LOCUS       Exported                4875 bp ds-DNA     circular SYN 23-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSC101-GFPmut2(Delta-mScarlet)
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Mariliis
  TITLE     Direct Submission
  JOURNAL   Exported Monday, August 28, 2023 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             complement(196..1056)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1057..1161)
                     /gene="bla"
                     /note="AmpR promoter"
     misc_feature    1213..1240
                     /note="rrnB T2 terminator"
     misc_feature    1361..1366
                     /note="-35"
     misc_feature    1380..1385
                     /note="-10"
     misc_feature    1395
                     /note="dnaKp1"
                     /note="transcription start of promoter P1 (rpoH dependent)"
     misc_feature    1446..1511
                     /note="Tet_prom_to_SD"
     promoter        1448..1476
                     /note="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1540..2265
                     /codon_start=1
                     /product="GFP"
                     /note="GFPmut2"
                     /translation="MKGSSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYIMADKQKDG
                     IKVNFKIRHNIEDGSVQLADHYQRNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITHGMDELYK"
     CDS             1552..1559
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /note="yeGFP"
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SK"
     misc_feature    2296..2388
                     /note="trnscr_start"
                     /note="Tac promoter transcription start site"
     CDS             2296..2319
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /translation="GMDELYK"
     CDS             2330..2353
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /note="8xHis"
                     /translation="HHHHHHHH"
     terminator      2438..2532
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     rep_origin      3024..3246
                     /note="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             3294..4244
                     /codon_start=1
                     /gene="rep101"
                     /product="protein needed for replication with the pSC101 
                     origin"
                     /note="Rep101"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(4779..4865)
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agcttgcatg cccaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat
       61 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
      121 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
      181 gcgtccggcg tagagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
      241 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
      301 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
      361 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
      421 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
      481 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
      541 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
      601 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
      661 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
      721 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
      781 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
      841 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
      901 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
      961 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     1021 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     1081 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     1141 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     1201 cattattatc ataaaaggcc atccgtcagg atggccttct atgaatgcct tggctgcgat
     1261 tcattcttta tatgaataaa attgctgtca attttacgtc ttgtcctgcc atatcgcgaa
     1321 atttctgcgc aaaagcacaa aaaatttttg catctccccc ttgatgacgt ggtttacgac
     1381 cccatttagt agtcaaccgc agtgagtgag tctgacgagg ccctttcgtc ttcaagaatt
     1441 ctcatgtttg acagcttatc atcgataagc tttaatgcgg tagtttatca cagttaaatt
     1501 gctaacgcag tccggggaat tcaggcttgg aggaacaata tgaaaggatc ctctaaaggt
     1561 gaagaattat tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     1621 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     1681 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
     1741 gcgtatggtc ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
     1801 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     1861 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
     1921 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
     1981 tataactcac acaaagtata catcatggca gacaaacaaa aggatggaat caaagttaac
     2041 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacga
     2101 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
     2161 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     2221 acagctgctg ggattacaca tggtatggat gaattgtaca aataaccgcc aggaggaaca
     2281 atatggtgag caaggggcat ggacgagctg tacaagtaaa agcctcgagc accaccacca
     2341 ccaccaccac cactaattga ttaataccta ggctgctaaa caaagcccat ggatcgatag
     2401 ctggtcgaca ggactctggg gttcgagagc tcgcttggac tcctgttgat agatccagta
     2461 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt
     2521 attggtgaga atccaagcac tagggacagt aagacgggta agcctgttga tgataccgct
     2581 gccttactgg gtgcattagc cagtctgaat gacctgtcac gggataatcc gaagtggtca
     2641 gactggaaaa tcagagggca ggaactgctg aacagcaaaa agtcagatag caccacatag
     2701 cagacccgcc ataaaacgcc ctgagaagcc cgtgacgggc ttttcttgta ttatgggtag
     2761 tttccttgca tgaatccata aaaggcgcct gtagtgccat ttacccccat tcactgccag
     2821 agccgtgagc gcagcgaact gaatgtcacg aaaaagacag cgactcaggt gcctgatggt
     2881 cggagacaaa aggaatattc agcgatttgc ccgagcttgc gagggtgcta cttaagcctt
     2941 tagggtttta aggtctgttt tgtagaggag caaacagcgt ttgcgacatc cttttgtaat
     3001 actgcggaac tgactaaagt agtgagttat acacagggct gggatctatt ctttttatct
     3061 ttttttattc tttctttatt ctataaatta taaccacttg aatataaaca aaaaaaacac
     3121 acaaaggtct agcggaattt acagagggtc tagcagaatt tacaagtttt ccagcaaagg
     3181 tctagcagaa tttacagata cccacaactc aaaggaaaag gactagtaat tatcattgac
     3241 tagcccatct caattggtat agtgattaaa atcacctaga ccaattgaga tgtatgtctg
     3301 aattagttgt tttcaaagca aatgaactag cgattagtcg ctatgactta acggagcatg
     3361 aaaccaagct aattttatgc tgtgtggcac tactcaaccc cacgattgaa aaccctacaa
     3421 ggaaagaacg gacggtatcg ttcacttata accaatacgc tcagatgatg aacatcagta
     3481 gggaaaatgc ttatggtgta ttagctaaag caaccagaga gctgatgacg agaactgtgg
     3541 aaatcaggaa tcctttggtt aaaggctttg agattttcca gtggacaaac tatgccaagt
     3601 tctcaagcga aaaattagaa ttagttttta gtgaagagat attgccttat cttttccagt
     3661 taaaaaaatt cataaaatat aatctggaac atgttaagtc ttttgaaaac aaatactcta
     3721 tgaggattta tgagtggtta ttaaaagaac taacacaaaa gaaaactcac aaggcaaata
     3781 tagagattag ccttgatgaa tttaagttca tgttaatgct tgaaaataac taccatgagt
     3841 ttaaaaggct taaccaatgg gttttgaaac caataagtaa agatttaaac acttacagca
     3901 atatgaaatt ggtggttgat aagcgaggcc gcccgactga tacgttgatt ttccaagttg
     3961 aactagatag acaaatggat ctcgtaaccg aacttgagaa caaccagata aaaatgaatg
     4021 gtgacaaaat accaacaacc attacatcag attcctacct acgtaacgga ctaagaaaaa
     4081 cactacacga tgctttaact gcaaaaattc agctcaccag ttttgaggca aaatttttga
     4141 gtgacatgca aagtaagcat gatctcaatg gttcgttctc atggctcacg caaaaacaac
     4201 gaaccacact agagaacata ctggctaaat acggaaggat ctgaggttct tatggctctt
     4261 gtatctatca gtgaagcatc aagactaaca aacaaaagta gaacaactgt tcaccgttag
     4321 atatcaaagg gaaaactgtc catatgcaca gatgaaaacg gtgtaaaaaa gatagataca
     4381 tcagagcttt tacgagtttt tggtgcattt aaagctgttc accatgaaca gatcgacaat
     4441 gtaacagatg aacagcatgt aacacctaat agaacaggtg aaaccagtaa aacaaagcaa
     4501 ctagaacatg aaattgaaca cctgagacaa cttgttacag ctcaacagtc acacatagac
     4561 agcctgaaac aggcgatgct gcttatcgaa tcaaagctgc cgacaacacg ggagccagtg
     4621 acgcctcccg tggggaaaaa atcatggcaa ttctggaaga aatagcgctt tcagccggca
     4681 aacctgaagc cggatctgcg attctgataa caaactagca acaccagaac agcccgtttg
     4741 cgggcagcaa aacccgtacc ctaggtctag ggcggcggat ttgtcctact caggagagcg
     4801 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
     4861 atttgatgcc tctag
//
