LOCUS       CN4253_pAAV-3xc2_eHGT_380h-minBglobin-SYFP2-WPRE3-BGHpA        4858 bp    DNA     circular UNA 24-AUG-2023
DEFINITION  .
ACCESSION   urn.local...xk-ev0gxi3
VERSION     urn.local...xk-ev0gxi3
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Comment-START##                                                  
            Comment :: rAAV-ESYN-SYFP2-WPRE3-BGHpA                             
                       clone #8-1 correct , 8-2 backup                         
            ##Comment-END##                                                    
            ##Imported_From-START##                                            
            Path     :: C:\VNTI Database                                       
            Filename :: _exdata.ma4                                            
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
     Enhancer        156..782
                     /created_by="ximena.opitzaraya"
                     /modified_by="adminkellyyun"
                     /label="3xCore2(380h)"
     promoter        789..841
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="adminkellyyun"
                     /label="Beta Globin minimal promoter"
     CDS             882..1601
                     /modified_by="adminkellyyun"
                     /label="SYFP2"
     regulatory      1636..1881
                     /modified_by="adminkellyyun"
                     /label="WPRE3"
     polyA_site      1895..2098
                     /modified_by="adminkellyyun"
                     /label="BGH-pA"
     misc_feature    2121..2261
                     /label="R-ITR"
     rep_origin      2353..2659
                     /label="f1 Origin"
     misc_feature    3178..4035
                     /label="AmpR"
     rep_origin      4186..4853
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtagccc aagagtaggg ggtggtgctg
      181 tgggccactg tgcagaggcc aggactgtga atgaagtggg cactgctcct gccccctccc
      241 aggcctgcct ctggccaggc cagcctccat cccggcattg tctcaggcat cttgggcagg
      301 gttcccagag ctggctgaac caggggcctg gccaaacagc cctcccctgc cctcccattc
      361 ctctagccca agagtagggg gtggtgctgt gggccactgt gcagaggcca ggactgtgaa
      421 tgaagtgggc actgctcctg ccccctccca ggcctgcctc tggccaggcc agcctccatc
      481 ccggcattgt ctcaggcatc ttgggcaggg ttcccagagc tggctgaacc aggggcctgg
      541 ccaaacagcc ctcccctgcc ctcccattcc tctagcccaa gagtaggggg tggtgctgtg
      601 ggccactgtg cagaggccag gactgtgaat gaagtgggca ctgctcctgc cccctcccag
      661 gcctgcctct ggccaggcca gcctccatcc cggcattgtc tcaggcatct tgggcagggt
      721 tcccagagct ggctgaacca ggggcctggc caaacagccc tcccctgccc tcccattcct
      781 ctgagctcgg gctgggcata aaagtcaggg cagagccatc tattgcttac atttgcttct
      841 gggatccaga tctttcgaag ctagcgctac cggtcgccac catggtgagc aagggcgagg
      901 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      961 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc
     1021 tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct
     1081 acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1141 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1201 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1261 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1321 acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
     1381 agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca
     1441 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca
     1501 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1561 ccgccgggat cactctcggc atggacgagc tgtacaagta agtcgacggc gcgccgcggc
     1621 cgcgaattcg atatcataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     1681 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     1741 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttagttc
     1801 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     1861 tgggcactga caattccgtg gctcgagaga tcttcgactg tgccttctag ttgccagcca
     1921 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     1981 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     2041 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgca
     2101 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     2161 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     2221 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     2281 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     2341 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2401 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2461 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2521 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     2581 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     2641 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     2701 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     2761 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     2821 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2881 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2941 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     3001 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     3061 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3121 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3181 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3241 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     3301 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     3361 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3421 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3481 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3541 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3601 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3661 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3721 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3781 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3841 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3901 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3961 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4021 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     4081 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4141 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4201 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4261 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4321 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     4381 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4441 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4501 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4561 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4621 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4681 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4741 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4801 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
