LOCUS       Exported                9637 bp DNA     circular SYN 23-AUG-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 23, 2023 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_structure  2028..2151
                     /label=cPPT
     misc_feature    2028..2144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     primer_bind     2161..2204
                     /label=hPGK_ClaI.FOR
     promoter        2185..2700
                     /label=human PGK promoter
     primer_bind     2326..2342
                     /label=hPGK_Colony.FOR
     primer_bind     2619..2637
                     /label=hPGK_end_seq.FOR
     primer_bind     complement(2668..2685)
                     /label=hPGK_Colony.REV
     primer_bind     2685..2823
                     /label=oEA82
     CDS             2718..2720
                     /codon_start=1
                     /label=Start
                     /translation="M"
     CDS             2721..2801
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             2802..3854
                     /codon_start=1
                     /label=LTB4R
                     /translation="NTTSSAAPPSLGVEFISLLAIILLSVALAVGLPGNSFVVWSILKR
                     MQKRSVTALMVLNLALADLAVLLTAPFFLHFLAQGTWSFGLAGCRLCHYVCGVSMYASV
                     LLITAMSLDRSLAVARPFVSQKLRTKAMARRVLAGIWVLSFLLATPVLAYRTVVPWKTN
                     MSLCFPRYPSEGHRAFHLIFEAVTGFLLPFLAVVASYSDIGRRLQARRFRRSRRTGRLV
                     VLIILTFAAFWLPYHVVNLAEAGRALAGQAAGLGLVGKRLSLARNVLIALAFLSSSVNP
                     VLYACAGGGLLRSAGVGFVAKLLEGTGAEASSTRRGGSLGQTARSGPAALEPGPSESLT
                     ASSPLKLNELN"
     primer_bind     3009..3029
                     /label=LTB4R Col PCR fwd
                     /note="Taq polymerase: 21 nt, 48% GC, Tm: 55 (o)C480 bp PCR
                     product"
     primer_bind     complement(3470..3488)
                     /label=LTB4R Col PCR rev
                     /note="19 nt, 53% GC, Tm: 54 (o)C480 bp PCR product"
     primer_bind     complement(3530..3540)
                     /label=Hygro.REV
     primer_bind     complement(3704..3725)
                     /label=LTB4_T308A_bottom
     primer_bind     complement(3711..3739)
                     /label=LTB4_T308A_S310A_R
     primer_bind     3711..3739
                     /label=LTB4_T308A_S310A_F
     primer_bind     3713..3736
                     /label=LTB4_T308A_top
     primer_bind     complement(3717..3737)
                     /label=LTB4_S310A_R
     primer_bind     3717..3737
                     /label=LTB4_S310A_F
     CDS             3720..3722
                     /codon_start=1
                     /label=T308 phos site
                     /translation="T"
     CDS             3727..3728
                     /codon_start=1
                     /label=S310 phos site
                     /translation=""
     primer_bind     complement(3824..3854)
                     /label=LTB4R-APEX2 Gibson rev
                     /note="Binding region: 30 nt, 47% GC, Tm: 73 (o)C"
     primer_bind     complement(3827..3860)
                     /label=LTB4_MluI_frag_R
     primer_bind     complement(3836..3881)
                     /label=LTB4R-frag-R
     primer_bind     3840..3864
                     /label=LAMP1_NotI_frag_FOR
     primer_bind     3858..3877
                     /label=SC160_vect_F
     misc_feature    3895..4506
                     /label=IRES
     primer_bind     complement(4030..4049)
                     /label=IRES_5'-Seq_R
     primer_bind     4412..4431
                     /label=IRES_end_seq.FOR
     primer_bind     4461..4475
                     /label=IRES_BmgBI_Hygro.FOR
     CDS             4510..5535
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     4605..4623
                     /label=Hygro_Col.FOR
     primer_bind     complement(4999..5016)
                     /label=Hygro_Col.REV
     primer_bind     complement(5515..5556)
                     /label=Hygro_Xma.REV
     primer_bind     complement(5540..5556)
                     /label=Hygro.REV
     misc_feature    5559..6150
                     /label=WPRE
     misc_feature    5559..6147
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
     primer_bind     complement(5708..5727)
                     /label=WPRE_seq.R
     CDS             complement(6030..6041)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6060..6116
                     /label=Hygro_Xma.REV
     LTR             6355..6988
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7117..7133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7141..7157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7165..7195)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7210..7231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7519..8104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8275..9135)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9136..9240)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9288..9422
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatccacgg ggttggggtt gcgccttttc caaggcagcc
     2221 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
     2281 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
     2341 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
     2401 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
     2461 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
     2521 gctgctcagc ggggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
     2581 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
     2641 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg
     2701 acgcgggatc cgccaccatg tacccatacg atgttccaga ttacgcttac ccatacgatg
     2761 ttccagatta cgcttaccca tacgatgttc cagattacgc taacactaca tcttctgcag
     2821 cacccccctc actaggtgta gagttcatct ctctgctggc tatcatcctg ctgtcagtgg
     2881 cgctggctgt ggggcttccc ggcaacagct ttgtggtgtg gagtatcctg aaaaggatgc
     2941 agaagcgctc tgtcactgcc ctgatggtgc tgaacctggc cctggccgac ctggccgtat
     3001 tgctcactgc tccctttttc cttcacttcc tggcccaagg cacctggagt tttggactgg
     3061 ctggttgccg cctgtgtcac tatgtctgcg gagtcagcat gtacgccagc gtcctgctta
     3121 tcacggccat gagtctagac cgctcactgg cggtggcccg cccctttgtg tcccagaagc
     3181 tacgcaccaa ggcgatggcc cggcgggtgc tggcaggcat ctgggtgttg tcctttctgc
     3241 tggccacacc cgtcctcgcg taccgcacag tagtgccctg gaaaacgaac atgagcctgt
     3301 gcttcccgcg gtaccccagc gaagggcacc gggccttcca tctaatcttc gaggctgtca
     3361 cgggcttcct gctgcccttc ctggctgtgg tggccagcta ctcggacata gggcgtcggc
     3421 tacaggcccg gcgcttccgc cgcagccgcc gcaccggccg cctggtggtg ctcatcatcc
     3481 tgaccttcgc cgccttctgg ctgccctacc acgtggtgaa cctggctgag gcgggccgcg
     3541 cgctggccgg ccaggccgcc gggttagggc tcgtggggaa gcggctgagc ctggcccgca
     3601 acgtgctcat cgcactcgcc ttcctgagca gcagcgtgaa ccccgtgctg tacgcgtgcg
     3661 ccggcggcgg cctgctgcgc tcggcgggcg tgggcttcgt cgccaagctg ctggagggca
     3721 cgggcgccga ggcgtccagc acgcgccgcg ggggcagcct gggccagacc gctaggagcg
     3781 gccccgccgc tctggagccc ggcccttccg agagcctcac tgcctccagc cctctcaagt
     3841 taaacgaact gaactaagcg gccgctctag agtcgacggg ccgcggtaac aattgttaac
     3901 taacttaagc tagcaacggt ttccctctag cgggatcaat tccgcccccc ccccctaacg
     3961 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     4021 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     4081 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     4141 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     4201 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     4261 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     4321 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     4381 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     4441 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     4501 gataatacca tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     4561 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     4621 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     4681 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     4741 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     4801 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     4861 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     4921 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     4981 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     5041 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     5101 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     5161 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     5221 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     5281 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     5341 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     5401 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     5461 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     5521 agggcaaagg aatagcccgg gcggggcgcg tctggaacaa tcaacctctg gattacaaaa
     5581 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5641 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     5701 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5761 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5821 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5881 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5941 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     6001 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     6061 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     6121 ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa
     6181 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     6241 agcacaagag gaggaggagg tgggtttttc cagtcacacc tcaggtacct ttaagaccaa
     6301 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
     6361 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     6421 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     6481 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     6541 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     6601 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     6661 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     6721 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     6781 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     6841 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6901 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6961 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7021 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgtttt
     7081 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7141 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7201 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7261 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7321 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7381 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7441 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7501 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7561 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7621 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7681 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7741 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7801 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7861 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7921 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     7981 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8041 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8101 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8161 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8221 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8281 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8341 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8401 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8461 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8521 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8581 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8641 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8701 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8761 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8821 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8881 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8941 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9001 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9061 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9121 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9181 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9241 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg
     9301 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9361 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     9421 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
     9481 ccactgccaa ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt
     9541 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa
     9601 attgtatttg ttaaatatgt actacaaact tagtagt
//
