LOCUS       CN3568-pAAV-3xcore1_eHGT_387m_minBG-SYFP2-WPRE3-BGHpA        4772 bp    DNA     circular     23-AUG-2023
DEFINITION  .
ACCESSION   urn.local...o-dnus9oa
VERSION     urn.local...o-dnus9oa
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Author-START## Name :: Jonathan Ting ##Author-END##              
            ##Comment-START## Comment ::                                       
            rAAV-hPTenh(FAM84b)-minBG-SYFP2-WPRE3-BGHpA            clones 2,3,4
            all good            clone 1 low yield ##Comment-END##              
            ##Imported_From-START## Path     :: C:\VNTI Database Filename ::   
            _exdata.ma4 ##Imported_From-END##                                  
                                                                               
            ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /vntifkey=21
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     Enhancer        145..695
                     /created_by="ximena.opitzaraya"
                     /locus_tag="3xcore1_eHGT_387m"
                     /ApEinfo_label="3xcore1_eHGT_387m"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="3xcore1_eHGT_387m"
     misc_feature    697..749
                     /locus_tag="Beta Globin minimal promoter"
                     /ApEinfo_label="Beta Globin minimal promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta Globin minimal promoter"
     misc_feature    790..1509
                     /locus_tag="SYFP2"
                     /ApEinfo_label="SYFP2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="SYFP2"
     misc_feature    1544..1789
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="WPRE3"
     misc_feature    1803..2006
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="BGH-pA"
     misc_feature    2035..2175
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="R-ITR"
     rep_origin      2267..2573
                     /locus_tag="f1 Origin"
                     /ApEinfo_label="f1 Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="f1 Origin"
     misc_feature    3092..3949
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="AmpR"
     rep_origin      4100..4767
                     /locus_tag="pUC ori"
                     /ApEinfo_label="pUC ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtataagg agggtctttg tttctcttcc ctgggaaccg
      181 agcagcccct ttctctgagg gtagagttgg ggcatggctc aacagaacga cagtcatcct
      241 ggctagtaag actcagaggc tggccttcaa aggcttgagc tcctgggtga ctgagcagtg
      301 gagaaacaga atcctgccct tgaattgcat aaggagggtc tttgtttctc ttccctggga
      361 accgagcagc ccctttctct gagggtagag ttggggcatg gctcaacaga acgacagtca
      421 tcctggctag taagactcag aggctggcct tcaaaggctt gagctcctgg gtgactgagc
      481 agtggagaaa cagaatcctg cccttgaatt gcataaggag ggtctttgtt tctcttccct
      541 gggaaccgag cagccccttt ctctgagggt agagttgggg catggctcaa cagaacgaca
      601 gtcatcctgg ctagtaagac tcagaggctg gccttcaaag gcttgagctc ctgggtgact
      661 gagcagtgga gaaacagaat cctgcccttg aattgcgggc tgggcataaa agtcagggca
      721 gagccatcta ttgcttacat ttgcttctgg gatccagatc tttcgaagct agcgctaccg
      781 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      841 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      901 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc
      961 tggcccaccc tcgtgaccac cctgggctac ggcgtgcagt gcttcgcccg ctaccccgac
     1021 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1081 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1141 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1201 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag
     1261 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     1321 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1381 gacaaccact acctgagcta ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     1441 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1501 tacaagtaag tcgacggcgc gccgcggccg cgaattcgat atcataatca acctctggat
     1561 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     1621 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     1681 tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc cgcctgcctt
     1741 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggc tcgagagatc
     1801 ttcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     1861 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     1921 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     1981 ggattgggaa gacaatagca ggcatgagat ctcacgtgcg gaccgagcgg ccgcaggaac
     2041 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     2101 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     2161 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     2221 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     2281 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     2341 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     2401 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     2461 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     2521 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     2581 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     2641 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     2701 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     2761 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     2821 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     2881 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     2941 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3001 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3061 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3121 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3181 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3241 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3301 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3361 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3421 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3481 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3541 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3601 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3661 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3721 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3781 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3841 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     3901 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     3961 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4021 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4081 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4141 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4201 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4261 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4321 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4381 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4441 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4501 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4561 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4621 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4681 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4741 cctggccttt tgctggcctt ttgctcacat gt
//
