LOCUS       pWZ699_YAV_with       14164 bp DNA     circular SYN 24-AUG-2023
DEFINITION  CEGS assembly vector LEU2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 24, 2023 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..474
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    651..850
                     /label=Lvec
     misc_feature    851..868
                     /label=I-SceI
     protein_bind    869..902
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     CDS             complement(956..1633)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     protein_bind    1669..1685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1693..1723)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1738..1759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    1871..1904
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    1905..1922
                     /label=I-SceI
     misc_feature    1923..2122
                     /label=Rvec
     CDS             2385..3479
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        3736..4219
                     /label=phPGK1_opt
                     /note="human phosphoglycerate kinase 1 promoter
                     Recoded for restriction sites: EagI, BsaI sites mutated 
                     with single bp based on alignment with mouse PGK promoter. 
                     BsmbI, SpeI sites mutated semi-randomly (just guessed and 
                     hoped)
                     "
     CDS             4225..5217
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995).
                     Recoded to remove several restriction sites."
                     /label=Delta-TKmod
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    5221..5342
                     /label=SV40pA
                     /note="SV40 polyadenylation signal"
     gene            complement(5354..6227)
                     /gene="Actb"
                     /gene_synonym="Act; Actx; beta-a; beta-actin;E430023M04Rik"
                     /label=Actb
                     /note="actin, beta; Derived by automated 
                     computationalanalysis using gene prediction method: 
                     BestRefSeq."
                     /db_xref="GeneID:11461"
                     /db_xref="MGI:MGI:87904"
     misc_feature    6229..6428
                     /label=eswap-in Rvec
     CDS             complement(6543..7358)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(7359..7461)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     misc_feature    7481..8761
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     misc_feature    7489..8266
                     /label=IS1 element
     rep_origin      9612..10226
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      10302..10521
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             10612..11367
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    11370..11620
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             11946..13121
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             13121..14092
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
ORIGIN
        1 ggcctgaata ttctctctgg gcccactgtt ccacttgtat cgtcggtctg ataatcagac
       61 tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc acggtcccac
      121 tcgtatcgtc ggtctgatta ttagtctggg accacggtcc cactcgtatc gtcggtctga
      181 taatcagact gggaccacgg tcccactcgt atcgtcggtc tgattattag tctgggacca
      241 tggtcccact cgtatcgtcg gtctgattat tagtctggga ccacggtccc actcgtatcg
      301 tcggtctgat tattagtctg gaaccacggt cccactcgta tcgtcggtct gattattagt
      361 ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacgatccca
      421 ctcgtgttgt cggtctgatt atcggtctgg gaccacggtc ccacttgtat tgtcgatcag
      481 actatcagcg tgagactacg attccatcaa tgcctgtcaa gggcaagtat tgacatgtcg
      541 tcgtaacctg tagaacggag taacctcggt gtgcggttgt atgcctgcgg tggattgctg
      601 ctgtgtcctg cttatccaca acattttgcg cacggttatg tggacgggcc taaggtagct
      661 accaatattt agtttctaag ccttgcgaca gacctcccac ttagattgcc acgcatagag
      721 ctagcgagtc agcgaaaagc atgacgcgct ttcaagcgtg gcgagtatgt gaaccaaggc
      781 ttcggacagg actatatact taggtttgat ctcgccccga gaactgtaaa cctcaacatt
      841 tatagattat tagggataac agggtaatat aacttcgtat aggatacttt atacgaagtt
      901 atcagtagag accgcctggc tctagtagcg atctacacta gcactatcag cgttattaag
      961 caccggtgga gtgacgacct tcagcacgtt cgtactgttc aacgatggtg tagtcttcgt
     1021 tgtgggaggt gatgtccagt ttgatgtcgg ttttgtaagc acccggcagc tgaaccggtt
     1081 ttttagccat gtaggtggtt ttaacttcag cgtcgtagtg accaccgtct ttcagtttca
     1141 gacgcatttt gatttcacct ttcagagcac cgtcttccgg gtacatacgt tcggtggaag
     1201 cttcccaacc catggttttt ttctgcataa ccggaccgtc ggacgggaag ttggtaccac
     1261 gcagtttaac tttgtagatg aactcaccgt cttgcaggga ggagtcctgg gtaacggtaa
     1321 caacaccacc gtcttcgaag ttcataacac gttcccattt gaaaccttcc gggaaggaca
     1381 gtttcaggta gtccgggatg tcagccgggt gtttaacgta agctttggaa ccgtactgga
     1441 actgcgggga caggatgtcc caagcgaacg gcagcggacc acctttggta actttcagtt
     1501 tagcggtctg ggtaccttcg tacggacgac cttcaccttc accttcgatt tcgaactcgt
     1561 gaccgttaac ggaaccttcc atacgaactt tgaaacgcat gaactctttg ataacgtctt
     1621 cggaggaagc catctagtat ttctcctctt tctctagtat gtgtgaaatt gttatccgct
     1681 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     1741 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     1801 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1861 gtctcatttt ataacttcgt atagcataca ttatacgaag ttattaggga taacagggta
     1921 attttagggt agcatcagga atctgaaccc tcagaaagtg gggatcccgg gtatagacct
     1981 ttatctgcgg ttcaagttag gcataaggct gcatgctacc ttgtcacacc tacactgctc
     2041 gaagtaaata tgggaagcgt gcgacctggc tccaggcgtt ccgcgccgcc acgtgttcgt
     2101 taactgttga ttggtggcac atagaggtcg cctgacgcat ataccttttt caactgaaaa
     2161 attgggagaa aaaggaaagg tgagagcgcc ggaaccggct tttcatatag aatagagaag
     2221 cgttcatgac taaatgcttg catcacaata cttgaagttg acaatattat ttaaggacct
     2281 attgtttttt ccaataggtg gttagcaatc gtcttacttt ctaacttttc ttacctttta
     2341 catttcagca atatatatat atatttcaag gatataccat tctaatgtct gcccctaaga
     2401 agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat cacagccgaa gccattaagg
     2461 ttcttaaagc tatttctgat gttcgttcca atgtcaagtt cgatttcgaa aatcatttaa
     2521 ttggtggtgc tgctatcgat gctacaggtg ttccacttcc agatgaggcg ctggaagcct
     2581 ccaagaaggc tgatgccgtt ttgttaggtg ctgtgggtgg tcctaaatgg ggtaccggta
     2641 gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg tacgccaact
     2701 taagaccatg taactttgca tccgactctc ttttagactt atctccaatc aagccacaat
     2761 ttgctaaagg tactgacttc gttgttgtca gagaattagt gggaggtatt tactttggta
     2821 agagaaagga agacgatggt gatggtgtcg cttgggatag tgaacaatac accgttccag
     2881 aagtgcaaag aatcacaaga atggccgctt tcatggccct acaacatgag ccaccattgc
     2941 ctatttggtc cttggataaa gctaatgttt tggcctcttc aagattatgg agaaaaactg
     3001 tggaggaaac catcaagaac gaattcccta cattgaaggt tcaacatcaa ttgattgatt
     3061 ctgccgccat gatcctagtt aagaacccaa cccacctaaa tggtattata atcaccagca
     3121 acatgtttgg tgatatcatc tccgatgaag cctccgttat cccaggttcc ttgggtttgt
     3181 tgccatctgc gtccttggcc tctttgccag acaagaacac cgcatttggt ttgtacgaac
     3241 catgccacgg ttctgctcca gatttgccaa agaataaggt caaccctatc gccactatct
     3301 tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt aaggccattg
     3361 aagatgcagt taaaaaggtt ttggatgcag gtatcagaac tggtgattta ggtggttcca
     3421 acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc cttgcttaaa
     3481 aagattctct ttttttatga tatttgtaca taaactttat aaatgaaatt cataatagaa
     3541 acgacacgaa attacaaaat ggaatatgtt catagggtag acgaaactat atacgcaatc
     3601 tacatacatt tatcaagaag gagaaaaagg aggatgtaaa ggaatacagg taagcaaatt
     3661 gatactaatg gctcaacgtg ataaggaaaa agaattgcac tttaacatta atattgacaa
     3721 ggaggagggc accgcggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca
     3781 gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggcctcg
     3841 cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc
     3901 cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg
     3961 ccggacgtga caaacggaag ccgcacgtct gactagtacc ctcgcagacg gacagcgcca
     4021 gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg
     4081 gcgcgccgag agcagcggct gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt
     4141 gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc
     4201 ggcagtcggc tccctcgttg gaggatgccc acgctactgc gggtttatat agacggtccc
     4261 cacgggatgg ggaaaaccac caccacgcaa ctgctggtgg ccctgggttc gcgcgacgat
     4321 atcgtctacg tacccgagcc gatgacttac tggcgggtgc tgggggcttc cgagacaatc
     4381 gcgaacatct acaccacaca acaccgcctc gaccagggtg agatatccgc cggggacgcg
     4441 gcggtggtaa tgacaagcgc ccagataaca atggggatgc cttatgccgt gaccgacgcc
     4501 gttctggctc ctcatatcgg gggggaggct gggagctcac atgccccgcc cccggccctc
     4561 accctcatct tcgaccgcca tcccatcgcc gccctcctgt gctaccccgc cgcgcgctac
     4621 cttatgggca gcatgacccc ccaggccgtg ctggcgttcg tggccctcat cccgccgacc
     4681 ttgcccggca ccaacatcgt gcttggggcc cttccggagg acagacacat cgaccgcctg
     4741 gccaaacgcc agcgccccgg cgagcggctg gacctggcta tgctggctgc gattcgccgc
     4801 gtttacgggc tacttgccaa tacggtgcgg tatctgcaat gcggcgggtc gtggcgggag
     4861 gactggggac agctttcggg gaccgccgtg ccgccccagg gtgccgagcc ccagagcaac
     4921 gcgggcccac gaccccatat cggggacacg ttatttaccc tgtttcgggc ccccgagttg
     4981 ctggccccca acggcgacct gtataacgtg tttgcctggg ccttggacgt cttggccaaa
     5041 cgcctccgtt ccatgcacgt ctttatcctg gattacgacc aatcgcccgc cggctgccgg
     5101 gacgccctgc tgcaacttac ctccgggatg gtccagaccc acgtcaccac ccccggctcc
     5161 ataccgacga tatgcgacct ggcgcgcacg tttgcccggg agatggggga ggctaactaa
     5221 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5281 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5341 tacctgcagg gatgccccag ggcatcggaa ccgctcgttg ccaatagtga tgacctggcc
     5401 gtcaggcagc tcatagctct tctccaggga ggaagaggat gcggcagtgg ccatctcctg
     5461 ctcgaagtct agagcaacat agcacagctt ctctttgatg tcacgcacga tttccctctc
     5521 agctgtggtg gtgaagctgt agccacgctc ggtcaggatc ttcatgaggt agtctgtcag
     5581 gtcccggcca gccaggtcca gacgcaggat ggcgtgaggg agagcatagc cctcgtagat
     5641 gggcacagtg tgggtgaccc cgtctccgga gtccatcaca atgcctgtgg tacgaccaga
     5701 ggcatacagg gacagcacag cctggatggc tacgtacatg gctggggtgt tgaaggtctc
     5761 aaacatgatc tgtagagaaa aataaataat gttgtcaagt tcagaaagcc acaagaaaca
     5821 ctcagggcag gtgaaactgt atggatagat ctgagacatg caaggagtgc aagaacacag
     5881 ctaagttcag tgtgctggga gtctcaggac aggggctcca cttagaccta ctgtgcatct
     5941 actcaataca cactccaagg ccacttatca ccagcctcat tagctttgtc acacgagcca
     6001 ttgttagtac ctaaacccac agcactgtag ggtttaaaca cctagtcaga aaggcaattg
     6061 agagaaaaaa agacctcgtc tgggaaagag cagaaactgc aaagatccaa gggagactca
     6121 gctcatagaa gggagtccta tgggagaacg gcagaagaaa gacaattgag aaagggcgtg
     6181 gctgagaagc tggccaaaga gaagggttac ccgggatact gacctggccc aactacgcag
     6241 ccgatcaaca acagaaacgc gacggtcgag atgaggaacg cacactggag cctttatcag
     6301 atggtgttga tttcaattac tccggagcgt gatgcggatc aaagtaaccg cgatggaagg
     6361 tgggggactc gaaatagatc cgttcttagc cctgcgaaac atctactgta caccacatgc
     6421 aacagccaca attctcatgt ttgacagctt atcatcgaat ttctgccatt catccgctta
     6481 ttatcactta ttcaggcgta gcaaccaggc gtttaagggc accaataact gccttaaaaa
     6541 aattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
     6601 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
     6661 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
     6721 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
     6781 actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc
     6841 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
     6901 tgcgcctgag ccagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
     6961 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
     7021 tattcttcta atacctggaa tgctgttttt ccggggatcg cagtggtgag taaccatgca
     7081 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
     7141 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
     7201 aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg
     7261 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
     7321 ggcctcgagc aagacgtttc ccgttgaata tggctcattt tagcttcctt agctcctgaa
     7381 aatctcgata actcaaaaaa tacgtccggt agtgatctta tttcattatg gtgaaagttg
     7441 gaacctctta cgtgccgatc aacgtccctg ggtccttttc atcacgtggg tgatgctgcc
     7501 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat
     7561 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
     7621 acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt tcacttacac
     7681 cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg gcgttggatg
     7741 ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc acttaaaaaa
     7801 ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg
     7861 gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg gctgttttac
     7921 gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg cactatggcg
     7981 acgctggggc gtcttatgag cctgctgtca ccctttgacg tggtgatatg gatgacggat
     8041 ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag caagcgatat
     8101 acgcagcgaa ttgagcggca taacctgaat ctgaggcagc acctggcacg gctgggacgg
     8161 aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg gcattatctg
     8221 aacataaaac actatcaata agttggagtc attacccatc acgtgctata aaaataatta
     8281 taatttaaat tttttaatat aaatatataa attaaaaata gaaagtaaaa aaagaaatta
     8341 aagaaaaaat agtttttgtt ttccgaagat gtaaaagact ctagggggat cgccaacaaa
     8401 tactaccttt tatcttgctc ttcctgctct caggtattaa tgccgaattg tttcatcttg
     8461 tctgtgtaga agaccacaca cgaaaatcct gtgattttac attttactta tcgttaatcg
     8521 aatgtatatc tatttaatct gcttttcttg tctaataaat atatatgtaa agtacgcttt
     8581 ttgttgaaat tttttaaacc tttgtttatt tttttttctt cattccgtaa ctcttctacc
     8641 ttctttattt actttctaaa atccaaatac aaaacataaa aataaataaa cacagagtaa
     8701 attcccaaat tattccatca ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat
     8761 cttttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt
     8821 attctgcgaa gtgatcttcc gtcacaggta tttattcgcg ataagctcat ggagcggcgt
     8881 aaccgtcgca caggaaggac agagaaagcg cggatctggg aagtgacgga cagaacggtc
     8941 aggacctgga ttggggaggc ggttgccgcc gctgctgctg acggtgtgac gttctctgtt
     9001 ccggtcacac cacatacgtt ccgccattcc tatgcgatgc acatgctgta tgccggtata
     9061 ccgctgaaag ttctgcaaag cctgatggga cataagtcca tcagttcaac ggaagtctac
     9121 acgaaggttt ttgcgctgga tgtggctgcc cggcaccggg tgcagtttgc gatgccggag
     9181 tctgatgcgg ttgcgatgct gaaacaatta tcctgagaat aaatgccttg gcctttatat
     9241 ggaaatgtgg aactgagtgg atatgctgtt tttgtctgtt aaacagagaa gctggctgtt
     9301 atccactgag aagcgagcga aacagtcggg aaaatctccc attatcgtag agatccgcat
     9361 tattaatctc aggagcctgt gtagcgttta taggaagtag tgttctgtca tgatgcctgc
     9421 aagcggtaac gaaaacgatt tgaatatgcc ttcaggaaca atagaaatct tcgtgcggtg
     9481 ttacgttgaa gtggagcgga ttatgtcagc aatggacaga acaacctaat gaacacagaa
     9541 ccatgatgtg gtctgtcctt ttacagccag tagtgctcgc cgcagtcgag cgacagggcg
     9601 aagccctcga gctggttgcc ctcgccgctg ggctggcggc tgtctatggc cctgcaaacg
     9661 cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccggccgccg gcgttgtgga
     9721 tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg
     9781 gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg
     9841 tggagctgga cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag
     9901 atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact
     9961 actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg
    10021 gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt
    10081 ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt
    10141 ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg
    10201 ggtgcccccc cttctcgaac cctcccggtc gagtgagcga ggaagcacca gggaacagca
    10261 cttatatatt ctgcttacac acgatgcctg aaaaaacttc ccttggggtt atccacttat
    10321 ccacggggat atttttataa ttattttttt tatagttttt agatcttctt ttttagagcg
    10381 ccttgtaggc ctttatccat gctggttcta gagaaggtgt tgtgacaaat tgccctttca
    10441 gtgtgacaaa tcaccctcaa atgacagtcc tgtctgtgac aaattgccct taaccctgtg
    10501 acaaattgcc ctcagaagaa gctgtttttt cacaaagtta tccctgctta ttgactcttt
    10561 tttatttagt gtgacaatct aaaaacttgt cacacttcac atggatctgt catggcggaa
    10621 acagcggtta tcaatcacaa gaaacgtaaa aatagcccgc gaatcgtcca gtcaaacgac
    10681 ctcactgagg cggcatatag tctctcccgg gatcaaaaac gtatgctgta tctgttcgtt
    10741 gaccagatca gaaaatctga tggcacccta caggaacatg acggtatctg cgagatccat
    10801 gttgctaaat atgctgaaat attcggattg acctctgcgg aagccagtaa ggatatacgg
    10861 caggcattga agagtttcgc ggggaaggaa gtggtttttt atcgccctga agaggatgcc
    10921 ggcgatgaaa aaggctatga atcttttcct tggtttatca aacgtgcgca cagtccatcc
    10981 agagggcttt acagtgtaca tatcaaccca tatctcattc ccttctttat cgggttacag
    11041 aaccggttta cgcagtttcg gcttagtgaa acaaaagaaa tcaccaatcc gtatgccatg
    11101 cgtttatacg aatccctgtg tcagtatcgt aagccggatg gctcaggcat tgtctctctg
    11161 aaaatcgact ggatcataga gcgttaccag ctgcctcaaa gttaccagcg tatgcctgac
    11221 ttccgccgcc gcttcctgca ggtctgtgtt aatgagatca acagcagaac tccaatgcgc
    11281 ctctcataca ttgagaaaaa gaaaggccgc cagacgactc atatcgtatt ttccttccgc
    11341 gatatcactt ccatgacgac aggatagtct gagggttatc tgtcacagat ttgagggtgg
    11401 ttcgtcacat ttgttctgac ctactgaggg taatttgtca cagttttgct gtttccttca
    11461 gcctgcatgg attttctcat actttttgaa ctgtaatttt taaggaagcc aaatttgagg
    11521 gcagtttgtc acagttgatt tccttctctt tcccttcgtc atgtgacctg atatcggggg
    11581 ttagttcgtc atcattgatg agggttgatt atcacagttt attactctga attggctatc
    11641 cgcgtgtgta cctctacctg gagtttttcc cacggtggat atttcttctt gcgctgagcg
    11701 taagagctat ctgacagaac agttcttctt tgcttcctcg ccagttcgct cgctatgctc
    11761 ggttacacgg ctgcggcgag cgctagtgat aataagtgac tgaggtatgt gctcttctta
    11821 tctccttttg tagtgttgct cttattttaa acaactttgc ggttttttga tgactttgcg
    11881 attttgttgt tgctttgcag taaattgcaa gatttaataa aaaaacgcaa agcaatgatt
    11941 aaaggatgtt cagaatgaaa ctcatggaaa cacttaacca gtgcataaac gctggtcatg
    12001 aaatgacgaa ggctatcgcc attgcacagt ttaatgatga cagcccggaa gcgaggaaaa
    12061 taacccggcg ctggagaata ggtgaagcag cggatttagt tggggtttct tctcaggcta
    12121 tcagagatgc cgagaaagca gggcgactac cgcacccgga tatggaaatt cgaggacggg
    12181 ttgagcaacg tgttggttat acaattgaac aaattaatca tatgcgtgat gtgtttggta
    12241 cgcgattgcg acgtgctgaa gacgtatttc caccggtgat cggggttgct gcccataaag
    12301 gtggcgttta caaaacctca gtttctgttc atcttgctca ggatctggct ctgaaggggc
    12361 tacgtgtttt gctcgtggaa ggtaacgacc cccagggaac agcctcaatg tatcacggat
    12421 gggtaccaga tcttcatatt catgcagaag acactctcct gcctttctat cttggggaaa
    12481 aggacgatgt cacttatgca ataaagccca cttgctggcc ggggcttgac attattcctt
    12541 cctgtctggc tctgcaccgt attgaaactg agttaatggg caaatttgat gaaggtaaac
    12601 tgcccaccga tccacacctg atgctccgac tggccattga aactgttgct catgactatg
    12661 atgtcatagt tattgacagc gcgcctaacc tgggtatcgg cacgattaat gtcgtatgtg
    12721 ctgctgatgt gctgattgtt cccacgcctg ctgagttgtt tgactacacc tccgcactgc
    12781 agtttttcga tatgcttcgt gatctgctca agaacgttga tcttaaaggg ttcgagcctg
    12841 atgtacgtat tttgcttacc aaatacagca atagcaatgg ctctcagtcc ccgtggatgg
    12901 aggagcaaat tcgggatgcc tggggaagca tggttctaaa aaatgttgta cgtgaaacgg
    12961 atgaagttgg taaaggtcag atccggatga gaactgtttt tgaacaggcc attgatcaac
    13021 gctcttcaac tggtgcctgg agaaatgctc tttctatttg ggaacctgtc tgcaatgaaa
    13081 ttttcgatcg tctgattaaa ccacgctggg agattagata atgaagcgtg cgcctgttat
    13141 tccaaaacat acgctcaata ctcaaccggt tgaagatact tcgttatcga caccagctgc
    13201 cccgatggtg gattcgttaa ttgcgcgcgt aggagtaatg gctcgcggta atgccattac
    13261 tttgcctgta tgtggtcggg atgtgaagtt tactcttgaa gtgctccggg gtgatagtgt
    13321 tgagaagacc tctcgggtat ggtcaggtaa tgaacgtgac caggagctgc ttactgagga
    13381 cgcactggat gatctcatcc cttcttttct actgactggt caacagacac cggcgttcgg
    13441 tcgaagagta tctggtgtca tagaaattgc cgatgggagt cgccgtcgta aagctgctgc
    13501 acttaccgaa agtgattatc gtgttctggt tggcgagctg gatgatgagc agatggctgc
    13561 attatccaga ttgggtaacg attatcgccc aacaagtgct tatgaacgtg gtcagcgtta
    13621 tgcaagccga ttgcagaatg aatttgctgg aaatatttct gcgctggctg atgcggaaaa
    13681 tatttcacgt aagattatta cccgctgtat caacaccgcc aaattgccta aatcagttgt
    13741 tgctcttttt tctcaccccg gtgaactatc tgcccggtca ggtgatgcac ttcaaaaagc
    13801 ctttacagat aaagaggaat tacttaagca gcaggcatct aaccttcatg agcagaaaaa
    13861 agctggggtg atatttgaag ctgaagaagt tatcactctt ttaacttctg tgcttaaaac
    13921 gtcatctgca tcaagaacta gtttaagctc acgacatcag tttgctcctg gagcgacagt
    13981 attgtataag ggcgataaaa tggtgcttaa cctggacagg tcccgtgttc caactgagtg
    14041 tatagagaaa attgaggcca ttcttaagga acttgaaaag ccagcaccct gatgcgacct
    14101 cgttttagtc tacgtttatc tgtctttact taatgtcctt tgttacaggc cagaaagcat
    14161 aact
//
