LOCUS       Exported               10144 bp ds-DNA     circular SYN 29-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 7, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10144
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        10..385
                     /label=TRE3G promoter
     primer_bind     368..408
                     /label=Tre3g-frag1-PPM1H-Fw
     misc_feature    395..1825
                     /label=PPM1H
     primer_bind     1808..1847
                     /label=frag2-pact-fw
     primer_bind     complement(1808..1847)
                     /label=frag1-ppm1h-rv
     misc_feature    1835..2509
                     /label=PACT domain
     primer_bind     complement(2488..2530)
                     /label=frag2-lentiTO-PACT-Rv
     misc_feature    2516..2542
                     /label=HA-tag
                     /note="HA-tag"
     misc_feature    2552..3097
                     /label=EF1 promoter
     misc_feature    3097..3098
                     /label=GCTAGAC
                     /note="Geneious type: Editing History Deletion"
     gene            3104..3847
                     /label=rtta3G
     CDS             3857..3913
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     gene            3914..4312
                     /label=Blasticidin Resistance
     misc_feature    3914
                     /label=ATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTT...
                     /note="Geneious type: Editing History Deletion"
     misc_feature    4319..4908
                     /label=WPRE
     misc_feature    4682..4683
                     /label=G
                     /note="Geneious type: Editing History Deletion"
     misc_feature    complement(6759..7619)
                     /label=AmpR (beta-lactamase)
                     /note="AmpR (beta-lactamase)"
     misc_feature    9302..9534
                     /label=RRE
     misc_feature    9535..10023
                     /label=Env
     misc_feature    9535..10023
                     /label=Env
ORIGIN
        1 gatactagtt ttactcccta tcagtgatag agaacgtatg aagagtttac tccctatcag
       61 tgatagagaa cgtatgcaga ctttactccc tatcagtgat agagaacgta taaggagttt
      121 actccctatc agtgatagag aacgtatgac cagtttactc cctatcagtg atagagaacg
      181 tatctacagt ttactcccta tcagtgatag agaacgtata tccagtttac tccctatcag
      241 tgatagagaa cgtataagct ttaggcgtgt acggtgggcg cctataaaag cagagctcgt
      301 ttagtgaacc gtcagatcgc ctggagcaat tccacaacac ttttgtctta taccaacttt
      361 ccgtaccact tcctaccctc gtaaatctag aatgcgtttc ccctacgggc ggccagagtt
      421 cctggggctg tctcaggacg aggtggagtg cagcgccgac cacatcgccc gccccatcct
      481 catcctcaag gagactcggc ggctgccctg ggccactggc tacgcagagg ttatcaatgc
      541 cgggaagagc acacacaatg aagaccaagc cagctgtgag gtgctcactg tgaagaagaa
      601 ggcaggggcc gtgacctcaa ccccaaacag gaactcatcc aagagacggt cctcccttcc
      661 caatggggaa gggctgcagc tgaaggagaa ctcggaatcc gagggtgttt cctgccacta
      721 ttggtcgctg tttgacgggc acgcggggtc cggggccgcg gtggtggcgt cacgcctgct
      781 gcagcaccac atcacggagc agctgcagga catcgtggac atcctgaaga actccgccgt
      841 cctgccccct acctgcctgg gggaggagcc tgagaacacg cccgccaaca gccggactct
      901 gacccgggca gcctccctgc gcggaggggt gggggccccg ggctccccca gcacgccccc
      961 cacacgcttc tttaccgaga agaagattcc ccatgagtgc ctggtcatcg gagcgcttga
     1021 aagtgcattc aaggaaatgg acctacagat agaacgagag aggagttcat ataatatatc
     1081 tggtggctgc acggccctca ttgtgatttg ccttttgggg aagctgtatg ttgcaaatgc
     1141 tggggatagc agggccataa tcatcagaaa tggagaaatt atccccatgt cttcagaatt
     1201 tacccccgag acggagcgcc agcgacttca gtacctggca ttcatgcagc ctcacttgct
     1261 gggaaatgag ttcacacatt tggagtttcc aaggagagta cagagaaagg agcttggaaa
     1321 gaagatgctc tacagggact ttaatatgac aggctgggca tacaaaacca ttgaggatga
     1381 ggacttgaag ttccccctta tatatggaga aggcaagaag gcccgggtaa tggcaactat
     1441 tggagtgacc aggggacttg gggaccatga cctgaaggtg catgactcca acatctacat
     1501 taaaccattc ctgtcttcag ctccagaggt aagaatctac gatctttcaa aatatgatca
     1561 tggatcagat gatgtgctga tcttggccac tgatggactc tgggacgttt tatcaaatga
     1621 agaagtagca gaagcaatca ctcagtttct tcctaactgt gatccagatg atcctcacag
     1681 gtacacactg gcagctcagg acctggtgat gcgtgcccgg ggtgtgctga aggacagagg
     1741 atggcggata tctaatgacc gactgggctc aggagacgac atttctgtat atgtcattcc
     1801 tttaatacat ggaaacaagc tgtcagccgc cgccgacccc ggccggcttc caccagctgc
     1861 cagcgaggaa gcacacacca gcaatgtcaa gatggaaaaa ttgtacctgc attacttgag
     1921 agcagagagc tttagaaaag ctctgattta tcaaaagaag tatcttttgc tgttgattgg
     1981 tggattccag gattctgaac aagaaacact ctccatgatt gcccatttgg gggtatttcc
     2041 ttccaaagca gaacggaaaa tcacatctcg tcctttcacc aggttccgca cggccgtcag
     2101 ggtggtcatt gcaatattaa gattacgttt tttggttaag aaatggcaag aagtagatcg
     2161 gaaaggagct ctggcacaag gcaaagcccc tcgcccaggg ccccgagcac gacagccgca
     2221 gtctccaccc agaaccagag agtccccccc aacccgggat gtaccctctg gccacaccag
     2281 ggaccctgcc agaggccgca gactggcagc agcagcctcc ccacacagtg ggggaagagc
     2341 cactccatcc ccaaattcaa gattagaaag atccctgact gcttctcaag atccagaaca
     2401 ttccttgaca gagtatattc accatttaga agtgatccag caaagattgg gaggggtact
     2461 accagattct acttcaaaga aatcctgcca cccgatgatt aaacagtgtg ctagctaccc
     2521 ttacgacgtg ccagactacg cctaaggccg caaggatctg cgatcgctcc ggtgcccgtc
     2581 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2641 gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2701 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2761 ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct
     2821 ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc
     2881 tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc
     2941 tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc
     3001 tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc
     3061 tgcgccgtta cagatccaag ctgtgaccgg cgcctacgcc accatgagcc ggctggacaa
     3121 gagcaaagtg atcaacagcg ccctggaact gctgaacggc gtgggcatcg agggcctgac
     3181 cacccggaag ctggcccaga aactgggcgt ggaacagccc accctgtact ggcacgtgaa
     3241 gaacaagcgg gccctgctgg acgccctgcc catcgagatg ctggaccggc accacaccca
     3301 cagctgccct ctggaaggcg agagctggca ggacttcctg cggaacaacg ccaagagcta
     3361 cagatgcgcc ctgctgagcc accgggacgg cgccaaagtg cacctgggca ccagacccac
     3421 cgagaagcag tacgagacac tggaaaacca gctggccttc ctgtgccagc agggcttcag
     3481 cctggaaaac gccctgtacg ccctgagcgc cgtgggccac ttcaccctgg gctgcgtgct
     3541 ggaagaacag gaacaccagg tcgccaaaga ggaaagagag acacccacca ccgacagcat
     3601 gccccccctg ctgaagcagg ccatcgagct gttcgacaga cagggcgccg agcccgcctt
     3661 cctgttcggc ctggaactga tcatctgcgg cctcgagaag cagctgaagt gcgagagcgg
     3721 cggacccacc gacgccctgg acgacttcga cctggacatg ctgcccgccg atgccctgga
     3781 tgattttgat ctggatatgc tccctgctga cgctctcgat gacttcgatc tcgatatgct
     3841 gccaggcgga agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga
     3901 gaaccctgga cctatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     3961 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
     4021 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     4081 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     4141 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     4201 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     4261 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct aagtcgacaa
     4321 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4381 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4441 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     4501 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     4561 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     4621 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4681 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     4741 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4801 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4861 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
     4921 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     4981 ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct
     5041 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5101 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5161 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     5221 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     5281 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5341 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5401 atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct
     5461 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5521 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc
     5581 ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5641 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5701 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5761 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5821 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5881 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5941 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6001 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6061 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6121 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6181 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6241 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6301 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6361 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6421 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6481 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6541 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6601 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6661 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6721 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6781 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6841 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6901 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6961 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7021 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7081 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7141 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7201 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7261 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7321 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7381 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7441 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7501 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7561 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7621 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7681 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7741 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     7801 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     7861 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     7921 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     7981 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     8041 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     8101 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     8161 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
     8221 ctgacgcgtg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     8281 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     8341 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     8401 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca ataaacgggt
     8461 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8521 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8581 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     8641 gcgcccgaac agggacctga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     8701 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     8761 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     8821 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     8881 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     8941 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     9001 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     9061 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     9121 caaaagtaag accaccgcac agcaagcggc cactgatctt cagacctgga ggaggagata
     9181 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     9241 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     9301 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcctcaa
     9361 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     9421 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     9481 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     9541 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     9601 gtaataaatc tctggaacag attggaatca cacgacctgg atggagtggg acagagaaat
     9661 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     9721 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     9781 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     9841 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     9901 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     9961 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggtt
    10021 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
    10081 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
    10141 tatc
//
