LOCUS       1529_pAAV-CB-FKB        6093 bp DNA     circular SYN 12-SEP-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pAVV-CB-EGF
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 28, 2023 from SnapGene Viewer 7.0.2
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..6093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     stem_loop       1..130
                     /label=5'-ITR
                     /note="vntifkey=39"
                     /note="color: #0000ff"
     enhancer        196..321
                     /label=Partial CMV enhancer
                     /label=Partial\CMV\enhancer
                     /note="vntifkey=9"
                     /note="color: #000080; direction: RIGHT"
     promoter        322..581
                     /label=CB promoter
                     /label=CB\promoter
                     /note="vntifkey=29"
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    748..772
                     /label=sequencing primer_5'
                     /label=sequencing\primer_5'
                     /note="vntifkey=21"
                     /note="color: #3366cc"
     intron          806..938
                     /label=SV40-Promega intron
                     /label=SV40-Promega\intron
                     /note="vntifkey=15"
                     /note="color: #0000ff"
     misc_feature    956..1279
                     /label=FKBP12
                     /note="vntifkey=21"
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    1292..2254
                     /label=BirA*
                     /note="vntifkey=21"
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    2258..2284
                     /label=HA tag
                     /label=HA\tag
                     /note="vntifkey=21"
                     /note="color: #3366cc"
     misc_feature    2285..2293
                     /label=stop codon
                     /label=stop\codon
                     /note="vntifkey=21"
                     /note="color: #3366cc"
     enhancer        2304..2829
                     /label=WPRE enhancer
                     /label=WPRE
                     /note="vntifkey=9"
                     /note="color: #000080; direction: RIGHT"
     misc_feature    2375..2397
                     /label=AH_0001
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2377..2402
                     /label=sequencing primer 3'
                     /label=sequencing\primer\3'
                     /note="vntifkey=21"
                     /note="color: #3366cc"
     misc_feature    2552..2571
                     /label=AH_0104
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(2604..2623)
                     /label=AH_0002
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    2604..2623
                     /label=sequencing primer 3'
                     /label=sequencing\primer\3'
                     /note="vntifkey=21"
                     /note="color: #3366cc"
     misc_feature    complement(2752..2771)
                     /label=AH_0105
                     /note="color: #a6acb3; direction: LEFT"
     polyA_site      2852..3066
                     /label=BGH polyA
                     /label=BGH\polyA
                     /note="vntifkey=26"
                     /note="color: #000000"
     misc_feature    3108..3153
                     /label=Additional AVV sequences
                     /label=Additional\AVV\sequences
                     /note="vntifkey=21"
                     /note="color: #3366cc"
     stem_loop       3108..3153
                     /label=3'-ITR
                     /note="vntifkey=39"
                     /note="color: #0000ff"
     misc_feature    4046..4903
                     /label=Amp-R
                     /note="vntifkey=21"
                     /note="color: #66cc99; direction: RIGHT"
     rep_origin      5077..5665
                     /direction=RIGHT
                     /label=Origin
                     /note="vntifkey=33"
                     /note="color: #ff9966"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcgccc ttaagctagt atgccaagta cgccccctat tgacgtcaat
      241 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      301 tggcagtaca tctactcgag gccacgttct gcttcactct ccccatctcc cccccctccc
      361 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
      421 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag
      481 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
      541 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagcgg gatcagaatg
      601 atctgatatc atcgatgaat tcgagctcgg tacccgggga tcctctagag tcgaggaact
      661 gaaaaaccag aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga
      721 tccggtggtg gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggg
      781 aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga
      841 ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat
      901 tggtcttact gacatccact ttgcctttct ctccacaggt gtccaggcgg ccgccatggg
      961 agtgcaggtg gagaccatct ctcctggaga cgggcgcacc ttcccaaagc gcggccagac
     1021 ctgcgtggtg cactacacgg ggatgcttga agatggaaag aaatttgatt cctctcggga
     1081 cagaaacaag ccttttaagt ttacactagg caagcaggag gtgatccgag gctgggagga
     1141 aggggtagcc cagatgagtg tgggtcagag agccaaactg ataatctcct cagactatgc
     1201 ctatggagcc accgggcacc caggcatcat cccaccacat gccactcttg tttttgatgt
     1261 ggagcttcta aaactggaag gaggatcggg aatgaaggac aacaccgtgc ccctgaagct
     1321 gatcgccctg ctggccaacg gcgagttcca ctctggcgag cagctgggag agaccctggg
     1381 aatgagcaga gccgccatca acaagcacat ccagacactg agagactggg gagtggacgt
     1441 gttcaccgtg cctggcaagg gctacagcct gcctgagcct atccagctgc tgaacgccaa
     1501 gcagatcctg ggacagctgg atggcggaag cgtggccgtg ctgcctgtga tcgactccac
     1561 caatcagtac ctgctggaca gaatcggaga gctgaagtcc ggcgacgcct gcatcgccga
     1621 gtaccagcag gctggcagag gaggcagagg acggaagtgg ttcagcccat tcggagccaa
     1681 cctgtacctg tccatgttct ggagactgga gcagggacct gctgctgcca tcggactgag
     1741 tctggtgatc ggaatcgtga tggccgaggt gctgagaaag ctgggagccg acaaggtgag
     1801 agtgaagtgg cctaatgacc tgtacctcca ggaccgcaag ctggctggca tcctggtgga
     1861 gctgacaggc aagacaggcg atgccgctca gatcgtgatc ggagccggaa tcaacatggc
     1921 catgagaaga gtggaggaga gcgtggtgaa ccagggctgg atcaccctgc aggaggctgg
     1981 catcaacctg gaccggaaca ccctggccgc catgctgatc agagagctga gagccgctct
     2041 ggagctgttc gagcaggagg gactggctcc ttacctgagc agatgggaga agctggacaa
     2101 cttcatcaac agacctgtga agctgatcat cggcgacaag gaaatcttcg gcatctccag
     2161 aggaatcgac aagcagggag ctctgctgct ggagcaggac ggaatcatca agccctggat
     2221 gggcggagaa atctccctga gaagcgcaga gaaggcttac ccttacgatg taccggatta
     2281 cgcataataa taagcttgga tccaatcaac ctctggatta caaaatttgt gaaagattga
     2341 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     2401 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     2461 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     2521 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     2581 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     2641 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     2701 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     2761 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     2821 ctctgcggcc tcttccgcgt cttcgagatc tgcctcgact gtgccttcta gttgccagcc
     2881 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     2941 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     3001 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     3061 tggggactcg agttaagggc gaattcccga taaggatctt cctagagcat ggctacgtag
     3121 ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg gagttggcca
     3181 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     3241 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagccttaat taacctaatt
     3301 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     3361 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     3421 gcccttccca acagttgcgc agcatgaatg gcgaatggga cgcgccctgt agcggcgcat
     3481 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     3541 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3601 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3661 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3721 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3781 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     3841 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3901 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3961 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4021 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4081 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4141 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4201 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4261 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4321 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4381 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4441 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4501 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4561 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4621 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4681 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4741 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4801 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4861 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4921 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4981 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5041 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5101 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5161 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5221 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5281 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5341 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5401 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5461 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5521 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5581 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5641 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5701 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     5761 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     5821 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     5881 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     5941 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     6001 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     6061 ctatgaccat gattacgcca gatttaatta agg
//
