LOCUS       CN3318-pAAV-3xcore2_eHGT_390m-minBglobin-SYFP2-4x2C-WPRE3-BGHpA        5190 bp    DNA     circular     24-AUG-2023
DEFINITION  .
ACCESSION   urn.local...10-bsfuej6
VERSION     urn.local...10-bsfuej6
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Comment-START## Comment :: rAAV-ESYN-SYFP2-WPRE3-BGHpA           
            clone #8-1 correct , 8-2 backup ##Comment-END##                    
            ##Imported_From-START## Path     :: C:\VNTI Database Filename ::   
            _exdata.ma4 ##Imported_From-END##                                  
                                                                               
            ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /vntifkey=21
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     Enhancer        145..894
                     /created_by="ximena.opitzaraya"
                     /locus_tag="3xcore2_eHGT_390m"
                     /ApEinfo_label="3xcore2_eHGT_390m"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="3xcore2_eHGT_390m"
     misc_feature    901..953
                     /locus_tag="Beta Globin minimal promoter"
                     /ApEinfo_label="Beta Globin minimal promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta Globin minimal promoter"
     CDS             994..1713
                     /locus_tag="SYFP2"
                     /ApEinfo_label="SYFP2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /modified_by="adminkellyyun"
                     /label="SYFP2"
     misc_feature    1736..1756
                     /locus_tag="miR128 recognition sequence"
                     /ApEinfo_label="miR128 recognition sequence"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="miR128 recognition sequence"
     misc_feature    1762..1782
                     /locus_tag="miR128 recognition sequence(1)"
                     /ApEinfo_label="miR128 recognition sequence"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="miR128 recognition sequence(1)"
     misc_feature    1788..1808
                     /locus_tag="miR128 recognition sequence(2)"
                     /ApEinfo_label="miR128 recognition sequence"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="miR128 recognition sequence(2)"
     misc_feature    1814..1834
                     /locus_tag="miR128 recognition sequence(3)"
                     /ApEinfo_label="miR128 recognition sequence"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="miR128 recognition sequence(3)"
     misc_feature    1843..1865
                     /locus_tag="miR221 recognition sequence"
                     /ApEinfo_label="miR221 recognition sequence"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="miR221 recognition sequence"
     misc_feature    1871..1893
                     /locus_tag="miR221 recognition sequence(1)"
                     /ApEinfo_label="miR221 recognition sequence"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="miR221 recognition sequence(1)"
     misc_feature    1899..1921
                     /locus_tag="miR221 recognition sequence(2)"
                     /ApEinfo_label="miR221 recognition sequence"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="miR221 recognition sequence(2)"
     misc_feature    1927..1949
                     /locus_tag="miR221 recognition sequence(3)"
                     /ApEinfo_label="miR221 recognition sequence"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="miR221 recognition sequence(3)"
     misc_feature    1968..2213
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="WPRE3"
     misc_feature    2227..2430
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="BGH-pA"
     misc_feature    2453..2593
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="R-ITR"
     rep_origin      2685..2991
                     /locus_tag="f1 Origin"
                     /ApEinfo_label="f1 Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="f1 Origin"
     misc_feature    3510..4367
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="AmpR"
     rep_origin      4518..5185
                     /locus_tag="pUC ori"
                     /ApEinfo_label="pUC ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgaaagg aaagagcttg ctttcaacct caaaagctag
      181 gaggaaaggg ctctgaaatt tgctcagaat tcccaattca ccattagcct gtttcttcct
      241 ttagcctcaa ggcattctcc gctttttgaa aagatgttaa gaaattcagt cacaatagag
      301 agcctagttt tgaacatgtt tcactcggtc cattgaggtc tgggctccag cctttgtgtg
      361 gggtgaattg agctgagcgg ctagctggtt ggaggaaagg aaagagcttg ctttcaacct
      421 caaaagctag gaggaaaggg ctctgaaatt tgctcagaat tcccaattca ccattagcct
      481 gtttcttcct ttagcctcaa ggcattctcc gctttttgaa aagatgttaa gaaattcagt
      541 cacaatagag agcctagttt tgaacatgtt tcactcggtc cattgaggtc tgggctccag
      601 cctttgtgtg gggtgaattg agctgagcgg ctagctggtt ggaggaaagg aaagagcttg
      661 ctttcaacct caaaagctag gaggaaaggg ctctgaaatt tgctcagaat tcccaattca
      721 ccattagcct gtttcttcct ttagcctcaa ggcattctcc gctttttgaa aagatgttaa
      781 gaaattcagt cacaatagag agcctagttt tgaacatgtt tcactcggtc cattgaggtc
      841 tgggctccag cctttgtgtg gggtgaattg agctgagcgg ctagctggtt ggaggagctc
      901 gggctgggca taaaagtcag ggcagagcca tctattgctt acatttgctt ctgggatcca
      961 gatctttcga agctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
     1021 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     1081 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc
     1141 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcgtg
     1201 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1261 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1321 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1381 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1441 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
     1501 cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc
     1561 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc
     1621 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1681 atcactctcg gcatggacga gctgtacaag taagtcgacg gcgcgccgcg gccttaaaga
     1741 gaccggttca ctgtgacagt aaaagagacc ggttcactgt gagaatgaaa gagaccggtt
     1801 cactgtgatc ggaaaagaga ccggttcact gtgagcggcc ttgaaaccca gcagacaatg
     1861 tagctcagta gaaacccagc agacaatgta gctgaatgga aacccagcag acaatgtagc
     1921 ttcggagaaa cccagcagac aatgtagctg tcgacgaatt cgatatcata atcaacctct
     1981 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     2041 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     2101 tttctcctcc ttgtataaat cctggttagt tcttgccacg gcggaactca tcgccgcctg
     2161 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggctcgaga
     2221 gatcttcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     2281 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     2341 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     2401 gggaggattg ggaagacaat agcaggcatg cacgtgcgga ccgagcggcc gcaggaaccc
     2461 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     2521 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     2581 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     2641 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     2701 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     2761 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     2821 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     2881 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     2941 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     3001 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     3061 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     3121 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     3181 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     3241 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     3301 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     3361 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3421 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3481 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3541 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3601 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3661 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3721 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     3781 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3841 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3901 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3961 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4021 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4081 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4141 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4201 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4261 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4321 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     4381 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     4441 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4501 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4561 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4621 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4681 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4741 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4801 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4861 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4921 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4981 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5041 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5101 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5161 tggccttttg ctggcctttt gctcacatgt
//
