LOCUS       Exported                6584 bp ds-DNA     circular SYN 07-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Marie-Eve Paquet
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 24, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
     misc_feature    156..640
                     /label=hSyn
     regulatory      674..679
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             680..727
                     /codon_start=1
                     /label=Influenza hemagglutinin
                     /translation="MKTIIALSYIFCLVLG"
     misc_feature    734..2500
                     /label=deLACCO1
     CDS             2561..2683
                     /codon_start=1
                     /label=GPI anchor (mouse NGR)
                     /translation="AGSGASGTGDAEGSGALPALACSLAPLGLALVLWTVLGPC"
     misc_feature    2705..3309
                     /label=WPRE
     polyA_signal    3329..3807
                     /label=hGH polyA
     misc_feature    3847..3987
                     /label=R-ITR
     rep_origin      4079..4385
                     /direction=RIGHT
                     /label=f1 Origin
     misc_feature    4904..5761
                     /label=AmpR
     rep_origin      5912..6579
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat cggacgaata
      661 acgcgtggat cccgccacca tgaagaccat catcgccctg tcctacatct tctgcctggt
      721 gctgggcaga tctatgttct ctcccttggc agtggcccag gctcgacgct ataggtggag
      781 gattcaaacc gcgtgggatg ccggaacagt aggctacagt ctgttccaaa agtttacgga
      841 gcgggtaaag gaactgacgg atggacagct tgaggtccag ccttttccag cgagtgcagt
      901 cgtaggcacg tttgatatgt tcgacgcggt caagactgga gttttggacg gaatgaatcc
      961 tttcacgctg tattgggcag ggaggatgcc ggttacagct ttcctctcca gctacgccct
     1021 cgggttggac cgacctgacc agtgggaaac atggttttac tccctgggtg gcctcgacag
     1081 cgcccgacga gcctttgccg agcaaggcct cttctacgtg ggaccagttc agcacgacct
     1141 caatacaatt cattcaaaga agccgatccg gagatttgag gacttcaaag gtgtcaagct
     1201 ccaagtgccg ggcggcatga ttgcggaagt ttttgcggcc gcaggagctt ccaccgtcct
     1261 cttgcctggg ggggaggtca accccgctct ggaaagaggg gtaatagatt ggagccacaa
     1321 cgtccatatc atggccgaca agcagaggaa cggcatcaag gcgaacttcg tgatccgcca
     1381 cagcaccgag gacggcggcg tgcagctcgc ctaccactac cagcagaaca cccccatcgg
     1441 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagacca aacttacgaa
     1501 agaccccaac gagaagcgcg atcacatggt cctgctggag tacgtgaccg ccgccgggat
     1561 cactcgcggc atggacgagc tgtacaaggg cggtaccaga gggagaatgg tgagcaaggg
     1621 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1681 ccacaagttc agcgtgtccg gcgagggtga gggcgatgcc acctccggca agctgtccct
     1741 gaagttcatc agcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1801 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     1861 caagtcggcc atgcccgaag gctacatcca ggagcgcacc atcttcttca aggacgacgg
     1921 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1981 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcataagc tggagtacag
     2041 ctttaacgtt ggtggtgcgg ctaattttgt tgggcctgca gtaaactata acttggggtt
     2101 ccatcaggag gcaaagtaca tcataatggg gcctccagaa acgcctgcca ttcatcaacc
     2161 tgtagacctc atggacttca ccataaacct taatcggtgg cgcagccttc caaagcccct
     2221 gcaagaaaga ttcatcgcgg cggttcacga gtactcttgg attcactacg caggcattca
     2281 gaaggctaat ttggaggcgt ggccgaaata tcggcaggca ggtgttgagg tcatccggct
     2341 gagcaacgag gatgttagaa aattcaggcg actcgctatt cccatttggt ttaagtgggc
     2401 taagatggat aactactcac gagaggcatt cgcgtctcaa ctggagtata tgaaaggtat
     2461 aggttacgtt acggacgagg aattgaaggg cttgggcctg ggctccacct ccggctccgg
     2521 caagcccggc tccggcgagg gctccaccaa gggcgaattc gcaggtagtg gtgcatctgg
     2581 aacaggagat gcagaaggat caggtgccct tcctgccctt gcatgtagtc tggctccact
     2641 gggactggca ttggttctgt ggacggtgtt gggcccctgt taggcggccg ctcgaatcaa
     2701 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     2761 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     2821 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     2881 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     2941 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     3001 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     3061 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     3121 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     3181 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     3241 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     3301 tcgataccga gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg
     3361 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta
     3421 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg
     3481 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa
     3541 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa
     3601 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc
     3661 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc
     3721 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg
     3781 tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc
     3841 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     3901 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     3961 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     4021 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     4081 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4141 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4201 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4261 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4321 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4381 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     4441 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4501 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     4561 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     4621 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     4681 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     4741 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     4801 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4861 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4921 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4981 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5041 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5101 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5161 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5221 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5281 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5341 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5401 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5461 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5521 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5581 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5641 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5701 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5761 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     5821 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5881 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5941 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6001 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6061 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     6121 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6181 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6241 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6301 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6361 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6421 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6481 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6541 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
