LOCUS       ABE-RA3.1               8220 bp ds-DNA     circular     23-AUG-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          170..194
                     /label="WT1399"
                     /note="sequence: TACAAAGAGATTTTCTTCGACCAGA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     381..401
                     /label="HRSeq07"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(381..401)
                     /label="HRSeq08"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          670..694
                     /label="WT1400"
                     /note="sequence: GCTGTACGAGTACTTCACCGTGTAT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1170..1194
                     /label="WT1401"
                     /note="sequence: TGGATTTCCTGAAGTCCGACGGCTT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     1297..1316
                     /label="HRSeq09"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(1297..1316)
                     /label="HRSeq10"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     Mutation        1649..1651
                     /label="H840"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     1670..1694
                     /label="KZ683"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1670..1694
                     /label="WT1402"
                     /note="sequence: CTGAAGGACGACTCCATCGACAACA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     2170..2194
                     /label="KZ684"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          2170..2194
                     /label="WT1403"
                     /note="sequence: CAAGGTGTACGACGTGCGGAAGATG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     2344..2362
                     /label="HRSeq11"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(2344..2362)
                     /label="HRSeq12"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2670..2694
                     /label="WT1404"
                     /note="sequence: ACTTTCTGGAAGCCAAGGGCTACAA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          3170..3194
                     /label="WT1405"
                     /note="sequence: CTGATCCACCAGAGCATCACCGGCC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(3199..3220)
                     /label="WT936"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     3213..3231
                     /label="WT935"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3236..3247
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3236..3247
                     /label="Translation 3236-3247"
                     /translation="SGGS"
     misc_feature    3248..3277
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     sig_peptide     3248..3298
                     /label="BP_NLS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     3266..3322
                     /label="WT920"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3278..3298
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(3347..3364)
                     /label="Eton BGH Reverse"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     Terminator      3350..3577
                     /label="bGH_PA term"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     3376..3394
                     /label="436_bGH_QPCRF2"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3408..3432)
                     /label="WT1406"
                     /note="sequence: AGTGGGAGTGGCACCTTCCAGGGTC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(3499..3523)
                     /label="ACK64"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(3513..3532)
                     /label="438_bGH_QPCRR2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(3518..3536)
                     /label="439_bGH_QPCRR3"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(3538..3557)
                     /label="437_bGH_QPCRR1"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(3648..3664)
                     /label="Genewiz M13R"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(3661..3684)
                     /label="M13-R (-46)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     Promoter        complement(3697..3726)
                     /label="lac prom"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     3741..3765
                     /label="ACK21"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(3741..3765)
                     /label="ACK32"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(3879..3896)
                     /label="QB0039 L4440"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(3931..3950)
                     /label="VR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     complement(4020..4041)
                     /label="11_pFYF_Seq_Rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(4035..4654)
                     /label="pBR322 origin"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     4050..4074
                     /label="ACK10"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(4111..4130)
                     /label="BR322-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4244..4245
                     /label="A"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    4246..4246
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     4273..4291
                     /label="QB0766 RepO-F"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     4370..4389
                     /label="833_BR322-rev1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(4398..4417)
                     /label="QB0767 RepO-R"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4491..4491
                     /label="G"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(4551..4570)
                     /label="832_BR322-fwd1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     4551..4575
                     /label="ACK11"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     Marker          complement(4809..5669)
                     /label="amp marker"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(4928..4960)
                     /label="WT958"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4928..4960)
                     /label="WT958"
                     /note="sequence: agcgtggtagccgcggtatcattgcagcactgg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     modified_base   complement(4949..4954)
                     /label="modified BsaI site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     4953..4970
                     /label="WT957"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          4953..4970
                     /label="WT957"
                     /note="sequence: accacgctcaccggctcc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     5050..5074
                     /label="ACK12"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     5215..5234
                     /label="ACK563"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     5550..5574
                     /label="ACK13"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     5646..5669
                     /label="WT023"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     Promoter        complement(5711..5739)
                     /label="amp prom"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     5783..5800
                     /label="QB0090 pQE promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     complement(5853..5872)
                     /label="QB0092 pRS-marker"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     6053..6077
                     /label="ACK14"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     6060..6103
                     /label="387_pAAV_fwd "
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     promoter        6060..6648
                     /label="CMV_Full"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(6110..6129)
                     /label="QB1110 pSicoR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    6249..6249
                     /label="C"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          6511..6535
                     /label="WT1395-seq"
                     /note="sequence: GGGAGTTTGTTTTGGCACCAAAATC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     6526..6545
                     /label="LCv2_seq1"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     6544..6564
                     /label="seq CMV forward"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     6553..6577
                     /label="ACK15"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     Promoter        6567..6648
                     /label="CMV prom"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     6578..6598
                     /label="Eton CMV-Forward"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Promoter        6669..6687
                     /label="T7 prom"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(6686..6734)
                     /label="WT918"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     RBS             6695..6700
                     /label="mRBS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    6698..6700
                     /label="USER1"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     sig_peptide     6704..6757
                     /label="BP_NLS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             6704..6757
                     /label="Translation 6704-6757"
                     /translation="KRTADGSEFESPKKKRKV"
     primer          complement(6733..6756)
                     /label="WT1385"
                     /note="sequence: actttccgcttcttctttggtgac"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(6733..6773)
                     /label="WT1417"
                     /note="sequence: aggtagggtggtctcTgactttccgcttcttctttggtgac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    6754..6757
                     /label="ligase site"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             6758..7258
                     /label="TadA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             6758..7258
                     /label="Translation 6758-7258"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDERDVPVGAVLVHNNRVIGEGWNKAIGHHDPTAHAEIMALRQGGLVMQNYRLFDATLYVTLEPCVMCAGAMIHSRIGRVVFGARGARTGAAGSLMDVLRHPGIKHRVEITEGILADECAALLSDFFRMRRQEIKAQKNAQSSTD"
     primer          complement(6821..6842)
                     /label="YX242"
                     /note="sequence: GATTGGTCTCAggacttctctttcatcccatgc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6836..6838
                     /label="E27D"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          6838..6854
                     /label="YX241"
                     /note="sequence: GATTGGTCTCAgtccccgtgggcgccg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6896..6898
                     /label="R47K"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    6899..6901
                     /label="P48A"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(6905..6923)
                     /label="YX256"
                     /note="sequence: GATTGGTCTCGgtagggtcgtggcggcc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6908..6910
                     /label="R51H"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          6916..6934
                     /label="YX243"
                     /note="sequence: GATTGGTCTCCctaccgcacacgcagag"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6978..6995
                     /label="illumiF YX130"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    6983..6985
                     /label="I76F"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(6994..7014)
                     /label="YX246"
                     /note="sequence: GATTGGTCTCGgctccagtgtcacatacagg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          7009..7027
                     /label="YX245"
                     /note="sequence: GATTGGTCTCGgagccatgcgtgatgtgc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7012..7029)
                     /label="illumiR YX240"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    7079..7081
                     /label="D108G"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    7085..7087
                     /label="K110R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(7087..7101)
                     /label="YX248"
                     /note="sequence: GATTGGTCTCCctgctgcgccggtc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          7091..7113
                     /label="YX247"
                     /note="sequence: GATTGGTCTCAgcaggctccctgatgga"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7100..7118)
                     /label="illumiR YX132"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    7121..7123
                     /label="H122R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    7133..7135
                     /label="M126I"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7136..7138
                     /label="N127K"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(7167..7182)
                     /label="YX250"
                     /note="sequence: GATTGGTCTCGcgcactcgtctgcca"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          7177..7192
                     /label="YX249"
                     /note="sequence: GATTGGTCTCGtgcgccgccctgctg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7238..7240
                     /label="K161N"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(7240..7258)
                     /label="illumiR YX131"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          7249..7278
                     /label="WT1416"
                     /note="sequence: ataggtctcatctggaggatctagcggagg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    7259..7262
                     /label="ligase site"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             7259..7354
                     /label="Genscript BE4 linker"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             7259..7354
                     /label="Translation 7259-7354"
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     primer          7264..7282
                     /label="WT1383"
                     /note="sequence: aggatctagcggaggatcc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(7325..7354)
                     /label="HY102"
                     /note="sequence: tgtcggggtctcttgctgctgccgccgctgctgccgccactgctctc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          7354..7390
                     /label="WT922"
                     /note="sequence: AGTTCGAGTCACCAAAGAAGAAGCGGAAAGTCGACAAGAAGTACAGCATCGGCCTGGACATCGGCACC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             7355..3235
                     /label="nCas9-D10A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             7355..3235
                     /label="Translation 7355-3235"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     Mutation        7379..7381
                     /label="D10A"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    7380..7380
                     /label="A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          7390..7414
                     /label="WT1397"
                     /note="sequence: CAACTCTGTGGGCTGGGCCGTGATC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(7494..7518)
                     /label="WT1396"
                     /note="sequence: CCGCTGTCGAACAGCAGGGCTCCGA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     7756..7777
                     /label="HRSeq05"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(7756..7777)
                     /label="HRSeq06"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          7890..7914
                     /label="WT1398"
                     /note="sequence: ACGTGGACAAGCTGTTCATCCAGCT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac
       61 cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca
      121 ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat
      181 tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga
      241 agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct
      301 cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat
      361 cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta
      421 cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta
      481 ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga
      541 ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag
      601 cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa
      661 gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt
      721 gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga
      781 cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa
      841 gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc
      901 cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga
      961 ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga
     1021 gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca
     1081 gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat
     1141 ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa
     1201 cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa
     1261 agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag
     1321 ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt
     1381 gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac
     1441 ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga
     1501 gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa
     1561 gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat
     1621 caaccggctg tccgactacg atgtggacca tatcgtgcct cagagctttc tgaaggacga
     1681 ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt
     1741 gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa
     1801 gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga
     1861 actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca
     1921 cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat
     1981 ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt
     2041 ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa
     2101 cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta
     2161 cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg
     2221 caaggctacc gccaagtact tcttctacag caacatcatg aactttttca agaccgagat
     2281 taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg
     2341 ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc
     2401 ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat
     2461 cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa
     2521 gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga
     2581 aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga
     2641 aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt
     2701 gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg
     2761 gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc
     2821 caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga
     2881 ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat
     2941 cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt
     3001 gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat
     3061 ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac
     3121 catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca
     3181 gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag gtgactctgg
     3241 cggctcaaaa agaaccgccg acggcagcga attcgagccc aagaagaaga ggaaagtcta
     3301 accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct
     3361 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     3421 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     3481 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     3541 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     3601 tggggctcga taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     3661 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     3721 tgtaaagcct agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     3781 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     3841 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     3901 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     3961 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4021 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4081 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4141 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4201 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4261 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4321 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4381 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4441 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     4501 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4561 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4621 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4681 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4741 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4801 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4861 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4921 tctggcccca gtgctgcaat gataccgcgg ctaccacgct caccggctcc agatttatca
     4981 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     5041 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     5101 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     5161 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     5221 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     5281 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     5341 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     5401 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     5461 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     5521 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     5581 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     5641 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     5701 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5761 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     5821 cgatctcccg atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta
     5881 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     5941 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     6001 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     6061 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     6121 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     6181 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     6241 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     6301 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     6361 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     6421 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     6481 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     6541 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     6601 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagagatcc
     6661 gcggccgcta atacgactca ctatagggag agccgccacc atgaaacgga cagccgacgg
     6721 aagcgagttc gagtcaccaa agaagaagcg gaaagtcatg tctgaagtcg agtttagcca
     6781 cgagtattgg atgaggcacg cactgaccct ggcaaagcga gcatgggatg aaagagaTgt
     6841 ccccgtgggc gccgtgctgg tgcacaacaa tagagtgatc ggagagggat ggaacaAgGc
     6901 aatcggccAc cacgacccta ccgcacacgc agagatcatg gcactgaggc agggaggcct
     6961 ggtcatgcag aattaccgcc tgTtcgatgc caccctgtat gtgacactgg agccatgcgt
     7021 gatgtgcgca ggagcaatga tccacagcag gatcggaaga gtggtgttcg gagcacgggG
     7081 cgccaGgacc ggcgcagcag gctccctgat ggatgtgctg cGccaccccg gcatAaaAca
     7141 ccgggtggag atcacagagg gaatcctggc agacgagtgc gccgccctgc tgagcgattt
     7201 ctttagaatg cggagacagg agatcaaggc ccagaagaaC gcacagagct ccaccgactc
     7261 tggaggatct agcggaggat cctctggcag cgagacacca ggaacaagcg agtcagcaac
     7321 accagagagc agtggcggca gcagcggcgg cagcgacaag aagtacagca tcggcctggc
     7381 catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa
     7441 gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc
     7501 cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag
     7561 aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat
     7621 ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga
     7681 taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga
     7741 gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga
     7801 cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat
     7861 cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca
     7921 gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc
     7981 catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc
     8041 cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc
     8101 caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac
     8161 ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt
//