LOCUS       Exported                6021 bp ds-DNA     circular SYN 24-AUG-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Marie-Eve Paquet
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 24, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
     promoter        162..842
                     /label=gfaABC1D
                     /note="GFAP promoter"
     CDS             861..2135
                     /codon_start=1
                     /label=R-iLACCO1.2
                     /translation="MEQNIVQPLKTLMGDDPDYSFDILEARYAIEASTAWYAALRATPG
                     DKEKIQLCFEATRSEDPDIASQADVRFHLAIAEASHNIVLLQTMRGFFDVLQSSVKHSR
                     QRMYLDWVSERMFPEDGALKSEIKKGLRLKDGGHYAAEVKTTYKAKKPVQLPGAYVVDI
                     KLDIVSHNEDYTIVEQCERAEGRHSTGGVDELYKGGTGGSLVSKGEEDDMAIVKEFMRF
                     KVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYI
                     KHPADIPDYFKLSFPEGFRWERVMYFEDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPPD
                     GPVMQKKTMGWEARPPVYGQLTEQHQAVIDAIFAGDADGARKAMMAHLSFIHTTMKRFD
                     GDQARHARISRLPGEHNEHSREKKA"
     misc_feature    2138..2746
                     /label=WPRE
     polyA_signal    2766..3244
                     /label=hGH polyA
     misc_feature    3284..3424
                     /label=R-ITR
     rep_origin      3516..3822
                     /direction=RIGHT
                     /label=f1 Origin
     misc_feature    4341..5198
                     /label=AmpR
     rep_origin      5349..6016
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggatc taacatatcc tggtgtggag
      181 taggggacgc tgctctgaca gaggctcggg ggcctgagct ggctctgtga gctggggagg
      241 aggcagacag ccaggccttg tctgcaagca gacctggcag cattgggctg gccgcccccc
      301 agggcctcct cttcatgccc agtgaatgac tcaccttggc acagacacaa tgttcggggt
      361 gggcacagtg cctgcttccc gccgcacccc agcccccctc aaatgccttc cgagaagccc
      421 attgagcagg gggcttgcat tgcaccccag cctgacagcc tggcatcttg ggataaaagc
      481 agcacagccc cctaggggct gcccttgctg tgtggcgcca ccggcggtgg agaacaaggc
      541 tctattcagc ctgtgcccag gaaaggggat caggggatgc ccaggcatgg acagtgggtg
      601 gcaggggggg agaggagggc tgtctgcttc ccagaagtcc aaggacacaa atgggtgagg
      661 ggagagctct ccccatagct gggctgcggc ccaaccccac cccctcaggc tatgccaggg
      721 ggtgttgcca ggggcacccg ggcatcgcca gtctagccca ctccttcata aagccctcgc
      781 atcccaggag cgagcagagc cagagcaggt tggagaggag acgcatcacc tccgctgctc
      841 gcggggatcc tctagcgaga atggaacaaa acatagtcca gccgttgaag accctcatgg
      901 gagacgaccc tgattattca tttgatattc tggaggcgcg gtacgcgata gaagcgtcaa
      961 ctgcctggta cgctgcactg cgagctacac ccggggacaa agagaaaatt caactgtgct
     1021 tcgaggctac acggtccgaa gatcccgata tagcttccca agcagacgta aggttccacc
     1081 tggcgattgc tgaagcaagc cataacatag ttctgcttca gacaatgcga ggcttttttg
     1141 atgttttgca gtcttcagtc aagcacagtc ggcagcggat gtacttggat tgggtttccg
     1201 agcggatgtt ccccgaggac ggcgccctga agagcgagat caagaagggg ctgaggctga
     1261 aggacggcgg ccactacgcc gccgaggtca agaccactta caaggccaag aagcccgtgc
     1321 agctgcccgg cgcctacgtc gtcgacatca agttggacat cgtgtcccac aacgaggact
     1381 acactatcgt ggaacagtgc gaacgcgccg agggccgcca ctccaccggc ggcgtggacg
     1441 agctgtacaa gggaggtaca ggcgggagtc tggtgagcaa gggcgaggag gatgacatgg
     1501 ccatcgtcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgta aacggccacg
     1561 agttcgagat cgagggcgag ggcgagggcc gcccctacga ggcctttcag accgctaagc
     1621 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctatcc cctcagttca
     1681 tgtacggctc caaggcctac attaagcacc cagccgacat ccccgactac ttcaagctgt
     1741 ccttccccga gggcttcagg tgggagcgcg tgatgtactt cgaggacggc ggcattattc
     1801 acgttaacca ggactcctcc ctgcaggacg gcgtattcat ctacaaggtg aagctgcgcg
     1861 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcga
     1921 ggccgccggt ctacggtcaa ctcacagaac agcatcaggc agttatagac gccatctttg
     1981 ccggcgatgc ggacggtgct agaaaagcta tgatggctca tctgagcttc atccatacga
     2041 ctatgaaacg cttcgatgga gatcaggccc gacacgcacg catttccaga ctgcctggag
     2101 agcataatga gcactctcgg gaaaagaaag catgaaagct tatcgataat caacctctgg
     2161 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     2221 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     2281 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     2341 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     2401 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     2461 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     2521 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     2581 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     2641 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     2701 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga
     2761 gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
     2821 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
     2881 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg
     2941 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca
     3001 atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc
     3061 cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta
     3121 gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta
     3181 cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt
     3241 ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     3301 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     3361 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     3421 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3481 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3541 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3601 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3661 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     3721 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     3781 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     3841 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     3901 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     3961 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     4021 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     4081 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     4141 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4201 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4261 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4321 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4381 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4441 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4501 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4561 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4621 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4681 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4741 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4801 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4861 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4921 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4981 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5041 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5101 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5161 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5221 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5281 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5341 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5401 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5461 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     5521 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5581 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5641 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5701 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5761 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5821 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5881 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5941 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6001 gctggccttt tgctcacatg t
//
