LOCUS       Exported                9556 bp DNA     circular SYN 23-AUG-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 23, 2023 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9556
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_structure  2028..2151
                     /label=cPPT
     misc_feature    2028..2144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     primer_bind     2161..2204
                     /label=hPGK_ClaI.FOR
     promoter        2185..2700
                     /label=human PGK promoter
     primer_bind     2326..2342
                     /label=hPGK_Colony.FOR
     primer_bind     2619..2637
                     /label=hPGK_end_seq.FOR
     primer_bind     complement(2668..2685)
                     /label=hPGK_Colony.REV
     primer_bind     2685..2739
                     /label=LTB4R-frag-F
     primer_bind     2706..2744
                     /label=LTB4R-APEX2 Gibson fwd
                     /note="Binding region: 28 nt, 50% GC, Tm: 73 (o)C"
     primer_bind     2716..2740
                     /label=LTB4_AscI_frag_F
     CDS             2718..3773
                     /codon_start=1
                     /label=LTB4R
                     /translation="MNTTSSAAPPSLGVEFISLLAIILLSVALAVGLPGNSFVVWSILK
                     RMQKRSVTALMVLNLALADLAVLLTAPFFLHFLAQGTWSFGLAGCRLCHYVCGVSMYAS
                     VLLITAMSLDRSLAVARPFVSQKLRTKAMARRVLAGIWVLSFLLATPVLAYRTVVPWKT
                     NMSLCFPRYPSEGHRAFHLIFEAVTGFLLPFLAVVASYSDIGRRLQARRFRRSRRTGRL
                     VVLIILTFAAFWLPYHVVNLAEAGRALAGQAAGLGLVGKRLSLARNVLIALAFLSSSVN
                     PVLYACAGGGLLRSAGVGFVAKLLEGTGAEASSTRRGGSLGQTARSGPAALEPGPSESL
                     TASSPLKLNELN"
     primer_bind     2928..2948
                     /label=LTB4R Col PCR fwd
                     /note="Taq polymerase: 21 nt, 48% GC, Tm: 55 (o)C480 bp PCR
                     product"
     primer_bind     complement(3389..3407)
                     /label=LTB4R Col PCR rev
                     /note="19 nt, 53% GC, Tm: 54 (o)C480 bp PCR product"
     primer_bind     complement(3449..3459)
                     /label=Hygro.REV
     primer_bind     complement(3623..3644)
                     /label=LTB4_T308A_bottom
     primer_bind     complement(3630..3658)
                     /label=LTB4_T308A_S310A_R
     primer_bind     3630..3658
                     /label=LTB4_T308A_S310A_F
     primer_bind     3632..3655
                     /label=LTB4_T308A_top
     primer_bind     complement(3636..3656)
                     /label=LTB4_S310A_R
     primer_bind     3636..3656
                     /label=LTB4_S310A_F
     CDS             3639..3641
                     /codon_start=1
                     /label=T308 phos site
                     /translation="T"
     CDS             3646..3647
                     /codon_start=1
                     /label=S310 phos site
                     /translation=""
     primer_bind     complement(3743..3773)
                     /label=LTB4R-APEX2 Gibson rev
                     /note="Binding region: 30 nt, 47% GC, Tm: 73 (o)C"
     primer_bind     complement(3746..3779)
                     /label=LTB4_MluI_frag_R
     primer_bind     complement(3755..3800)
                     /label=LTB4R-frag-R
     primer_bind     3759..3783
                     /label=LAMP1_NotI_frag_FOR
     primer_bind     3777..3796
                     /label=SC160_vect_F
     misc_feature    3814..4425
                     /label=IRES
     primer_bind     complement(3949..3968)
                     /label=IRES_5'-Seq_R
     primer_bind     4331..4350
                     /label=IRES_end_seq.FOR
     primer_bind     4380..4394
                     /label=IRES_BmgBI_Hygro.FOR
     CDS             4429..5454
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     4524..4542
                     /label=Hygro_Col.FOR
     primer_bind     complement(4918..4935)
                     /label=Hygro_Col.REV
     primer_bind     complement(5434..5475)
                     /label=Hygro_Xma.REV
     primer_bind     complement(5459..5475)
                     /label=Hygro.REV
     misc_feature    5478..6069
                     /label=WPRE
     misc_feature    5478..6066
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
     primer_bind     complement(5627..5646)
                     /label=WPRE_seq.R
     CDS             complement(5949..5960)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5979..6035
                     /label=Hygro_Xma.REV
     LTR             6274..6907
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7036..7052)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7060..7076
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7084..7114)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7129..7150
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7438..8023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8194..9054)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9055..9159)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9207..9341
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatccacgg ggttggggtt gcgccttttc caaggcagcc
     2221 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
     2281 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
     2341 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
     2401 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
     2461 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
     2521 gctgctcagc ggggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
     2581 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
     2641 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg
     2701 acgcgggatc cgccaccatg aacactacat cttctgcagc acccccctca ctaggtgtag
     2761 agttcatctc tctgctggct atcatcctgc tgtcagtggc gctggctgtg gggcttcccg
     2821 gcaacagctt tgtggtgtgg agtatcctga aaaggatgca gaagcgctct gtcactgccc
     2881 tgatggtgct gaacctggcc ctggccgacc tggccgtatt gctcactgct ccctttttcc
     2941 ttcacttcct ggcccaaggc acctggagtt ttggactggc tggttgccgc ctgtgtcact
     3001 atgtctgcgg agtcagcatg tacgccagcg tcctgcttat cacggccatg agtctagacc
     3061 gctcactggc ggtggcccgc ccctttgtgt cccagaagct acgcaccaag gcgatggccc
     3121 ggcgggtgct ggcaggcatc tgggtgttgt cctttctgct ggccacaccc gtcctcgcgt
     3181 accgcacagt agtgccctgg aaaacgaaca tgagcctgtg cttcccgcgg taccccagcg
     3241 aagggcaccg ggccttccat ctaatcttcg aggctgtcac gggcttcctg ctgcccttcc
     3301 tggctgtggt ggccagctac tcggacatag ggcgtcggct acaggcccgg cgcttccgcc
     3361 gcagccgccg caccggccgc ctggtggtgc tcatcatcct gaccttcgcc gccttctggc
     3421 tgccctacca cgtggtgaac ctggctgagg cgggccgcgc gctggccggc caggccgccg
     3481 ggttagggct cgtggggaag cggctgagcc tggcccgcaa cgtgctcatc gcactcgcct
     3541 tcctgagcag cagcgtgaac cccgtgctgt acgcgtgcgc cggcggcggc ctgctgcgct
     3601 cggcgggcgt gggcttcgtc gccaagctgc tggagggcac gggcgccgag gcgtccagca
     3661 cgcgccgcgg gggcagcctg ggccagaccg ctaggagcgg ccccgccgct ctggagcccg
     3721 gcccttccga gagcctcact gcctccagcc ctctcaagtt aaacgaactg aactaagcgg
     3781 ccgctctaga gtcgacgggc cgcggtaaca attgttaact aacttaagct agcaacggtt
     3841 tccctctagc gggatcaatt ccgccccccc cccctaacgt tactggccga agccgcttgg
     3901 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     3961 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     4021 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     4081 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     4141 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     4201 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     4261 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     4321 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     4381 ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat gaaaaagcct
     4441 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     4501 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     4561 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     4621 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     4681 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     4741 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     4801 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     4861 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     4921 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     4981 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     5041 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     5101 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     5161 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     5221 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     5281 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     5341 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     5401 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcccggg
     5461 cggggcgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5521 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5581 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5641 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5701 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5761 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5821 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     5881 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5941 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6001 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6061 ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag
     6121 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     6181 gggtttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     6241 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa
     6301 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     6361 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     6421 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     6481 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     6541 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     6601 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     6661 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     6721 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6781 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6841 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6901 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     6961 atatcagaga gtgagaggcc ttgacattgc tagcgtttta ccgtcgacct ctagctagag
     7021 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7081 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7141 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7201 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7261 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7321 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7381 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7441 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7501 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7561 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7621 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7681 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7741 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7801 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7861 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7921 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt
     7981 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8041 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8101 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8161 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8221 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8281 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8341 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8401 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8461 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8521 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8581 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8641 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8701 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8761 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8821 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8881 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8941 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9001 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9061 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9121 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9181 acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat
     9241 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9301 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
     9361 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctagtgg
     9421 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
     9481 tgttaaatat gtactacaaa cttagtagtt tttaaagaaa ttgtatttgt taaatatgta
     9541 ctacaaactt agtagt
//
