LOCUS       Exported                6782 bp ds-DNA     circular SYN 24-FEB-2023
DEFINITION  synthetic circular DNA.
ACCESSION   urn.local...34muarj-4v
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 26, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/locus_tag=source:synthetic DNA construct"
     primer_bind     64..80
                     /locus_tag="T3"
                     /label=T3
     promoter        105..707
                     /locus_tag="Bidirectional promoter"
                     /label=Bidirectional promoter
     5'UTR           complement(105..112)
                     /locus_tag="Partial"
                     /label=Partial
     promoter        complement(112..470)
                     /locus_tag="promoter"
                     /label=promoter
     misc_feature    112
                     /locus_tag="Geneious type: misc_marker"
                     /label=Geneious type: misc_marker
     promoter        496..707
                     /locus_tag="GAL1 minimal promoter"
                     /label=GAL1 minimal promoter
     primer_bind     648..675
                     /locus_tag="TNS_o64"
                     /label=TNS_o64
     5'UTR           707..763
                     /locus_tag="Partial (all but the last 4 bases)"
                     /label=Partial (all but the last 4 bases)
     misc_feature    707
                     /locus_tag="Geneious type: misc_marker(1)"
                     /label=Geneious type: misc_marker(1)
     primer_bind     727..750
                     /locus_tag="Gal1seq"
                     /label=Gal1seq
     primer_bind     727..750
                     /locus_tag="TNS_o29"
                     /label=TNS_o29
     misc_feature    772..777
                     /locus_tag="EcoRI"
                     /label=EcoRI
     5'UTR           779..802
                     /locus_tag="5'UTR"
                     /label=5'UTR
     CDS             803..1063
                     /locus_tag="CDS (Aga2p)"
                     /label=CDS (Aga2p)
     misc_feature    1065..1089
                     /locus_tag="primer: o4 Aga2p_fwd"
                     /label=primer: o4 Aga2p_fwd
     CDS             1097..1132
                     /locus_tag="CDS (HA tag)"
                     /label=CDS (HA tag)
     primer_bind     1098..1118
                     /locus_tag="oTNS_065"
                     /label=oTNS_065
     misc_feature    1120..1189
                     /locus_tag="Twist 5' overlap"
                     /label=Twist 5' overlap
     primer_bind     1120..1139
                     /locus_tag="o232"
                     /label=o232
     CDS             1133..1177
                     /locus_tag="CDS (GGS linker)"
                     /label=CDS (GGS linker)
     misc_feature    1155..1162
                     /locus_tag="NotI"
                     /label=NotI
     primer_bind     1169..1189
                     /locus_tag="oTNS_005"
                     /label=oTNS_005
     misc_binding    1184..1189
                     /locus_tag="NdeI"
                     /label=NdeI
     misc_feature    1190..1792
                     /locus_tag="RBD Omicron XBB.1.5"
                     /label=RBD Omicron XBB.1.5
     misc_feature    1214..1216
                     /locus_tag="D339H"
                     /label=D339H
     misc_feature    1235..1237
                     /locus_tag="R346T"
                     /label=R346T
     misc_feature    1301..1303
                     /locus_tag="L368I"
                     /label=L368I
     misc_feature    1532..1534
                     /locus_tag="V445P"
                     /label=V445P
     misc_feature    1535..1537
                     /locus_tag="G446S"
                     /label=G446S
     misc_feature    1577..1579
                     /locus_tag="N460K"
                     /label=N460K
     misc_feature    1655..1657
                     /locus_tag="F486P"
                     /label=F486P
     misc_feature    1667..1669
                     /locus_tag="F490S"
                     /label=F490S
     misc_feature    1676..1678
                     /locus_tag="R493Q"
                     /label=R493Q
     primer_bind     complement(1793..1812)
                     /locus_tag="TNS_o6"
                     /label=TNS_o6
     misc_binding    1793..1798
                     /locus_tag="XhoI"
                     /label=XhoI
     CDS             1799..1810
                     /locus_tag="CDS"
                     /label=CDS
     CDS             1811..1840
                     /locus_tag="CDS (Myc tag)"
                     /label=CDS (Myc tag)
     3'UTR           1851..2155
                     /locus_tag="yeast mating factor alpha 3' UTR"
                     /label=yeast mating factor alpha 3' UTR
     primer_bind     1881..1910
                     /locus_tag="AG_211"
                     /label=AG_211
     primer_bind     complement(1881..1910)
                     /locus_tag="AG_212"
                     /label=AG_212
     misc_feature    2156..2161
                     /locus_tag="SacI"
                     /label=SacI
     primer_bind     complement(2169..2188)
                     /locus_tag="T7"
                     /label=T7
     misc_feature    2208..2241
                     /locus_tag="Illumina P7 handle"
                     /label=Illumina P7 handle
     rep_origin      2386..2841
                     /locus_tag="rep_origin"
                     /label=rep_origin
     misc_feature    2511..2516
                     /locus_tag="NaeI"
                     /label=NaeI
     3'UTR           complement(2898..2943)
                     /locus_tag="3'UTR"
                     /label=3'UTR
     CDS             complement(2944..3618)
                     /locus_tag="Tyrosinase-related protein 1 (YeastSelectable
                     marker)"
                     /label=Tyrosinase-related protein 1
                     (YeastSelectable m...
                     /label=Tyrosinase-related protein 1 (YeastSelectable
                     marker)
     5'UTR           complement(3619..3722)
                     /locus_tag="5'UTR(1)"
                     /label=5'UTR(1)
     rep_origin      complement(4151..4664)
                     /direction=LEFT
                     /locus_tag="rep_origin(1)"
                     /label=rep_origin(1)
     rep_origin      complement(4155..4531)
                     /direction=LEFT
                     /locus_tag="rep_origin(2)"
                     /label=rep_origin(2)
     centromere      4544..4569
                     /label=CDEI fragment of chromosome VI
                     centromerefrom  ...
                     /label=CDEI fragment of chromosome VI centromerefrom 
                     S.cerevisiae
                     /note="/locus_tag=CDEI fragment of chromosome VI
                     centromerefrom S.cerevisiae"
                     /note="/locus_tag=CDEI fragment of chromosome VI 
                     centromerefrom S.cerevisiae"
     promoter        4733..4761
                     /locus_tag="promoter(1)"
                     /label=promoter(1)
     CDS             4803..5663
                     /locus_tag="CDS(1)"
                     /label=CDS(1)
     rep_origin      5811..6596
                     /locus_tag="rep_origin(3)"
                     /label=rep_origin(3)
ORIGIN
        1 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
       61 gaaattaacc ctcactaaag ggaacaaaag ctggtaccaa ttccttgaat tttcaaaaat
      121 tcttactttt tttttggatg gacgcaaaga agtttaataa tcatattaca tggcattacc
      181 accatataca tatccatatc taatcttact tatatgttgt ggaaatgtaa agagccccat
      241 tatcttagcc taaaaaaacc ttctctttgg aactttcagt aatacgctta actgctcatt
      301 gctatattga agtacggatt agaagccgcc gagcgggtga cagccctccg aaggaagact
      361 ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc
      421 cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat gaagaggaaa
      481 aattggcagt aacctggccc cacaaacctt caaatgaacg aatcaaatta acaaccatag
      541 gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag cgaagcgatg
      601 atttttgatc tattaacaga tatataaatg caaaaactgc ataaccactt taactaatac
      661 tttcaacatt ttcggtttgt attacttctt attcaaatgt aataaaagta tcaacaaaaa
      721 attgttaata tacctctata ctttaacgtc aaggagaaaa aaccccggat cgaattccct
      781 acttcataca ttttcaatta agatgcagtt acttcgctgt ttttcaatat tttctgttat
      841 tgcttcagtt ttagcacagg aactgacaac tatatgcgag caaatcccct caccaacttt
      901 agaatcgacg ccgtactctt tgtcaacgac tactattttg gccaacggga aggcaatgca
      961 aggagttttt gaatattaca aatcagtaac gtttgtcagt aattgcggtt ctcacccctc
     1021 aacaactagc aaaggcagcc ccataaacac acagtatgtt tttaaggaca atagctcgac
     1081 gattgaaggt agatacccat acgacgttcc agactacgct ctgcaggcta gtggtggagg
     1141 aggctctggt ggaggcggcc gcggaggcgg agggtcggct agccatatga acatcaccaa
     1201 cttgtgtcca ttccatgaag ttttcaatgc tactactttc gcttctgttt acgcttggaa
     1261 tagaaagaga atctctaact gcgttgctga ctattctgtc atttacaatt ttgctccatt
     1321 cttcgctttc aagtgctatg gtgtttctcc aactaagttg aacgatttgt gtttcaccaa
     1381 cgtttacgcc gattcctttg ttattagagg taacgaagtc tcccaaattg ctccaggtca
     1441 aactggtaat attgccgatt acaattacaa gttgccagat gatttcaccg gttgtgttat
     1501 tgcttggaac tctaacaagt tggattctaa gccttctggc aactacaatt acttgtacag
     1561 gttgttccgt aagtccaaat tgaagccatt cgaaagagat atttccaccg aaatctatca
     1621 agctggtaac aagccatgta atggtgttgc tggtcctaac tgttactctc cattgcaatc
     1681 ttacggtttc agaccaactt atggtgttgg tcatcaacca tacagagttg ttgttttgtc
     1741 tttcgagttg ttgcatgctc cagctactgt ttgtggtcca aagaaatcta ctctcgaggg
     1801 gggcggttcc gaacaaaagc ttatttctga agaggacttg taatagagat ctgataacaa
     1861 cagtgtagat gtaacaaaat cgactttgtt cccactgtac ttttagctcg tacaaaatac
     1921 aatatacttt tcatttctcc gtaaacaaca tgttttccca tgtaatatcc ttttctattt
     1981 ttcgttccgt taccaacttt acacatactt tatatagcta ttcacttcta tacactaaaa
     2041 aactaagaca attttaattt tgctgcctgc catatttcaa tttgttataa attcctataa
     2101 tttatcctat tagtagctaa aaaaagatga atgtgaatcg aatcctaaga gaattgagct
     2161 ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgttaga tcggaagagc
     2221 acacgtctga actccagtca cttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     2281 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     2341 accgatcgcc tttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg
     2401 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2461 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2521 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2581 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     2641 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2701 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     2761 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     2821 aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2881 ttcacaccgc atagatcggc aagtgcacaa acaatactta aataaatact actcagtaat
     2941 aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt ttacaccatt
     3001 tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa gcgcatcacc
     3061 aacattttct ggcgtcagtc caccagctaa cataaaatgt aagctttcgg ggctctcttg
     3121 ccttccaacc cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc cacctgcttc
     3181 tgaatcaaac aagggaataa acgaatgagg tttctgtgaa gctgcactga gtagtatgtt
     3241 gcagtctttt ggaaatacga gtcttttaat aactggcaaa ccgaggaact cttggtattc
     3301 ttgccacgac tcatctccat gcagttggac gatatcaatg ccgtaatcat tgaccagagc
     3361 caaaacatcc tccttaggtt gattacgaaa cacgccaacc aagtatttcg gagtgcctga
     3421 actattttta tatgctttta caagacttga aattttcctt gcaataaccg ggtcaattgt
     3481 tctctttcta ttgggcacac atataatacc cagcaagtca gcatcggaat ctagagcaca
     3541 ttctgcggcc tctgtgctct gcaagccgca aactttcacc aatggaccag aactacctgt
     3601 gaaattaata acagacatac tccaagctgc ctttgtgtgc ttaatcacgt atactcacgt
     3661 gctcaatagt caccaatgcc ctccctcttg gccctctcct tttctttttt cgaccgaatt
     3721 aattcttaat cggcaaaaaa agaaaagctc cggatcaaga ttgtacgtaa ggtgacaagc
     3781 tatttttcaa taaagaatat cttccactac tgccatctgg cgtcataact gcaaagtaca
     3841 catatattac gatgctgttc tattaaatgc ttcctatatt atatatatag taatgtcgtg
     3901 atctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     3961 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     4021 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     4081 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4141 taatggtttc ttagacggat cgcttgcctg taacttacac gcgcctcgta tcttttaatg
     4201 atggaataat ttgggaattt actctgtgtt tatttatttt tatgttttgt atttggattt
     4261 tagaaagtaa ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa taaacaaagg
     4321 tttaaaaaat ttcaacaaaa agcgtacttt acatatatat ttattagaca agaaaagcag
     4381 attaaataga tatacattcg attaacgata agtaaaatgt aaaatcacag gattttcgtg
     4441 tgtggtcttc tacacagaca agatgaaaca attcggcatt aatacctgag agcaggaaga
     4501 gcaagataaa aggtagtatt tgttggcgat ccccctagag tcttttacat cttcggaaaa
     4561 caaaaactat tttttcttta atttcttttt ttactttcta tttttaattt atatatttat
     4621 attaaaaaat ttaaattata attattttta tagcacgtga tgaaaaggac ccaggtggca
     4681 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4741 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4801 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4861 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4921 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4981 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5041 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5101 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5161 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5221 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5281 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5341 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5401 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5461 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5521 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5581 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5641 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5701 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5761 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5821 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5881 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5941 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6001 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6061 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6121 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6181 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6241 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6301 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6361 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6421 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6481 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6541 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6601 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     6661 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     6721 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     6781 ga
//
