LOCUS       CN2677-pAAV-eHGT_401h-minBglobin-SYFP2-WPRE3-BGHpA        4530 bp    DNA     circular     09-AUG-2023
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...2yu-c1qk8k6
VERSION     urn.local...2yu-c1qk8k6
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /vntifkey=21
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     Enhancer        145..448
                     /Color="#ffff55"
                     /created_by="refugio.martinez"
                     /locus_tag="eHGT_401h"
                     /ApEinfo_label="eHGT_401h"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="eHGT_401h"
     misc_feature    455..507
                     /vntifkey=21
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta\Globin\minimal\promoter"
     misc_feature    548..1267
                     /vntifkey=21
                     /locus_tag="SYFP2"
                     /ApEinfo_label="SYFP2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="SYFP2"
     misc_feature    1302..1547
                     /vntifkey=21
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="WPRE3"
     misc_feature    1561..1764
                     /vntifkey=21
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BGH-pA"
     misc_feature    1793..1933
                     /vntifkey=21
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="R-ITR"
     rep_origin      2025..2331
                     /vntifkey=33
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="f1\Origin"
     misc_feature    2850..3707
                     /vntifkey=21
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR"
     rep_origin      3858..4525
                     /vntifkey=33
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcaccac tctgtgctta gaaccaagag ctaggagagc
      181 aagggagcct gggactggca cttttcagct agggggagct ggtggtggca gggcagagag
      241 gcccattgcg cagctggtaa caggctgtgc ctggccaggc ctcccagctg cccctctgtc
      301 tcaggccctt attgcacaca acatccagcc cagcccccta cgcagctgcc ttcctcatga
      361 cacagccctg tgttgtcctg ttggccactg accactgtgc ctggggaagg gccaggcctt
      421 cctgggagga ggaactcgag agcctgtgga gctcgggctg ggcataaaag tcagggcaga
      481 gccatctatt gcttacattt gcttctggga tccagatctt tcgaagctag cgctaccggt
      541 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      601 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      661 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
      721 gcccaccctc gtgaccaccc tgggctacgg cgtgcagtgc ttcgcccgct accccgacca
      781 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      841 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      901 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      961 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca
     1021 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca
     1081 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1141 caaccactac ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     1201 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1261 caagtaagtc gacggcgcgc cgcggccgcg aattcgatat cataatcaac ctctggatta
     1321 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     1381 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     1441 ctccttgtat aaatcctggt tagttcttgc cacggcggaa ctcatcgccg cctgccttgc
     1501 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggctc gagagatctt
     1561 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     1621 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     1681 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     1741 attgggaaga caatagcagg catgagatct cacgtgcgga ccgagcggcc gcaggaaccc
     1801 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     1861 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     1921 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     1981 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     2041 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     2101 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     2161 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     2221 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     2281 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     2341 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     2401 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     2461 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     2521 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     2581 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     2641 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     2701 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2761 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2821 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2881 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2941 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3001 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3061 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     3121 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3181 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3241 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3301 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3361 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3421 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3481 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3541 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3601 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3661 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3721 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3781 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3841 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3901 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3961 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4021 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4081 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4141 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4201 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4261 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4321 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4381 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4441 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4501 tggccttttg ctggcctttt gctcacatgt
//
