LOCUS       Exported                6791 bp ds-DNA     circular SYN 28-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Marie-Eve Paquet
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 24, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
     promoter        162..842
                     /label=gfaABC1D
                     /note="GFAP promoter"
     regulatory      852..857
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             858..905
                     /codon_start=1
                     /label=Influenza hemagglutinin
                     /translation="MKTIIALSYIFCLVLG"
     misc_feature    912..2678
                     /label=deLACCO1
     CDS             2739..2861
                     /codon_start=1
                     /label=GPI anchor (mouse NGR)
                     /translation="AGSGASGTGDAEGSGALPALACSLAPLGLALVLWTVLGPC"
     misc_feature    2908..3516
                     /label=WPRE
     polyA_signal    3536..4014
                     /label=hGH polyA
     misc_feature    4054..4194
                     /label=R-ITR
     rep_origin      4286..4592
                     /direction=RIGHT
                     /label=f1 Origin
     misc_feature    5111..5968
                     /label=AmpR
     rep_origin      6119..6786
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggatc taacatatcc tggtgtggag
      181 taggggacgc tgctctgaca gaggctcggg ggcctgagct ggctctgtga gctggggagg
      241 aggcagacag ccaggccttg tctgcaagca gacctggcag cattgggctg gccgcccccc
      301 agggcctcct cttcatgccc agtgaatgac tcaccttggc acagacacaa tgttcggggt
      361 gggcacagtg cctgcttccc gccgcacccc agcccccctc aaatgccttc cgagaagccc
      421 attgagcagg gggcttgcat tgcaccccag cctgacagcc tggcatcttg ggataaaagc
      481 agcacagccc cctaggggct gcccttgctg tgtggcgcca ccggcggtgg agaacaaggc
      541 tctattcagc ctgtgcccag gaaaggggat caggggatgc ccaggcatgg acagtgggtg
      601 gcaggggggg agaggagggc tgtctgcttc ccagaagtcc aaggacacaa atgggtgagg
      661 ggagagctct ccccatagct gggctgcggc ccaaccccac cccctcaggc tatgccaggg
      721 ggtgttgcca ggggcacccg ggcatcgcca gtctagccca ctccttcata aagccctcgc
      781 atcccaggag cgagcagagc cagagcaggt tggagaggag acgcatcacc tccgctgctc
      841 gcggggatcc cgccaccatg aagaccatca tcgccctgtc ctacatcttc tgcctggtgc
      901 tgggcagatc tatgttctct cccttggcag tggcccaggc tcgacgctat aggtggagga
      961 ttcaaaccgc gtgggatgcc ggaacagtag gctacagtct gttccaaaag tttacggagc
     1021 gggtaaagga actgacggat ggacagcttg aggtccagcc ttttccagcg agtgcagtcg
     1081 taggcacgtt tgatatgttc gacgcggtca agactggagt tttggacgga atgaatcctt
     1141 tcacgctgta ttgggcaggg aggatgccgg ttacagcttt cctctccagc tacgccctcg
     1201 ggttggaccg acctgaccag tgggaaacat ggttttactc cctgggtggc ctcgacagcg
     1261 cccgacgagc ctttgccgag caaggcctct tctacgtggg accagttcag cacgacctca
     1321 atacaattca ttcaaagaag ccgatccgga gatttgagga cttcaaaggt gtcaagctcc
     1381 aagtgccggg cggcatgatt gcggaagttt ttgcggccgc aggagcttcc accgtcctct
     1441 tgcctggggg ggaggtcaac cccgctctgg aaagaggggt aatagattgg agccacaacg
     1501 tccatatcat ggccgacaag cagaggaacg gcatcaaggc gaacttcgtg atccgccaca
     1561 gcaccgagga cggcggcgtg cagctcgcct accactacca gcagaacacc cccatcggcg
     1621 acggccccgt gctgctgccc gacaaccact acctgagcac ccagaccaaa cttacgaaag
     1681 accccaacga gaagcgcgat cacatggtcc tgctggagta cgtgaccgcc gccgggatca
     1741 ctcgcggcat ggacgagctg tacaagggcg gtaccagagg gagaatggtg agcaagggcg
     1801 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1861 acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctccggcaag ctgtccctga
     1921 agttcatcag caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     1981 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     2041 agtcggccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag gacgacggca
     2101 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     2161 tgaagggcat cgacttcaag gaggacggca acatcctggg gcataagctg gagtacagct
     2221 ttaacgttgg tggtgcggct aattttgttg ggcctgcagt aaactataac ttggggttcc
     2281 atcaggaggc aaagtacatc ataatggggc ctccagaaac gcctgccatt catcaacctg
     2341 tagacctcat ggacttcacc ataaacctta atcggtggcg cagccttcca aagcccctgc
     2401 aagaaagatt catcgcggcg gttcacgagt actcttggat tcactacgca ggcattcaga
     2461 aggctaattt ggaggcgtgg ccgaaatatc ggcaggcagg tgttgaggtc atccggctga
     2521 gcaacgagga tgttagaaaa ttcaggcgac tcgctattcc catttggttt aagtgggcta
     2581 agatggataa ctactcacga gaggcattcg cgtctcaact ggagtatatg aaaggtatag
     2641 gttacgttac ggacgaggaa ttgaagggct tgggcctggg ctccacctcc ggctccggca
     2701 agcccggctc cggcgagggc tccaccaagg gcgaattcgc aggtagtggt gcatctggaa
     2761 caggagatgc agaaggatca ggtgcccttc ctgcccttgc atgtagtctg gctccactgg
     2821 gactggcatt ggttctgtgg acggtgttgg gcccctgtta ggcggccgct cgacctcgag
     2881 gggcccggta cccgaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     2941 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     3001 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3061 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3121 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3181 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3241 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3301 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     3361 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     3421 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     3481 gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg
     3541 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     3601 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     3661 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     3721 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     3781 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
     3841 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     3901 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     3961 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt
     4021 ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
     4081 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     4141 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     4201 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     4261 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4321 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4381 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4441 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     4501 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4561 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt
     4621 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4681 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     4741 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     4801 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     4861 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     4921 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     4981 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5041 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5101 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5161 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5221 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5281 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5341 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5401 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5461 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5521 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5581 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5641 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5701 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5761 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5821 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5881 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5941 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6001 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6061 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6121 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6181 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6241 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6301 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6361 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6421 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6481 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6541 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6601 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6661 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6721 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6781 tgctcacatg t
//
