LOCUS       pSEVA431-GG-gRNA-empty  4054 bp ds-DNA     circular     03-MAY-2023
DEFINITION  .
ACCESSION   JX560338
VERSION     JX560338.2  GI:692118367
SOURCE      Cloning vector pSEVA431
  ORGANISM  Cloning vector pSEVA431 other sequences; artificial sequences;
            vectors.
COMMENT     Imported using the Genbank importer. File name: pSEVA431.gb Cloning
            vector pSEVA431, complete sequence. On Sep 30, 2014 this sequence
            version replaced JX560338.1. ##Assembly-Data-START## Assembly
            Method       :: ApE v. v2.0.40 Sequencing Technology :: Sanger
            dideoxy sequencing ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /label="source"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="organism: Cloning vector pSEVA431 mol_type: other DNA db_xref: taxon:1246742"
     primer_bind     9..32
                     /label="R24"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(45..61)
                     /label="SEVA-GG-rev"
                     /note="sequence: aGGtCtCtgaaTtcgcgcggccgcg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(84..89)
                     /label="BsaIRec"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          84..105
                     /label="GG-insert-for"
                     /note="sequence: attcaGAGACCTgcccattgacaaggc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    84..948
                     /label="insert"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    91..131
                     /label="promoter 14g"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     source          91..131
                     /label="derived from cloning vector pSEVA211 as backbonetoclone mini-Tn7 fragments bearing Tn7extremes,multi-cloning site, and in some cases reporter(gfp) andsynthetic promoters"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    151..158
                     /label="SD1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    164..166
                     /label="Synthetic ATG"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_fwdcolor="#008080"
     misc_feature    complement(191..215)
                     /label="481_BCD2_rv"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    202..210
                     /label="SD2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     gene            217..933
                     /label="msfGFP"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(922..948)
                     /label="GG-insert-rev"
                     /note="sequence: aGAGACCacTagtcatttatttgtagag"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(922..949)
                     /label="GG-insert-rev"
                     /note="sequence: aGAGACCacTagtcatttatttgtagag"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(922..950)
                     /label="GG-insert-repeat-rev"
                     /note="sequence: ACGAGACCactagtcatttatttgtagag"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          940..996
                     /label="SEVA-repeat-GG-U-for"
                     /note="sequence: agTGGTCTCGtCGCcCggCaaAaccGgGCGTGGATTGAAACgcttgcggccgcgtcg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    943..948
                     /label="BsaIRec"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    949..980
                     /label="insert2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    949..980
                     /label="modified repeat"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          981..992
                     /label="SEVA-GG-for"
                     /note="sequence: agtGGtCtCTgcaagcttgcggccgc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     complement(993..1016)
                     /label="F24"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1023..1125
                     /label="T0"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="regulatory_class: terminator"
     gene            1243..2148
                     /label="aadA gene"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             1243..2148
                     /label="aadA CDS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="transl_table: 11 product: spectinomycin adenyltransferase protein_id: AFV59796.1 db_xref: GI:410073708 translation: MPRASKQQARYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             1243..2148
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MPRASKQQARYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK*"
     misc_feature    2159..2404
                     /label="oriT"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(2413..3075)
                     /label="rep CDS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="transl_table: 11 product: replication protein protein_id: AFV59797.1 db_xref: GI:410073709 translation: MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLHHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     gene            complement(2413..3075)
                     /label="rep gene"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(2413..3075)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLHHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR*"
     misc_feature    3949..4053
                     /label="T1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="regulatory_class: terminator"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgAGCTCGGT ACCCGGGGAT TCAGAGACCt gcccattgac aaggctctcg cggccaggta
      121 taattgcacg agggcccaag ttcacttaaa aaggagatca acaatgaaag caattttcgt
      181 actgaaacat cttaatcatg ctaaggaggt tttctaatgc gtaaaggtga agaactgttc
      241 accggtgttg ttccgatcct ggttgaactg gatggtgatg ttaacggcca caaattctct
      301 gttcgtggtg aaggtgaagg tgatgcaacc aacggtaaac tgaccctgaa attcatctgc
      361 actaccggta aactgccggt tccatggccg actctggtga ctaccctgac ctatggtgtt
      421 cagtgttttt ctcgttaccc ggatcacatg aagcagcatg atttcttcaa atctgcaatg
      481 ccggaaggtt atgtacagga gcgcaccatt tctttcaaag acgatggcac ctacaaaacc
      541 cgtgcagagg ttaaatttga aggtgatact ctggtgaacc gtattgaact gaaaggcatt
      601 gatttcaaag aggacggcaa catcctgggc cacaaactgg aatataactt caactcccat
      661 aacgtttaca tcaccgcaga caaacagaag aacggtatca aagctaactt caaaattcgc
      721 cataacgttg aagacggtag cgtacagctg gcggaccact accagcagaa cactccgatc
      781 ggtgatggtc cggttctgct gccggataac cactacctgt ccacccagtc taaactgtcc
      841 aaagacccga acgaaaagcg cgaccacatg gtgctgctgg agttcgttac tgccgcaggt
      901 atcacgcacg gcatggatga actctacaaa taaatgacta gtGGTCTCGT CGCcCggCaa
      961 AaccGgGCGT GGATTGAAAC gcttgcggcc gcgtcgtgac tgggaaaacc ctggcgacta
     1021 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
     1081 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt
     1141 acgatttacg tatttaaatg aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     1201 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     1261 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     1321 cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa
     1381 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     1441 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     1501 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     1561 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     1621 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     1681 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     1741 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     1801 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     1861 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     1921 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     1981 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     2041 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     2101 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataaga caattgtcct
     2161 tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct
     2221 ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc
     2281 aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct
     2341 tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc
     2401 cgtaggccgg ccctaccggc gcggcagcgt tacccgtgtc ggcggctcca acggctcgcc
     2461 atcgtccaga aaacacggct catcgggcat cggcaggcgc tgctgcccgc gccgttccca
     2521 ttcctccgtt tcggtcaagg ctggcaggtc tggttccatg cccggaatgc cgggctggct
     2581 gggcggctcc tcgccggggc cggtcggtag ttgctgctcg cccggataca gggtcgggat
     2641 gcggcgcagg tcgccatgcc ccaacagcga ttcgtcctgg tcgtcgtgat caaccaccac
     2701 ggcggcactg aacaccgaca ggcgcaactg gtcgcggggc tggccccacg ccacgcggtc
     2761 attgaccacg taggccgaca cggtgccggg gccgttgagc ttcacgacgg agatccagcg
     2821 ctcggccacc aagtccttga ctgcgtattg gaccgtccgc aaagaacgtc cgatgagctt
     2881 ggaaagtgtc ttctggctga ccaccacggc gttctggtgg cccatctgcg ccacgaggtg
     2941 atgcagcagc attgccgccg tgggtttcct cgcaataagc ccggcccacg cctcatgcgc
     3001 tttgcgttcc gtttgcaccc agtgaccggg cttgttcttg gcttgaatgc cgatttctct
     3061 ggactgcgtg gccatgctta tctccatgcg gtaggggtgc cgcacggttg cggcaccatg
     3121 cgcaatcagc tgcaactttt cggcagcgcg acaacaatta tgcgttgcgt aaaagtggca
     3181 gtcaattaca gattttcttt aacctacgca atgagctatt gcggggggtg ccgcaatgag
     3241 ctgttgcgta cccccctttt ttaagttgtt gatttttaag tctttcgcat ttcgccctat
     3301 atctagttct ttggtgccca aagaagggca cccctgcggg gttcccccac gccttcggcg
     3361 cggctccccc tccggcaaaa agtggcccct ccggggcttg ttgatcgact gcgcggcctt
     3421 cggccttgcc caaggtggcg ctgccccctt ggaacccccg cactcgccgc cgtgaggctc
     3481 ggggggcagg cgggcgggct tcgcccttcg actgccccca ctcgcatagg cttgggtcgt
     3541 tccaggcgcg tcaaggccaa gccgctgcgc ggtcgctgcg cgagccttga cccgccttcc
     3601 acttggtgtc caaccggcaa gcgaagcgcg caggccgcag gccggaggct tttccccaga
     3661 gaaaattaaa aaaattgatg gggcaaggcc gcaggccgcg cagttggagc cggtgggtat
     3721 gtggtcgaag gctgggtagc cggtgggcaa tccctgtggt caagctcgtg ggcaggcgca
     3781 gcctgtccat cagcttgtcc agcagggttg tccacgggcc gagcgaagcg agccagccgg
     3841 tggccgctcg cggccatcgt ccacatatcc acgggctggc aagggagcgc agcgaccgcg
     3901 cagggcgaag cccggagagc aagcccgtag ggggggcgcg cccagctgtc tagggcggcg
     3961 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     4021 ctttcgactg agcctttcgt tttatttgat gcct
//