LOCUS       pAAV-CASI-inteinC-aa713 7253 bp ds-DNA     circular     21-OCT-2021
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            addgene-plasmid-80932-sequence-155820.gbk Expresses Cas9C in
            mammalian cells; derived from pZac2.1 with CASI promoter..
FEATURES             Location/Qualifiers
     repeat_region   1..130
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     enhancer        206..509
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          377..396
                     /label="病毒滴度C-qPCR-F3"
                     /note="sequence: tcatatgccaagtacgcccc"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          380..399
                     /label="病毒滴度qPCR-F4"
                     /note="sequence: tatgccaagtacgcccccta"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          502..521
                     /label="病毒滴度C-qPCR-F1"
                     /note="sequence: ttaccatggtcgaggtgagc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     promoter        511..788
                     /label="chicken beta-actin promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          522..541
                     /label="病毒滴度C-qPCR-F2"
                     /note="sequence: cccacgttctgcttcactct"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(522..541)
                     /label="病毒滴度C-qPCR-R3"
                     /note="sequence: agagtgaagcagaacgtggg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1066..1085)
                     /label="病毒滴度C-qPCR-R4"
                     /note="sequence: tcacccaagtcccgtcctaa"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(1078..1097)
                     /label="病毒滴度C-qPCR-R1"
                     /note="sequence: agtgccctagagtcacccaa"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1180..1199)
                     /label="病毒滴度C-qPCR-R2"
                     /note="sequence: tagaggcatcatcggcgttc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     regulatory      1262..1271
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     regulatory      1266..1275
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1268..1292
                     /label="C-qPCR-titer-F"
                     /note="sequence: atggcggcggcgtgcccggaactgc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1268..1423
                     /label="Rma intein C"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             1268..1433
                     /label="Translation 1268-1433"
                     /translation="MAAACPELRQLAQSDVYWDPIVSIEPDGVEEVFDLTVPGPHNFVANDIIAHNSGQ"
     CDS             1268..3451
                     /label="Translation 1268-3451"
                     /translation="MAAACPELRQLAQSDVYWDPIVSIEPDGVEEVFDLTVPGPHNFVANDIIAHNSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVLDATLIHQSITGLYETRIDLSQLGGDSGGSKRTADGSEFEPKKKRKV"
     misc_feature    1417..1433
                     /label="插入SpG C前重后"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1424..1433
                     /label="Cas9-C-term"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1434..1435
                     /label="扩片段2-前"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1434..1453
                     /label="扩片段前"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1448..3451
                     /label="insert aa713 SpG C"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1604..1628)
                     /label="C-qPCR-titer-R"
                     /note="sequence: tcattctctcgcggctgttcttctg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1976..1995
                     /label="ABE7.10-C-QPCR-F-2"
                     /note="sequence: gacaatctgaccaaggccga"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          2469..2488
                     /label="ABE7.10-C-QPCR-F-1"
                     /note="sequence: tgatcgagacaaacggcgaa"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2687..2689
                     /label="D1335L"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2690..2692
                     /label="S1336W"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2700..2719)
                     /label="ABE7.10-C-QPCR-R-2"
                     /note="sequence: caccagcacagaataggcca"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2936..2938
                     /label="G1218K"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2939..2941
                     /label="E1219Q"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(2963..2982)
                     /label="ABE7.10-C-QPCR-R-1"
                     /note="sequence: acatatttggagggcagggc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          3026..3045
                     /label="ABE7.10-C-QPCR-F-3"
                     /note="sequence: tcccccgaggataatgagca"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3287..3289
                     /label="R1335Q"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3293..3295
                     /label="T1337R"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(3385..3404)
                     /label="ABE7.10-C-QPCR-R-3"
                     /note="sequence: tttttgagccgccagagtca"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3431..3451
                     /label="扩片段后"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             3431..3451
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3449..3451
                     /label="扩片段2-后"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3460..3486
                     /label="插入SpG C后重合"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_site      3466..3521
                     /label="-beta-globin poly(A) signal"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(3520..3539)
                     /label="rbglobpA-R"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3587..3605
                     /label="插入U6前"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(3626..3701)
                     /label="gRNA scaffold"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(3738..3978)
                     /label="U6 promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3989..4003
                     /label="插入U6后"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     repeat_region   4313..4442
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(4461..4477)
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(4461..4478)
                     /label="M13 Forward"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     complement(4470..4492)
                     /label="M13/pUC Forward"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      4619..5074
                     /label="f1 ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(4706..4725)
                     /label="F1ori-R"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     4916..4937
                     /label="F1ori-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        5101..5205
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             5206..6066
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(5424..5443)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      6237..6825
                     /label="ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     6726..6745
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     6979..6996
                     /label="L4440"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    7113..7134
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        7149..7179
                     /label="lac promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     protein_bind    7187..7203
                     /label="lac operator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     7192..7214
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     7211..7227
                     /label="M13 rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     7211..7227
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      241 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      301 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      361 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      421 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      481 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
      541 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
      601 gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg
      661 aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc
      721 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc
      781 ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc
      841 gccgcccgcc ccggctctga ctgaccgcgt tactaaaaca ggtaagtccg gcctccgcgc
      901 cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac
      961 gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc
     1021 tcataagact cggccttaga accccagtat cagcagaagg acattttagg acgggacttg
     1081 ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc
     1141 cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg atgcctctac
     1201 taaccatgtt catgttttct ttttttttct acaggtcctg ggtgacgaac aggctagcgc
     1261 cgccaccatg gcggcggcgt gcccggaact gcgtcagctg gcgcagagcg atgtgtattg
     1321 ggatccgatt gtgagcattg aaccggatgg cgtggaagaa gtgtttgatc tgaccgtgcc
     1381 gggcccgcat aactttgtgg cgaacgatat tattgcgcat aactctggcc agggcgatag
     1441 cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca
     1501 gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat
     1561 cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga
     1621 gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca
     1681 ccccgtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg
     1741 gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga
     1801 ccatatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag
     1861 aagcgacaag aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat
     1921 gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa
     1981 tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag
     2041 acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat
     2101 gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa
     2161 gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa
     2221 caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa
     2281 aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg
     2341 gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta
     2401 cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa
     2461 gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga
     2521 ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga
     2581 ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct
     2641 gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcctgt ggcccaccgt
     2701 ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag
     2761 tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat
     2821 cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc
     2881 taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccaagca
     2941 gctgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc
     3001 cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt
     3061 ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag
     3121 agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga
     3181 taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg
     3241 agcccctgcc gccttcaagt actttgacac caccatcgac cggaagcagt acagaagcac
     3301 caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg
     3361 gatcgacctg tctcagctgg gaggtgactc tggcggctca aaaagaaccg ccgacggcag
     3421 cgaattcgag cccaagaaga agaggaaagt cTAAACCGGt tggctaataa aggaaattta
     3481 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     3541 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     3601 atatgaggcc ctcacgcgta aaaaagcacc gactcggtgc cactttttca agttgataac
     3661 ggactagcct tattttaact tgctatttct agctctaaaa cctgagcagg tggaattcca
     3721 cctgcagtcg gtgtttcgtc ctttccacaa gatatataaa gccaagaaat cgaaatactt
     3781 tcaagttacg gtaagcatat gatagtccat tttaaaacat aattttaaaa ctgcaaacta
     3841 cccaagaaat tattactttc tacgtcacgt attttgtact aatatctttg tgtttacagt
     3901 caaattaatt ccaattatct ctctaacagc cttgtatcgt atatgcaaat atgaaggaat
     3961 catgggaaat aggccctcgg taccggcgat gctggctgcc atgaacaaag gttggctata
     4021 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     4081 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     4141 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     4201 agtcatagct gtccctcttc tcttatggag atcggatccg aattcccgat aaggatcttc
     4261 ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc
     4321 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     4381 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     4441 agccttaatt aacctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4501 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4561 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     4621 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4681 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4741 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4801 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4861 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4921 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4981 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5041 gcgaatttta acaaaatatt aacgtttata atttcaggtg gcatctttcg gggaaatgtg
     5101 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5161 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5221 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5281 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5341 gaactggatc tcaatagtgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5401 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5461 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5521 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5581 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5641 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5701 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agtaatggta
     5761 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5821 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5881 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5941 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6001 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6061 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6121 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6181 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6241 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6301 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6361 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     6421 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6481 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6541 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6601 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6661 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6721 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6781 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6841 gcctttttac ggttcctggc cttttgctgc ggttttgctc acatgttctt tcctgcgtta
     6901 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6961 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7021 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7081 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7141 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7201 caatttcaca caggaaacag ctatgaccat gattacgcca gatttaatta agg
//