LOCUS       CN4534-pAAV-3xcore2_eHGT_390m-minBglobin-iAChSnFR-Venus-WPRE3-BGHpA        6071 bp    DNA     circular     23-AUG-2023
DEFINITION  .
ACCESSION   urn.local...10-bsfuej6
VERSION     urn.local...10-bsfuej6
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Comment-START## Comment :: rAAV-ESYN-SYFP2-WPRE3-BGHpA clone #8-1
            correct , 8-2            backup ##Comment-END##                    
            ##Imported_From-START## Path     :: C:\VNTI Database Filename ::   
            _exdata.ma4 ##Imported_From-END##                                  
                                                                               
            ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /vntifkey=21
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     Enhancer        145..894
                     /created_by="ximena.opitzaraya"
                     /locus_tag="3xcore2_eHGT_390m"
                     /ApEinfo_label="3xcore2_eHGT_390m"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="3xcore2_eHGT_390m"
     misc_feature    901..953
                     /locus_tag="Beta Globin minimal promoter"
                     /ApEinfo_label="Beta Globin minimal promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta Globin minimal promoter"
     misc_feature    994..2820
                     /locus_tag="iAChSnFR-Venus"
                     /ApEinfo_label="iAChSnFR-Venus"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="iAChSnFR-Venus"
     sig_peptide     994..1056
                     /product="leader sequence from mouse immunoglobulin kappa
                     light chain"
                     /locus_tag="Ig-kappa leader"
                     /ApEinfo_label="Ig-kappa leader"
                     /ApEinfo_fwdcolor="#00D95A"
                     /ApEinfo_revcolor="#00D95A"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="Ig-kappa leader"
     unsure          1294..1569
                     /Color="#ddff55"
                     /created_by="refugio.martinez"
                     /locus_tag="SFGFP 147-238"
                     /ApEinfo_label="SFGFP 147-238"
                     /ApEinfo_fwdcolor="#13AAFE"
                     /ApEinfo_revcolor="#13AAFE"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="SFGFP 147-238"
     unsure          1570..1587
                     /Color="#118800"
                     /created_by="refugio.martinez"
                     /locus_tag="Circular permutation linker"
                     /ApEinfo_label="Circular permutation linker"
                     /ApEinfo_fwdcolor="#3A93FE"
                     /ApEinfo_revcolor="#3A93FE"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="Circular permutation linker"
     unsure          1588..2025
                     /Color="#bbff33"
                     /created_by="refugio.martinez"
                     /locus_tag="SFGFP 1-146"
                     /ApEinfo_label="SFGFP 1-146"
                     /ApEinfo_fwdcolor="#2D64FE"
                     /ApEinfo_revcolor="#2D64FE"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="SFGFP 1-146"
     CDS             2638..2667
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
                     /locus_tag="Myc"
                     /ApEinfo_label="Myc"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="Myc"
     CDS             2671..2817
                     /codon_start=1
                     /product="transmembrane domain from platelet derived
                     growth factor receptor beta"
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLW
                     QKKPR"
                     /locus_tag="PDGFR-beta TM domain"
                     /ApEinfo_label="PDGFR-beta TM domain"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="PDGFR-beta TM domain"
     misc_feature    2849..3094
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="WPRE3"
     misc_feature    3108..3311
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="BGH-pA"
     misc_feature    3334..3474
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="R-ITR"
     rep_origin      3566..3872
                     /locus_tag="f1 Origin"
                     /ApEinfo_label="f1 Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="f1 Origin"
     misc_feature    4391..5248
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="AmpR"
     rep_origin      5399..6066
                     /locus_tag="pUC ori"
                     /ApEinfo_label="pUC ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgaaagg aaagagcttg ctttcaacct caaaagctag
      181 gaggaaaggg ctctgaaatt tgctcagaat tcccaattca ccattagcct gtttcttcct
      241 ttagcctcaa ggcattctcc gctttttgaa aagatgttaa gaaattcagt cacaatagag
      301 agcctagttt tgaacatgtt tcactcggtc cattgaggtc tgggctccag cctttgtgtg
      361 gggtgaattg agctgagcgg ctagctggtt ggaggaaagg aaagagcttg ctttcaacct
      421 caaaagctag gaggaaaggg ctctgaaatt tgctcagaat tcccaattca ccattagcct
      481 gtttcttcct ttagcctcaa ggcattctcc gctttttgaa aagatgttaa gaaattcagt
      541 cacaatagag agcctagttt tgaacatgtt tcactcggtc cattgaggtc tgggctccag
      601 cctttgtgtg gggtgaattg agctgagcgg ctagctggtt ggaggaaagg aaagagcttg
      661 ctttcaacct caaaagctag gaggaaaggg ctctgaaatt tgctcagaat tcccaattca
      721 ccattagcct gtttcttcct ttagcctcaa ggcattctcc gctttttgaa aagatgttaa
      781 gaaattcagt cacaatagag agcctagttt tgaacatgtt tcactcggtc cattgaggtc
      841 tgggctccag cctttgtgtg gggtgaattg agctgagcgg ctagctggtt ggaggagctc
      901 gggctgggca taaaagtcag ggcagagcca tctattgctt acatttgctt ctgggatcca
      961 gatctttcga agctagcgct accggtcgcc accatggaga cagacacact cctgctatgg
     1021 gtactgctgc tctgggttcc aggttccact ggtgacagat ctgcgaacga caccgtagtt
     1081 gtgggctcga tcatctttac agaagggatt atcgtcgcaa acatggtggc agagatgatt
     1141 gaggcgcata cagaccttaa ggtggttcgc aaactgaacc ttggcggggt caacgttaac
     1201 tttgaagcca ttaaacgcgg aggtgcgaat aatggtattg acatttacgt ggagtacact
     1261 gggcacggtc ttgtggatat tctggggttc ccggagccga acgtctatat caccgccgac
     1321 aagcagaaga acggcatcaa ggcgaacttc aagatccgcc acaacgtgga ggacggcagc
     1381 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1441 cccgacaacc actacctgag ctatcagtcc gtgctgagca aagaccccaa cgagaagcgc
     1501 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catgaacgag
     1561 ctgtacaagg gcggtaccgg agggagcatg agcaagggcg aggagctgtt caccggggtg
     1621 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgcgcggc
     1681 gagggcgagg gcgatgccac caacggcaag ctgaccctga agctcctgtg caccaccggc
     1741 aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcgt gcagtgcttc
     1801 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1861 tacgtccagg agcgcaccat cagcttcaag gacgacggca cctacaagac ccgcgccgag
     1921 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1981 gaggacggca acatcctggg gcacaagctg gagtacaact ttccgccgcc cgcgactact
     2041 gatccagaag gtgcatacga aaccgtgaag aaggagtaca aacgtaaatg gaatattgta
     2101 tggctcaaac cactgggatt caacaatacg tatacgctta ccgttaaaga cgaactggcg
     2161 aaacagtata accttaaaac cttcagtgac ttagcgaaaa tctcggataa gctgattctg
     2221 ggtgcaacga tgttcttttt agaagggccc gatggttacc caggcctgca aaaactgtac
     2281 aatttcaaat tcaagcacac caaaagcatg gacatgggta ttcgctatac cgccattgat
     2341 aataacgaag ttcaggtaat tgatgcctgg gccactgatg gcttgctggt gagccacaaa
     2401 ttaaaaattc tggaggatga taaagcgttc ttcccgccgt attatgctgc ccccatcatc
     2461 cgtcaggatg tcttagataa gcatcctgaa ctgaaggacg tgctgaacaa actcgcgaat
     2521 caaatttcac tggaagaaat gcagaaactg aattacaagg tggacggtga gggtcaggac
     2581 ccagcgaaag tagctaagga gtttttgaaa gagaaaggtt taattctgca ggtcgacgaa
     2641 caaaaactca tctcagaaga ggatctgaat gctgtgggcc aggacacgca ggaggtcatc
     2701 gtggtgccac actccttgcc ctttaaggtg gtggtgatct cagccatcct ggccctggtg
     2761 gtgctcacca tcatctccct tatcatcctc atcatgcttt ggcagaagaa gccacgttag
     2821 ggcgcgccgc ggccgcgaat tcgatatcat aatcaacctc tggattacaa aatttgtgaa
     2881 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2941 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3001 tcctggttag ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     3061 ggggctcggc tgttgggcac tgacaattcc gtggctcgag agatcttcga ctgtgccttc
     3121 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     3181 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     3241 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     3301 tagcaggcat gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
     3361 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     3421 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     3481 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     3541 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3601 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3661 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3721 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     3781 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3841 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt
     3901 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     3961 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     4021 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     4081 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     4141 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     4201 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     4261 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4321 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4381 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     4441 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     4501 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     4561 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     4621 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4681 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4741 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4801 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4861 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4921 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4981 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5041 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5101 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5161 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5221 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5281 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5341 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5401 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5461 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5521 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     5581 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5641 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5701 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5761 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5821 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5881 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5941 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6001 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6061 tgctcacatg t
//
