LOCUS       Exported                3968 bp ds-DNA     circular SYN 05-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSC101-AmpR EV
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Mariliis
  TITLE     Direct Submission
  JOURNAL   Exported Monday, August 28, 2023 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             complement(196..1056)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1057..1161)
                     /gene="bla"
                     /note="AmpR promoter"
     misc_feature    1213..1240
                     /note="rrnB T2 terminator"
     misc_feature    1361..1366
                     /note="-35"
     misc_feature    1380..1385
                     /note="-10"
     misc_feature    1395
                     /note="dnaKp1"
                     /note="transcription start of promoter P1 (rpoH dependent)"
     misc_feature    1446..1479
                     /note="Tet_prom_to_SD"
     promoter        1448..1476
                     /note="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_feature    1480..1481
                     /note="trnscr_start"
                     /note="Tac promoter transcription start site"
     terminator      1531..1625
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     rep_origin      2117..2339
                     /note="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2387..3337
                     /codon_start=1
                     /gene="rep101"
                     /product="protein needed for replication with the pSC101 
                     origin"
                     /note="Rep101"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(3872..3958)
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agcttgcatg cccaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat
       61 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
      121 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
      181 gcgtccggcg tagagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
      241 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
      301 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
      361 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
      421 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
      481 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
      541 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
      601 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
      661 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
      721 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
      781 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
      841 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
      901 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
      961 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     1021 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     1081 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     1141 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     1201 cattattatc ataaaaggcc atccgtcagg atggccttct atgaatgcct tggctgcgat
     1261 tcattcttta tatgaataaa attgctgtca attttacgtc ttgtcctgcc atatcgcgaa
     1321 atttctgcgc aaaagcacaa aaaatttttg catctccccc ttgatgacgt ggtttacgac
     1381 cccatttagt agtcaaccgc agtgagtgag tctgacgagg ccctttcgtc ttcaagaatt
     1441 ctcatgtttg acagcttatc atcgataagc tttaatgcgc catggatcga tagctggtcg
     1501 acaggactct ggggttcgag agctcgcttg gactcctgtt gatagatcca gtaatgacct
     1561 cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg
     1621 agaatccaag cactagggac agtaagacgg gtaagcctgt tgatgatacc gctgccttac
     1681 tgggtgcatt agccagtctg aatgacctgt cacgggataa tccgaagtgg tcagactgga
     1741 aaatcagagg gcaggaactg ctgaacagca aaaagtcaga tagcaccaca tagcagaccc
     1801 gccataaaac gccctgagaa gcccgtgacg ggcttttctt gtattatggg tagtttcctt
     1861 gcatgaatcc ataaaaggcg cctgtagtgc catttacccc cattcactgc cagagccgtg
     1921 agcgcagcga actgaatgtc acgaaaaaga cagcgactca ggtgcctgat ggtcggagac
     1981 aaaaggaata ttcagcgatt tgcccgagct tgcgagggtg ctacttaagc ctttagggtt
     2041 ttaaggtctg ttttgtagag gagcaaacag cgtttgcgac atccttttgt aatactgcgg
     2101 aactgactaa agtagtgagt tatacacagg gctgggatct attcttttta tcttttttta
     2161 ttctttcttt attctataaa ttataaccac ttgaatataa acaaaaaaaa cacacaaagg
     2221 tctagcggaa tttacagagg gtctagcaga atttacaagt tttccagcaa aggtctagca
     2281 gaatttacag atacccacaa ctcaaaggaa aaggactagt aattatcatt gactagccca
     2341 tctcaattgg tatagtgatt aaaatcacct agaccaattg agatgtatgt ctgaattagt
     2401 tgttttcaaa gcaaatgaac tagcgattag tcgctatgac ttaacggagc atgaaaccaa
     2461 gctaatttta tgctgtgtgg cactactcaa ccccacgatt gaaaacccta caaggaaaga
     2521 acggacggta tcgttcactt ataaccaata cgctcagatg atgaacatca gtagggaaaa
     2581 tgcttatggt gtattagcta aagcaaccag agagctgatg acgagaactg tggaaatcag
     2641 gaatcctttg gttaaaggct ttgagatttt ccagtggaca aactatgcca agttctcaag
     2701 cgaaaaatta gaattagttt ttagtgaaga gatattgcct tatcttttcc agttaaaaaa
     2761 attcataaaa tataatctgg aacatgttaa gtcttttgaa aacaaatact ctatgaggat
     2821 ttatgagtgg ttattaaaag aactaacaca aaagaaaact cacaaggcaa atatagagat
     2881 tagccttgat gaatttaagt tcatgttaat gcttgaaaat aactaccatg agtttaaaag
     2941 gcttaaccaa tgggttttga aaccaataag taaagattta aacacttaca gcaatatgaa
     3001 attggtggtt gataagcgag gccgcccgac tgatacgttg attttccaag ttgaactaga
     3061 tagacaaatg gatctcgtaa ccgaacttga gaacaaccag ataaaaatga atggtgacaa
     3121 aataccaaca accattacat cagattccta cctacgtaac ggactaagaa aaacactaca
     3181 cgatgcttta actgcaaaaa ttcagctcac cagttttgag gcaaaatttt tgagtgacat
     3241 gcaaagtaag catgatctca atggttcgtt ctcatggctc acgcaaaaac aacgaaccac
     3301 actagagaac atactggcta aatacggaag gatctgaggt tcttatggct cttgtatcta
     3361 tcagtgaagc atcaagacta acaaacaaaa gtagaacaac tgttcaccgt tagatatcaa
     3421 agggaaaact gtccatatgc acagatgaaa acggtgtaaa aaagatagat acatcagagc
     3481 ttttacgagt ttttggtgca tttaaagctg ttcaccatga acagatcgac aatgtaacag
     3541 atgaacagca tgtaacacct aatagaacag gtgaaaccag taaaacaaag caactagaac
     3601 atgaaattga acacctgaga caacttgtta cagctcaaca gtcacacata gacagcctga
     3661 aacaggcgat gctgcttatc gaatcaaagc tgccgacaac acgggagcca gtgacgcctc
     3721 ccgtggggaa aaaatcatgg caattctgga agaaatagcg ctttcagccg gcaaacctga
     3781 agccggatct gcgattctga taacaaacta gcaacaccag aacagcccgt ttgcgggcag
     3841 caaaacccgt accctaggtc tagggcggcg gatttgtcct actcaggaga gcgttcaccg
     3901 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
     3961 gcctctag
//
