LOCUS       Exported                9695 bp ds-DNA     circular SYN 28-AUG-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   GML
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 28, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     76..96
                     /label=sGML0002C
     terminator      90..133
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator (B0062)"
     primer_bind     120..157
                     /label=oGML0091_f
                     /note="amplify GFP with BbsI(1/2 scar, out) and BsaI(1/6
                     scar, in) for pMBIS derivative"
     misc_feature    136..157
                     /label=BBa prefix
     misc_feature    174..177
                     /label=4bp_scar1
     misc_feature    178..200
                     /label=spacer_SP1_1-4
     misc_feature    201..204
                     /label=4bp_scar1
     promoter        205..221
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    224..248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_RNA        270..347
                     /label=SarJ
     misc_feature    348..350
                     /label=3bp_scar2
     misc_feature    351..374
                     /label=RBS
                     /note="TIR = 50033.31"
     CDS             375..1658
                     /codon_start=1
                     /product="HMG-CoA reductase
                     
                     "
                     /label=HMGR
                     /translation="MSMQSLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEE
                     VANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAIDT
                     FPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASVVK
                     VQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLATGND
                     TRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPIAKAS
                     LELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGAKGDEI
                     AKVAEALKKEPRANTQAAEHILQEIRQQ"
     primer_bind     641..660
                     /label=oGML0252
                     /note="sequence pGML0149"
     primer_bind     1426..1447
                     /label=oGML0253
                     /note="sequence pGML0149"
     primer_bind     1659..1681
                     /label=oGML0204
                     /note="sequence 4bp_scar2"
     misc_feature    1659..1661
                     /label=3bp_scar9
     misc_feature    1662..1681
                     /label=spacer
     terminator      1682..1733
                     /label=L3S1P52
     misc_feature    1734..1753
                     /label=spacer
     misc_feature    1754..1757
                     /label=4bp_scar2
     misc_feature    1766..1781
                     /label=spacer_SP1_1-4
     misc_feature    1782..1785
                     /label=4bp_scar4
     promoter        1786..1802
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1805..1829
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    1851..1925
                     /label=RiboJ53
                     /note="Geneious type: riboj"
     misc_feature    1925..1927
                     /label=3bp_scar2
     RBS             1928..1957
                     /label=RBS
                     /note="strength = 31338"
     CDS             1958..3559
                     /codon_start=1
                     /product="ent-copalyl diphosphate"
                     /label=Cyc_A07
                     /db_xref="UniProtKB/Swiss-Prot:D8L2U6"
                     /db_xref="Refseq:ACO31276.1"
                     /translation="MLEVPAQPTPAPREAEAAALLAATVADPWGLVAPSVYDTARLVSL
                     APWLDGHRERLGYLVKEQNQDGSWGAPDGYGLVPTLSAVEALLTELARTDSGAPHPPHD
                     DLAAACAGGLGALQDGLLAGPVPDTIGVEFVAPSLLADINTRLAALTEQAPGKLGAWSG
                     TTLTSPAPDLDGALLAGVREMTEQAPLPEKLWHTLEAITRDGTRGARPHEGAPPHNGSV
                     GCSPAATAAWLGASPDPAAPGVAYLRDVQARFGGPVPSITPIVYFEQAWVLNSLAASGL
                     RYEAPAALLDSLEAGLTDEGIAAAPGLPSDSDDTAAVLFALAQHGRTHRPDSLMHFRRD
                     GYFSCFGVERTPSTSTNAHILEALGHHVTVRPDDAGRYGAEIRMISDWLLDNQLPDGSW
                     MDKWHASPYYATACCALALAEFGGPSARAAVDRAAAWALETQRADGSWGRWQGTTEETA
                     YMVQLLMRTRTPGSPGTVARSAARGCDALLAHDDPASYPGLWHDKDIYAPVTVIRAARL
                     AALALGGAESAASGGA"
     primer_bind     2355..2372
                     /label=E6
     primer_bind     3560..3580
                     /label=oGML0207
                     /note="sequence 4bp_scar5"
     misc_feature    3560..3562
                     /label=3bp_scar9
     misc_feature    3563..3582
                     /label=spacer
                     /note="random 20bp spacer"
     terminator      3583..3639
                     /label=L3S2P11
     misc_feature    3640..3659
                     /label=spacer
                     /note="random 20bp spacer"
     misc_feature    3660..3663
                     /label=4bp_scar5
     promoter        3664..3680
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3683..3707
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(3708..3728)
                     /label=oGML0114
                     /note="sequence T7-LacO scar5/6"
     misc_feature    3729..3804
                     /label=BydvJ
                     /note="Geneious type: riboj"
     misc_feature    3729..3730
                     /label=3bp_scar7
     misc_feature    3804..3806
                     /label=3bp_scar2
     RBS             3807..3833
                     /label=RBS
                     /note="strength = 55826"
     CDS             3834..6113
                     /codon_start=1
                     /product="ent-sandaracopimara-8(14),15-diene"
                     /label=Cyc_B15
                     /db_xref="UniProtKB/Swiss-Prot:Q2QQJ5"
                     /db_xref="Refseq:ABH10735.1"
                     /translation="MHSKELQAKIRRQLQRVELSPSLYDTAWVAMVPERSSSQAPCYPQ
                     CIEWILQNQHDDGSWGINSSSLSVNKDILLSTLACVVALKKWNAGSYHIKRGLNFVGRN
                     FSVAMDVQNIAPVGFNVTFSGLITLASGMGLQLPVWQTDIDEIFHLRKIELERDSGGTI
                     SARKAFMAYVAEGFGSLQDWDQVMAYQRKNGSLFNSPSTTAAAAIHTFNDRTLNYLDSL
                     TNKFGGPVPAMYPQNIYSQLCTVDALERTGISQKFAREIRDILDTTYRSWLHNEEEVML
                     DIPTCAMAFRLLRTHGYDITSDEMAHFSEQSSFDDSIHGYLNDTKTLLELFKTSQIRFS
                     CEDLVLENIGTWSAKLLKQQLLSNKLSTSAQSEVEYVLKFPLHSTLDRLEHRRNIEQFK
                     VEGSKVLKSGYCGSHSNEEILALAVDYFHSSQSVYQQELKYFESWVKQCRLDELKFARV
                     MPLIVHFSSAATIFAPELADARMVLSQTCMLITVYDDFFDCPEISREEKENYIALIEKW
                     DNHAEIGFCSKNVEIVFYAVYNTYKQIGEKAALKQNRSIMDQLVEDLVSSAKAMMVEAD
                     WTATKYIPATMEEYMSNAEVSGAFASFVCPPLYFLGLKLSEEDVKSHEYTQLLKLTNVI
                     GRLQNDSQTYRKEILAGKVNSVLLRALTDSGNTSPESIEAAKEIVNRDAESSMVEMRSL
                     VFSEGGPIPRPCKDRFWEMCKIVFYFYSEDDAYRTPKETMSSARAVILDPLRLIPPPSC
                     PETLSS"
     primer_bind     4249..4266
                     /label=F10
     primer_bind     5028..5046
                     /label=F11
     misc_feature    6133..6135
                     /label=3bp_scar9
     misc_feature    6136..6155
                     /label=spacer
     terminator      6156..6202
                     /label=L3S3P41
     misc_feature    6200
                     /label=C to G
                     /note="remove BsaI site"
     misc_feature    6203..6222
                     /label=spacer
     misc_feature    6223..6226
                     /label=4bp_scar6
     terminator      6264..6321
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(6320..6343)
                     /label=oGML0091_r
                     /note="amplify GFP with BbsI(1/2 scar, out) and BsaI(1/6
                     scar, in) for pMBIS derivative"
     rep_origin      complement(6420..8634)
                     /direction=LEFT
                     /label=pSC101 origin
     misc_feature    7095..7100
                     /label=RBS Suffix
     misc_feature    7332
                     /label=C->T for temperature sensitive variant
     misc_feature    7442..7444
                     /label=E93W
     misc_feature    8538
                     /label=fixed point mut (G->C)
     CDS             complement(8730..9590)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(8874)
                     /label=removed BsaI site
     misc_feature    complement(9459)
                     /label=silent point mutation in AmpR cassette
     promoter        complement(9591..9695)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
      121 taaggatgat ttctggaatt cgcggccgct tctagagcgc gaagacttaa tcaaatccgt
      181 agcagtgcac aagcaagtga aatctaatac gactcactat aggggaattg tgagcggata
      241 acaattcccc tctacgtttt caaggtggta gactgtcgcc ggatgtgtat ccgacctgac
      301 gatggcccaa aagggccgaa acagtcctct acaaataatt ttgtttaaga gaataccccg
      361 ctaagaaggt aattatgtcc atgcaaagtt tagataagaa ttttcgacat ttatctcgta
      421 aagaaaagtt acaacaattg gttgataagc aatggttatc agaagaacaa ttcgacattt
      481 tactgaatca tccattaatc gatgaagaag tagccaatag tttaattgaa aatgtcatcg
      541 cgcaaggtgc attacccgtt ggattattac cgaatatcat tgtggacgat aaggcatatg
      601 ttgtacctat gatggtggaa gagccttcag ttgtcgctgc agctagttat ggtgcaaagc
      661 tagtgaatca gactggcgga tttaaaacgg tatcttctga acgtattatg ataggtcaaa
      721 tcgtctttga tggcgttgac gatactgaaa aattatcagc agacattaaa gctttagaaa
      781 agcaaattca taaaattgcg gatgaggcat atccttctat taaagcgcgt ggtggtggtt
      841 accaacgtat agcgattgat acatttcctg agcaacagtt actatcttta aaagtatttg
      901 ttgatacgaa agatgctatg ggcgctaata tgcttaatac gattttagag gccataactg
      961 catttttaaa aaatgaattt ccgcaaagcg acattttaat gagtatttta tccaatcatg
     1021 caacagcgtc cgttgttaaa gttcaaggcg aaattgatgt taaagattta gcaaggggcg
     1081 agagaactgg agaagaggtt gccaaacgaa tggaacgtgc ttctgtattg gcccaagtag
     1141 atattcatcg tgcagcaaca cataataaag gtgttatgaa tggcatacat gctgttgttt
     1201 tagcaacagg aaatgatacg cgtggtgcag aagcaagtgc gcatgcatac gcgagtcgtg
     1261 acggacagta tcgtggtatt gctacatggc gttacgatca agatcgtcaa cgattgattg
     1321 gtacaattga agtgcctatg acattggcaa tcgttggcgg tggtacaaaa gtattaccaa
     1381 ttgctaaagc ttcattagag ctactaaatg tagagtcagc acaagaatta ggtcatgtag
     1441 ttgctgccgt tggtttagcg caaaactttg cagcatgtcg cgcgcttgtg tcagaaggta
     1501 ttcaacaagg tcatatgagt ttacaatata aatcattagc tatcgttgta ggggcaaaag
     1561 gtgatgaaat tgctaaagta gctgaagctt tgaaaaaaga accccgtgca aatacacaag
     1621 cagcggaaca tattttacaa gaaattagac aacaataata acggatgtaa acagagaaac
     1681 gtctaactaa aaaggcctcc caaatcgggg ggcctttttt attgataaca aaagcctaat
     1741 acacctgttc gtgacgatga gacctagccg caatacacag tgtgctaata cgactcacta
     1801 taggggaatt gtgagcggat aacaattccc ctctaaacgc cagccactaa agcggtcaac
     1861 gcatgtgctt tgcgttctga tgagacagtg atgtcgaaac cgcctctaca aataattttg
     1921 tttaagagat tattgattaa ttttaaggag gatttatatg ctggaagttc cggcacagcc
     1981 aacgccagcg cctcgtgaag cagaagcggc agccttattg gcagcgaccg ttgctgatcc
     2041 ttggggctta gtcgcaccta gtgtgtacga cacagctcgt ctggtaagcc ttgccccgtg
     2101 gctggatgga caccgtgaac gcctgggtta cctcgtgaaa gaacagaatc aggatggctc
     2161 atggggcgct ccggacggct atggtctggt ccctaccttg tcggccgttg aagccctgct
     2221 gacggaatta gcgcgcaccg atagtggagc gccgcatcct ccacacgatg atctggctgc
     2281 ggcctgcgcg ggcggacttg gtgcgttaca ggatggcctc ttggctgggc cggttcctga
     2341 tactatcggc gttgagtttg ttgcgccttc attactggcg gatatcaaca cgcgtctggc
     2401 cgcactgaca gaacaagccc ctggcaaact gggcgcatgg tccggtacaa ccctgacgtc
     2461 accagctccg gatctggatg gcgcacttct ggccggcgtt cgtgagatga ctgagcaggc
     2521 gccactgcca gagaaactgt ggcatacctt ggaagccatc acccgcgatg gtacccgtgg
     2581 ggcacgcccc catgaaggtg caccgccaca caatgggtct gttggttgta gtccagcagc
     2641 taccgctgca tggcttggtg cgtcgcctga tcctgcagcg ccaggtgtag catacctgcg
     2701 tgatgttcag gctcgctttg gcggcccggt gccgtccatt acgccgatcg tgtactttga
     2761 gcaggcatgg gtgctgaata gtttagccgc atcgggactg cgctatgaag cgcctgcagc
     2821 attactggac agtctggaag ccggcttgac tgatgaaggc atcgcggcag caccgggcct
     2881 cccttctgac agcgacgata ccgccgcagt attattcgcg ctggcccagc acggtcgtac
     2941 acatcgccca gactccctga tgcacttccg ccgtgatggc tatttttcat gttttggtgt
     3001 cgagcgcacg ccatcaacct caacaaacgc gcatatactg gaagcgttgg gacaccatgt
     3061 tacagtgcgc cccgacgatg ctgggcgtta tggcgccgaa atccgtatga tttctgactg
     3121 gctgctggat aatcaactgc cagatggctc ttggatggat aaatggcacg cgtcacccta
     3181 ttacgccacc gcctgctgtg cccttgctct ggccgaattt ggcggaccga gcgctcgtgc
     3241 tgcagtggat cgtgcggcag cgtgggctct ggaaactcaa cgtgccgatg gcagctgggg
     3301 tcgctggcag ggcacaacag aagaaaccgc atacatggtg cagctcttga tgcgcacccg
     3361 cacgcctggt tcgccaggta ctgtggctag atcggctgcg cgcggctgtg atgccctcct
     3421 ggctcatgat gatccggcct cgtacccggg cctttggcat gataaagaca tttatgcccc
     3481 ggtaacggtg attcgagcgg cacgtttagc cgcgctggct ctgggaggtg cagagtctgc
     3541 cgcgagtggt ggggcgtaat aattatgcct tccgctaatc aactcggtac caaattccag
     3601 aaaagagacg ctttcgagcg tcttttttcg ttttggtccg tggacaagca cagcatagcc
     3661 aagtaatacg actcactata ggggaattgt gagcggataa caattcccct ctaggtccga
     3721 agatacttag ggtgtctcaa ggtgcgtacc ttgactgatg agtccgaaag gacgaaacac
     3781 ccctctacaa ataattttgt ttaagaatac acaagaagct agggagaata acgatgcact
     3841 ctaaggaatt acaggctaaa atacgccgac agctgcagcg tgtggagctc tcaccatctt
     3901 tgtatgatac cgcgtgggtt gcgatggtcc cggagcgcag ttctagtcag gctccctgct
     3961 acccgcagtg tattgaatgg attttgcaga accagcatga cgacgggagc tggggtatta
     4021 actcttcgtc actctctgtt aataaagata tcctgctgtc tacgttggcg tgcgtcgttg
     4081 cgctcaagaa atggaacgca ggtagttatc acattaagag aggcctgaac tttgttggac
     4141 gcaactttag cgtagctatg gacgtccaaa atattgcacc ggttggtttc aatgttacct
     4201 tctctggtct gattaccttg gcctccggga tgggtctgca gctgccggtt tggcagaccg
     4261 atattgatga aattttccac cttcgcaaaa ttgaactgga acgagatagt ggcggaacca
     4321 tttcagcccg caaagcgttt atggcctatg tggcggaggg atttggttcg ttacaggact
     4381 gggatcaggt tatggcatac caacgcaaaa acggctcctt gtttaattca ccgtccacca
     4441 ccgccgctgc ggctatacac accttcaatg atcgtaccct taattatttg gatagcctca
     4501 caaacaaatt tggcggtccg gttccggcaa tgtaccccca gaatatttac tcacagctct
     4561 gtaccgtgga cgcgctggaa cgcacgggta tcagccagaa attcgcgcgc gaaatcagag
     4621 acatcctgga taccacgtac cgctcctggc ttcataatga agaagaagtc atgttagata
     4681 ttcctacctg cgcgatggcc tttcgtctgc tccgcacaca cggctatgat attacgagtg
     4741 atgaaatggc gcacttttct gaacagagct ctttcgatga tagcatccac ggctatctga
     4801 atgacaccaa aactttgctt gaactgttca aaacctcgca gattcgtttt tcttgcgaag
     4861 atcttgtgct ggaaaacatt ggaacatggt cagcaaaact gctgaaacag caactgctgt
     4921 caaacaaact gagcacatca gcccagagtg aggtagaata cgtactcaag tttccgttac
     4981 atagtaccct ggaccgtctg gaacaccgtc gcaacattga acagtttaaa gttgagggct
     5041 ccaaagtatt aaaatcaggt tattgcggtt ctcattcgaa cgaggaaatt ctggcactgg
     5101 ctgtagatta ttttcattca tctcaaagcg tataccagca ggaactgaaa tattttgaaa
     5161 gctgggtcaa acagtgtcgt ttggatgagc tgaaattcgc gcgcgtgatg ccgctgatcg
     5221 ttcatttcag cagtgcggcg acaatcttcg cgccggaatt ggcggatgca cgaatggttc
     5281 tgtcgcagac ctgtatgtta attacggtgt acgatgattt ctttgattgc ccggagatca
     5341 gtcgcgaaga gaaggagaac tatatagcgt taattgagaa atgggacaat cacgcggaaa
     5401 tcgggttttg cagcaaaaat gtagaaatcg ttttctatgc ggtatacaac acgtacaaac
     5461 agattggtga aaaagcagcg ctgaaacaga accggtccat tatggatcaa ttagtggaag
     5521 atctggtgag cagtgcgaaa gcgatgatgg ttgaagcaga ttggacagcc acgaaatata
     5581 tcccagcgac aatggaagag tatatgtcaa acgcagaggt aagtggtgcg tttgcctcgt
     5641 ttgtatgccc acctctgtac tttctgggtc tgaaactgtc cgaagaggac gttaaaagtc
     5701 atgagtatac gcagcttctg aagttgacta acgtgattgg acgcttacag aacgactctc
     5761 agacctatcg taaagaaatc ctggcaggta aagtgaatag cgtgcttctg cgcgcgctga
     5821 ccgattccgg gaatacttcg ccggaaagta ttgaagcagc aaaagaaatt gtcaatcggg
     5881 acgcggaatc tagcatggta gaaatgcgct cgcttgtgtt ttcggagggc ggcccgattc
     5941 cgcggccttg taaagacaga ttctgggaaa tgtgcaaaat tgttttctac ttctatagcg
     6001 aggacgacgc ctatcgcact cctaaagaaa cgatgtcaag cgcgcgtgca gttattcttg
     6061 acccgctgcg actgataccg cccccgtcct gtccggaaac cttatctagt taataagcct
     6121 cgttgctctt cataaatcat ccctatgacc ccttcaaaaa aaaaaaacac cctaacgggt
     6181 gttttttttt ttttggtctg cctctcgctg acggtcacaa tgcgcttacg aaagtcttcc
     6241 tgtactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc accctgccct
     6301 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
     6361 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatgaca
     6421 gtaagacggg taagcctgtt gatgataccg ctgccttact gggtgcatta gccagtctga
     6481 atgacctgtc acgggataat ccgaagtggt cagactggaa aatcagaggg caggaactgc
     6541 tgaacagcaa aaagtcagat agcaccacat agcagacccg ccataaaacg ccctgagaag
     6601 cccgtgacgg gcttttcttg tattatgggt agtttccttg catgaatcca taaaaggcgc
     6661 ctgtagtgcc atttaccccc attcactgcc agagccgtga gcgcagcgaa ctgaatgtca
     6721 cgaaaaagac agcgactcag gtgcctgatg gtcggagaca aaaggaatat tcagcgattt
     6781 gcccgagctt gcgagggtgc tacttaagcc tttagggttt taaggtctgt tttgtagagg
     6841 agcaaacagc gtttgcgaca tccttttgta atactgcgga actgactaaa gtagtgagtt
     6901 atacacaggg ctgggatcta ttctttttat ctttttttat tctttcttta ttctataaat
     6961 tataaccact tgaatataaa caaaaaaaac acacaaaggt ctagcggaat ttacagaggg
     7021 tctagcagaa tttacaagtt ttccagcaaa ggtctagcag aatttacaga tacccacaac
     7081 tcaaaggaaa aggactagta attatcattg actagcccat ctcaattggt atagtgatta
     7141 aaatcaccta gaccaattga gatgtatgtc tgaattagtt gttttcaaag caaatgaact
     7201 agcgattagt cgctatgact taacggagca tgaaaccaag ctaattttat gctgtgtggc
     7261 actactcaac cccacgattg aaaaccctac aaggaaagaa cggacggtat cgttcactta
     7321 taaccaatac gctcagatga tgaacatcag tagggaaaat gcttatggtg tattagctaa
     7381 agcaaccaga gagctgatga cgagaactgt ggaaatcagg aatcctttgg ttaaaggctt
     7441 ttggattttc cagtggacaa actatgccaa gttctcaagc gaaaaattag aattagtttt
     7501 tagtgaagag atattgcctt atcttttcca gttaaaaaaa ttcataaaat ataatctgga
     7561 acatgttaag tcttttgaaa acaaatactc tatgaggatt tatgagtggt tattaaaaga
     7621 actaacacaa aagaaaactc acaaggcaaa tatagagatt agccttgatg aatttaagtt
     7681 catgttaatg cttgaaaata actaccatga gtttaaaagg cttaaccaat gggttttgaa
     7741 accaataagt aaagatttaa acacttacag caatatgaaa ttggtggttg ataagcgagg
     7801 ccgcccgact gatacgttga ttttccaagt tgaactagat agacaaatgg atctcgtaac
     7861 cgaacttgag aacaaccaga taaaaatgaa tggtgacaaa ataccaacaa ccattacatc
     7921 agattcctac ctacataacg gactaagaaa aacactacac gatgctttaa ctgcaaaaat
     7981 tcagctcacc agttttgagg caaaattttt gagtgacatg caaagtaagt atgatctcaa
     8041 tggttcgttc tcatggctca cgcaaaaaca acgaaccaca ctagagaaca tactggctaa
     8101 atacggaagg atctgaggtt cttatggctc ttgtatctat cagtgaagca tcaagactaa
     8161 caaacaaaag tagaacaact gttcaccgtt acatatcaaa gggaaaactg tccatatgca
     8221 cagatgaaaa cggtgtaaaa aagatagata catcagagct tttacgagtt tttggtgcat
     8281 tcaaagctgt tcaccatgaa cagatcgaca atgtaacaga tgaacagcat gtaacaccta
     8341 atagaacagg tgaaaccagt aaaacaaagc aactagaaca tgaaattgaa cacctgagac
     8401 aacttgttac agctcaacag tcacacatag acagcctgaa acaggcgatg ctgcttatcg
     8461 aatcaaagct gccgacaaca cgggagccag tgacgcctcc cgtggggaaa aaatcatggc
     8521 aattctggaa gaaatagcgc tttcagccgg caaaccggct gaagccggat ctgcgattct
     8581 gataacaaac tagcaacacc agaacagccc gtttgcgggc agcaaaaccc gtaccgatta
     8641 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8701 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8761 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8821 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc
     8881 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8941 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9001 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9061 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9121 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9181 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9241 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9301 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9361 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9421 ctctcaagga tcttaccgct gttgagatcc agttcgatat aacccactcg tgcacccaac
     9481 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9541 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9601 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9661 tgtatttaga aaaataaaca aataggggtt ccgcg
//
