LOCUS       CN3998-pAAV-eHGT_390m-minBglobin-iCre(R297T)-BGHpA        4980 bp    DNA     circular     24-AUG-2023
DEFINITION  .
ACCESSION   urn.local...pt-el6rg94
VERSION     urn.local...pt-el6rg94
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Author-START## Name :: Jonathan Ting ##Author-END##              
            ##Imported_From-START## Path     ::                                
            \\allen\programs\celltypes\workgroups\0285\CN-VectorNTI Filename ::
            CN3084 (JT).gb ##Imported_From-END##                               
            ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /vntifkey=21
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     Enhancer        145..840
                     /created_by="refugio.martinez"
                     /locus_tag="eHGT_390m"
                     /ApEinfo_label="eHGT_390m"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="eHGT_390m"
     misc_feature    847..899
                     /vntifkey=21
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta\Globin\minimal\promoter"
     CDS             928..1983
                     /vntifkey=21
                     /locus_tag="iCre(297T)"
                     /ApEinfo_label="iCre(297T)"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /modified_by="adminkellyyun"
                     /label="iCre(297T)"
     misc_feature    928..954
                     /vntifkey=21
                     /locus_tag="nls"
                     /ApEinfo_label="nls"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="nls"
     misc_feature    1840..1842
                     /vntifkey=21
                     /locus_tag="R297T"
                     /ApEinfo_label="R297T"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="R297T"
     misc_feature    1903..1905
                     /vntifkey=21
                     /locus_tag="V318"
                     /ApEinfo_label="V318"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="V318"
     polyA_site      2017..2220
                     /vntifkey=21
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /modified_by="adminkellyyun"
                     /label="BGH-pA"
     misc_feature    2243..2383
                     /vntifkey=21
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="R-ITR"
     rep_origin      2475..2781
                     /vntifkey=33
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="f1\Origin"
     misc_feature    3300..4157
                     /vntifkey=21
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="AmpR"
     rep_origin      4308..4975
                     /vntifkey=33
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttccaga ccttgaacat aaaaggcatt ttatacccgc
      181 aattgttcat ttaatgagca attgcggagt gcaggccggt taagggattg agctatatgc
      241 actattattg caagaagtat tccgaaatac cagaaatagg acgtaagctc tgatcaggga
      301 gactgcgagc acaattacct tcttttcaaa tccttctgtg acactgcggg aggaaaaagg
      361 actttgaaac ttgaaaggaa agagcttgct ttcaacctca aaagctagga ggaaagggct
      421 ctgaaatttg ctcagaattc ccaattcacc attagcctgt ttcttccttt agcctcaagg
      481 cattctccgc tttttgaaaa gatgttaaga aattcagtca caatagagag cctagttttg
      541 aacatgtttc actcggtcca ttgaggtctg ggctccagcc tttgtgtggg gtgaattgag
      601 ctgagcggct agctggttgg agagaggtga atgagaagtc gctgtgcagt tgcaaattct
      661 ggcaaagaaa aaaaaaaagc tcaccccttc ctttattttg taatatgcat tcctgtacaa
      721 tcctgccagt ggcaatctgt ggagttcagt gtgtccctaa gtcaatatgg agtacttggt
      781 ttatagcaac tcttgttaag tttgtcttgt aattgaagct gctgttgacc ttgcttgggg
      841 gagctcgggc tgggcataaa agtcagggca gagccatcta ttgcttacat ttgcttctgg
      901 gatccagatc tttccaagct agccaccatg gtgcccaaga agaagaggaa agtctccaac
      961 ctgctgactg tgcaccaaaa cctgcctgcc ctccctgtgg atgccacctc tgatgaagtc
     1021 aggaagaacc tgatggacat gttcagggac aggcaggcct tctctgaaca cacctggaag
     1081 atgctcctgt ctgtgtgcag atcctgggct gcctggtgca agctgaacaa caggaaatgg
     1141 ttccctgctg aacctgagga tgtgagggac tacctcctgt acctgcaagc cagaggcctg
     1201 gctgtgaaga ccatccaaca gcacctgggc cagctcaaca tgctgcacag gagatctggc
     1261 ctgcctcgcc cttctgactc caatgctgtg tccctggtga tgaggagaat cagaaaggag
     1321 aatgtggatg ctggggagag agccaagcag gccctggcct ttgaacgcac tgactttgac
     1381 caagtcagat ccctgatgga gaactctgac agatgccagg acatcaggaa cctggccttc
     1441 ctgggcattg cctacaacac cctgctgcgc attgccgaaa ttgccagaat cagagtgaag
     1501 gacatctccc gcaccgatgg tgggagaatg ctgatccaca ttggcaggac caagaccctg
     1561 gtgtccacag ctggtgtgga gaaggccctg tccctggggg ttaccaagct ggtggagaga
     1621 tggatctctg tgtctggtgt ggctgatgac cccaacaact acctgttctg ccgggtcaga
     1681 aagaatggtg tggctgcccc ttctgccacc tcccaactgt ccacccgggc cctggaaggg
     1741 atctttgagg ccacccaccg cctgatctat ggtgccaagg atgactctgg gcagagatac
     1801 ctggcctggt ctggccactc tgccagagtg ggtgctgcca ccgacatggc cagggctggt
     1861 gtgtccatcc ctgaaatcat gcaggctggt ggctggacca atgtgaacat tgtgatgaac
     1921 tacatcagaa acctggactc tgagactggg gccatggtga ggctgctcga agatggggac
     1981 tgaggcgcgc cgaattcaag cttctcgaga gatcttcgac tgtgccttct agttgccagc
     2041 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     2101 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     2161 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     2221 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
     2281 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     2341 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
     2401 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     2461 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     2521 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     2581 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     2641 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     2701 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     2761 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
     2821 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2881 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     2941 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     3001 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     3061 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     3121 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     3181 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3241 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3301 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3361 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3421 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3481 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3541 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3601 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3661 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3721 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3781 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3841 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3901 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     3961 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4021 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4081 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4141 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4201 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4261 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4321 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4381 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4441 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4501 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4561 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4621 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4681 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4741 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4801 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4861 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4921 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
