgtt
     3061 tcaggtgctg gaagaatatc cggatagcgg cgaaaacatt gtggatgcgc tggccgtgtt
     3121 tctgcgtcgt ctgcatagca ttccggtgtg caactgcccg tttaacagcg atcgtgtgtt
     3181 tcgtctggcc caggcgcaga gccgtatgaa caacggcctg gtggatgcga gcgattttga
     3241 tgatgaacgt aacggctggc cggtggaaca ggtgtggaaa gaaatgcata aactgctgcc
     3301 gtttagcccg gatagcgtgg tgacccacgg cgattttagc ctggataacc tgattttcga
     3361 tgaaggcaaa ctgattggct gcattgatgt gggccgtgtg ggcattgcgg atcgttatca
     3421 ggatctggcc attctgtgga actgcctggg cgaatttagc ccgagcctgc aaaaacgtct
     3481 gtttcagaaa tatggcattg ataatccgga tatgaacaaa ctgcaatttc atctgatgct
     3541 ggatgaattt ttctaataat taattggacc gcggtccgcg cgttgtcctt ttccgctgca
     3601 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga
     3661 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag
     3721 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct
     3781 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaggccggc
     3841 cctaccggcg cggcagcgtt acccgtgtcg gcggctccaa cggctcgcca tcgtccagaa
     3901 aacacggctc atcgggcatc ggcaggcgct gctgcccgcg ccgttcccat tcctccgttt
     3961 cggtcaaggc tggcaggtct ggttccatgc ccgga
//