LOCUS       CN2556-pAAV-3xcore_eHGT_410m-minBglobin-SYFP2-WPRE3-BGHpA        4829 bp    DNA     circular     23-AUG-2023
DEFINITION  .
ACCESSION   urn.local...f-bsfghod
VERSION     urn.local...f-bsfghod
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Comment-START## Comment :: rAAV-ESYN-SYFP2-WPRE3-BGHpA clone #8-1
            correct , 8-2 backup ##Comment-END## ##Imported_From-START## Path  
            :: C:\VNTI Database Filename :: _exdata.ma4 ##Imported_From-END##  
            ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /vntifkey=21
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     Enhancer        146..753
                     /created_by="ximena.opitzaraya"
                     /locus_tag="3xcore_eHGT_410m"
                     /ApEinfo_label="3xcore_eHGT_410m"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="3xcore_eHGT_410m"
     misc_feature    760..812
                     /locus_tag="Beta Globin minimal promoter"
                     /ApEinfo_label="Beta Globin minimal promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta Globin minimal promoter"
     misc_feature    853..1572
                     /locus_tag="SYFP2"
                     /ApEinfo_label="SYFP2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="SYFP2"
     misc_feature    1607..1852
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="WPRE3"
     misc_feature    1866..2069
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="BGH-pA"
     misc_feature    2092..2232
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="R-ITR"
     rep_origin      2324..2630
                     /locus_tag="f1 Origin"
                     /ApEinfo_label="f1 Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="f1 Origin"
     misc_feature    3149..4006
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="AmpR"
     rep_origin      4157..4824
                     /locus_tag="pUC ori"
                     /ApEinfo_label="pUC ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtacactc ttccctttat tctttcttcc aggcctttta
      181 gtgtttgctt aatcagaata ttttaatctc taatgtggtg actagatgta atttatcaca
      241 ttaagcctct cctattttct tctgcttaca tgactaacgt tattgtgttt ttattgcgct
      301 tatacaaaca agcctttcct ttgttccatt gtttcagcaa taggtcaaca ctcttccctt
      361 tattctttct tccaggcctt ttagtgtttg cttaatcaga atattttaat ctctaatgtg
      421 gtgactagat gtaatttatc acattaagcc tctcctattt tcttctgctt acatgactaa
      481 cgttattgtg tttttattgc gcttatacaa acaagccttt cctttgttcc attgtttcag
      541 caataggtca acactcttcc ctttattctt tcttccaggc cttttagtgt ttgcttaatc
      601 agaatatttt aatctctaat gtggtgacta gatgtaattt atcacattaa gcctctccta
      661 ttttcttctg cttacatgac taacgttatt gtgtttttat tgcgcttata caaacaagcc
      721 tttcctttgt tccattgttt cagcaatagg tcagagctcg ggctgggcat aaaagtcagg
      781 gcagagccat ctattgctta catttgcttc tgggatccag atctttcgaa gctagcgcta
      841 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      901 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      961 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
     1021 ccctggccca ccctcgtgac caccctgggc tacggcgtgc agtgcttcgc ccgctacccc
     1081 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1141 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1201 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1261 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     1321 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     1381 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1441 cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc
     1501 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1561 ctgtacaagt aagtcgacgg cgcgccgcgg ccgcgaattc gatatcataa tcaacctctg
     1621 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     1681 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     1741 ttctcctcct tgtataaatc ctggttagtt cttgccacgg cggaactcat cgccgcctgc
     1801 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggctcgagag
     1861 atcttcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     1921 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     1981 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     2041 ggaggattgg gaagacaata gcaggcatgc acgtgcggac cgagcggccg caggaacccc
     2101 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     2161 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     2221 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     2281 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     2341 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     2401 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2461 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2521 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     2581 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     2641 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     2701 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     2761 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     2821 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     2881 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     2941 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     3001 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3061 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3121 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3181 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3241 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3301 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3361 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3421 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3481 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3541 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3601 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3661 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3721 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3781 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3841 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     3901 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3961 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4021 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4081 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4141 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4201 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4261 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4321 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4381 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4441 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4501 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4561 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4621 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4681 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4741 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4801 ggccttttgc tggccttttg ctcacatgt
//
