LOCUS       pYX075b_052621_pET28a_H 6961 bp ds-DNA     circular     30-JAN-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             107..475
                     /label="mobilization protein associated with ROP"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          650..667
                     /label="HY007-Seq"
                     /note="sequence: gacaggccgatcatcgtc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(884..1966)
                     /label="lacI"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1241..1259
                     /label="HY008-Seq"
                     /note="sequence: catcgcagtgggaacgatg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        complement(1967..2044)
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          2339..2361
                     /label="HY009-Seq"
                     /note="sequence: cgatcccgcgaaattaatacgac"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        2353..2371
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    2372..2396
                     /label="lac operator"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     protein_bind    2374..2394
                     /label="lac repressor binding site"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     RBS             2411..2433
                     /label="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2440..2442
                     /label="start codon"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             2440..4164
                     /label="Translation 2440-4164"
                     /translation="MGSSHHHHHHSSGLVPRGSHMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTSGGSSGSENLYFQGSGSSGGSMSEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFGVRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQSSIN"
     CDS             2452..2469
                     /label="6xHis"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             2479..2496
                     /label="thrombin site"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             2503..3600
                     /label="MBP"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             2503..3600
                     /label="Translation 2503-3600"
                     /translation="KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT"
     primer          complement(2531..2558)
                     /label="YX147"
                     /note="sequence: GATTGGTCTCACCGTTATAGCCTTTATCGCCGTTAATCC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2554..2584
                     /label="YX146"
                     /note="sequence: GATTGGTCTCAACGGaCTCGCTGAAGTCGGTAAGAAATTCG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2555..2558
                     /label="ligase site"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2557..2559
                     /label="codon GGT change to GGa "
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3581..3634)
                     /label="YX373"
                     /note="sequence: AGTACAGGTTTTCggatccgctagatcctccagaAGTCTGCGCGTCTTTCAGGG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3601..3604
                     /label="ligase site"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          3603..3621
                     /label="WT1383"
                     /note="sequence: aggatctagcggaggatcc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             3622..3642
                     /label="TEV site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3626..3634
                     /label="user junction"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          3626..3685
                     /label="YX372"
                     /note="sequence: ACCTGTACTTCCAGGGAagcggcagcagcggcggcagcatgtctgaggtggagttttccc"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3642..3642
                     /label="ligase site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             3664..4164
                     /label="TadA8e"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             3664..4164
                     /label="Translation 3664-4164"
                     /translation="MSEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFGVRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQSSIN"
     CDS             3667..4164
                     /label="TadA*"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     3803..3827
                     /label="KZ919"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(3972..4012)
                     /label="WT1548"
                     /note="sequence: GATTGGTCTCgggagcctgcggcgccgCGttttgAgttcctcacgccaaac"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3988..3988
                     /label="A109S"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3994..3995
                     /label="T111R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          4004..4045
                     /label="WT1549"
                     /note="sequence: GATTGGTCTCgctccctgatgAacgtgctgAactaccccggcatgaatc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    4018..4018
                     /label="D119N"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4027..4028
                     /label="H122N"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(4089..4145)
                     /label="WT1550"
                     /note="sequence: ttcttctgagcattgaacacctgtctaggcatccgaTagaaatCgcacagcagggcg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4102..4102
                     /label="Y147D"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4109..4109
                     /label="F149Y"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4160..4160
                     /label="T166I"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    4162..4162
                     /label="D167N"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4165..4168
                     /label="BsaI ligase site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          4167..4193
                     /label="WT1391"
                     /note="sequence: actcgagcaTcaccaccaccaccactg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             4174..4191
                     /label="6xHis"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     terminator      4258..4305
                     /label="T7 terminator"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(4285..4305)
                     /label="WT1335"
                     /note="sequence: AAAACGTCTCtgggtaCAAAAAACCCCTCAAGACCCG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      4342..4797
                     /label="f1 ori"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(4890..5705)
                     /label="KanR"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      5827..6415
                     /label="ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    6601..6743
                     /label="bom"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             complement(6845..75)
                     /label="rop"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
       61 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
      121 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
      181 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat
      241 cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca
      301 gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc
      361 cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt
      421 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
      481 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg
      541 tggggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc
      601 agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat
      661 catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac
      721 ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc
      781 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc
      841 cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca
      901 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
      961 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
     1021 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
     1081 gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg
     1141 tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg
     1201 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
     1261 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
     1321 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
     1381 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
     1441 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
     1501 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
     1561 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
     1621 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
     1681 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
     1741 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
     1801 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
     1861 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
     1921 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
     1981 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
     2041 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
     2101 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
     2161 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
     2221 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
     2281 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg
     2341 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc
     2401 tagaaataat tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat
     2461 catcatcaca gcagcggcct ggtgccgcgc ggcagccata tgAAAATCGA AGAAGGTAAA
     2521 CTGGTAATCT GGATTAACGG CGATAAAGGC TATAACGGaC TCGCTGAAGT CGGTAAGAAA
     2581 TTCGAGAAAG ATACCGGAAT TAAAGTCACC GTTGAGCATC CGGATAAACT GGAAGAGAAA
     2641 TTCCCACAGG TTGCGGCAAC TGGCGATGGC CCTGACATTA TCTTCTGGGC ACACGACCGC
     2701 TTTGGTGGCT ACGCTCAATC TGGCCTGTTG GCTGAAATCA CCCCGGACAA AGCGTTCCAG
     2761 GACAAGCTGT ATCCGTTTAC CTGGGATGCC GTACGTTACA ACGGCAAGCT GATTGCTTAC
     2821 CCGATCGCTG TTGAAGCGTT ATCGCTGATT TATAACAAAG ATCTGCTGCC GAACCCGCCA
     2881 AAAACCTGGG AAGAGATCCC GGCGCTGGAT AAAGAACTGA AAGCGAAAGG TAAGAGCGCG
     2941 CTGATGTTCA ACCTGCAAGA ACCGTACTTC ACCTGGCCGC TGATTGCTGC TGACGGGGGT
     3001 TATGCGTTCA AGTATGAAAA CGGCAAGTAC GACATTAAAG ACGTGGGCGT GGATAACGCT
     3061 GGCGCGAAAG CGGGTCTGAC CTTCCTGGTT GACCTGATTA AAAACAAACA CATGAATGCA
     3121 GACACCGATT ACTCCATCGC AGAAGCTGCC TTTAATAAAG GCGAAACAGC GATGACCATC
     3181 AACGGCCCGT GGGCATGGTC CAACATCGAC ACCAGCAAAG TGAATTATGG TGTAACGGTA
     3241 CTGCCGACCT TCAAGGGTCA ACCATCCAAA CCGTTCGTTG GCGTGCTGAG CGCAGGTATT
     3301 AACGCCGCCA GTCCGAACAA AGAGCTGGCA AAAGAGTTCC TCGAAAACTA TCTGCTGACT
     3361 GATGAAGGTC TGGAAGCGGT TAATAAAGAC AAACCGCTGG GTGCCGTAGC GCTGAAGTCT
     3421 TACGAGGAAG AGTTGGCGAA AGATCCACGT ATTGCCGCCA CTATGGAAAA CGCCCAGAAA
     3481 GGTGAAATCA TGCCGAACAT CCCGCAGATG TCCGCTTTCT GGTATGCCGT GCGTACTGCG
     3541 GTGATCAACG CCGCCAGCGG TCGTCAGACT GTCGATGAAG CCCTGAAAGA CGCGCAGACT
     3601 tctggaggat ctagcggatc cGAAAACCTG TACTTCCAGG GAagcggcag cagcggcggc
     3661 agcatgtctg aggtggagtt ttcccacgag tactggatga gacatgccct gaccctggcc
     3721 aagagggcac gcgatgagag ggaggtgcct gtgggagccg tgctggtgct gaacaataga
     3781 gtgatcggcg agggctggaa cagagccatc ggcctgcacg acccaacagc ccatgccgaa
     3841 attatggccc tgagacaggg cggcctggtc atgcagaact acagactgat tgacgccacc
     3901 ctgtacgtga cattcgagcc ttgcgtgatg tgcgccggcg ccatgatcca ctctaggatc
     3961 ggccgcgtgg tgtttggcgt gaggaacTca aaaCGcggcg ccgcaggctc cctgatgAac
     4021 gtgctgAact accccggcat gaatcaccgc gtcgaaatta ccgagggaat cctggcagat
     4081 gaatgtgccg ccctgctgtg cGatttctAt cggatgccta gacaggtgtt caatgctcag
     4141 aagaaggccc agagctccaT cAactaactc gagcaTcacc accaccacca ctgagatccg
     4201 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     4261 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
     4321 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4381 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4441 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4501 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4561 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4621 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4681 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4741 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag
     4801 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4861 caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac
     4921 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     4981 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     5041 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     5101 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
     5161 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     5221 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     5281 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     5341 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     5401 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     5461 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     5521 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     5581 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     5641 tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg
     5701 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa
     5761 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5821 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5881 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5941 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     6001 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6061 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6121 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6181 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6241 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6301 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6361 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6421 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6481 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6541 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     6601 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc
     6661 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     6721 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     6781 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     6841 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat
     6901 cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga
     6961 g
//