LOCUS       CN2499-pAAV-eHGT_403h-minBglobin-SYFP2-WPRE3-BGHpA        4620 bp    DNA     circular     09-AUG-2023
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...f51-bf64jy5
VERSION     urn.local...f51-bf64jy5
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /vntifkey=21
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     Enhancer        145..538
                     /created_by="refugio.martinez"
                     /locus_tag="eHGT_403h"
                     /ApEinfo_label="eHGT_403h"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="eHGT_403h"
     misc_feature    545..597
                     /vntifkey=21
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta\Globin\minimal\promoter"
     misc_feature    638..1357
                     /vntifkey=21
                     /locus_tag="SYFP2"
                     /ApEinfo_label="SYFP2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="SYFP2"
     misc_feature    1392..1637
                     /vntifkey=21
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="WPRE3"
     misc_feature    1651..1854
                     /vntifkey=21
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BGH-pA"
     misc_feature    1883..2023
                     /vntifkey=21
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="R-ITR"
     rep_origin      2115..2421
                     /vntifkey=33
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="f1\Origin"
     misc_feature    2940..3797
                     /vntifkey=21
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR"
     rep_origin      3948..4615
                     /vntifkey=33
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgcgagt ccaggggctc taccccctct tctgccctcc
      181 ttcccctccc tccagggtgg ttcagtactc gctgggatgg gggactctaa cccaagtcct
      241 ccccgcaccc atggttctag tcacatctgt caaagggatc caggcaagaa caaaggccta
      301 gtgtgcctcg gacaaaggag aaaggtcccg ccagccctca cccctccctg ggccctgcgt
      361 ctgccccctc gggaccgcca ccatggaaac cccctcccac actcactaat agcccccgtg
      421 ggtgcccagg acctgagcca tcgccagggc aacagggctg gttccgacac aggcgtgtgt
      481 cccgcagtgt ccattcccaa gtgccctccc ttcgatgggg aacacggcag gactccatga
      541 gctcgggctg ggcataaaag tcagggcaga gccatctatt gcttacattt gcttctggga
      601 tccagatctt tcgaagctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
      661 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      721 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat
      781 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg
      841 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      901 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      961 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1021 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1081 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
     1141 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
     1201 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct
     1261 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1321 cgggatcact ctcggcatgg acgagctgta caagtaagtc gacggcgcgc cgcggccgcg
     1381 aattcgatat cataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     1441 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     1501 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tagttcttgc
     1561 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     1621 cactgacaat tccgtggctc gagagatctt cgactgtgcc ttctagttgc cagccatctg
     1681 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     1741 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     1801 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgagatct
     1861 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
     1921 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     1981 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
     2041 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     2101 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     2161 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     2221 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     2281 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     2341 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     2401 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
     2461 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2521 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     2581 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     2641 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     2701 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     2761 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     2821 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2881 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     2941 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3001 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3061 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3121 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3181 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3241 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3301 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3361 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3421 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3481 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3541 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     3601 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     3661 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     3721 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     3781 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     3841 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3901 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3961 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4021 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4081 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4141 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4201 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4261 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4321 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4381 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4441 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4501 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4561 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
