LOCUS       Supplementary_Fi       15782 bp DNA     circular SYN 20-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2023 from SnapGene Viewer 7.0.2
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..15782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    136..597
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     CDS             639..722
                     /codon_start=1
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.9"
                     /note="/other=CDS"
                     /translation="DHNQPYHICRGFTCFKKPPTPPPEPET"
     polyA_signal    745..879
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             893..976
                     /codon_start=1
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.9"
                     /note="/other=CDS"
                     /translation="DHNQPYHICRGFTCFKKPPTPPPEPET"
     polyA_signal    999..1133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             1147..1230
                     /codon_start=1
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.9"
                     /note="/other=CDS"
                     /translation="DHNQPYHICRGFTCFKKPPTPPPEPET"
     polyA_signal    1253..1387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1416..1449)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             1507..1884
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     CDS             1903..2610
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDI
                     ALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVA
                     KYSNLGGGMDELYK"
     CDS             2620..2676
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2674..2811
                     /codon_start=1
                     /label=Elk1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=95.7"
                     /note="/match_length=10.7"
                     /note="/other=CDS"
                     /translation="PMKGRKPRDLELPLSPSLLGGQGPERTPGSGTSSGLQAPGPALSP
                     S"
     exon            2710..2721
                     /label=PXSP/PXTP Motif
                     /note="Serine is S325 in mouse"
     misc_feature    2743..2757
                     /label=PXSP/PXTP Motif
                     /note="T337 in mouse. Motif has 1 extra aa vs consensus. 
                     phosphorylated by Erk1/2 in vitro according to 
                     PhosphoSite.org"
     exon            2794..2808
                     /product="S is actually a T in mouse/rat/human. T354. human
                     protein phosphorylated by ERK2 in vitro according to 
                     PhosphoSite.org "
                     /label=PXSP/PXTP Motif
                     /note="S is a T in human/mouse/rat. Human protein 
                     phosphorylated by ERK2 in vitro according to 
                     PhosphoSite.org. T354 in mouse."
     misc_feature    2812..2853
                     /label=Phosphorylation-Inhibited NLS
     misc_feature    2833..2844
                     /label=PXSP/PXTP Motif
     misc_feature    2854..2865
                     /label=PXSP/PXTP Motif
     misc_feature    2860..2898
                     /label=Phosphorylation-Enhanced NES
     CDS             2929..3612
                     /codon_start=1
                     /label=mClover3
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=95.4"
                     /note="/other=CDS"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAA"
     misc_feature    3621..4209
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4092..4103)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    4210..4229
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.5"
                     /note="/other=misc_feature"
     primer_bind     complement(4212..4228)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4236..4347
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4448..8792
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     misc_feature    complement(8805..8837)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=30.6"
                     /note="/other=misc_feature"
     promoter        8842..9341
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             9368..9940
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     CDS             9943..10015
                     /codon_start=1
                     /label=small t antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=13.9"
                     /note="/other=CDS"
                     /translation="SL*RNLTSVV*HNWTNYLQRFKAL"
     intron          10017..10082
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    10156..10380
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        10384..10405
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
     promoter        complement(10442..10460)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10481..10497)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10505..10521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10529..10559)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10574..10595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(10608..10700)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      complement(10883..11471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11642..12502)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12503..12607)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12634..13089
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(13093..13248)
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
                     /translation="SLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQ
                     LRSLNGE"
     primer_bind     13230..13246
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        13256..13274
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    13293..14374
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    14376..14479
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     protein_bind    complement(14529..14562)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        14592..15091
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             join(15105..15782,1..126)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
       61 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
      121 ttctgagggg atccgctgta agtctgcaga aattgatgat ctattaaaca ataaagatgt
      181 ccactaaaat ggaagttttt cctgtcatac tttgttaaga agggtgagaa cagagtacct
      241 acattttgaa tggaaggatt ggagctacgg gggtgggggt ggggtgggat tagataaatg
      301 cctgctcttt actgaaggct ctttactatt gctttatgat aatgtttcat agttggatat
      361 cataatttaa acaagcaaaa ccaaattaag ggccagctca ttcctcccac tcatgatcta
      421 tagatctata gatctctcgt gggatcattg tttttctctt gattcccact ttgtggttct
      481 aagtactgtg gtttccaaat gtgtcagttt catagcctga agaacgagat cagcagcctc
      541 tgttccacat acacttcatt ctcagtattg ttttgccaag ttctaattcc atcagaagct
      601 tgcagatctg cgactctaga ggatctgcga ctctagagga tcataatcag ccataccaca
      661 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      721 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      781 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      841 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gcgactctag aggatcataa
      901 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
      961 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     1021 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     1081 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgcgact
     1141 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     1201 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     1261 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     1321 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1381 ctggatcccc atcaagctga tccggaaccc ttaatataac ttcgtataat gtatgctata
     1441 cgaagttatt aggtccctcg acctgcagcc caagctagca gacccaagct ggctagcacc
     1501 gccaccatgc ctgaaccctc taagtctgct ccagccccta aaaagggttc taagaaggct
     1561 atcactaagg cgcagaagaa ggatggtaag aagcgtaagc gcagccgcaa ggagagctat
     1621 tctatctatg tgtacaaggt tctgaagcag gtccaccccg acaccggcat ctcatccaag
     1681 gccatgggga tcatgaactc cttcgtcaac gacatcttcg agcgcatcgc gggcgaggct
     1741 tctcgcctgg ctcactacaa taagcgctcg accatcacct ccagggagat tcagacggct
     1801 gtgcgcctgc tgctgcctgg ggagctggct aagcatgctg tgtccgaggg cactaaggca
     1861 gttaccaagt acactagctc taaggatcca ccggtcgcca ccgtgtctaa gggcgaagag
     1921 ctgatcaagg aaaatatgcg tatgaaggtg gtcatggaag gttcggtcaa cggccaccaa
     1981 ttcaaatgca caggtgaagg agaaggcaga ccgtacgagg gagtgcaaac catgaggatc
     2041 aaagtcatcg agggaggacc cctgccattt gcctttgaca ttcttgccac gtcgttcatg
     2101 tatggcagcc gtacctttat caagtacccg gccgacatcc ctgatttctt taaacagtcc
     2161 tttcctgagg gttttacttg ggaaagagtt acgagatacg aagatggtgg agtcgtcacc
     2221 gtcacgcagg acaccagcct tgaggatggc gagctcgtct acaacgtcaa ggtcagaggg
     2281 gtaaactttc cctccaatgg tcccgtgatg cagaagaaga ccaagggttg ggagcctaat
     2341 acagagatga tgtatccagc agatggtggt ctgagaggat acactgacat cgcactgaaa
     2401 gttgatggtg gtggccatct gcactgcaac ttcgtgacaa cttacaggtc aaaaaagacc
     2461 gtcgggaaca tcaagatgcc cggtgtccat gccgttgatc accgcctgga aaggatcgag
     2521 gagagtgaca atgaaaccta cgtagtgcaa agagaagtgg cagttgccaa atacagcaac
     2581 cttggtggtg gcatggacga gctgtacaag ggaagcggag ctactaactt cagcctgctg
     2641 aagcaggctg gagacgtgga ggagaaccct ggacctatga agggccgaaa gcctcgggac
     2701 ctggaacttc cacttagccc aagcctgctg ggtggccagg gacctgaacg gactccagga
     2761 tcaggaacaa gctctggtct tcaggcaccg gggccagcgc tttcgcccag caagcgtagc
     2821 ggtctcgagg acccagcgac gccatcgaag aagccacgta ccccatcggt gtcgtcaagg
     2881 ctggagcgcc tgaccctcca gtccagcttt caattcccat ccactagtat ggtgagcaag
     2941 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     3001 ggccacaagt tcagcgtccg cggcgagggc gagggcgatg ccaccaacgg caagctgacc
     3061 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     3121 ttcggctacg gcgtggcctg cttcagccgc taccccgacc acatgaagca gcacgacttc
     3181 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatctcttt caaggacgac
     3241 ggtacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     3301 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     3361 aacttcaaca gccactacgt ctatatcacg gccgacaagc agaagaactg catcaaggct
     3421 aacttcaaga tccgccacaa cgttgaggac ggcagcgtgc agctcgccga ccactaccag
     3481 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagccat
     3541 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3601 gtgaccgccg cctagtcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     3661 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     3721 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     3781 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     3841 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     3901 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     3961 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4021 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4081 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4141 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4201 cctccccgca tcgataccgt cgacctcgac ctcgactgtg ccttctagtt gccagccatc
     4261 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     4321 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     4381 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacagctagc gtttaaactt
     4441 aagctttcta gaagatgggc gggagtcttc tgggcaggct taaaggctaa cctggtgtgt
     4501 gggcgttgtc ctgcagggga attgaacagg tgtaaaattg gagggacaag acttcccaca
     4561 gattttcggt tttgtcggga agttttttaa taggggcaaa taaggaaaat gggaggatag
     4621 gtagtcatct ggggttttat gcagcaaaac tacaggttat tattgcttgt gatccgcctc
     4681 ggagtatttt ccatcgaggt agattaaaga catgctcacc cgagttttat actctcctgc
     4741 ttgagatcct tactacagta tgaaattaca gtgtcgcgag ttagactatg taagcagaat
     4801 tttaatcatt tttaaagagc ccagtacttc atatccattt ctcccgctcc ttctgcagcc
     4861 ttatcaaaag gtattttaga acactcattt tagccccatt ttcatttatt atactggctt
     4921 atccaacccc tagacagagc attggcattt tccctttcct gatcttagaa gtctgatgac
     4981 tcatgaaacc agacagatta gttacataca ccacaaatcg aggctgtagc tggggcctca
     5041 acactgcagt tcttttataa ctccttagta cactttttgt tgatcctttg ccttgatcct
     5101 taattttcag tgtctatcac ctctcccgtc aggtggtgtt ccacatttgg gcctattctc
     5161 agtccaggga gttttacaac aatagatgta ttgagaatcc aacctaaagc ttaactttcc
     5221 actcccatga atgcctctct cctttttctc catttataaa ctgagctatt aaccattaat
     5281 ggtttccggg tggatgtctc ctcccccaat attacctgat gtatcttaca tattgccagg
     5341 ctgatatttt aagacattaa aaggtatatt tcattattga gccacatggt attgattact
     5401 gcttactaaa attttgtcat tgtacacatc tgtaaaaggt ggttcctttt ggaatgcaaa
     5461 gttcaggtgt ttgttgtctt tcctgaccta aggtcttgtg agcttgtatt ttttctattt
     5521 aagcagtgct ttctcttgga ctggcttgac tcatggcatt ctacacgtta ttgctggtct
     5581 aaatgtgatt ttgccaagct tcttcaggac ctataatttt gcttgacttg tagccaaaca
     5641 caagtaaaat gattaagcaa caaatgtatt tgtgaagctt ggtttttagg ttgttgtgtt
     5701 gtgtgtgctt gtgctctata ataatactat ccgggggctg gagaggtggc tcggagttca
     5761 agagcacaga ctgctcttcc agaagtcctg agttcaattc ccagcaacca catggtggct
     5821 cacaaccatc tgtaatggga tctgatgccc tcttctggtg tgtctgaaga ccacaagtgt
     5881 attcacatta aataaataaa tcctccttct tcttcttttt tttttttttt taaagagaat
     5941 actgtctcca gtagaattta ctgaagtaat gaaatacttt gtgtttgttc caatatggta
     6001 gccaataatc aaattactct ttaagcactg gaaatgttac caaggaacta atttttattt
     6061 gaagtgtaac tgtggacaga ggagccataa ctgcagactt gtgggataca gaagaccaat
     6121 gcagacttta atgtcttttc tcttacacta agcaataaag aaataaaaat tgaacttcta
     6181 gtatcctatt tgtttaaact gctagcttta cttaactttt gtgcttcatc tatacaaagc
     6241 tgaaagctaa gtctgcagcc attactaaac atgaaagcaa gtaatgataa ttttggattt
     6301 caaaaatgta gggccagagt ttagccagcc agtggtggtg cttgccttta tgcctttaat
     6361 cccagcactc tggaggcaga gacaggcaga tctctgagtt tgagcccagc ctggtctaca
     6421 catcaagttc tatctaggat agccgggaat acacacagaa accctgttgg ggaggggggc
     6481 tctgagattt cataaaatta taattgaagc attccctaat gagccactat ggatgtggct
     6541 aaatccgtct acctttctga tgagatttgg gtattatttt ttctgtctct gctgttggtt
     6601 gggtcttttg acactgtggg ctttctttaa agcctccttc ctgccatgtg gtctcttgtt
     6661 tgctactaac ttcccatggc ttaaatggca tggctttttg ccttctaagg gcagctgctg
     6721 agatttgcag cctgatttcc ggggtggggt tgggaaatct ttcaaacact aaaattgtcc
     6781 tttaattttt tttttaaaaa atgggttata taataaacct cataaaatag ttatgaggag
     6841 tgaggtggac taatattaaa tgagtccctc ccctataaaa gagctattaa ggctttttgt
     6901 cttatactta actttttttt ttaaatgtgg tatctttaga accaagggtc ttagagtttt
     6961 agtatacaga aactgttgca tcgcttaatc agattttcta gtttcaaatc cagagaatcc
     7021 aaattcttca cagccaaagt caaattaaga atttctgact tttaatgtta atttgcttac
     7081 tgtgaatata aaaatgatag cttttcctga ggcagggtct cactatgtat ctctgcctga
     7141 tctgcaacaa gatatgtaga ctaaagttct gcctgctttt gtctcctgaa tactaaggtt
     7201 aaaatgtagt aatacttttg gaacttgcag gtcagattct tttatagggg acacactaag
     7261 ggagcttggg tgatagttgg taaaatgtgt ttcaagtgat gaaaacttga attattatca
     7321 ccgcaaccta ctttttaaaa aaaaaagcca ggcctgttag agcatgctta agggatccct
     7381 aggacttgct gagcacacaa gagtagttac ttggcaggct cctggtgaga gcatatttca
     7441 aaaaacaagg cagacaacca agaaactaca gttaaggtta cctgtcttta aaccatctgc
     7501 atatacacag ggatattaaa atattccaaa taatatttca ttcaagtttt cccccatcaa
     7561 attgggacat ggatttctcc ggtgaatagg cagagttgga aactaaacaa atgttggttt
     7621 tgtgatttgt gaaattgttt tcaagtgata gttaaagccc atgagataca gaacaaagct
     7681 gctatttcga ggtctcttgg tttatactca gaagcacttc tttgggtttc cctgcactat
     7741 cctgatcatg tgctaggcct accttaggct gattgttgtt caaataaact taagtttcct
     7801 gtcaggtgat gtcatatgat ttcatatatc aaggcaaaac atgttatata tgttaaacat
     7861 ttgtacttaa tgtgaaagtt aggtctttgt gggtttgatt tttaattttc aaaacctgag
     7921 ctaaataagt catttttaca tgtcttacat ttggtggaat tgtataattg tggtttgcag
     7981 gcaagactct ctgacctagt aaccctacct atagagcact ttgctgggtc acaagtctag
     8041 gagtcaagca tttcaccttg aagttgagac gttttgttag tgtatactag tttatatgtt
     8101 ggaggacatg tttatccaga agatattcag gactattttt gactgggcta aggaattgat
     8161 tctgattagc actgttagtg agcattgagt ggcctttagg cttgaattgg agtcacttgt
     8221 atatctcaaa taatgctggc cttttttaaa aagcccttgt tctttatcac cctgttttct
     8281 acataatttt tgttcaaaga aatacttgtt tggatctcct tttgacaaca atagcatgtt
     8341 ttcaagccat attttttttc cttttttttt tttttttttg gtttttcgag acagggtttc
     8401 tctgtatagc cccggctgtc ctggaactca ctttgtagac caggctggcc tcgaactcag
     8461 aaatccgcct gcctctgcct cctgagtgcc gggattaaag gcgtgcacca ccacgcctgg
     8521 ctaagttgga tattttgtta tataactata accaatacta actccactgg gtggattttt
     8581 aattcagtca gtagtcttaa gtggtcttta ttggcccttc attaaaatct actgttcact
     8641 ctaacagagg ctgttggtac tagtggcact taagcaactt cctacggata tactagcaga
     8701 ttaagggtca gggatagaaa ctagtctagc gttttgtata cctaccagct ttatactacc
     8761 ttgttctgat agaaatattt caggacatct agcttatcga taccgtcgac ggtatcgata
     8821 agcttgatat cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     8881 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     8941 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     9001 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     9061 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     9121 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     9181 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     9241 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     9301 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagg tcctcgccat
     9361 ggatcctgat gatgttgttg attcttctaa atcttttgtg atggaaaact tttcttcgta
     9421 ccacgggact aaacctggtt atgtagattc cattcaaaaa ggtatacaaa agccaaaatc
     9481 tggtacacaa ggaaattatg acgatgattg gaaagggttt tatagtaccg acaataaata
     9541 cgacgctgcg ggatactctg tagataatga aaacccgctc tctggaaaag ctggaggcgt
     9601 ggtcaaagtg acgtatccag gactgacgaa ggttctcgca ctaaaagtgg ataatgccga
     9661 aactattaag aaagagttag gtttaagtct cactgaaccg ttgatggagc aagtcggaac
     9721 ggaagagttt atcaaaaggt tcggtgatgg tgcttcgcgt gtagtgctca gccttccctt
     9781 cgctgagggg agttctagcg ttgaatatat taataactgg gaacaggcga aagcgttaag
     9841 cgtagaactt gagattaatt ttgaaacccg tggaaaacgt ggccaagatg cgatgtatga
     9901 gtatatggct caagcctgtg caggaaatcg tgtcaggcga tctctttgtg aaggaacctt
     9961 acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa
    10021 atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt
    10081 agattccaac ctatggaact gatgaatggg agcagtggtg gaatgcagat cctagagctc
    10141 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
    10201 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
    10261 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
    10321 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
    10381 cttctgaggc ggaaagaacc agctggggct cgaggggggg cccggtaccc agcttttgtt
    10441 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
    10501 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    10561 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    10621 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    10681 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    10741 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    10801 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    10861 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    10921 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    10981 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    11041 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    11101 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    11161 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    11221 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    11281 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    11341 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    11401 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    11461 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    11521 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    11581 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    11641 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    11701 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    11761 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    11821 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    11881 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    11941 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    12001 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    12061 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    12121 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    12181 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    12241 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    12301 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    12361 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    12421 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    12481 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    12541 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    12601 ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg
    12661 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
    12721 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
    12781 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
    12841 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
    12901 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
    12961 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
    13021 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
    13081 ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    13141 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    13201 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
    13261 cgactcacta tagggcgaat tggagctcca ccgcggcagg ccctccgagc gtggtggagc
    13321 cgttctgtga gacagccggg tacgagtcgt gacgctggaa ggggcaagcg ggtggtgggc
    13381 aggaatgcgg tccgccctgc agcaaccgga gggggaggga gaagggagcg gaaaagtctc
    13441 caccggacgc ggccatggct cggggggggg gggcagcgga ggagcgcttc cggccgacgt
    13501 ctcgtcgctg attggcttct tttcctcccg ccgtgtgtga aaacacaaat ggcgtgtttt
    13561 ggttggcgta aggcgcctgt cagttaacgg cagccggagt gcgcagccgc cggcagcctc
    13621 gctctgccca ctgggtgggg cgggaggtag gtggggtgag gcgagctgga cgtgcgggcg
    13681 cggtcggcct ctggcggggc gggggagggg agggagggtc agcgaaagta gctcgcgcgc
    13741 gagcggccgc ccaccctccc cttcctctgg gggagtcgtt ttacccgccg ccggccgggc
    13801 ctcgtcgtct gattggctct cggggcccag aaaactggcc cttgccattg gctcgtgttc
    13861 gtgcaagttg agtccatccg ccggccagcg ggggcggcga ggaggcgctc ccaggttccg
    13921 gccctcccct cggccccgcg ccgcagagtc tggccgcgcg cccctgcgca acgtggcagg
    13981 aagcgcgcgc tgggggcggg gacgggcagt agggctgagc ggctgcgggg cgggtgcaag
    14041 cacgtttccg acttgagttg cctcaagagg ggcgtgctga gccagacctc catcgcgcac
    14101 tccggggagt ggagggaagg agcgagggct cagttgggct gttttggagg caggaagcac
    14161 ttgctctccc aaagtcgctc tgagttgtta tcagtaaggg agctgcagtg gagtaggcgg
    14221 ggagaaggcc gcacccttct ccggaggggg gaggggagtg ttgcaatacc tttctgggag
    14281 ttctctgctg cctcctggct tctgaggacc gccctgggcc tgggagaatc ccttccccct
    14341 cttccctcgt gatctgcaac tccagtcttt ctaggtaggg cgcagtagtc cagggtttcc
    14401 ttgatgatgt catacttatc ctgtcccttt tttttccaca gctcgcggtt gaggacaaac
    14461 tcttcgcggt ctttccagtt tcgaacacgt gggcgcgccc ccgggctagt ggatccggaa
    14521 cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtccc tcgacctgca
    14581 ggaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
    14641 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
    14701 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
    14761 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
    14821 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
    14881 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
    14941 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
    15001 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
    15061 gttctcctct tcctcatctc cgggcctttc gacctgcagc caatatggga tcggccattg
    15121 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
    15181 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
    15241 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
    15301 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
    15361 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
    15421 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
    15481 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
    15541 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
    15601 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg
    15661 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
    15721 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
    15781 tg
//
